EC: 2.7.1.91

sphingosine kinase (ATP:sphingoid base 1-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q9JIA7 [KCl]

ATP + Sphingosine -> Sphingosine_1_phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
uniprot:Q9JIA7 [KCl]

ATP + Sphingosine -> ADP + Sphingosine_1_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
uniprot:Q9JIA7 [KCl]

Sphingosine + ATP -> Sphingosine_1_phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
uniprot:Q9NYA1 [sphinganine kinase(Enzyme) wildtype]

Sphingosine + ATP -> Sphingosine_1_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 10% glycerol, 0.5 M NaCl, BSA
PH: 7.4
Temperature: 37
uniprot:Q9NYA1 [sphinganine kinase(Enzyme) wildtype]

Sphingosine + ATP -> ADP + Sphingosine_1_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 10% glycerol, 0.5 M NaCl, BSA
PH: 7.4
Temperature: 37
uniprot:Q9NYA1 [sphinganine kinase(Enzyme) wildtype]

Sphingosine + ATP -> Sphingosine_1_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 10% glycerol, 0.5 M NaCl, 0.25% (w/v) Triton X-100
PH: 7.4
Temperature: 37
uniprot:Q9NYA1 [sphinganine kinase(Enzyme) wildtype]

Sphinganine + ATP -> Sphinganine_1_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 10% glycerol, 0.5 M NaCl, 0.25% (w/v) Triton X-100
PH: 7.4
Temperature: 37
uniprot:Q9NYA1 [sphinganine kinase(Enzyme) wildtype]

Sphingosine + ATP -> Sphingosine_1_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 10% glycerol, 0.5 M NaCl, 0.25% (w/v) Triton X-100
PH: 7.4
Temperature: 37
uniprot:Q9NYA1 [sphinganine kinase(Enzyme) wildtype]

Sphinganine + ATP -> Sphinganine_1_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 10% glycerol, 0.5 M NaCl, 0.25% (w/v) Triton X-100
PH: 7.4
Temperature: 37
uniprot:Q9NYA1 [sphinganine kinase(Enzyme) wildtype]

ATP + Sphingosine -> Sphingosine_1_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 10% glycerol, 0.5 M NaCl, 0.25% (w/v) Triton X-100
PH: 7.4
Temperature: 37
uniprot:Q9NYA1 [sphinganine kinase(Enzyme) wildtype]

ATP + Sphingosine -> Sphingosine_1_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 10% glycerol, 0.5 M NaCl, 0.25% (w/v) Triton X-100
PH: 7.4
Temperature: 37
uniprot:Q8L7L1 [Mg2+]

ATP + Phytosphingosine -> ADP + Phytosphingosine_1_phosphate		 (( (( (Vmax * A) ) * B) ) / (( (( (Ks_A * Km_B) ) + (Km_B * A)) ) + (A * B))) buffer: 20 mM Tris, 20% glycerol, 1 mM mercaptoethanol, 1 mM EDTA, 1 mM sodium orthovanadate, 40 mM beta-glycerophosphate, 15 mM NaF, 10 mg/ml leupeptin, 10 mg/ml aprotinin, 10 mg/ml soybean trypsin inhibitor, 1 mM phenylmethylsulfonyl fluoride, 0.5 mM 4-deoxypyridoxine
PH: 7.4
Temperature: 37
uniprot:Q8L7L1 [1,2-Diacyl-sn-glycerol 3-phosphate]

Phytosphingosine + ATP -> ADP + Phytosphingosine_1_phosphate		 (( (( (Vmax * A) ) * B) ) / (( (( (Ks_A * Km_B) ) + (Km_B * A)) ) + (A * B))) buffer: 20 mM Tris, 20% glycerol, 1 mM mercaptoethanol, 1 mM EDTA, 1 mM sodium orthovanadate, 40 mM beta-glycerophosphate, 15 mM NaF, 10 mg/ml leupeptin, 10 mg/ml aprotinin, 10 mg/ml soybean trypsin inhibitor, 1 mM phenylmethylsulfonyl fluoride, 0.5 mM 4-deoxypyridoxine
PH: 7.4
Temperature: 37
uniprot:Q8L7L1 [1,2-Diacyl-sn-glycerol 3-phosphate]

