EC: 1.16.3.1

ferroxidase (Fe(II):oxygen oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P07798 [ferroxidase(Enzyme) wildtype M(H')]

O2 + 4 Fe2+ + 4 H+ -> 2 H2O + 4 Fe3+		 (( (E * kcat) ) / (1 + (Kapp / (A ^ n)))) buffer: 200 mM Mops
PH: 7
Temperature: 36
uniprot:P07229 [ferroxidase(Enzyme) wildtype H]

4 H+ + O2 + 4 Fe2+ -> 4 Fe3+ + 2 H2O		 (( (E * kcat) ) / (1 + (Kapp / (A ^ n)))) buffer: 200 mM Mops
PH: 7
Temperature: 36
uniprot:P07797 [ferroxidase(Enzyme) mutant L+4]

O2 + 4 H+ + 4 Fe2+ -> 2 H2O + 4 Fe3+		 (( (E * kcat) ) / (1 + (Kapp / (A ^ n)))) buffer: 200 mM Mops
PH: 7
Temperature: 36
uniprot:P07797 [ferroxidase(Enzyme) mutant L+3]

4 H+ + 4 Fe2+ + O2 -> 2 H2O + 4 Fe3+		 (( (E * kcat) ) / (1 + (Kapp / (A ^ n)))) buffer: 200 mM Mops
PH: 7
Temperature: 36
- [Cu+]

4 H+ + O2 + 4 Fe2+ -> 2 H2O + 4 Fe3+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 0.1 M MES, 5 % Acetonitrile
PH: 5
Temperature: 36
uniprot:P00450 [Cu+]

4 H+ + O2 + 4 Fe2+ -> 2 H2O + 4 Fe3+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 0.1 M MES, 5 % Acetonitrile
PH: 5
Temperature: 36
- [Fe2+]

O2 + 4 H+ + 4 Cu+ -> 2 H2O + 4 Cu2+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 0.1 M MES, 5 % Acetonitrile
PH: 5
Temperature: 36
uniprot:P00450 [Fe2+]

O2 + 4 H+ + 4 Cu+ -> 2 H2O + 4 Cu2+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 0.1 M MES, 5 % Acetonitrile
PH: 5
Temperature: 36
uniprot:P38993 [ferroxidase(Enzyme) wildtype deglycosylated]

O2 + 4 Fe2+ + 4 H+ -> 2 H2O + 4 Fe3+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M MES
PH: 6
Temperature: 25
uniprot:P38993 [ferroxidase(Enzyme) wildtype deglycosylated]

4 H+ + O2 + 4 Fe2+ -> 2 H2O + 4 Fe3+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M MES
PH: 6
Temperature: 25
uniprot:P38993 [ferroxidase(Enzyme) wildtype glycosylated]

4 H+ + 4 Fe2+ + O2 -> 2 H2O + 4 Fe3+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M MES
PH: 6
Temperature: 25
uniprot:P38993 [ferroxidase(Enzyme) wildtype glycosylated]

O2 + 4 Fe2+ + 4 H+ -> 2 H2O + 4 Fe3+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M MES
PH: 6
Temperature: 25
uniprot:P38993 [ferroxidase(Enzyme) wildtype partially loaded]

4 Fe2+ + 4 H+ + O2 -> 2 H2O + 4 Fe3+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Sodium acetate, 100 µM Citric acid
PH: 5
Temperature: 22
uniprot:P38993 [ferroxidase(Enzyme) wildtype fully loaded]

4 Fe2+ + 4 H+ + O2 -> 4 Fe3+ + 2 H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Sodium acetate, 100 µM Citric acid
PH: 5
Temperature: 22
- [ferroxidase(Enzyme) wildtype]

O2 + 4 H+ + 4 Fe2+ -> 4 Fe3+ + 2 H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM MES
PH: 5
Temperature: 40
uniprot:G2H480 [Cu2+]

4 Fe2+ + 4 H+ + O2 -> 2 H2O + 4 Fe3+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Na-acetate
PH: 5.1
Temperature: 30
uniprot:O67206 [Cu2+]

O2 + 4 Fe2+ + 4 H+ -> 2 H2O + 4 Fe3+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Mes
PH: 5
Temperature: 40
uniprot:O67206 [ferroxidase(Enzyme) mutant deltaP321-V363]

4 Fe2+ + O2 + 4 H+ -> 2 H2O + 4 Fe3+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Mes
PH: 5
Temperature: 40
uniprot:O67206 [Cu2+]

O2 + 4 H+ + 4 Fe2+ -> 2 H2O + 4 Fe3+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Mes
PH: 5
Temperature: 40
uniprot:O67206 [ferroxidase(Enzyme) wildtype]

