EC: 4.4.1.21

S-ribosylhomocysteine lyase (S-(5-deoxy-D-ribos-5-yl)-L-homocysteine L-homocysteine-lyase [(4S)-4,5-dihydroxypentan-2,3-dione-forming])

enzyme lambda metabolic reaction experiment
uniprot:O34667 [(2S)-2-Amino-4-[(2R,3S)-2,3-dihydroxy-3-N-hydroxycarbamoylpropylmercapto]butyric acid]

S_Ribosyl_L_homocysteine -> 4,5_Dihydroxy_2,3_pentanedione + Homocysteine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:O34667 [(2S)-2-Amino-4-[(2R,3R)-2,3-dihydroxy-3-N-hydroxycarbamoylpropylmercapto]butyric acid]

S_Ribosyl_L_homocysteine -> 4,5_Dihydroxy_2,3_pentanedione + Homocysteine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:O34667 [(2S)-2-Amino-4-[(2R,3R)-2,3-dihydroxy-3-N-hydroxycarbamoylpropylmercapto]butyric acid]

S_Ribosyl_L_homocysteine -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:P45578 [(2S)-2-Amino-4-[(2R,3S)-2,3-dihydroxy-3-N-hydroxycarbamoylpropylmercapto]butyric acid]

S_Ribosyl_L_homocysteine -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:P45578 [(2S)-2-Amino-4-[(2R,3R)-2,3-dihydroxy-3-N-hydroxycarbamoylpropylmercapto]butyric acid]

S_Ribosyl_L_homocysteine -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
- [(2S)-2-Amino-4-[(2R,3S)-2,3-dihydroxy-3-N-hydroxycarbamoylpropylmercapto]butyric acid]

S_Ribosyl_L_homocysteine -> 4,5_Dihydroxy_2,3_pentanedione + Homocysteine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:O34667 [D-Erythronohydroxamic acid]

S_Ribosyl_L_homocysteine -> 4,5_Dihydroxy_2,3_pentanedione + Homocysteine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:O34667 [D-Erythronohydroxamic acid]

S_Ribosyl_L_homocysteine -> 4,5_Dihydroxy_2,3_pentanedione + Homocysteine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:P45578 [D-Erythronohydroxamic acid]

S_Ribosyl_L_homocysteine -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
- [(2S)-2-Amino-4-[(2R,3R)-2,3-dihydroxy-3-N-hydroxycarbamoylpropylmercapto]butyric acid]

S_Ribosyl_L_homocysteine -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:O34667 [S-Ribosylcysteine]

S_Ribosyl_L_homocysteine -> 4,5_Dihydroxy_2,3_pentanedione + Homocysteine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
- [D-Erythronohydroxamic acid]

S_Ribosyl_L_homocysteine -> 4,5_Dihydroxy_2,3_pentanedione + Homocysteine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:O34667 [(2S)-2-Amino-4-[(2R,3S)-2,3-dihydroxy-3-N-hydroxycarbamoylpropylmercapto]butyric acid]

S_Ribosyl_L_homocysteine -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:O34667 [(2S)-2-Amino-4-[(2R,3S)-2,3-dihydroxy-3-N-hydroxycarbamoylpropylmercapto]butyric acid]

S_Ribosyl_L_homocysteine -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:O34667 [(2S)-2-Amino-4-[(2R,3R)-2,3-dihydroxy-3-N-hydroxycarbamoylpropylmercapto]butyric acid]

S_Ribosyl_L_homocysteine -> 4,5_Dihydroxy_2,3_pentanedione + Homocysteine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:O34667 [(2S)-2-Amino-6-(N-formyl-N-hydroxylamino)hexanoic acid]

S_Ribosyl_L_homocysteine -> 4,5_Dihydroxy_2,3_pentanedione + Homocysteine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:O34667 [Methionine]

S_Ribosyl_L_homocysteine -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:O34667 [(2S)-2-Amino-6-(N-mercaptopropionylamido)hexanoic acid]

S_Ribosyl_L_homocysteine -> 4,5_Dihydroxy_2,3_pentanedione + Homocysteine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl
PH: 7
Temperature: 22
uniprot:O34667 [(2S)-2-Amino-6-(N-mercaptobutyrylamido)hexanoic acid]

S_Ribosyl_L_homocysteine -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl
PH: 7
Temperature: 22
uniprot:O34667 [(2S)-2-Amino-6-(N-mercaptoacetamido)hexanoic acid]

