EC: 5.1.1.1

alanine racemase (alanine racemase)

enzyme lambda metabolic reaction experiment
- [alanine racemase(Enzyme) wildtype]

L_Alanine -> D_Alanine		 (( (Vmax * A) ) / (Km + A)) buffer: 35 mM CHES-NaOH, 1.5 mM Diphenylamine
PH: 9
Temperature: 65
- [alanine racemase(Enzyme) wildtype]

L_Alanine -> D_Alanine		 (( (Vmax * A) ) / (Km + A)) buffer: 35 mM CHES-NaOH, 1.5 mM Diphenylamine
PH: 9
Temperature: 37
- [alanine racemase(Enzyme) wildtype]

L_Alanine -> D_Alanine		 (( (Vmax * A) ) / (Km + A)) buffer: 35 mM CHES-NaOH, 1.5 mM Diphenylamine
PH: 9
Temperature: 65
uniprot:P33162 [Pyridoxal phosphate]

D_Alanine -> L_Alanine		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Sodium pyrophosphate
PH: 8.3
Temperature: 30
uniprot:P33162 [Pyridoxal phosphate]

L_Alanine -> D_Alanine		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Sodium pyrophosphate
PH: 8.3
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) wildtype]

D_Alanine -> L_Alanine		 (( (( (Vf * B) ) / Km1) ) - (( (( (Vr * P) ) / Km2) ) / (( (1 + (B / Km1)) ) + (P / Km2)))) buffer: 100 mM Tricine/NaOH
PH: 9.1
Temperature: 37
- [alanine racemase(Enzyme) wildtype]

D_Alanine -> L_Alanine		 (( (( (Vf * B) ) / Km1) ) - (( (( (Vr * P) ) / Km2) ) / (( (1 + (B / Km1)) ) + (P / Km2)))) buffer: 100 mM Tricine/NaOH
PH: 9.1
Temperature: 37
- [alanine racemase(Enzyme) wildtype]

D_Serine -> L_Serine		 (( (( (Vf * B) ) / Km1) ) - (( (( (Vr * P) ) / Km2) ) / (( (1 + (B / Km1)) ) + (P / Km2)))) buffer: 100 mM Tricine/NaOH
PH: 9.1
Temperature: 37
uniprot:P10724 [alanine racemase(Enzyme) wildtype]

D_Alanine -> L_Alanine		 (( (( (Vf * S) ) / Ks) ) - (( (Vr * P) ) / Kp)) buffer: 50 mM potassium phosphate, 100 mM KCl, 15 µM Pyridoxal 5'-phosphate
PH: 7.9
Temperature: 65
uniprot:P10724 [alanine racemase(Enzyme) wildtype]

D_Alanine -> L_Alanine		 (( (( (Vf * S) ) / Ks) ) - (( (Vr * P) ) / Kp)) buffer: 50 mM potassium borate, 100 mM KCl
PH: 8.6
Temperature: 25
uniprot:P10724 [alanine racemase(Enzyme) wildtype]

D_Alanine -> L_Alanine		 (( (( (Vf * S) ) / Ks) ) - (( (Vr * P) ) / Kp)) buffer: 50 mM potassium borate, 100 mM KCl
PH: 9.4
Temperature: 25
uniprot:P10724 [alanine racemase(Enzyme) wildtype]

L_Serine -> D_Serine		 (( (( (Vf * S) ) / Ks) ) - (( (Vr * P) ) / Kp)) buffer: 50 mM potassium borate, 100 mM KCl
PH: 8.2
Temperature: 25
uniprot:P0A6B4 [alanine racemase(Enzyme) wildtype]

D_Alanine -> L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 10 mM NAD+, 0.5 U/ml L-Alanine dehydrogenase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) mutant P219A]

D_Alanine -> L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 10 mM NAD+, 0.5 U/ml L-Alanine dehydrogenase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) mutant D164K]

D_Alanine -> L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 10 mM NAD+, 0.5 U/ml L-Alanine dehydrogenase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) mutant E221K]

D_Alanine -> L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 10 mM NAD+, 0.5 U/ml L-Alanine dehydrogenase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) mutant E165K]

D_Alanine -> L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 10 mM NAD+, 0.5 U/ml L-Alanine dehydrogenase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) mutant E221A]

