EC: 2.7.7.23

UDP-N-acetylglucosamine diphosphorylase (UTP:N-acetyl-alpha-D-glucosamine-1-phosphate uridylyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q940S3 [Mg2+]

N_Acetyl_D_glucosamine_1_phosphate + UTP -> UDP_N_acetyl_D_glucosamine + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl, 40 U/ml yeast inorganic pyrophosphatase
PH: 7.6
Temperature: 37
uniprot:Q940S3 [Mg2+]

N_Acetyl_D_glucosamine_1_phosphate + UTP -> Diphosphate + UDP_N_acetyl_D_glucosamine		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 M Tris/HCl, 40 U/ml yeast inorganic pyrophosphatase
PH: 7.6
Temperature: 37
uniprot:O64765 [Mg2+]

alpha_D_Glucose_1_phosphate + UTP -> UDP_D_glucose + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl, 40 U/ml yeast inorganic pyrophosphatase
PH: 7.6
Temperature: 37
uniprot:Q940S3 [Mg2+]

UDP_N_acetyl_D_glucosamine + Diphosphate -> N_Acetyl_D_glucosamine_1_phosphate + UTP		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 7.6
Temperature: 37
uniprot:O64765 [Mg2+]

UDP_N_acetyl_D_glucosamine + Diphosphate -> UTP + N_Acetyl_D_glucosamine_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 7.6
Temperature: 37
uniprot:O64765 [Mg2+]

N_Acetyl_D_glucosamine_1_phosphate + UTP -> Diphosphate + UDP_N_acetyl_D_glucosamine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl, 40 U/ml yeast inorganic pyrophosphatase
PH: 7.6
Temperature: 37
uniprot:O64765 [Mg2+]

N_Acetyl_D_glucosamine_1_phosphate + UTP -> UDP_N_acetyl_D_glucosamine + Diphosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 M Tris/HCl, 40 U/ml yeast inorganic pyrophosphatase
PH: 7.6
Temperature: 37
uniprot:O64765 [Mg2+]

Diphosphate + UDP_N_acetyl_D_galactosamine -> UTP + N_Acetyl_D_galactosamine_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 7.6
Temperature: 37
uniprot:Q940S3 [Mg2+]

UDP_N_acetyl_D_galactosamine + Diphosphate -> UTP + N_Acetyl_D_galactosamine_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 7.6
Temperature: 37
- [UDP-N-acetylglucosamine diphosphorylase(Enzyme) wildtype]

UTP + D_Glucose_1_phosphate -> UDPglucose + Diphosphate		 (( (V * A) ) / (Km + A)) buffer: 80 mM Tris-HCl, 5.0 mM MgCl2, 5.0 mM NAD+, 2.67 U/ml UDPglucose dehydrogenase
PH: 7.5
Temperature: 36
uniprot:P0ACC7 [Mg2+]

N_Acetyl_D_glucosamine_1_phosphate + UTP -> Diphosphate + UDP_N_acetyl_D_glucosamine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 8.2
Temperature: 37
uniprot:P0ACC7 [Mg2+]

UTP + N_Acetyl_D_glucosamine_1_phosphate -> UDP_N_acetyl_D_glucosamine + Diphosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl
PH: 8.2
Temperature: 37