S_Ribosyl_L_homocysteine -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl
PH: 7
Temperature: 22
uniprot:O34667 [D-Erythronohydroxamic acid]

S_Ribosyl_L_homocysteine -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM HEPES, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:P45578 [Co2+]

S_Ribosyl_L_homocysteine -> 4,5_Dihydroxy_2,3_pentanedione + Homocysteine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:O34667 [Co2+]

2(S)_Amino_4_[(2S,4S)_2,4,5_trihydroxy_3_oxo_pentylsulfanyl]_butyric_acid -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
uniprot:P45578 [Co2+]

2(S)_Amino_4_[(2S,4S)_2,4,5_trihydroxy_3_oxo_pentylsulfanyl]_butyric_acid -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
- [Co2+]

2(S)_Amino_4_[(2S,4S)_2,4,5_trihydroxy_3_oxo_pentylsulfanyl]_butyric_acid -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
- [Co2+]

2(S)_Amino_4_[(2S,4S)_2,4,5_trihydroxy_3_oxo_pentylsulfanyl]_butyric_acid -> 4,5_Dihydroxy_2,3_pentanedione + Homocysteine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
- [Co2+]

2(S)_Amino_4_[(2S,4S)_2,4,5_trihydroxy_3_oxo_pentylsulfanyl]_butyric_acid -> 4,5_Dihydroxy_2,3_pentanedione + Homocysteine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
- [Co2+]

2(S)_Amino_4_[(2S,4S)_2,4,5_trihydroxy_3_oxo_pentylsulfanyl]_butyric_acid -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
- [Co2+]

2(S)_Amino_4_[(2S,4S)_2,4,5_trihydroxy_3_oxo_pentylsulfanyl]_butyric_acid -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
- [Co2+]

2(S)_Amino_4_[(2S,4S)_2,4,5_trihydroxy_3_oxo_pentylsulfanyl]_butyric_acid -> 4,5_Dihydroxy_2,3_pentanedione + Homocysteine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22
- [Co2+]

2(S)_Amino_4_[(2S,4S)_2,4,5_trihydroxy_3_oxo_pentylsulfanyl]_butyric_acid -> Homocysteine + 4,5_Dihydroxy_2,3_pentanedione		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM NaCl, 150 µM DTNB
PH: 7
Temperature: 22

Pathways

pathway id name
BioCyc:META_PWY-6151 S-adenosyl-L-methionine cycle I
BioCyc:ECO_PWY-6153 autoinducer AI-2 biosynthesis I
BioCyc:HPY_PWY-6153 autoinducer AI-2 biosynthesis I
BioCyc:ECOL199310_PWY-6151 S-adenosyl-L-methionine cycle I
BioCyc:ECOL199310_PWY-6153 autoinducer AI-2 biosynthesis I
BioCyc:BSUB_PWY-6153 autoinducer AI-2 biosynthesis I
BioCyc:ECOL316407_PWY-6151 S-adenosyl-L-methionine cycle I
BioCyc:GCF_000013425_PWY-6151 S-adenosyl-L-methionine cycle I
BioCyc:META_PWY-6153 autoinducer AI-2 biosynthesis I
BioCyc:META_PWY-I9 L-cysteine biosynthesis VI (from L-methionine)
BioCyc:ECO_PWY-6151 S-adenosyl-L-methionine cycle I
BioCyc:HPY_PWY-I9 L-cysteine biosynthesis VI (from L-methionine)
BioCyc:BSUB_PWY-6151 S-adenosyl-L-methionine cycle I
BioCyc:GCF_000013425_PWY-6153 autoinducer AI-2 biosynthesis I
BioCyc:VCHO_PWY-6151 S-adenosyl-L-methionine cycle I
BioCyc:VCHO_PWY-6154 autoinducer AI-2 biosynthesis II (Vibrio)
BioCyc:VCHO_PWY-I9 L-cysteine biosynthesis VI (from L-methionine)
BioCyc:ANTHRA_PWY-6151 S-adenosyl-L-methionine cycle I
BioCyc:ANTHRA_PWY-6153 autoinducer AI-2 biosynthesis I
BioCyc:PDIF272563_PWY-6151 S-adenosyl-L-methionine cycle I
BioCyc:PDIF272563_PWY-6153 autoinducer AI-2 biosynthesis I
PathBank:SMP0000856 Quorum Sensing
PathBank:SMP0002092 S-Adenosyl-L-Methionine Cycle