EC: 5.4.2.12

phosphoglycerate mutase (2,3-diphosphoglycerate-independent) (D-phosphoglycerate 2,3-phosphomutase (2,3-diphosphoglycerate-independent))

enzyme lambda metabolic reaction experiment
uniprot:P39773 [Mn2+]

3_Phospho_D_glycerate -> 2_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 2 mM MgCl2, 1 mM MnCl2, 1 mM ATP, 0.2 mM NADH, 2 U/ml of glyceraldehyde-3-phosphate dehydrogenase, 10 U/ml of 3-phosphoglycerate kinase
PH: 7.6
Temperature: 23
uniprot:P39773 [Mn2+]

3_Phospho_D_glycerate -> 2_Phospho_D_glycerate		 (( (Vmax * M) ) / (Km + M)) buffer: 50 mM Tris, 1 mM ATP, 0.2 mM NADH, 2 U/ml of glyceraldehyde-3-phosphate dehydrogenase, 10 U/ml of 3-phosphoglycerate kinase
PH: 7.6
Temperature: 23
uniprot:P39773 [Mn2+]

3_Phospho_D_glycerate -> 2_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium N-tris (hydroxymethyl) methyl-2-aminomethanesulfonic acid (TES), 5 mM MgCl2, 50 mM KCl, 1 mM ADP, 0.15 mM NADH, 1.5 U/ml of enolase, 1.5 U/ml of pyruvate kinase, 2.0 U/ml of lactate dehydrogenase
PH: 7.6
Temperature: 23
uniprot:P39773 [Mn2+]

3_Phospho_D_glycerate -> 2_Phospho_D_glycerate		 (( (Vmax * M) ) / (Km + M)) buffer: 50 mM potassium N-tris (hydroxymethyl) methyl-2-aminomethanesulfonic acid (TES), 50 mM KCl, 1 mM MnCl2, 1 mM ADP, 0.15 mM NADH, 1.5 U/ml of enolase, 1.5 U/ml of pyruvate kinase, 2.0 U/ml of lactate dehydrogenase
PH: 7.6
Temperature: 23
- [phosphoglycerate mutase (2,3-diphosphoglycerate-independent)(Enzyme) wildtype]

3_Phospho_D_glycerate -> 2_Phospho_D_glycerate		 (( (( (( (( (Vpf * VPFvPGM) ) * p3gPF) ) / (Kp3gPFvPGM * Vpf)) ) * (1 - (p2gPF / (p3gPF * KeqPFvPGM)))) ) / (( (1 + (p2gPF / (Kp2gPFvPGM * Vpf))) ) + (p3gPF / (Kp3gPFvPGM * Vpf)))) buffer: *
PH: 7
Temperature: 36
uniprot:Q7XYD2 [phosphoglycerate mutase (2,3-diphosphoglycerate-independent)(Enzyme) wildtype]

3_Phospho_D_glycerate -> 2_Phospho_D_glycerate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM beta,beta-dimethylglutarate-NaOH, 0.2 mM ADP, 20 mM KCl, 5 mM MgCl2, 0.2 mM NADH, 8.3 µg/ml Lactate dehydrogenase, 15 µg/ml Pyruvate kinase, 7.5 µg/ml Enolase
PH: 7
Temperature: 30
uniprot:Q9NG18 [phosphoglycerate mutase (2,3-diphosphoglycerate-independent)(Enzyme) wildtype]

3_Phospho_D_glycerate -> 2_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M triethanolamine-HCl, 1.0 mM MgSO4, 1.0 mM ADP, 0.25 mM NADH, 0.1 % triton X-100, 1 U/ml lactate dehydrogenase, 1 U/ml enolase
PH: 7.4
Temperature: 25
uniprot:Q9NG18 [phosphoglycerate mutase (2,3-diphosphoglycerate-independent)(Enzyme) wildtype]

2_Phospho_D_glycerate -> 3_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M triethanolamine-HCl, 5.0 mM MgSO4, 1.0 mM ATP, 0.42 mM NADH, 0.1 mM DTT, 4 U/ml glyceraldehyde-3-phosphate dehydrogenase
PH: 7.4
Temperature: 25
uniprot:Q4VWF8 [phosphoglycerate mutase (2,3-diphosphoglycerate-independent)(Enzyme) wildtype]

3_Phospho_D_glycerate -> 2_Phospho_D_glycerate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, 1 mM ADP, 0.15 mM NADH, 2.5 U/ml enolase, 2.5 U/ml pyruvate kinase, 2.5 U/ml L-lactic dehydrogenase
PH: 7
Temperature: 22
- [phosphoglycerate mutase (2,3-diphosphoglycerate-independent)(Enzyme) wildtype]