ATP + Phytosphingosine -> ADP + Phytosphingosine_1_phosphate		 (( (( (Vmax * A) ) * B) ) / (( (( (Ks_A * Km_B) ) + (Km_B * A)) ) + (A * B))) buffer: 20 mM Tris, 20% glycerol, 1 mM mercaptoethanol, 1 mM EDTA, 1 mM sodium orthovanadate, 40 mM beta-glycerophosphate, 15 mM NaF, 10 mg/ml leupeptin, 10 mg/ml aprotinin, 10 mg/ml soybean trypsin inhibitor, 1 mM phenylmethylsulfonyl fluoride, 0.5 mM 4-deoxypyridoxine
PH: 7.4
Temperature: 37
uniprot:Q8L7L1 [1,2-Diacyl-sn-glycerol 3-phosphate]

Phytosphingosine + ATP -> Phytosphingosine_1_phosphate + ADP		 (( (( (Vmax * A) ) * B) ) / (( (( (Ks_A * Km_B) ) + (Km_B * A)) ) + (A * B))) buffer: 20 mM Tris, 20% glycerol, 1 mM mercaptoethanol, 1 mM EDTA, 1 mM sodium orthovanadate, 40 mM beta-glycerophosphate, 15 mM NaF, 10 mg/ml leupeptin, 10 mg/ml aprotinin, 10 mg/ml soybean trypsin inhibitor, 1 mM phenylmethylsulfonyl fluoride, 0.5 mM 4-deoxypyridoxine
PH: 7.4
Temperature: 37
uniprot:Q8L7L1 [1,2-Diacyl-sn-glycerol 3-phosphate]

ATP + Phytosphingosine -> Phytosphingosine_1_phosphate + ADP		 (( (( (Vmax * A) ) * B) ) / (( (( (Ks_A * Km_B) ) + (Km_B * A)) ) + (A * B))) buffer: 20 mM Tris, 20% glycerol, 1 mM mercaptoethanol, 1 mM EDTA, 1 mM sodium orthovanadate, 40 mM beta-glycerophosphate, 15 mM NaF, 10 mg/ml leupeptin, 10 mg/ml aprotinin, 10 mg/ml soybean trypsin inhibitor, 1 mM phenylmethylsulfonyl fluoride, 0.5 mM 4-deoxypyridoxine
PH: 7.4
Temperature: 37
uniprot:Q8L7L1 [Mg2+]

Phytosphingosine + ATP -> ADP + Phytosphingosine_1_phosphate		 (( (( (Vmax * A) ) * B) ) / (( (( (Ks_A * Km_B) ) + (Km_B * A)) ) + (A * B))) buffer: 20 mM Tris, 20% glycerol, 1 mM mercaptoethanol, 1 mM EDTA, 1 mM sodium orthovanadate, 40 mM beta-glycerophosphate, 15 mM NaF, 10 mg/ml leupeptin, 10 mg/ml aprotinin, 10 mg/ml soybean trypsin inhibitor, 1 mM phenylmethylsulfonyl fluoride, 0.5 mM 4-deoxypyridoxine
PH: 7.4
Temperature: 37
uniprot:Q91V26 [Mg2+]

Sphingosine + ATP -> ADP + Sphingosine_1_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 20% glycerol, 1 mM mercaptoethanol, 1 mM EDTA, 1 mM sodium orthovanadate, 40 mM beta-glycerophosphate, 15 mM NaF, 10 µg/ml leupeptin, 10 µg/ml aprotinin, 10 µg/ml soybean trypsin inhibitor, 1 mM phenylmethylsulfonyl fluoride, 0.5 mM 4-deoxypyridoxine
PH: 7.4
Temperature: 37
uniprot:Q91V26 [Mg2+]

