Raffinose
Raffinose is a complex carbohydrate. It is a trisaccharide composed of galactose, fructose, and glucose. It can be found in beans, cabbage, brussels sprouts, broccoli, asparagus, other vegetables, and whole grains. Raffinose is hydrolyzed to D-galactose and sucrose by D-galactosidase (D-GAL). D-GAL also hydrolyzes other D-galactosides such as stachyose, verbascose, and galactinol [1-O-(D-galactosyl)-myoinositol], if present. The enzyme does not cleave linked galactose, as in lactose. Raffinose is also known as melitose and may be thought of as galactose and sucrose connected via an alpha(1->6) glycosidic linkage. Thus, raffinose can be broken down into galactose and sucrose via the enzyme alpha-galactosidase. Human intestines do not contain this enzyme. Raffinose is a trisaccharide occurring in Australian manna (from Eucalyptus spp, Myrtaceae) and in cottonseed meal.
| ID | EC Number | Name |
|---|---|---|
| KEGG:R00039 | 2.4.1.166 | raffinose:raffinose 1F-alpha-D-galactosyltransferase |
| KEGG:R01103 | 3.2.1.22 | raffinose galactohydrolase |
| KEGG:R02410 | 3.2.1.26 | raffinose fructohydrolase |
| KEGG:R02411 | 2.4.1.82 | 1-alpha-D-galactosyl-myo-inositol:sucrose 6-alpha-D-galactosyltransferase |
| KEGG:R03418 | 2.4.1.67 | 1-alpha-D-galactosyl-myo-inositol:raffinose galactosyltransferase |
| KEGG:R03634 | 3.2.1.22 | C01613 + C00001<=>C00492 + C00124 |
| KEGG:R08616 | 2.4.1.166 | raffinose:raffinose 3F-alpha-D-galactosyltransferase |
| BioCyc:RXN-11502 | 3.2.1.- | CPD-1099 + WATER --> ALPHA-D-GALACTOSE + SUCROSE |
| BioCyc:RXN-11501 | 3.2.1.22 | CPD-170 + WATER --> CPD-1099 + ALPHA-D-GALACTOSE |
| Rhea:RHEA:14126 | 2.4.1.166 | 2 raffinose => 1F-α-D-galactosylraffinose + sucrose |
| Rhea:RHEA:14127 | 2.4.1.166 | 1F-α-D-galactosylraffinose + sucrose => 2 raffinose |
| Rhea:RHEA:14128 | 2.4.1.166 | 2 raffinose <=> 1F-α-D-galactosylraffinose + sucrose |
| Rhea:RHEA:20162 | 2.4.1.82 | α-D-galactosyl-(1→3)-1D-myo-inositol + sucrose => raffinose + myo-inositol |
| Rhea:RHEA:20163 | 2.4.1.82 | raffinose + myo-inositol => α-D-galactosyl-(1→3)-1D-myo-inositol + sucrose |
| Rhea:RHEA:20164 | 2.4.1.82 | α-D-galactosyl-(1→3)-1D-myo-inositol + sucrose <=> raffinose + myo-inositol |
| Rhea:RHEA:20777 | 2.4.1.67 | α-D-galactosyl-(1→3)-1D-myo-inositol + raffinose => stachyose + myo-inositol |
| Rhea:RHEA:20778 | 2.4.1.67 | stachyose + myo-inositol => α-D-galactosyl-(1→3)-1D-myo-inositol + raffinose |
| Rhea:RHEA:20779 | 2.4.1.67 | α-D-galactosyl-(1→3)-1D-myo-inositol + raffinose <=> stachyose + myo-inositol |
| Rhea:RHEA:70276 | raffinose + H2O => sucrose + D-galactose | |
| Rhea:RHEA:70277 | sucrose + D-galactose => raffinose + H2O | |
| Rhea:RHEA:70278 | raffinose + H2O <=> sucrose + D-galactose | |
| Rhea:RHEA:70280 | stachyose + H2O => raffinose + D-galactose | |
| Rhea:RHEA:70281 | raffinose + D-galactose => stachyose + H2O | |
| Rhea:RHEA:70282 | stachyose + H2O <=> raffinose + D-galactose | |
| BioCyc:2.4.1.67-RXN | 2.4.1.67 | CPD-458 + CPD-1099<=>MYO-INOSITOL + CPD-170 |
| BioCyc:RXN-11492 | 2.4.1.- | 2 CPD-1099 --> CPD-12464 + SUCROSE |
| BioCyc:RXN-8287 | 2.4.1.- | 2 CPD-1099 --> CPD-170 + SUCROSE |
| BioCyc:RXN-11490 | 2.4.1.166 | 2 CPD-1099 --> CPD-12462 + SUCROSE |
| BioCyc:RXN-15224 | 2.4.1.166 | 2 CPD-1099 --> CPD-16418 + SUCROSE |
| BioCyc:2.4.1.82-RXN | 2.4.1.82 | CPD-458 + SUCROSE --> MYO-INOSITOL + CPD-1099 |