D_Alanine -> L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 10 mM NAD+, 0.5 U/ml L-Alanine dehydrogenase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) mutant E165A]

D_Alanine -> L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 10 mM NAD+, 0.5 U/ml L-Alanine dehydrogenase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) mutant D164A]

D_Alanine -> L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 10 mM NAD+, 0.5 U/ml L-Alanine dehydrogenase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) mutant P219A]

L_Alanine -> D_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 0.5 mM NADH, 100 U/ml L-Lactic dehydrogenase, 1 U/ml D-Amino acid oxidase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) wildtype]

L_Alanine -> D_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 0.5 mM NADH, 100 U/ml L-Lactic dehydrogenase, 1 U/ml D-Amino acid oxidase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) mutant E165A]

L_Alanine -> D_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 0.5 mM NADH, 100 U/ml L-Lactic dehydrogenase, 1 U/ml D-Amino acid oxidase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) mutant E221A]

L_Alanine -> D_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 0.5 mM NADH, 100 U/ml L-Lactic dehydrogenase, 1 U/ml D-Amino acid oxidase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) mutant E221P]

D_Alanine -> L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 10 mM NAD+, 0.5 U/ml L-Alanine dehydrogenase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) mutant E221K]

L_Alanine -> D_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 0.5 mM NADH, 100 U/ml L-Lactic dehydrogenase, 1 U/ml D-Amino acid oxidase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) mutant D164A]

L_Alanine -> D_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 0.5 mM NADH, 100 U/ml L-Lactic dehydrogenase, 1 U/ml D-Amino acid oxidase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) mutant E165K]

L_Alanine -> D_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 0.5 mM NADH, 100 U/ml L-Lactic dehydrogenase, 1 U/ml D-Amino acid oxidase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) mutant D164K]

L_Alanine -> D_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 0.5 mM NADH, 100 U/ml L-Lactic dehydrogenase, 1 U/ml D-Amino acid oxidase
PH: 8
Temperature: 30
uniprot:P0A6B4 [alanine racemase(Enzyme) mutant E221P]

L_Alanine -> D_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 0.5 mM NADH, 100 U/ml L-Lactic dehydrogenase, 1 U/ml D-Amino acid oxidase
PH: 8
Temperature: 30
uniprot:Q56257 [alanine racemase(Enzyme) wildtype]

L_Alanine -> D_Alanine		 (( (( (kcat * A) ) * E) ) / (Km + A)) buffer: 20 mM Potassium phosphate
PH: 7.4
Temperature: 25
uniprot:Q56257 [alanine racemase(Enzyme) wildtype]

D_Alanine -> L_Alanine		 (( (( (kcat * A) ) * E) ) / (Km + A)) buffer: 20 mM Potassium phosphate
PH: 7.4
Temperature: 25
uniprot:Q56257 [alanine racemase(Enzyme) wildtype]

L_Alanine_methyl_ester -> D_Alanine_methyl_ester		 (( (( (kcat * A) ) * E) ) / (Km + A)) buffer: 20 mM Potassium phosphate
PH: 7.4
Temperature: 25
uniprot:Q4FME3 [Pyridoxal phosphate]

L_Alanine -> D_Alanine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.2 mM NADH, 1 U/ml D-amino acid oxidase from porcine kidney, 120 U/ml Lactate dehydrogenase from bovine heart
PH: 8.8
Temperature: 37
uniprot:Q73GL9 [Pyridoxal phosphate]

L_Alanine -> D_Alanine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.2 mM NADH, 1 U/ml D-amino acid oxidase from porcine kidney, 120 U/ml Lactate dehydrogenase from bovine heart
PH: 8.8
Temperature: 37
uniprot:Q9X1T3 [Pyridoxal phosphate]

L_Alanine -> D_Alanine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM CHES-NaOH, 1 mM DTT
PH: 9
Temperature: 90
uniprot:Q9X0Z7 [Pyridoxal phosphate]

L_Alanine -> D_Alanine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Bicine
PH: 8
Temperature: 37
uniprot:Q9X0Z7 [Pyridoxal phosphate]

L_Alanine -> D_Alanine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Bicine
PH: 8
Temperature: 70
uniprot:Q9X0Z7 [Pyridoxal phosphate]