Pathways

pathway id name
BioCyc:META_SUCSYN-PWY sucrose biosynthesis I (from photosynthesis)
BioCyc:YEAST_UDPNAGSYN-YEAST-PWY UDP-N-acetylglucosamine biosynthesis
BioCyc:YEAST_PWY-6981 chitin biosynthesis
BioCyc:ARA_PWYQT-4466 superpathway of sucrose and starch metabolism I (non-photosynthetic tissue)
BioCyc:ECOO157_UDPNAGSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis I
BioCyc:AGRO_UDPNAGSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis I
BioCyc:SYNEL_PWY0-1261 anhydromuropeptides recycling
BioCyc:CAULONA1000_UDPNAGSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis I
BioCyc:GCF_000013425_UDPNAGSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis I
BioCyc:HUMAN_UDPNACETYLGALSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis II
BioCyc:META_PWY-6749 CMP-legionaminate biosynthesis I
BioCyc:META_OANTIGEN-PWY O-antigen building blocks biosynthesis (E. coli)
BioCyc:META_PWY0-1261 anhydromuropeptides recycling I
BioCyc:META_UDPNACETYLGALSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis II
BioCyc:META_UDPNAGSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis I
BioCyc:META_PWY-8013 UDP-N-acetyl-D-galactosamine biosynthesis III
BioCyc:META_PWY-7343 UDP-α-D-glucose biosynthesis I
BioCyc:META_PWY-7345 superpathway of anaerobic sucrose degradation
BioCyc:VCHO_UDPNAGSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis I
BioCyc:MTBH37RV_PWY-6404 superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis
BioCyc:CAULO_OANTIGEN-PWY O-antigen building blocks biosynthesis (E. coli)
BioCyc:AGRO_OANTIGEN-PWY O-antigen building blocks biosynthesis (E. coli)
BioCyc:BSUB_UDPNAGSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis I
BioCyc:ECOL316407_OANTIGEN-PWY O-antigen building blocks biosynthesis (E. coli)
BioCyc:ECOL316407_PWY0-1261 anhydromuropeptides recycling
BioCyc:CAULONA1000_OANTIGEN-PWY O-antigen biosynthesis (E. coli)
BioCyc:CORYNE_UDPNAGSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis I
BioCyc:META_PWY-7238 sucrose biosynthesis II
BioCyc:META_PWY-3801 sucrose degradation II (sucrose synthase)
BioCyc:META_PWY-7332 superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis
BioCyc:ARA_PWY-3801 sucrose degradation II (sucrose synthase)
BioCyc:ARA_PWY-6527 stachyose degradation
BioCyc:MTBH37RV_UDPNAGSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis I
BioCyc:HPY_UDPNAGSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis I
BioCyc:ECOO157_PWY0-1261 anhydromuropeptides recycling
BioCyc:CAULO_UDPNAGSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis I
BioCyc:MOUSE_UDPNACETYLGALSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis II
BioCyc:MOUSE_PWY-5514 UDP-N-acetyl-D-galactosamine biosynthesis II
BioCyc:PDIF272563_PWY0-1261 anhydromuropeptides recycling
BioCyc:CORYNE_PWY0-1261 anhydromuropeptides recycling
BioCyc:HUMAN_PWY-5514 UDP-N-acetyl-D-galactosamine biosynthesis II
BioCyc:META_PWY-6981 chitin biosynthesis
BioCyc:ECO_PWY0-1261 anhydromuropeptides recycling I
BioCyc:ECO_OANTIGEN-PWY O-antigen building blocks biosynthesis (E. coli)
BioCyc:ECO_UDPNAGSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis I
BioCyc:YEAST_UDPNACETYLGALSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis II
BioCyc:ARA_PWY-7238 sucrose biosynthesis II
BioCyc:ARA_PWY-7343 UDP-glucose biosynthesis
BioCyc:ARA_UDPNACETYLGALSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis II
BioCyc:SYNEL_UDPNAGSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis I
BioCyc:SYNEL_OANTIGEN-PWY O-antigen building blocks biosynthesis (E. coli)
BioCyc:ECOL316407_UDPNAGSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis I
BioCyc:PDIF272563_UDPNAGSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis I
PathBank:SMP0000851 Lipopolysaccharide Biosynthesis
PathBank:SMP0000905 Amino Sugar and Nucleotide Sugar Metabolism I
PathBank:SMP0000924 Peptidoglycan Biosynthesis I
PathBank:SMP0001919 Lipopolysaccharide Biosynthesis II
