EC: 4.2.1.119

enoyl-CoA hydratase 2 ((3R)-3-hydroxyacyl-CoA hydro-lyase)

enzyme lambda metabolic reaction experiment
uniprot:P51659 [enoyl-CoA hydratase 2(Enzyme) wildtype]

H2O + trans_Dec_2_enoyl_CoA -> (3R)_Hydroxydecanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate, 2 mM Mercaptoethanol.
PH: 7.5
Temperature: 30
uniprot:P51659 [enoyl-CoA hydratase 2(Enzyme) wildtype]

trans_Oct_2_enoyl_CoA + H2O -> (R)_3_Hydroxyoctanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate, 2 mM Mercaptoethanol.
PH: 7.5
Temperature: 30
uniprot:P51659 [enoyl-CoA hydratase 2(Enzyme) wildtype]

H2O + 2_trans_Dodecenoyl_CoA -> (R)_3_Hydroxydodecanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate, 2 mM Mercaptoethanol.
PH: 7.5
Temperature: 30
uniprot:P51659 [enoyl-CoA hydratase 2(Enzyme) wildtype]

H2O + trans_Hex_2_enoyl_CoA -> (R)_3_Hydroxyhexanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate, 2 mM Mercaptoethanol.
PH: 7.5
Temperature: 30
uniprot:P51659 [enoyl-CoA hydratase 2(Enzyme) wildtype]

H2O + trans_Hex_2_enoyl_CoA -> (R)_3_Hydroxyhexanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate, 2 mM Mercaptoethanol.
PH: 7.5
Temperature: 30
uniprot:P51659 [enoyl-CoA hydratase 2(Enzyme) wildtype]

H2O + trans_Dec_2_enoyl_CoA -> (3R)_Hydroxydecanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate, 2 mM Mercaptoethanol.
PH: 7.5
Temperature: 30
uniprot:P51659 [enoyl-CoA hydratase 2(Enzyme) wildtype]

H2O + trans_Oct_2_enoyl_CoA -> (R)_3_Hydroxyoctanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate, 2 mM Mercaptoethanol.
PH: 7.5
Temperature: 30
uniprot:P51659 [enoyl-CoA hydratase 2(Enzyme) wildtype]

2_trans_Dodecenoyl_CoA + H2O -> (R)_3_Hydroxydodecanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate, 2 mM Mercaptoethanol.
PH: 7.5
Temperature: 30
uniprot:Q2RQ36 [enoyl-CoA hydratase 2(Enzyme) wildtype]

Crotonoyl_CoA + H2O -> (R)_3_Hydroxybutanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM TAPS
PH: 8.5
Temperature: 36
uniprot:Q2RQ36 [enoyl-CoA hydratase 2(Enzyme) wildtype]

trans_Hex_2_enoyl_CoA + H2O -> (R)_3_Hydroxyhexanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM TAPS
PH: 8.5
Temperature: 36
uniprot:Q2RQ36 [enoyl-CoA hydratase 2(Enzyme) wildtype]

H2O + trans_Pent_2_enoyl_CoA -> (R)_3_Hydroxypentanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM TAPS
PH: 8.5
Temperature: 36
uniprot:O32472 [enoyl-CoA hydratase 2(Enzyme) wildtype]

Crotonoyl_CoA + H2O -> (R)_3_Hydroxybutanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 30
uniprot:O32472 [enoyl-CoA hydratase 2(Enzyme) wildtype]

H2O + trans_Pent_2_enoyl_CoA -> (R)_3_Hydroxypentanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 30
uniprot:O32472 [enoyl-CoA hydratase 2(Enzyme) wildtype]

H2O + trans_Oct_2_enoyl_CoA -> (R)_3_Hydroxyoctanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 30
uniprot:O32472 [enoyl-CoA hydratase 2(Enzyme) wildtype]

