EC: 6.4.1.3

propionyl-CoA carboxylase (propanoyl-CoA:carbon-dioxide ligase (ADP-forming))

enzyme lambda metabolic reaction experiment
- [propionyl-CoA carboxylase(Enzyme) wildtype]

Hydrogencarbonate + Propanoyl_CoA + ATP -> Phosphate + (S)_2_Methyl_3_oxopropanoyl_CoA + ADP		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 5 mM glutathione, 100 mM KCl, 10 mM MgCl2
PH: 8
Temperature: 37
- [propionyl-CoA carboxylase(Enzyme) wildtype]

Hydrogencarbonate + ATP + Propanoyl_CoA -> ADP + Phosphate + (S)_2_Methyl_3_oxopropanoyl_CoA		 (( (Vmax * C) ) / (Km + C)) buffer: 50 mM Tris-HCl, 5 mM glutathione, 100 mM KCl, 10 mM MgCl2
PH: 8
Temperature: 37
- [propionyl-CoA carboxylase(Enzyme) wildtype]

Hydrogencarbonate + ATP + Propanoyl_CoA -> ADP + Phosphate + (S)_2_Methyl_3_oxopropanoyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5 mM glutathione, 100 mM KCl, 10 mM MgCl2
PH: 8
Temperature: 37
- [propionyl-CoA carboxylase(Enzyme) wildtype]

Hydrogencarbonate + Butanoyl_CoA + ATP -> ADP + (S)_2_Ethyl_3_oxopropanoyl_CoA + Phosphate		 (( (Vmax * C) ) / (Km + C)) buffer: 50 mM Tris-HCl, 5 mM glutathione, 100 mM KCl, 10 mM MgCl2
PH: 8
Temperature: 37
- [propionyl-CoA carboxylase(Enzyme) mutant E168K]

Hydrogencarbonate + Propanoyl_CoA + ATP -> Phosphate + ADP + (S)_2_Methyl_3_oxopropanoyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris, 0.5 mg/ml bovine serum albumin, 125 mM KCl, 10 mM MgCl2
PH: 8
Temperature: 37
- [propionyl-CoA carboxylase(Enzyme) mutant R410W]

Hydrogencarbonate + Propanoyl_CoA + ATP -> (S)_2_Methyl_3_oxopropanoyl_CoA + Phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris, 0.5 mg/ml bovine serum albumin, 125 mM KCl, 10 mM MgCl2
PH: 8
Temperature: 37
- [propionyl-CoA carboxylase(Enzyme) mutant R165W]

Hydrogencarbonate + Propanoyl_CoA + ATP -> ADP + Phosphate + (S)_2_Methyl_3_oxopropanoyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris, 0.5 mg/ml bovine serum albumin, 125 mM KCl, 10 mM MgCl2
PH: 8
Temperature: 37
- [propionyl-CoA carboxylase(Enzyme) mutant A497V]

Hydrogencarbonate + ATP + Propanoyl_CoA -> Phosphate + ADP + (S)_2_Methyl_3_oxopropanoyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris, 0.5 mg/ml bovine serum albumin, 125 mM KCl, 10 mM MgCl2
PH: 8
Temperature: 37
- [propionyl-CoA carboxylase(Enzyme) wildtype]