PathBank:SMP0002071 Lipopolysaccharide Biosynthesis III
PathBank:SMP0002074 Peptidoglycan Biosynthesis II
PathBank:SMP0002076 1,6-Anhydro-N-acetylmuramic Acid Recycling
PathBank:SMP0002101 O-Antigen Building Blocks Biosynthesis
PlantCyc:ARA_PWY-6527 stachyose degradation
PlantCyc:ARA_PWY-7345 superpathway of anaerobic sucrose degradation
PlantCyc:PLANT_PWY-7343 UDP-α-D-glucose biosynthesis I
PlantCyc:PLANT_UDPNACETYLGALSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis II
PlantCyc:PLANT_PWY-7238 sucrose biosynthesis II
PlantCyc:PLANT_SUCSYN-PWY sucrose biosynthesis I (from photosynthesis)
PlantCyc:ARA_PWY-7343 UDP-α-D-glucose biosynthesis I
PlantCyc:ARA_UDPNACETYLGALSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis II
PlantCyc:ARA_PWY-7238 sucrose biosynthesis II
PlantCyc:ARA_PWY-3801 sucrose degradation II (sucrose synthase)
PlantCyc:PLANT_PWY-7345 superpathway of anaerobic sucrose degradation
PlantCyc:PLANT_PWY-3801 sucrose degradation II (sucrose synthase)
PlantCyc:ARA_PWYQT-4466 superpathway of sucrose and starch metabolism I (non-photosynthetic tissue)
PlantCyc:ARA_SUCSYN-PWY sucrose biosynthesis I (from photosynthesis)
PlantCyc:PLANT_PWYQT-4466 superpathway of sucrose and starch metabolism I (non-photosynthetic tissue)
Reactome:R-CEL-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-DDI-597592 Post-translational protein modification
Reactome:R-DDI-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-MMU-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-SCE-597592 Post-translational protein modification
Reactome:R-SCE-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-SPO-446203 Asparagine N-linked glycosylation
Reactome:R-SPO-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-CEL-392499 Metabolism of proteins
Reactome:R-CEL-597592 Post-translational protein modification
Reactome:R-CEL-446203 Asparagine N-linked glycosylation
Reactome:R-CEL-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-DDI-392499 Metabolism of proteins
Reactome:R-DDI-446203 Asparagine N-linked glycosylation
Reactome:R-DDI-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-HSA-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-MMU-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-SCE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-CEL-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-DDI-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-HSA-446203 Asparagine N-linked glycosylation
Reactome:R-HSA-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-SPO-392499 Metabolism of proteins
Reactome:R-SPO-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-HSA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-MMU-446203 Asparagine N-linked glycosylation
Reactome:R-MMU-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-SCE-392499 Metabolism of proteins
Reactome:R-SCE-446203 Asparagine N-linked glycosylation
Reactome:R-SCE-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-SPO-597592 Post-translational protein modification
Reactome:R-SPO-446219 Synthesis of substrates in N-glycan biosythesis
WikiPathways:WP5220 Metabolic reprogramming in pancreatic cancer
PathBank:SMP0122112 Lipopolysaccharide Biosynthesis II
PathBank:SMP0122240 1,6-Anhydro-N-acetylmuramic Acid Recycling
PathBank:SMP0000240 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0002331 Amino Sugar and Nucleotide Sugar Metabolism
PathBank:SMP0121299 Lipopolysaccharide Biosynthesis
PathBank:SMP0122239 Peptidoglycan Biosynthesis II
PathBank:SMP0000045 Amino Sugar Metabolism
PathBank:SMP0000216 Sialuria or French Type Sialuria
PathBank:SMP0121305 Peptidoglycan Biosynthesis I
PathBank:SMP0122237 Lipopolysaccharide Biosynthesis III
PathBank:SMP0122253 O-Antigen Building Blocks Biosynthesis
PathBank:SMP0000390 Tay-Sachs Disease
PathBank:SMP0121302 Amino Sugar and Nucleotide Sugar Metabolism I
PathBank:SMP0000534 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0094667 Chitin Biosynthesis