EC: 4.3.1.19

threonine ammonia-lyase (L-threonine ammonia-lyase (2-oxobutanoate-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q9ZSS6 [Isoleucine]

L_Threonine -> NH3 + 2_Oxobutyrate		 (( (Vi * (S ^ n)) ) / (K_half_inhib + (S ^ n))) buffer: 100 mM KH2/K2HPO4
PH: 8
Temperature: 25
uniprot:Q9ZSS6 [Isoleucine]

L_Threonine -> NH3 + 2_Oxobutyrate		 (( (Vi * (I ^ n)) ) / (K_half_inhib + (I ^ n))) buffer: 100 mM KH2/K2HPO4
PH: 8
Temperature: 25
uniprot:Q9ZSS6 [Isoleucine]

L_Threonine -> NH3 + 2_Oxobutyrate		 (( (Vi * (S ^ n)) ) / (K_half_inhib + (S ^ n))) buffer: 100 mM KH2/K2HPO4
PH: 8
Temperature: 25
uniprot:Q9ZSS6 [Pyridoxal phosphate]

L_Threonine -> 2_Oxobutyrate + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM KH2/K2HPO4
PH: 8
Temperature: 25
uniprot:Q9ZSS6 [Pyridoxal phosphate]

L_Threonine -> NH3 + 2_Oxobutyrate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM KH2/K2HPO4
PH: 8
Temperature: 25
uniprot:Q9ZSS6 [Pyridoxal phosphate]

L_Threonine -> NH3 + 2_Oxobutyrate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM KH2/K2HPO4
PH: 8
Temperature: 25
uniprot:P37946 [Isoleucine]

L_Threonine -> 2_Oxobutyrate + NH3		 (( (Vmax * (S ^ 2)) ) / (( (( (Km ^ 2) ) + (( (2 * b) ) * (S - Km))) ) + (S ^ 2))) buffer: 100 mM potassium phosphate, 0.2 mM NADH, 0.4 mg/ml bovine heart lactic acid dehydrogenase
PH: 8.2
Temperature: 37
uniprot:P37946 [Isoleucine]

L_Threonine -> NH3 + 2_Oxobutyrate		 (( (Vmax * (S ^ 2)) ) / (( (( (Km ^ 2) ) + (( (2 * b) ) * (S - Km))) ) + (S ^ 2))) buffer: 100 mM potassium phosphate, 0.2 mM NADH, 0.4 mg/ml bovine heart lactic acid dehydrogenase
PH: 8.2
Temperature: 37
uniprot:P37946 [threonine ammonia-lyase(Enzyme) wildtype]

L_Threonine -> NH3 + 2_Oxobutyrate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 0.2 mM NADH, 0.4 mg/ml bovine heart lactic acid dehydrogenase
PH: 8.2
Temperature: 37
uniprot:Q9ZSS6 [Isoleucine]

L_Threonine -> 2_Oxobutyrate + NH3		 (( (Vmax * (S ^ n)) ) / (K_half + (S ^ n))) buffer: 100 mM KH2/K2HPO4
PH: 8
Temperature: 25
uniprot:Q9ZSS6 [Isoleucine]

L_Threonine -> NH3 + 2_Oxobutyrate		 (( (Vmax * (S ^ n)) ) / (K_half + (S ^ n))) buffer: 100 mM KH2/K2HPO4
PH: 8
Temperature: 25
uniprot:Q9ZSS6 [Isoleucine]

L_Threonine -> NH3 + 2_Oxobutyrate		 (( (Vmax * (S ^ n)) ) / (K_half + (S ^ n))) buffer: 100 mM KH2/K2HPO4
PH: 8
Temperature: 25
uniprot:Q9ZSS6 [Isoleucine]

L_Threonine -> NH3 + 2_Oxobutyrate		 (( (Vmax * A) ) / (Km_a + A)) buffer: 100 mM KH2/K2HPO4
PH: 8
Temperature: 25
uniprot:Q9ZSS6 [Isoleucine]

L_Threonine -> 2_Oxobutyrate + NH3		 (( (Vmax * A) ) / (Km_a + A)) buffer: 100 mM KH2/K2HPO4
PH: 8
Temperature: 25
uniprot:P11954 [AMP]

L_Threonine -> NH3 + 2_Oxobutyrate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 µM potassium phosphate
PH: 8
Temperature: 25
uniprot:P11954 [CMP]

L_Threonine -> NH3 + 2_Oxobutyrate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 µM potassium phosphate
PH: 8
Temperature: 25
uniprot:P11954 [Pyridoxal phosphate]

L_Threonine -> 2_Oxobutyrate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 100 µM potassium phosphate
PH: 8
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_SERDEG-PWY L-serine degradation
BioCyc:MTBH37RV_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:MTBH37RV_PWY-3001 superpathway of L-isoleucine biosynthesis I
BioCyc:ECOL316407_SERDEG-PWY L-serine degradation
BioCyc:CORYNE_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:CORYNE_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched amino acid biosynthesis
BioCyc:GCF_000013425_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:META_PWY66-428 L-threonine degradation V
BioCyc:MTBCDC1551_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched amino acid biosynthesis
BioCyc:MOUSE_SERDEG-PWY L-serine degradation
BioCyc:GCF_000013425_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:HUMAN_PWY66-428 threonine degradation
BioCyc:MTBH37RV_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:MTBCDC1551_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:ECOL316407_ILEUSYN-PWY isoleucine biosynthesis I (from threonine)
BioCyc:ECOL316407_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:ECOL316407_PWY-5437 threonine degradation I
BioCyc:YEAST_SERDEG-PWY L-serine degradation
BioCyc:MOUSE_PWY-3661 glycine betaine degradation
BioCyc:ECOL316407_THREOCAT-PWY superpathway of threonine metabolism
BioCyc:CORYNE_PWY-3001 superpathway of L-isoleucine biosynthesis I
PathBank:SMP0000838 Isoleucine Biosynthesis
PathBank:SMP0000957 Propanoate Metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SCE-71291 Amino acid and derivative metabolism
Reactome:R-HSA-977347 Serine metabolism
Reactome:R-HSA-8849175 Threonine catabolism
Reactome:R-MMU-977347 Serine metabolism
Reactome:R-MMU-8849175 Threonine catabolism
Reactome:R-RNO-977347 Serine metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-RNO-8849175 Threonine catabolism
Reactome:R-SCE-977347 Serine metabolism
Reactome:R-SCE-8849175 Threonine catabolism
PathBank:SMP0000009 Ammonia Recycling
PathBank:SMP0000242 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0000223 Non-Ketotic Hyperglycinemia
PathBank:SMP0000484 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0087177 Ammonia Recycling
PathBank:SMP0087262 Threonine and 2-Oxobutanoate Degradation
PathBank:SMP0120653 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0120475 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0000004 Glycine and Serine Metabolism
PathBank:SMP0000721 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0002323 Serine Metabolism
PathBank:SMP0087245 Glycine and Serine Metabolism
PathBank:SMP0120521 Sarcosinemia
PathBank:SMP0000179 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0000485 Hyperglycinemia, Non-Ketotic
PathBank:SMP0002430 Isoleucine Biosynthesis
PathBank:SMP0120474 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0120534 Non-Ketotic Hyperglycinemia
PathBank:SMP0120550 Hyperglycinemia, Non-Ketotic
PathBank:SMP0000452 Threonine and 2-Oxobutanoate Degradation
PathBank:SMP0000244 Sarcosinemia
PathBank:SMP0002444 Threonine Metabolism
PathBank:SMP0063626 Glycine and Serine Metabolism