3-methylbutanol biosynthesis (engineered)
Connor MR, Liao JC. Engineering of an Escherichia coli Strain for the Production of 3-Methyl-1-Butanol. Appl Environ Microbiol. 2008 Sep 15;74(18):5769–75. doi: 10.1128/aem.00468-08.; Dickinson JR, Lanterman MM, Danner DJ, Pearson BM, Sanz P, Harrison SJ, Hewlins MJE. A 13C Nuclear Magnetic Resonance Investigation of the Metabolism of Leucine to Isoamyl Alcohol in Saccharomyces cerevisiae. Journal of Biological Chemistry. 1997 Oct;272(43):26871–8. doi: 10.1074/jbc.272.43.26871.
Metabolites
Enzyme
| EC Number | name | full name | note |
|---|---|---|---|
| 1.1.1.- | |||
| 1.1.1.1 | alcohol dehydrogenase | alcohol:NAD+ oxidoreductase | |
| 1.1.1.284 | S-(hydroxymethyl)glutathione dehydrogenase | S-(hydroxymethyl)glutathione:NAD+ oxidoreductase | |
| 1.1.1.54 | allyl-alcohol dehydrogenase | allyl-alcohol:NADP+ oxidoreductase | |
| 1.1.1.78 | methylglyoxal reductase (NADH) | (R)-lactaldehyde:NAD+ oxidoreductase | |
| 2.3.3.13 | 2-isopropylmalate synthase | acetyl-CoA:3-methyl-2-oxobutanoate C-acetyltransferase (thioester-hydrolysing, carboxymethyl-forming) | |
| 4.1.1.72 | branched-chain-2-oxoacid decarboxylase | (3S)-3-methyl-2-oxopentanoate carboxy-lyase (2-methylbutanal-forming) |
Proteins
| Protein ID | name | full name |
|---|---|---|
| P00330 | ADH1 | Alcohol dehydrogenase 1 |
| P00331 | ADH2 | Alcohol dehydrogenase 2 |
| P07246 | ADH3 | Alcohol dehydrogenase 3, mitochondrial |
| P09151 | leuA | 2-isopropylmalate synthase |
| P10127 | ADH4 | Alcohol dehydrogenase 4 |
| P32771 | SFA1 | S-(hydroxymethyl)glutathione dehydrogenase |
| P38113 | ADH5 | Alcohol dehydrogenase 5 |
| Q07471 | THI3 | Thiamine metabolism regulatory protein THI3 |