L_Alanine -> D_Alanine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8.8
Temperature: 37
uniprot:A0A6C7HVY8 [Pyridoxal phosphate]

L_Alanine -> D_Alanine		 (( (Vmax * S) ) / (Km + S)) buffer: Tris-HCl, 0.2 mM NADH, 0.5 U D-Amino acid aminotransferase, 2 U L-Lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:A0A6C7HRU5 [Pyridoxal phosphate]

D_Alanine -> L_Alanine		 (( (Vmax * S) ) / (Km + S)) buffer: Tris-HCl, 0.2 U L-Alanine dehydrogenase, 2.5 mM NAD+
PH: 8
Temperature: 37
uniprot:A0A6C7HV45 [Pyridoxal phosphate]

L_Alanine -> D_Alanine		 (( (Vmax * S) ) / (Km + S)) buffer: Tris-HCl, 0.2 mM NADH, 0.5 U D-Amino acid aminotransferase, 2 U L-Lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:A0A6C7HV45 [Pyridoxal phosphate]

D_Alanine -> L_Alanine		 (( (Vmax * S) ) / (Km + S)) buffer: Tris-HCl, 0.2 U L-Alanine dehydrogenase, 2.5 mM NAD+
PH: 8
Temperature: 37
uniprot:A0A6C7HVY8 [Pyridoxal phosphate]

D_Alanine -> L_Alanine		 (( (Vmax * S) ) / (Km + S)) buffer: Tris-HCl, 0.2 U L-Alanine dehydrogenase, 2.5 mM NAD+
PH: 8
Temperature: 37
uniprot:A0A6C7HRU5 [Pyridoxal phosphate]

L_Alanine -> D_Alanine		 (( (Vmax * S) ) / (Km + S)) buffer: Tris-HCl, 0.2 mM NADH, 0.5 U D-Amino acid aminotransferase, 2 U L-Lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:Q9X1T3 [Pyridoxal phosphate]

D_Alanine -> L_Alanine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM CHES-NaOH, 1 mM DTT
PH: 9
Temperature: 90

Pathways

pathway id name
BioCyc:META_PWY-6455 vancomycin resistance II
BioCyc:ECOL199310_ALANINE-VALINESYN-PWY alanine biosynthesis I
BioCyc:CAULO_ALANINE-VALINESYN-PWY alanine biosynthesis I
BioCyc:AGRO_ALANINE-VALINESYN-PWY L-alanine biosynthesis I
BioCyc:CORYNE_ALANINE-VALINESYN-PWY L-alanine biosynthesis I
BioCyc:GCF_000013425_PWY-2941 L-lysine biosynthesis II
BioCyc:ECO_ALANINE-VALINESYN-PWY L-alanine biosynthesis I
BioCyc:MTBH37RV_ALANINE-VALINESYN-PWY L-alanine biosynthesis I
BioCyc:HPY_ALADEG-PWY alanine degradation I
BioCyc:ECOO157_ALADEG-PWY alanine degradation I
BioCyc:AGRO_ALADEG-PWY L-alanine degradation I
BioCyc:BSUB_ALANINE-VALINESYN-PWY L-alanine biosynthesis I
BioCyc:ECOL316407_ALANINE-VALINESYN-PWY alanine biosynthesis I
BioCyc:ECOL316407_PWY0-1061 superpathway of alanine biosynthesis
BioCyc:META_PWY0-1061 superpathway of L-alanine biosynthesis
BioCyc:META_ALANINE-VALINESYN-PWY L-alanine biosynthesis I
BioCyc:ECO_PWY0-1061 superpathway of L-alanine biosynthesis
BioCyc:ECOL316407_ALADEG-PWY alanine degradation I
BioCyc:GCF_000013425_ALANINE-VALINESYN-PWY L-alanine biosynthesis I
BioCyc:META_ALADEG-PWY L-alanine degradation I
BioCyc:ECO_ALADEG-PWY L-alanine degradation I
BioCyc:ECOO157_ALANINE-VALINESYN-PWY alanine biosynthesis I
BioCyc:ECOL199310_ALADEG-PWY alanine degradation I
PathBank:SMP0000791 D-Alanine Metabolism
PathBank:SMP0000810 L-Alanine Metabolism