EC: 4.4.1.1

cystathionine gamma-lyase (L-cystathionine cysteine-lyase (deaminating; 2-oxobutanoate-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q8L0M8 [Pyridoxal phosphate]

L_Cysteine + H2O -> Pyruvate + Hydrogen_sulfide + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37
uniprot:Q8L0M8 [Pyridoxal phosphate]

H2O + L_Cystine -> Pyruvate + NH3 + Thiocysteine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37
uniprot:Q8L0M8 [Pyridoxal phosphate]

H2O + Cystathionine -> L_Homocysteine + Pyruvate + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37
uniprot:Q8L0M8 [Pyridoxal phosphate]

S_(2_Aminoethyl)_L_cysteine + H2O -> NH3 + Cysteamine + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37
uniprot:Q8L0M8 [Pyridoxal phosphate]

3_Chloro_DL_alanine + H2O -> NH3 + Unknown + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37
uniprot:Q8L0M8 [Pyridoxal phosphate]

H2O + S_Methyl_L_cysteine -> NH3 + Pyruvate + Methanethiol		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37
uniprot:P32929 [cystathionine gamma-lyase(Enzyme) wildtype]

L_Cystathionine + H2O -> NH3 + L_Cysteine + 2_Oxobutyrate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Hepes, 1 mM Dithiobisnitrobenzene
PH: 7.4
Temperature: 37
uniprot:P32929 [cystathionine gamma-lyase(Enzyme) wildtype]

H2O + L_Homocysteine -> 2_Oxobutyrate + NH3 + Hydrogen_sulfide		 (( (( (kcat * E) ) * S) ) / (Km + (S * (1 + (S / Ki))))) buffer: 100 mM Hepes, 0.4 mM lead acetate
PH: 7.4
Temperature: 37
uniprot:P32929 [cystathionine gamma-lyase(Enzyme) wildtype]

H2O + L_Cysteine -> NH3 + Hydrogen_sulfide + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + (S * (1 + (S / Ki))))) buffer: 100 mM Hepes, 0.4 mM lead acetate
PH: 7.4
Temperature: 37
uniprot:P32929 [cystathionine gamma-lyase(Enzyme) wildtype]

H2O + L_Cysteine -> Pyruvate + NH3 + Hydrogen_sulfide		 (( (( (kcat * E) ) * S) ) / (Km + (S * (1 + (S / Ki))))) buffer: 100 mM Hepes, 0.4 mM lead acetate
PH: 7.4
Temperature: 37
uniprot:O05394 [Pyridoxal phosphate]

H2O + L_Cystathionine -> NH3 + L_Cysteine + 2_Oxobutyrate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.2 mM DTNB
PH: 9
Temperature: 30
uniprot:F4K5T2 [Pyridoxal phosphate]

H2O + L_Cysteine -> NH3 + Hydrogen_sulfide + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 1 mM dithiothreitol
PH: 8
Temperature: 37
uniprot:P32929 [cystathionine gamma-lyase(Enzyme) wildtype]

L_Cysteine + H2O -> Pyruvate + NH3 + Hydrogen_sulfide		 (( (Vmax * (S ^ n)) ) / (K_half + (S ^ n))) buffer: 2 mM pyridoxal-5'-phosphate
PH: 7
Temperature: 37
uniprot:P32929 [cystathionine gamma-lyase(Enzyme) wildtype]

H2O + L_Cystathionine -> L_Cysteine + 2_Oxobutyrate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Borate, 1 mM DTNB
PH: 8.2
Temperature: 30
uniprot:P32929 [cystathionine gamma-lyase(Enzyme) wildtype]

L_Cystathionine + H2O -> L_Cysteine + NH3 + 2_Oxobutyrate		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Borate, 1 mM DTNB
PH: 8.2
Temperature: 30
uniprot:Q8VCN5 [cystathionine gamma-lyase(Enzyme) wildtype]