3_Phospho_D_glycerate -> 2_Phospho_D_glycerate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, 1 mM ADP, 0.15 mM NADH, 2.5 U/ml enolase, 2.5 U/ml pyruvate kinase, 2.5 U/ml L-lactic dehydrogenase
PH: 7
Temperature: 22
- [phosphoglycerate mutase (2,3-diphosphoglycerate-independent)(Enzyme) wildtype His6-tagged]

3_Phospho_D_glycerate -> 2_Phospho_D_glycerate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, 1 mM ADP, 0.15 mM NADH, 2.5 U/ml enolase, 2.5 U/ml pyruvate kinase, 2.5 U/ml L-lactic dehydrogenase
PH: 7
Temperature: 22
- [phosphoglycerate mutase (2,3-diphosphoglycerate-independent)(Enzyme) wildtype His-tagged]

Glycerate_3_phosphate -> 2_Phosphoglycerate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole, 10 mM Acetic acid/Mes/Tris, 5 mM MgCl2, 0.2 mM NADH, 0.9 mM ADP, 2.5 U enolase, 1 U pyruvate kinase/lactate dehydrogenase
PH: 7
Temperature: 37
- [phosphoglycerate mutase (2,3-diphosphoglycerate-independent)(Enzyme) wildtype His-tagged]

Glycerate_3_phosphate -> 2_Phosphoglycerate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole, 10 mM Acetic acid/Mes/Tris, 5 mM MgCl2, 0.2 mM NADH, 0.9 mM ADP, 2.5 U enolase, 1 U pyruvate kinase/lactate dehydrogenase
PH: 6
Temperature: 37
- [phosphoglycerate mutase (2,3-diphosphoglycerate-independent)(Enzyme) wildtype His-tagged]

2_Phosphoglycerate -> Glycerate_3_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole, 10 mM Acetic acid/Mes/Tris, 5 mM MgCl2, 1 mM EDTA, 2 mM dithiothreitol, 0.15 mM NADH, 0.16 U phosphoglycerate kinase, 1 mM GTP
PH: 6
Temperature: 37
- [phosphoglycerate mutase (2,3-diphosphoglycerate-independent)(Enzyme) wildtype His-tagged]

2_Phosphoglycerate -> Glycerate_3_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole, 10 mM Acetic acid/Mes/Tris, 5 mM MgCl2, 1 mM EDTA, 2 mM dithiothreitol, 0.15 mM NADH, 0.16 U phosphoglycerate kinase, 1 mM GTP
PH: 7
Temperature: 37
uniprot:O28523 [Mn2+]

3_Phospho_D_glycerate -> 2_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 mM Tris-HCl, 5 mM MgSO4,1 mM ADP, 0.3 mM NADH, 0.08 U enolase, 0.4 U pyruvate kinase, 0.5 U lactate dehydrogenase
PH: 7
Temperature: 50
uniprot:O28523 [Mn2+]

2_Phospho_D_glycerate -> 3_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 mM Tris-HCl, 5 mM MgSO4,1 mM ADP, 1 mM phosphoenolpyruvate, 0.3 mM NADH, glycerate kinase, 0.5 U lactate dehydrogenase, 0.4 U pyruvate kinase
PH: 7
Temperature: 50
uniprot:P37689 [phosphoglycerate mutase (2,3-diphosphoglycerate-independent)(Enzyme) wildtype]

3_Phospho_D_glycerate -> 2_Phospho_D_glycerate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 30 mM Tris-HCl, 20 mM KCl, 5 mM MgSO4, 1 mM ATP, 0.15 mM NADH, 0.08 U enolase, 0.5 U pyruvate kinase
PH: 7
Temperature: 30
uniprot:P37689 [phosphoglycerate mutase (2,3-diphosphoglycerate-independent)(Enzyme) wildtype]

2_Phospho_D_glycerate -> 3_Phospho_D_glycerate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 30 mM Tris-HCl, 20 mM KCl, 5 mM MgSO4, 0.2 mM ADP, 0.15 mM NADH, 0.5 U phosphoglycerate kinase, 0.5 U glyceraldehyde 3-phosphate dehydrogenase
PH: 7
Temperature: 30