4 Fe2+ + O2 + 4 H+ -> 4 Fe3+ + 2 H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Mes
PH: 5
Temperature: 40
uniprot:A0A0H3PBA4 [ferroxidase(Enzyme) wildtype]

O2 + 4 H+ + 4 Fe2+ -> 2 H2O + 4 Fe3+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM MES
PH: 5
Temperature: 37
- [ferroxidase(Enzyme) mutant 1D11 (K245R-I441L-M449T-R465G-P58S-Y172C-I199T) -F55S]

4 H+ + 4 Fe2+ + O2 -> 4 Fe3+ + 2 H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM MES
PH: 5
Temperature: 40
- [ferroxidase(Enzyme) mutant 1D11 (K245R-I441L-M449T-R465G-P58S-Y172C-I199T)]

O2 + 4 Fe2+ + 4 H+ -> 4 Fe3+ + 2 H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM MES
PH: 5
Temperature: 40
- [ferroxidase(Enzyme) mutant 1D11 (K245R-I441L-M449T-R465G-P58S-Y172C-I199T) -V19A]

4 H+ + 4 Fe2+ + O2 -> 4 Fe3+ + 2 H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM MES
PH: 5
Temperature: 40
- [ferroxidase(Enzyme) mutant 1D11 (K245R-I441L-M449T-R465G-P58S-Y172C-I199T) -F17S]

4 H+ + O2 + 4 Fe2+ -> 4 Fe3+ + 2 H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM MES
PH: 5
Temperature: 40
- [ferroxidase(Enzyme) mutant 2B3 (F55S-F17S-V19A-P58S-Y172C-I199T-R465G-K245R-I441L-M449T)]

4 Fe2+ + 4 H+ + O2 -> 4 Fe3+ + 2 H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM MES
PH: 5
Temperature: 40
- [ferroxidase(Enzyme) wildtype]

4 Fe2+ + 4 H+ + O2 -> 2 H2O + 4 Fe3+		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM MES
PH: 5
Temperature: 40
- [ferroxidase(Enzyme) mutant 2G6 (K245R-I441L-M449T-R465G) -P58S]

4 H+ + 4 Fe2+ + O2 -> 4 Fe3+ + 2 H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM MES
PH: 5
Temperature: 40
- [ferroxidase(Enzyme) mutant 2G6 (K245R-I441L-M449T-R465G) -Y172C]

O2 + 4 H+ + 4 Fe2+ -> 4 Fe3+ + 2 H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM MES
PH: 5
Temperature: 40
- [ferroxidase(Enzyme) mutant 2G6 (K245R-I441L-M449T-R465G)]

4 H+ + 4 Fe2+ + O2 -> 2 H2O + 4 Fe3+		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM MES
PH: 5
Temperature: 40
- [ferroxidase(Enzyme) mutant 2G6 (K245R-I441L-M449T-R465G) -I199T-Y172C]

O2 + 4 H+ + 4 Fe2+ -> 4 Fe3+ + 2 H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM MES
PH: 5
Temperature: 40
- [ferroxidase(Enzyme) mutant 1B7 (K245R-I441L-M449T)]

4 H+ + 4 Fe2+ + O2 -> 2 H2O + 4 Fe3+		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM MES
PH: 5
Temperature: 40
- [ferroxidase(Enzyme) mutant 2G6 (K245R-I441L-M449T-R465G) -I199T]

O2 + 4 Fe2+ + 4 H+ -> 4 Fe3+ + 2 H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM MES
PH: 5
Temperature: 40
- [ferroxidase(Enzyme) mutant 2G6 (K245R-I441L-M449T-R465G) -I199T-P58S]