L_Cystathionine + H2O -> L_Cysteine + 2_Oxobutyrate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 mM Tris-HCl, 20 µM Pyridoxal-5-phosphate, 1 mM Dithioerythritol
PH: 8.1
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-801 homocysteine and cysteine interconversion
BioCyc:META_PWY-5328 superpathway of L-methionine salvage and degradation
BioCyc:ARA_CYSTSYN-PWY L-cysteine biosynthesis I
BioCyc:ARA_PWY-801 L-homocysteine and L-cysteine interconversion
BioCyc:META_PWY66-426 hydrogen sulfide biosynthesis II (mammalian)
BioCyc:META_PWY-6293 superpathway of L-cysteine biosynthesis (fungi)
BioCyc:META_PWY-821 superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)
BioCyc:ARA_HOMOCYSDEGR-PWY L-cysteine biosynthesis III (from L-homocysteine)
BioCyc:MOUSE_PWY-5328 superpathway of methionine degradation
BioCyc:MOUSE_PWY-6292 cysteine biosynthesis III (mammalia)
BioCyc:HUMAN_PWY66-426 hydrogen sulfide biosynthesis II (mammalian)
BioCyc:META_PWY-6292 superpathway of L-cysteine biosynthesis (mammalian)
BioCyc:MOUSE_HOMOCYSDEGR-PWY cysteine biosynthesis/homocysteine degradation
BioCyc:META_HOMOCYSDEGR-PWY L-cysteine biosynthesis III (from L-homocysteine)
BioCyc:MOUSE_CYSTEINE-SYN2-PWY cysteine biosynthesis II
Reactome:R-CEL-1614635 Sulfur amino acid metabolism
Reactome:R-HSA-1614558 Degradation of cysteine and homocysteine
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-1614558 Degradation of cysteine and homocysteine
Reactome:R-RNO-1614558 Degradation of cysteine and homocysteine
Reactome:R-SCE-71291 Amino acid and derivative metabolism
Reactome:R-SCE-1614603 Cysteine formation from homocysteine
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-DDI-1614558 Degradation of cysteine and homocysteine
Reactome:R-HSA-1614603 Cysteine formation from homocysteine
Reactome:R-HSA-2408508 Metabolism of ingested SeMet, Sec, MeSec into H2Se
Reactome:R-MMU-1614603 Cysteine formation from homocysteine
Reactome:R-RNO-1614603 Cysteine formation from homocysteine
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-1614635 Sulfur amino acid metabolism
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-CEL-1614603 Cysteine formation from homocysteine
Reactome:R-DDI-1614635 Sulfur amino acid metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-SCE-1614558 Degradation of cysteine and homocysteine
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-1614558 Degradation of cysteine and homocysteine
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-1614603 Cysteine formation from homocysteine
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-1614635 Sulfur amino acid metabolism
Reactome:R-HSA-2408522 Selenoamino acid metabolism
Reactome:R-MMU-1614635 Sulfur amino acid metabolism
Reactome:R-RNO-1614635 Sulfur amino acid metabolism
WikiPathways:WP5355 Metabolic Epileptic Disorders
WikiPathways:WP4504 Cysteine and methionine catabolism
PathBank:SMP0120654 Cystinosis, Ocular Nonnephropathic
PathBank:SMP0120694 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0120718 Hypermethioninemia
PathBank:SMP0120754 Non-Ketotic Hyperglycinemia
PathBank:SMP0120770 Hyperglycinemia, Non-Ketotic
PathBank:SMP0120798 gamma-Cystathionase Deficiency (CTH)
PathBank:SMP0000177 Cystathionine beta-Synthase Deficiency
PathBank:SMP0000340 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0000242 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0000223 Non-Ketotic Hyperglycinemia
PathBank:SMP0000484 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0000722 Cystinosis, Ocular Nonnephropathic
PathBank:SMP0002382 Sulfur Metabolism