Propanoyl_CoA + Hydrogencarbonate + ATP -> (S)_2_Methyl_3_oxopropanoyl_CoA + ADP + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris, 0.5 mg/ml bovine serum albumin, 125 mM KCl, 10 mM MgCl2
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-5328 superpathway of methionine degradation
BioCyc:META_PWY-5328 superpathway of L-methionine salvage and degradation
BioCyc:BSUB_PROPIONMET-PWY propanoyl CoA degradation I
BioCyc:META_PWY-5130 2-oxobutanoate degradation I
BioCyc:MOUSE_PWY-5328 superpathway of methionine degradation
BioCyc:MOUSE_PWY3DJ-0 isoleucine degradation
BioCyc:HUMAN_PROPIONMET-PWY propionyl-CoA degradation
BioCyc:MOUSE_PROPIONMET-PWY methylmalonyl pathway
BioCyc:BSUB_PWY-5130 2-oxobutanoate degradation I
BioCyc:HUMAN_PWY-5130 2-oxobutanoate degradation
BioCyc:META_PROPIONMET-PWY propanoyl CoA degradation I
BioCyc:MOUSE_PWY3DJ-86 valine degradation
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-196780 Biotin transport and metabolism
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-MMU-196780 Biotin transport and metabolism
Reactome:R-RNO-8978868 Fatty acid metabolism
Reactome:R-RNO-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-RNO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-196780 Biotin transport and metabolism
Reactome:R-CEL-556833 Metabolism of lipids
Reactome:R-CEL-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-CEL-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-CEL-196780 Biotin transport and metabolism
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-HSA-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-3296482 Defects in vitamin and cofactor metabolism
Reactome:R-HSA-3371599 Defective HLCS causes multiple carboxylase deficiency
Reactome:R-MMU-8978868 Fatty acid metabolism
Reactome:R-MMU-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-CEL-8978868 Fatty acid metabolism
Reactome:R-CEL-71032 Propionyl-CoA catabolism
Reactome:R-HSA-71032 Propionyl-CoA catabolism
Reactome:R-HSA-5668914 Diseases of metabolism
Reactome:R-MMU-71032 Propionyl-CoA catabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-3323169 Defects in biotin (Btn) metabolism
Reactome:R-RNO-71032 Propionyl-CoA catabolism
WikiPathways:WP5031 Biotin metabolism, including IMDs
WikiPathways:WP4686 Leucine, isoleucine and valine metabolism
PathBank:SMP0120662 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120734 Malonic Aciduria
PathBank:SMP0120786 Malonyl-CoA Decarboxylase Deficiency
PathBank:SMP0120806 3-Hydroxyisobutyric Aciduria
PathBank:SMP0120440 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120444 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120516 Maple Syrup Urine Disease
PathBank:SMP0120528 Methylmalonic Aciduria Due to Cobalamin-Related Disorders
PathBank:SMP0120589 Isovaleric Acidemia
PathBank:SMP0000032 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0000016 Propanoate Metabolism
PathBank:SMP0000173 beta-Ketothiolase Deficiency
PathBank:SMP0000199 Maple Syrup Urine Disease
PathBank:SMP0000384 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000141 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0000524 Isovaleric Acidemia
PathBank:SMP0087234 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0087262 Threonine and 2-Oxobutanoate Degradation
PathBank:SMP0063656 Propanoate Metabolism
PathBank:SMP0120661 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120665 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120745 Methylmalonic Aciduria
PathBank:SMP0120757 Propionic Acidemia
PathBank:SMP0120805 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0120439 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120443 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120459 beta-Ketothiolase Deficiency
PathBank:SMP0120503 Isovaleric Aciduria
PathBank:SMP0120523 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120588 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000138 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0000140 3-Methylglutaconic Aciduria Type III
PathBank:SMP0000238 Isovaleric Aciduria
PathBank:SMP0000201 Methylmalonic Aciduria Due to Cobalamin-Related Disorders
PathBank:SMP0000523 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0087341 Propanoate Metabolism
PathBank:SMP0087449 Pyruvate Metabolism
PathBank:SMP0063678 Threonine and 2-Oxobutanoate Degradation
PathBank:SMP0063689 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0120660 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120664 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120736 Maple Syrup Urine Disease
PathBank:SMP0120748 Methylmalonic Aciduria Due to Cobalamin-Related Disorders
PathBank:SMP0120808 Isovaleric Acidemia
PathBank:SMP0120441 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120525 Methylmalonic Aciduria
PathBank:SMP0120537 Propionic Acidemia
PathBank:SMP0120586 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0000137 2-Methyl-3-hydroxybutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000237 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0000200 Methylmalonic Aciduria
PathBank:SMP0000521 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0087248 Propanoate Metabolism
PathBank:SMP0087480 Propanoate Metabolism
PathBank:SMP0087516 Threonine and 2-Oxobutanoate Degradation
PathBank:SMP0120663 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120679 beta-Ketothiolase Deficiency
PathBank:SMP0120723 Isovaleric Aciduria
PathBank:SMP0120743 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120807 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120442 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120514 Malonic Aciduria
PathBank:SMP0120567 Malonyl-CoA Decarboxylase Deficiency
PathBank:SMP0120587 3-Hydroxyisobutyric Aciduria
PathBank:SMP0000452 Threonine and 2-Oxobutanoate Degradation
PathBank:SMP0000236 Propionic Acidemia
PathBank:SMP0000139 3-Methylglutaconic Aciduria Type I
PathBank:SMP0000198 Malonic Aciduria
PathBank:SMP0000502 Malonyl-CoA Decarboxylase Deficiency
PathBank:SMP0000522 3-Hydroxyisobutyric Aciduria
PathBank:SMP0087327 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0087355 Threonine and 2-Oxobutanoate Degradation
PathBank:SMP0087499 Fatty Acid Biosynthesis