trans_Hex_2_enoyl_CoA + H2O -> (R)_3_Hydroxyhexanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-7339 10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast)
BioCyc:META_PWY-7338 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast)
BioCyc:YEAST_PWY-7288 fatty acid β-oxidation (peroxisome)
BioCyc:META_PWY-7340 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast)
BioCyc:META_PWY-7337 10-cis-heptadecenoyl-CoA degradation (yeast)
BioCyc:YEAST_PWY-7338 10-trans-heptadecenoyl-CoA degradation (reductase-dependent)
BioCyc:YEAST_PWY-7339 10-trans-heptadecenoyl-CoA degradation (MFE-dependent)
BioCyc:ARA_PWY-5138 unsaturated, even numbered fatty acid β-oxidation
BioCyc:META_PWY-7288 fatty acid β-oxidation (peroxisome, yeast)
BioCyc:YEAST_PWY-7337 10-cis-heptadecenoyl-CoA degradation
BioCyc:HUMAN_PWY66-391 fatty acid β-oxidation (peroxisome)
BioCyc:META_PWY66-391 fatty acid β-oxidation VI (peroxisome)
BioCyc:YEAST_YEAST-FAO-PWY fatty acid oxidation (non-cyclic)
BioCyc:YEAST_PWY-7340 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent)
BioCyc:MOUSE_FAO-PWY fatty acid β-oxidation I
BioCyc:MOUSE_PWY-5136 fatty acid β-oxidation II (core pathway)
PlantCyc:PLANT_PWY-5138 unsaturated, even numbered fatty acid β-oxidation
PlantCyc:ARA_PWY-5138 unsaturated, even numbered fatty acid β-oxidation
Reactome:R-DDI-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-DDI-390918 Peroxisomal lipid metabolism
Reactome:R-DDI-192105 Synthesis of bile acids and bile salts
Reactome:R-DME-390918 Peroxisomal lipid metabolism
Reactome:R-DME-194068 Bile acid and bile salt metabolism
Reactome:R-HSA-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-HSA-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-HSA-194068 Bile acid and bile salt metabolism
Reactome:R-HSA-9033500 TYSND1 cleaves peroxisomal proteins
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-MMU-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-RNO-8978868 Fatty acid metabolism
Reactome:R-RNO-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-RNO-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-DDI-8978868 Fatty acid metabolism
Reactome:R-DDI-390247 Beta-oxidation of very long chain fatty acids
Reactome:R-DDI-8957322 Metabolism of steroids
Reactome:R-DDI-194068 Bile acid and bile salt metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-DME-390247 Beta-oxidation of very long chain fatty acids
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-HSA-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-HSA-9033241 Peroxisomal protein import
Reactome:R-MMU-8978868 Fatty acid metabolism
Reactome:R-MMU-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-RNO-390918 Peroxisomal lipid metabolism
Reactome:R-RNO-192105 Synthesis of bile acids and bile salts
Reactome:R-DDI-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-DDI-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-DME-8978868 Fatty acid metabolism
Reactome:R-DME-8957322 Metabolism of steroids
Reactome:R-DME-192105 Synthesis of bile acids and bile salts
Reactome:R-HSA-390247 Beta-oxidation of very long chain fatty acids
Reactome:R-HSA-192105 Synthesis of bile acids and bile salts
Reactome:R-HSA-9609507 Protein localization
Reactome:R-MMU-390247 Beta-oxidation of very long chain fatty acids
Reactome:R-MMU-194068 Bile acid and bile salt metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-RNO-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-556833 Metabolism of lipids
Reactome:R-DDI-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-DME-556833 Metabolism of lipids
Reactome:R-DME-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-DME-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-DME-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-390918 Peroxisomal lipid metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-MMU-390918 Peroxisomal lipid metabolism
Reactome:R-MMU-192105 Synthesis of bile acids and bile salts
Reactome:R-RNO-390247 Beta-oxidation of very long chain fatty acids
Reactome:R-RNO-194068 Bile acid and bile salt metabolism
WikiPathways:WP1941 Peroxisomal beta-oxidation of tetracosanoyl-CoA
PathBank:SMP0120468 Congenital Bile Acid Synthesis Defect Type III
PathBank:SMP0000318 Congenital Bile Acid Synthesis Defect Type III
PathBank:SMP0002324 Biosynthesis of Unsaturated Fatty Acids
PathBank:SMP0002346 Biosynthesis of Unsaturated Fatty Acids (Icosanoyl-CoA)
PathBank:SMP0120463 Cerebrotendinous Xanthomatosis (CTX)
PathBank:SMP0120467 Congenital Bile Acid Synthesis Defect Type II
PathBank:SMP0120495 Zellweger Syndrome
PathBank:SMP0000035 Bile Acid Biosynthesis
PathBank:SMP0000314 Congenital Bile Acid Synthesis Defect Type II
PathBank:SMP0000315 Cerebrotendinous Xanthomatosis (CTX)
PathBank:SMP0002329 Biosynthesis of Unsaturated Fatty Acids (Docosanoyl-CoA)
PathBank:SMP0002368 Fatty Acid Elongation In Mitochondria
PathBank:SMP0120652 27-Hydroxylase Deficiency
PathBank:SMP0120477 Familial Hypercholanemia (FHCA)
PathBank:SMP0000317 Familial Hypercholanemia (FHCA)
PathBank:SMP0002325 Biosynthesis of Unsaturated Fatty Acids (Tetracosanoyl-CoA)
PathBank:SMP0002347 Biosynthesis of Unsaturated Fatty Acids (Stearoyl-CoA)
PathBank:SMP0002434 Lysine Metabolism
PathBank:SMP0000316 Zellweger Syndrome
PathBank:SMP0000720 27-Hydroxylase Deficiency
PathBank:SMP0063601 Bile Acid Biosynthesis