ATP + Sphingosine -> ADP + Sphingosine_1_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 20% glycerol, 1 mM mercaptoethanol, 1 mM EDTA, 1 mM sodium orthovanadate, 40 mM beta-glycerophosphate, 15 mM NaF, 10 µg/ml leupeptin, 10 µg/ml aprotinin, 10 µg/ml soybean trypsin inhibitor, 1 mM phenylmethylsulfonyl fluoride, 0.5 mM 4-deoxypyridoxine
PH: 7.4
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY3DJ-11470 sphingosine and sphingosine-1-phosphate metabolism
BioCyc:YEAST_PWY-7119 sphingolipid recycling and degradation (yeast)
BioCyc:META_PWY3DJ-11470 sphingosine and sphingosine-1-phosphate metabolism
BioCyc:ARA_PWY-5129 sphingolipid biosynthesis (plants)
BioCyc:META_PWY-7119 sphingolipid recycling and degradation (yeast)
BioCyc:META_PWY-5129 sphingolipid biosynthesis (plants)
BioCyc:ARA_PWY-6483 ceramide degradation
BioCyc:MOUSE_PWY3DJ-11470 sphingosine and sphingosine-1-phosphate metabolism
BioCyc:META_PWY-6483 ceramide degradation
PlantCyc:ARA_PWY-6483 ceramide degradation
PlantCyc:ARA_PWY-5129 sphingolipid biosynthesis (plants)
PlantCyc:PLANT_PWY-5129 sphingolipid biosynthesis (plants)
PlantCyc:PLANT_PWY-6483 ceramide degradation
Reactome:R-CEL-9833482 PKR-mediated signaling
Reactome:R-RNO-9833482 PKR-mediated signaling
Reactome:R-MMU-9833482 PKR-mediated signaling
Reactome:R-HSA-9833482 PKR-mediated signaling
Reactome:R-RNO-9009391 Extra-nuclear estrogen signaling
Reactome:R-HSA-9009391 Extra-nuclear estrogen signaling
Reactome:R-CEL-9009391 Extra-nuclear estrogen signaling
Reactome:R-MMU-9009391 Extra-nuclear estrogen signaling
Reactome:R-CEL-390466 Chaperonin-mediated protein folding
Reactome:R-CEL-4420097 VEGFA-VEGFR2 Pathway
Reactome:R-CEL-428157 Sphingolipid metabolism
Reactome:R-DDI-1483206 Glycerophospholipid biosynthesis
Reactome:R-HSA-1660661 Sphingolipid de novo biosynthesis
Reactome:R-HSA-5218921 VEGFR2 mediated cell proliferation
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-390466 Chaperonin-mediated protein folding
Reactome:R-HSA-1169410 Antiviral mechanism by IFN-stimulated genes
Reactome:R-MMU-168256 Immune System
Reactome:R-MMU-913531 Interferon Signaling
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-162582 Signaling Pathways
Reactome:R-MMU-8939211 ESR-mediated signaling
Reactome:R-RNO-391251 Protein folding
Reactome:R-RNO-162582 Signaling Pathways
Reactome:R-RNO-4420097 VEGFA-VEGFR2 Pathway
Reactome:R-RNO-9006931 Signaling by Nuclear Receptors
Reactome:R-RNO-1169410 Antiviral mechanism by IFN-stimulated genes
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-1483206 Glycerophospholipid biosynthesis
Reactome:R-CEL-168256 Immune System
Reactome:R-CEL-913531 Interferon Signaling
Reactome:R-CEL-1169410 Antiviral mechanism by IFN-stimulated genes
Reactome:R-CEL-392499 Metabolism of proteins
Reactome:R-CEL-391251 Protein folding
Reactome:R-CEL-194138 Signaling by VEGF
Reactome:R-CEL-9006931 Signaling by Nuclear Receptors
Reactome:R-CEL-556833 Metabolism of lipids
Reactome:R-DDI-1483257 Phospholipid metabolism
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-428157 Sphingolipid metabolism
Reactome:R-HSA-9006934 Signaling by Receptor Tyrosine Kinases
Reactome:R-HSA-194138 Signaling by VEGF
Reactome:R-HSA-9006931 Signaling by Nuclear Receptors
