CYP82D61

Short Conclusion Abstract: CYP82D61 (EC 1.14.14.132) is a cytochrome P450 monooxygenase that catalyzes the 4-hydroxylation of (-)-4'-demethyl-deoxypodophyllotoxin, forming podophyllotoxin—a critical precursor for clinically used antitumor agents (e.g., etoposide). This enzymatic step is essential in the biosynthetic pathway of podophyllotoxin in Podophyllum spp., directly enabling the production of pharmacologically active lignans. Without CYP82D61-mediated hydroxylation at the C4 position, the pathway cannot generate the structural motif required for the anticancer activity of downstream derivatives. Thus, CYP82D61 is a key committed enzyme in the synthesis of plant-derived chemotherapeutic compounds.

Protein Function: (-)-4'-demethyl-deoxypodophyllotoxin 4-hydroxylase [EC:1.14.14.132]

DBLinks
Proteins
protein id name function taxonomy source
Reactions
reaction id name note
KEGG:R11212 (-)-deoxypodophyllotoxin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (4-hydroxylating) 4'-Demethyldeoxypodophyllotoxin + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> 4'-Demethylepipodophyllotoxin + [Oxidized NADPH---hemoprotein reductase] + H2O
Rhea:RHEA:49033 (−)-4'-desmethyl-deoxypodophyllotoxin + reduced [NADPH—hemoprotein reductase] + O2 => 4'-demethylepipodophyllotoxin + oxidized [NADPH—hemoprotein reductase] + H2O + H+
Rhea:RHEA:49034 4'-demethylepipodophyllotoxin + oxidized [NADPH—hemoprotein reductase] + H2O + H+ => (−)-4'-desmethyl-deoxypodophyllotoxin + reduced [NADPH—hemoprotein reductase] + O2
Rhea:RHEA:49035 (−)-4'-desmethyl-deoxypodophyllotoxin + reduced [NADPH—hemoprotein reductase] + O2 <=> 4'-demethylepipodophyllotoxin + oxidized [NADPH—hemoprotein reductase] + H2O + H+
BioCyc:RXN-17350 CPD-18756 + OXYGEN-MOLECULE + Red-NADPH-Hemoprotein-Reductases --> CPD-18757 + WATER + Ox-NADPH-Hemoprotein-Reductases
BioCyc:RXN-8737 CPD-8958 + OXYGEN-MOLECULE + Red-NADPH-Hemoprotein-Reductases --> CPD-18758 + WATER + Ox-NADPH-Hemoprotein-Reductases