EC: 2.4.2.14

amidophosphoribosyltransferase (5-phospho-beta-D-ribosylamine:diphosphate phospho-alpha-D-ribosyltransferase (glutamate-amidating))

enzyme lambda metabolic reaction experiment
uniprot:P35433 [amidophosphoribosyltransferase(Enzyme) wildtype]

L_Glutamine + H2O + 5_Phospho_alpha_D_ribose_1_diphosphate -> L_Glutamate + Diphosphate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * S1) ) / (Km + S1)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 1 mM Potassium fluoride
PH: 7.2
Temperature: 37
uniprot:P35433 [amidophosphoribosyltransferase(Enzyme) wildtype]

L_Glutamine + H2O + 5_Phospho_alpha_D_ribose_1_diphosphate -> 5_Phospho_beta_D_ribosylamine + Diphosphate + L_Glutamate		 (( (Vmax * S2) ) / (Km + S2)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 1 mM Potassium fluoride
PH: 7.2
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant R73L/Y74L]

H2O + L_Glutamine + 5_Phospho_alpha_D_ribose_1_diphosphate -> 5_Phospho_beta_D_ribosylamine + L_Glutamate + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant Y74F]

H2O + 5_Phospho_alpha_D_ribose_1_diphosphate + L_Glutamine -> 5_Phospho_beta_D_ribosylamine + Diphosphate + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant R73H]

5_Phospho_alpha_D_ribose_1_diphosphate + H2O + L_Glutamine -> L_Glutamate + Diphosphate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant R73L]

L_Glutamine + H2O + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + 5_Phospho_beta_D_ribosylamine + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant Y74L]

L_Glutamine + H2O -> NH3 + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant Y74S]

H2O + L_Glutamine -> NH3 + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant Y74F]

H2O + L_Glutamine -> NH3 + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant R73L]

L_Glutamine + H2O -> L_Glutamate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant R73H]

L_Glutamine + H2O -> L_Glutamate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant R73L]

H2O + 5_Phospho_alpha_D_ribose_1_diphosphate + L_Glutamine -> Diphosphate + 5_Phospho_beta_D_ribosylamine + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) wildtype]

L_Glutamine + 5_Phospho_alpha_D_ribose_1_diphosphate + H2O -> L_Glutamate + 5_Phospho_beta_D_ribosylamine + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant R73H]

5_Phospho_alpha_D_ribose_1_diphosphate + H2O + NH3 -> 5_Phospho_beta_D_ribosylamine + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8.5
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant N101D]

NH3 + 5_Phospho_alpha_D_ribose_1_diphosphate + H2O -> Diphosphate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8.5
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant D127A]

H2O + NH3 + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8.5
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant R73L]

H2O + 5_Phospho_alpha_D_ribose_1_diphosphate + NH3 -> Diphosphate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8.5
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant G102A]

5_Phospho_alpha_D_ribose_1_diphosphate + NH3 + H2O -> 5_Phospho_beta_D_ribosylamine + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8.5
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant N101G]

NH3 + H2O + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8.5
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant Ala insert]

NH3 + 5_Phospho_alpha_D_ribose_1_diphosphate + H2O -> 5_Phospho_beta_D_ribosylamine + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8.5
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) wildtype]

L_Glutamine + 5_Phospho_alpha_D_ribose_1_diphosphate + H2O -> L_Glutamate + Diphosphate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) wildtype]

H2O + 5_Phospho_alpha_D_ribose_1_diphosphate + L_Glutamine -> L_Glutamate + 5_Phospho_beta_D_ribosylamine + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant Y74S]

H2O + 5_Phospho_alpha_D_ribose_1_diphosphate + L_Glutamine -> 5_Phospho_beta_D_ribosylamine + Diphosphate + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) wildtype]

H2O + L_Glutamine + 5_Phospho_alpha_D_ribose_1_diphosphate -> 5_Phospho_beta_D_ribosylamine + Diphosphate + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant Y74L]

5_Phospho_alpha_D_ribose_1_diphosphate + H2O + L_Glutamine -> Diphosphate + L_Glutamate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) wildtype]

L_Glutamine + H2O -> L_Glutamate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) wildtype]

NH3 + 5_Phospho_alpha_D_ribose_1_diphosphate + H2O -> Diphosphate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8.5
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant Ala insert]

H2O + L_Glutamine + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + L_Glutamate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant N101D]