PathBank:SMP0087161 Selenoamino Acid Metabolism
PathBank:SMP0087189 Cysteine Metabolism
PathBank:SMP0087201 Homocysteine Degradation
PathBank:SMP0087290 Cysteine Metabolism
PathBank:SMP0087298 Homocysteine Degradation
PathBank:SMP0087338 Glycine and Serine Metabolism
PathBank:SMP0087342 Methionine Metabolism
PathBank:SMP0087466 Glycine and Serine Metabolism
PathBank:SMP0120653 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0120741 Sarcosinemia
PathBank:SMP0120853 Homocystinuria-Megaloblastic Anemia Due to Defect in Cobalamin Metabolism, cblG Complementation Type
PathBank:SMP0120873 Cystinosis, Ocular Nonnephropathic
PathBank:SMP0120471 Cystathionine beta-Synthase Deficiency
PathBank:SMP0120475 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0120487 Glycine N-Methyltransferase Deficiency
PathBank:SMP0120519 Methionine Adenosyltransferase Deficiency
PathBank:SMP0120527 S-Adenosylhomocysteine (SAH) Hydrolase Deficiency
PathBank:SMP0120564 beta-Mercaptolactate-Cysteine Disulfiduria
PathBank:SMP0120580 Homocystinuria, Cystathionine beta-Synthase Deficiency
PathBank:SMP0000004 Glycine and Serine Metabolism
PathBank:SMP0000033 Methionine Metabolism
PathBank:SMP0000455 Homocysteine Degradation
PathBank:SMP0000222 Glycine N-Methyltransferase Deficiency
PathBank:SMP0000499 beta-Mercaptolactate-Cysteine Disulfiduria
PathBank:SMP0000515 Homocystinuria, Cystathionine beta-Synthase Deficiency
PathBank:SMP0000721 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0087245 Glycine and Serine Metabolism
PathBank:SMP0087249 Methionine Metabolism
PathBank:SMP0087273 Selenoamino Acid Metabolism
PathBank:SMP0087437 Homocysteine Degradation
PathBank:SMP0063587 Selenoamino Acid Metabolism
PathBank:SMP0063607 Cysteine Metabolism
PathBank:SMP0063637 Methionine Metabolism
PathBank:SMP0120872 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0120521 Sarcosinemia
PathBank:SMP0120634 Homocystinuria-Megaloblastic Anemia Due to Defect in Cobalamin Metabolism, cblG Complementation Type
PathBank:SMP0000013 Cysteine Metabolism
PathBank:SMP0000341 Hypermethioninemia
PathBank:SMP0000221 Methionine Adenosyltransferase Deficiency
PathBank:SMP0000179 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0000485 Hyperglycinemia, Non-Ketotic
PathBank:SMP0002309 Cysteine Metabolism
PathBank:SMP0063628 Homocysteine Degradation
PathBank:SMP0120691 Cystathionine beta-Synthase Deficiency
PathBank:SMP0120695 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0120707 Glycine N-Methyltransferase Deficiency
PathBank:SMP0120739 Methionine Adenosyltransferase Deficiency
PathBank:SMP0120747 S-Adenosylhomocysteine (SAH) Hydrolase Deficiency
PathBank:SMP0120783 beta-Mercaptolactate-Cysteine Disulfiduria
PathBank:SMP0120799 Homocystinuria, Cystathionine beta-Synthase Deficiency
PathBank:SMP0120474 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0120498 Hypermethioninemia
PathBank:SMP0120534 Non-Ketotic Hyperglycinemia
PathBank:SMP0120550 Hyperglycinemia, Non-Ketotic
PathBank:SMP0120579 gamma-Cystathionase Deficiency (CTH)
PathBank:SMP0000029 Selenoamino Acid Metabolism
PathBank:SMP0000214 S-Adenosylhomocysteine (SAH) Hydrolase Deficiency
PathBank:SMP0000244 Sarcosinemia
PathBank:SMP0000514 gamma-Cystathionase Deficiency (CTH)
PathBank:SMP0000570 Homocystinuria-Megaloblastic Anemia Due to Defect in Cobalamin Metabolism, cblG Complementation Type
PathBank:SMP0002371 Selenocompound Metabolism
PathBank:SMP0087423 Selenoamino Acid Metabolism
PathBank:SMP0087483 Methionine Metabolism
PathBank:SMP0063626 Glycine and Serine Metabolism