4 Fe2+ + 4 H+ + O2 -> 4 Fe3+ + 2 H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM MES
PH: 5
Temperature: 40

Pathways

pathway id name
BioCyc:YEAST_PWY-7250 iron-sulfur cluster biosynthesis
BioCyc:META_PWY-7250 [2Fe-2S] iron-sulfur cluster biosynthesis
Reactome:R-HSA-9635486 Infection with Mycobacterium tuberculosis
Reactome:R-HSA-9824439 Bacterial Infection Pathways
Reactome:R-DDI-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-MMU-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-RNO-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-BTA-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-CEL-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-DME-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-HSA-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-SCE-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-SPO-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-MMU-9865881 Complex III assembly
Reactome:R-RNO-9865881 Complex III assembly
Reactome:R-BTA-9865881 Complex III assembly
Reactome:R-DDI-9865881 Complex III assembly
Reactome:R-DME-9865881 Complex III assembly
Reactome:R-HSA-9865881 Complex III assembly
Reactome:R-SCE-9865881 Complex III assembly
Reactome:R-SPO-9865881 Complex III assembly
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-BTA-71403 Citric acid cycle (TCA cycle)
Reactome:R-BTA-1362409 Mitochondrial iron-sulfur cluster biogenesis
Reactome:R-DME-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-DME-71403 Citric acid cycle (TCA cycle)
Reactome:R-GGA-5653656 Vesicle-mediated transport
Reactome:R-GGA-199992 trans-Golgi Network Vesicle Budding
Reactome:R-HSA-425410 Metal ion SLC transporters
Reactome:R-HSA-611105 Respiratory electron transport
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-5619102 SLC transporter disorders
Reactome:R-HSA-5619049 Defective SLC40A1 causes hemochromatosis 4 (HFE4) (macrophages)
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-611105 Respiratory electron transport
Reactome:R-MMU-425410 Metal ion SLC transporters
Reactome:R-RNO-611105 Respiratory electron transport
Reactome:R-RNO-1362409 Mitochondrial iron-sulfur cluster biogenesis
Reactome:R-RNO-382551 Transport of small molecules
Reactome:R-RNO-425407 SLC-mediated transmembrane transport
Reactome:R-RNO-917937 Iron uptake and transport
Reactome:R-SCE-611105 Respiratory electron transport
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-SPO-71403 Citric acid cycle (TCA cycle)
Reactome:R-BTA-611105 Respiratory electron transport
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-611105 Respiratory electron transport
Reactome:R-HSA-5619115 Disorders of transmembrane transporters
Reactome:R-HSA-1222499 Latent infection - Other responses of Mtb to phagocytosis
Reactome:R-HSA-1222449 Mtb iron assimilation by chelation
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SPO-611105 Respiratory electron transport
Reactome:R-CEL-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-CEL-71403 Citric acid cycle (TCA cycle)
Reactome:R-CEL-1362409 Mitochondrial iron-sulfur cluster biogenesis
Reactome:R-DDI-611105 Respiratory electron transport
Reactome:R-DME-1362409 Mitochondrial iron-sulfur cluster biogenesis
Reactome:R-GGA-382551 Transport of small molecules
Reactome:R-GGA-917937 Iron uptake and transport
Reactome:R-GGA-199991 Membrane Trafficking
Reactome:R-HSA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-HSA-71403 Citric acid cycle (TCA cycle)
Reactome:R-HSA-1362409 Mitochondrial iron-sulfur cluster biogenesis
Reactome:R-HSA-5655799 Defective SLC40A1 causes hemochromatosis 4 (HFE4) (duodenum)
Reactome:R-MMU-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-MMU-71403 Citric acid cycle (TCA cycle)
Reactome:R-MMU-1362409 Mitochondrial iron-sulfur cluster biogenesis
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-RNO-71403 Citric acid cycle (TCA cycle)
Reactome:R-SCE-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-SCE-71403 Citric acid cycle (TCA cycle)
Reactome:R-SPO-1362409 Mitochondrial iron-sulfur cluster biogenesis
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-DDI-71403 Citric acid cycle (TCA cycle)
Reactome:R-DDI-1362409 Mitochondrial iron-sulfur cluster biogenesis
Reactome:R-GGA-432722 Golgi Associated Vesicle Biogenesis
Reactome:R-HSA-382551 Transport of small molecules
Reactome:R-HSA-425407 SLC-mediated transmembrane transport
Reactome:R-HSA-917937 Iron uptake and transport
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-MMU-382551 Transport of small molecules
Reactome:R-MMU-425407 SLC-mediated transmembrane transport
Reactome:R-MMU-917937 Iron uptake and transport
Reactome:R-RNO-425410 Metal ion SLC transporters
Reactome:R-SCE-1362409 Mitochondrial iron-sulfur cluster biogenesis
PathBank:SMP0119394 Coagulation
PathBank:SMP0000024 Porphyrin Metabolism
PathBank:SMP0120689 Congenital Erythropoietic Porphyria (CEP) or Gunther Disease
PathBank:SMP0000345 Congenital Erythropoietic Porphyria (CEP) or Gunther Disease
PathBank:SMP0120668 Acute Intermittent Porphyria
PathBank:SMP0120712 Hereditary Coproporphyria (HCP)
PathBank:SMP0120764 Porphyria Variegata (PV)
PathBank:SMP0000344 Acute Intermittent Porphyria
PathBank:SMP0087336 Porphyrin Metabolism
PathBank:SMP0000346 Porphyria Variegata (PV)
PathBank:SMP0000342 Hereditary Coproporphyria (HCP)
PathBank:SMP0087243 Porphyrin Metabolism