5_Phospho_alpha_D_ribose_1_diphosphate + L_Glutamine + H2O -> L_Glutamate + Diphosphate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant D127A]

L_Glutamine + 5_Phospho_alpha_D_ribose_1_diphosphate + H2O -> 5_Phospho_beta_D_ribosylamine + Diphosphate + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant N101G]

H2O + L_Glutamine + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + 5_Phospho_beta_D_ribosylamine + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant G102A]

H2O + 5_Phospho_alpha_D_ribose_1_diphosphate + L_Glutamine -> Diphosphate + L_Glutamate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant R73H]

H2O + 5_Phospho_alpha_D_ribose_1_diphosphate + L_Glutamine -> Diphosphate + 5_Phospho_beta_D_ribosylamine + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant R73L/Y74L]

NH3 + H2O + 5_Phospho_alpha_D_ribose_1_diphosphate -> 5_Phospho_beta_D_ribosylamine + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8.5
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant Y74F]

H2O + NH3 + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8.5
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant Y74L]

H2O + NH3 + 5_Phospho_alpha_D_ribose_1_diphosphate -> 5_Phospho_beta_D_ribosylamine + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8.5
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) wildtype]

H2O + 5_Phospho_alpha_D_ribose_1_diphosphate + L_Glutamine -> 5_Phospho_beta_D_ribosylamine + L_Glutamate + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant Y74F]

5_Phospho_alpha_D_ribose_1_diphosphate + L_Glutamine + H2O -> 5_Phospho_beta_D_ribosylamine + Diphosphate + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant Y74L]

L_Glutamine + H2O + 5_Phospho_alpha_D_ribose_1_diphosphate -> L_Glutamate + 5_Phospho_beta_D_ribosylamine + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant R73L]

L_Glutamine + H2O + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + L_Glutamate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant R73H]

H2O + L_Glutamine + 5_Phospho_alpha_D_ribose_1_diphosphate -> 5_Phospho_beta_D_ribosylamine + L_Glutamate + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant R73L/Y74L]

L_Glutamine + H2O + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + L_Glutamate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant Y74S]

H2O + L_Glutamine + 5_Phospho_alpha_D_ribose_1_diphosphate -> L_Glutamate + 5_Phospho_beta_D_ribosylamine + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant R73L/Y74L]

H2O + L_Glutamine -> NH3 + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant Y74S]

5_Phospho_alpha_D_ribose_1_diphosphate + NH3 + H2O -> Diphosphate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM ATP, 2 mM glycine, 10 mM MgOAc, glycineamide ribonucleotide synthetase
PH: 8.5
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant Y74F]

L_Glutamine + H2O + 5_Phospho_alpha_D_ribose_1_diphosphate -> 5_Phospho_beta_D_ribosylamine + Diphosphate + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl, 1 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) wildtype]

H2O + L_Glutamine -> L_Glutamate + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM alpha-ketoglutarate, 250 µM NADPH, 0.1 mM EDTA, glutamate dehydrogenase
PH: 7.6
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + H2O + L_Glutamine -> L_Glutamate + 5_Phospho_beta_D_ribosylamine + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mg/ml bovine serum albumin, 5.0 mM ATP, 2 mM glycine, 10 mM MgCl2, glycinamide ribonucleotide synthetase
PH: 7
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant L415A]

H2O + L_Glutamine + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + 5_Phospho_beta_D_ribosylamine + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mg/ml bovine serum albumin, 5.0 mM ATP, 2 mM glycine, 10 mM MgCl2, glycinamide ribonucleotide synthetase
PH: 7
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant L415A]

L_Glutamine + H2O -> NH3 + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM alpha-ketoglutarate, 250 µM NADPH, 0.1 mM EDTA, glutamate dehydrogenase
PH: 7.6
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) wildtype]

NH3 + 5_Phospho_alpha_D_ribose_1_diphosphate + H2O -> Diphosphate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mg/ml bovine serum albumin, 5.0 mM ATP, 2 mM glycine, 10 mM MgCl2, glycinamide ribonucleotide synthetase
PH: 7
Temperature: 37
uniprot:P0AG16 [amidophosphoribosyltransferase(Enzyme) mutant L415A]

NH3 + H2O + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mg/ml bovine serum albumin, 5.0 mM ATP, 2 mM glycine, 10 mM MgCl2, glycinamide ribonucleotide synthetase
PH: 7
Temperature: 37
uniprot:Q9STG9 [amidophosphoribosyltransferase(Enzyme) wildtype GPRAT2 (76-561)]

