EC: 4.1.1.18

lysine decarboxylase (L-lysine carboxy-lyase (cadaverine-forming))

enzyme lambda metabolic reaction experiment
uniprot:O50657 [Pyridoxal phosphate]

L_Lysine -> CO2 + Cadaverine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.5 M Sodium acetate
PH: 6
Temperature: 40
- [lysine decarboxylase(Enzyme) wildtype]

L_Lysine -> CO2 + Cadaverine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM phosphate, 0.5 mM EDTA, 5 mM dithiothreitol
PH: 7.5
Temperature: 37
- [lysine decarboxylase(Enzyme) wildtype]

L_Lysine -> Cadaverine + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Pyrophosphate, 0.1 mM Pyridoxal-5'-phosphate, 1 mM Dithioerythritol, 10 mM Diethyldithiocarbamate
PH: 8
Temperature: 37
uniprot:Q82VZ0 [Pyridoxal phosphate]

L_Lysine -> CO2 + Cadaverine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris, 0.08 mg/ml BSA, 10 mM MgCl2, 0.256 mM NADH, 1.76 mM phosphoenol pyruvate, 4 mM DTT, 1.5% glycerol, 0.77 U/ml malate dehydrogenase, 0.14 U/ml phosphoenol pyruvate carboxykinase
PH: 8
Temperature: 37
uniprot:A0A3Q0KZG4 [Pyridoxal phosphate]

L_Lysine -> CO2 + Cadaverine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris, 0.08 mg/ml BSA, 10 mM MgCl2, 0.256 mM NADH, 1.76 mM phosphoenol pyruvate, 4 mM DTT, 1.5% glycerol, 0.77 U/ml malate dehydrogenase, 0.14 U/ml phosphoenol pyruvate carboxykinase
PH: 8
Temperature: 37
uniprot:Q9X2I6 [Pyridoxal phosphate]

L_Lysine -> Cadaverine + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM HEPES, 50 mM NaCl, 5 mM DTT
PH: 8.2
Temperature: 63
uniprot:O66940 [Pyridoxal phosphate]

L_Lysine -> Cadaverine + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM HEPES, 50 mM NaCl, 5 mM DTT
PH: 7
Temperature: 63
uniprot:Q9HVQ3 [Pyridoxal phosphate]

L_Lysine -> Cadaverine + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris, 0.08 mg/ml BSA, 10 mM MgCl2, 0.256 mM NADH, 1.76 mM phosphoenol pyruvate, 4 mM DTT, 1.5% glycerol, 0.77 U/ml malate dehydrogenase, 0.14 U/ml phosphoenol pyruvate carboxykinase
PH: 8
Temperature: 37
uniprot:A0A0R4J8C9 [Pyridoxal phosphate]

L_Lysine -> CO2 + Cadaverine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris, 0.08 mg/ml BSA, 10 mM MgCl2, 0.256 mM NADH, 1.76 mM phosphoenol pyruvate, 4 mM DTT, 1.5% glycerol, 0.77 U/ml malate dehydrogenase, 0.14 U/ml phosphoenol pyruvate carboxykinase
PH: 8
Temperature: 37
uniprot:Q8PS97 [Pyridoxal phosphate]

L_Lysine -> CO2 + Cadaverine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris, 0.08 mg/ml BSA, 10 mM MgCl2, 0.256 mM NADH, 1.76 mM phosphoenol pyruvate, 4 mM DTT, 1.5% glycerol, 0.77 U/ml malate dehydrogenase, 0.14 U/ml phosphoenol pyruvate carboxykinase
PH: 8
Temperature: 37
uniprot:A8BFD3 [Pyridoxal phosphate]

L_Lysine -> CO2 + Cadaverine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 20 mM Tris, 0.08 mg/ml BSA, 10 mM MgCl2, 0.256 mM NADH, 1.76 mM phosphoenol pyruvate, 4 mM DTT, 1.5% glycerol, 0.77 U/ml malate dehydrogenase, 0.14 U/ml phosphoenol pyruvate carboxykinase
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY0-461 L-lysine degradation I
BioCyc:ECO_PWY0-461 L-lysine degradation I
BioCyc:ECO_POLYAMSYN-PWY superpathway of polyamine biosynthesis I
BioCyc:ECOL316407_ARG+POLYAMINE-SYN superpathway of arginine and polyamine biosynthesis
BioCyc:META_PWY0-1303 aminopropylcadaverine biosynthesis
BioCyc:META_POLYAMSYN-PWY superpathway of polyamine biosynthesis I
BioCyc:ECO_ARG+POLYAMINE-SYN superpathway of arginine and polyamine biosynthesis
BioCyc:META_PWY-5327 superpathway of L-lysine degradation
BioCyc:ECOO157_PWY0-461 lysine degradation I
BioCyc:ECOL316407_PWY0-461 lysine degradation I
BioCyc:META_ARG+POLYAMINE-SYN superpathway of arginine and polyamine biosynthesis
BioCyc:ECO_PWY0-1303 aminopropylcadaverine biosynthesis
BioCyc:ECOL316407_PWY0-1303 aminopropylcadaverine biosynthesis
BioCyc:ECOL316407_POLYAMSYN-PWY superpathway of polyamine biosynthesis I
PathBank:SMP0000795 Lysine Degradation I
PathBank:SMP0002053 Aminopropylcadaverine Biosynthesis