Reactome:R-HSA-391251 Protein folding
Reactome:R-HSA-913531 Interferon Signaling
Reactome:R-MMU-1280215 Cytokine Signaling in Immune system
Reactome:R-MMU-5218921 VEGFR2 mediated cell proliferation
Reactome:R-MMU-390471 Association of TriC/CCT with target proteins during biosynthesis
Reactome:R-RNO-1660661 Sphingolipid de novo biosynthesis
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-RNO-8939211 ESR-mediated signaling
Reactome:R-RNO-168256 Immune System
Reactome:R-RNO-913531 Interferon Signaling
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-1483206 Glycerophospholipid biosynthesis
Reactome:R-SPO-1483257 Phospholipid metabolism
Reactome:R-CEL-390471 Association of TriC/CCT with target proteins during biosynthesis
Reactome:R-CEL-162582 Signaling Pathways
Reactome:R-CEL-1660661 Sphingolipid de novo biosynthesis
Reactome:R-HSA-390471 Association of TriC/CCT with target proteins during biosynthesis
Reactome:R-HSA-168256 Immune System
Reactome:R-MMU-1169410 Antiviral mechanism by IFN-stimulated genes
Reactome:R-MMU-428157 Sphingolipid metabolism
Reactome:R-MMU-9006934 Signaling by Receptor Tyrosine Kinases
Reactome:R-MMU-4420097 VEGFA-VEGFR2 Pathway
Reactome:R-MMU-9006931 Signaling by Nuclear Receptors
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-391251 Protein folding
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-390466 Chaperonin-mediated protein folding
Reactome:R-RNO-9006934 Signaling by Receptor Tyrosine Kinases
Reactome:R-RNO-194138 Signaling by VEGF
Reactome:R-SCE-556833 Metabolism of lipids
Reactome:R-CEL-1280215 Cytokine Signaling in Immune system
Reactome:R-CEL-9006934 Signaling by Receptor Tyrosine Kinases
Reactome:R-CEL-5218921 VEGFR2 mediated cell proliferation
Reactome:R-CEL-8939211 ESR-mediated signaling
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-556833 Metabolism of lipids
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-162582 Signaling Pathways
Reactome:R-HSA-4420097 VEGFA-VEGFR2 Pathway
Reactome:R-HSA-8939211 ESR-mediated signaling
Reactome:R-HSA-1280215 Cytokine Signaling in Immune system
Reactome:R-MMU-1660661 Sphingolipid de novo biosynthesis
Reactome:R-MMU-194138 Signaling by VEGF
Reactome:R-MMU-390466 Chaperonin-mediated protein folding
Reactome:R-RNO-428157 Sphingolipid metabolism
Reactome:R-RNO-390471 Association of TriC/CCT with target proteins during biosynthesis
Reactome:R-RNO-5218921 VEGFR2 mediated cell proliferation
Reactome:R-RNO-1280215 Cytokine Signaling in Immune system
Reactome:R-SCE-1483257 Phospholipid metabolism
Reactome:R-SPO-556833 Metabolism of lipids
WikiPathways:WP4690 Sphingolipid metabolism (integrated pathway)
WikiPathways:WP4344 Sphingolipid metabolism overview
PathBank:SMP0000349 Gaucher Disease
PathBank:SMP0063667 Sphingolipid Metabolism
PathBank:SMP0120481 Gaucher Disease
PathBank:SMP0120590 Fabry Disease
PathBank:SMP0000348 Globoid Cell Leukodystrophy
PathBank:SMP0000525 Fabry Disease
PathBank:SMP0120482 Globoid Cell Leukodystrophy
PathBank:SMP0120518 Metachromatic Leukodystrophy (MLD)
PathBank:SMP0120591 Krabbe Disease
PathBank:SMP0000034 Sphingolipid Metabolism
PathBank:SMP0000347 Metachromatic Leukodystrophy (MLD)
PathBank:SMP0000526 Krabbe Disease