5_Phospho_alpha_D_ribose_1_diphosphate + L_Glutamine + H2O -> L_Glutamate + Diphosphate + 5_Phospho_beta_D_ribosylamine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 10 mM DTT
PH: 7.8
Temperature: 36

Pathways

pathway id name
BioCyc:HUMAN_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:META_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:ECO_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:YEAST_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:ARA_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:MTBCDC1551_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:MTBCDC1551_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:BSUB_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:ECOL316407_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:YEAST_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:MTBH37RV_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:SYNEL_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:SYNEL_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:ECOL316407_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:META_PWY-7282 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis (yeast)
BioCyc:ECO_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:ECO_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:ARA_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:BSUB_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:ECOL316407_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:HUMAN_PWY-841 purine nucleotides de novo biosynthesis
BioCyc:META_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:META_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:META_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:YEAST_THISYNARA-PWY-1 superpathway of thiamin diphosphate biosynthesis III (eukaryotes)
BioCyc:YEAST_PWY-7282 4-amino-2-methyl-5-phosphomethylpyrimidine biosynthesis
BioCyc:MTBH37RV_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:MTBH37RV_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:MTBCDC1551_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:MTBCDC1551_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:BSUB_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:BSUB_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:SYNEL_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:SYNEL_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:SYNEL_DENOVOPURINE2-PWY superpathway of purine nucleotides de novo biosynthesis II
PathBank:SMP0000927 Purine Nucleotides De Novo Biosynthesis
PathBank:SMP0002042 Thiamin Diphosphate Biosynthesis
PathBank:SMP0002047 Purine Nucleotides De Novo Biosynthesis 2
PlantCyc:PLANT_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
PlantCyc:PLANT_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
PlantCyc:ARA_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
PlantCyc:ARA_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
PlantCyc:ARA_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
Reactome:R-GGA-1660598 Metabolism
Reactome:R-HSA-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-RNO-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-GGA-419470 Nucleotide metabolism
Reactome:R-GGA-419140 De novo synthesis of IMP
Reactome:R-HSA-8956320 Nucleotide biosynthesis
Reactome:R-MMU-8956320 Nucleotide biosynthesis
Reactome:R-RNO-8956320 Nucleotide biosynthesis
Reactome:R-DME-15869 Nucleotide metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-DME-8956320 Nucleotide biosynthesis
Reactome:R-GGA-419474 Purine metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-RNO-15869 Nucleotide metabolism
WikiPathways:WP4224 Purine metabolism and related disorders
PathBank:SMP0120666 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120670 Adenylosuccinate Lyase Deficiency
PathBank:SMP0120714 Homocarnosinosis
PathBank:SMP0120850 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120448 Adenosine Deaminase Deficiency
PathBank:SMP0120452 AICA-Ribosiduria
PathBank:SMP0120496 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0120508 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0120532 Molybdenum Cofactor Deficiency
PathBank:SMP0120536 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0120577 Xanthinuria Type I
PathBank:SMP0120601 Mitochondrial DNA Depletion Syndrome
PathBank:SMP0000339 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0000609 Mercaptopurine Metabolism Pathway
PathBank:SMP0087278 Glutamate Metabolism
PathBank:SMP0063668 Purine Metabolism
PathBank:SMP0120669 Adenosine Deaminase Deficiency
PathBank:SMP0120673 AICA-Ribosiduria
PathBank:SMP0120709 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0120717 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0120797 Xanthinuria Type II
PathBank:SMP0120821 Myoadenylate Deaminase Deficiency
PathBank:SMP0120600 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000385 Homocarnosinosis
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000136 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000567 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120716 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0120728 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0120752 Molybdenum Cofactor Deficiency
PathBank:SMP0120756 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0120796 Xanthinuria Type I
PathBank:SMP0120820 Mitochondrial DNA Depletion Syndrome
PathBank:SMP0120445 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120449 Adenylosuccinate Lyase Deficiency
PathBank:SMP0120489 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0120497 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0120578 Xanthinuria Type II
PathBank:SMP0120602 Myoadenylate Deaminase Deficiency
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0087332 Purine Metabolism
PathBank:SMP0063590 Glutamate Metabolism
PathBank:SMP0120659 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120819 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0120438 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120494 Homocarnosinosis
PathBank:SMP0120631 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000072 Glutamate Metabolism
PathBank:SMP0000243 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000427 Azathioprine Action Pathway