Pathways

pathway id name
BioCyc:META_P441-PWY superpathway of N-acetylneuraminate degradation
BioCyc:META_ANAGLYCOLYSIS-PWY glycolysis III (from glucose)
BioCyc:ECO_GLUCONEO-PWY gluconeogenesis I
BioCyc:ARA_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:VCHO_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:HPY_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:SYNEL_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:SYNEL_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:ECOL316407_GLYCOLYSIS glycolysis I (from glucose-6P)
BioCyc:ECOL316407_GLYCOLYSIS-E-D superpathway of glycolysis and Entner-Doudoroff
BioCyc:ECOL316407_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:BTHE_PWY-6901 superpathway of glucose and xylose degradation
BioCyc:BTHE_GLYCOLYSIS glycolysis I (from glucose-6P)
BioCyc:BTHE_PWY-1042 glycolysis IV (plant cytosol)
BioCyc:BTHE_PWY66-400 glycolysis VI (metazoan)
BioCyc:META_PWY-5464 superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle
BioCyc:META_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:META_PWY-6142 gluconeogenesis II (Methanobacterium thermoautotrophicum)
BioCyc:META_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:META_PWY-1042 glycolysis IV (plant cytosol)
BioCyc:META_PWY-8004 Entner-Doudoroff pathway I
BioCyc:META_PWY-7345 superpathway of anaerobic sucrose degradation
BioCyc:ECO_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:ECO_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:ARA_PWY-1042 glycolysis IV (plant cytosol)
BioCyc:ARA_GLUCONEO-PWY gluconeogenesis I
BioCyc:ANTHRA_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:ANTHRA_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:ANTHRA_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:AGRO_PWY-6901 superpathway of glucose and xylose degradation
BioCyc:AGRO_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:BSUB_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:SYNEL_GLYCOLYSIS-E-D superpathway of glycolysis and Entner-Doudoroff
BioCyc:ECOL316407_GLUCONEO-PWY gluconeogenesis I
BioCyc:PDIF272563_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:META_PWY-7218 photosynthetic 3-hydroxybutanoate biosynthesis (engineered)
BioCyc:META_GLUCONEO-PWY gluconeogenesis I
BioCyc:META_PWY-6146 Methanobacterium thermoautotrophicum biosynthetic metabolism
BioCyc:ECO_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:ARA_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:ARA_PWY66-399 gluconeogenesis III
BioCyc:VCHO_P441-PWY superpathway of N-acetylneuraminate degradation
BioCyc:VCHO_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:HPY_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:ANTHRA_GLUCONEO-PWY gluconeogenesis I
BioCyc:AGRO_ANAGLYCOLYSIS-PWY glycolysis III (from glucose)
BioCyc:AGRO_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:AGRO_GLUCONEO-PWY gluconeogenesis I
BioCyc:BSUB_GLUCONEO-PWY-1 gluconeogenesis I
BioCyc:BSUB_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:ECOL316407_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:PDIF272563_GLUCONEO-PWY gluconeogenesis I
BioCyc:META_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:META_PWY-5723 Rubisco shunt
BioCyc:META_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:META_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:ECO_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:ECO_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:ARA_PWY-5723 Rubisco shunt
BioCyc:VCHO_GLUCONEO-PWY gluconeogenesis I
BioCyc:VCHO_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:VCHO_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:HPY_GLUCONEO-PWY gluconeogenesis I
BioCyc:AGRO_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:BSUB_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:SYNEL_PWY-5723 Rubisco shunt
BioCyc:SYNEL_GLUCONEO-PWY gluconeogenesis I
BioCyc:ECOL316407_PWY-5484 glycolysis II (from fructose-6P)
BioCyc:BTHE_GLUCONEO-PWY gluconeogenesis I
PathBank:SMP0000807 Glycolysis and Pyruvate Dehydrogenase
PathBank:SMP0000839 Gluconeogenesis from L-Malic Acid
PathBank:SMP0000930 Fructose Metabolism
PathBank:SMP0000931 Glycerol Metabolism
PathBank:SMP0000932 Glycerol Metabolism II
PathBank:SMP0000933 Glycerol Metabolism III (sn-Glycero-3-Phosphoethanolamine)
PathBank:SMP0000934 Glycerol Metabolism IV (Glycerophosphoglycerol)
PathBank:SMP0000935 Glycerol Metabolism V (Glycerophosphoserine)
PlantCyc:ARA_PWY-5464 superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle
PlantCyc:CORN_PWY-5723 Rubisco shunt
PlantCyc:CORN_GLUCONEO-PWY gluconeogenesis I
PlantCyc:ARA_PWY-7345 superpathway of anaerobic sucrose degradation
PlantCyc:PLANT_PWY-1042 glycolysis IV (plant cytosol)
PlantCyc:ARA_PWY-5723 Rubisco shunt
PlantCyc:ARA_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
PlantCyc:ARA_GLUCONEO-PWY gluconeogenesis I
PlantCyc:CORN_PWY-5464 superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle
PlantCyc:PLANT_PWY-5484 glycolysis II (from fructose 6-phosphate)
PlantCyc:ARA_PWY-5484 glycolysis II (from fructose 6-phosphate)
PlantCyc:ARA_PWY-1042 glycolysis IV (plant cytosol)
PlantCyc:CORN_PWY-1042 glycolysis IV
PlantCyc:CORN_PWY-5484 glycolysis II (from fructose 6-phosphate)
PlantCyc:CORN_PWY-7345 superpathway of anaerobic sucrose degradation
WikiPathways:WP2621 Glycolysis