EC: 4.1.1.15

glutamate decarboxylase (L-glutamate 1-carboxy-lyase (4-aminobutanoate-forming))

enzyme lambda metabolic reaction experiment
- [Pyridoxal phosphate]

L_Glutamate -> CO2 + 4_Aminobutanoate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, hyamine hydroxide - inner well
PH: 5.8
Temperature: 37
uniprot:Q05329 [Pyridoxal phosphate]

L_Glutamate -> 4_Aminobutanoate + CO2		 (( (Vmax * M) ) / (Km + M)) buffer: 50 mM HEPES/NaOH, pH 7.0, 1 mM 2-aminoethylisothiouronium bromide, 1 mM Phenylmethylsulfonyl fluoride, 10 mM Benzamidine/HCl, 5 mM Sodium fluoride, 0.1% BSA
PH: 7
Temperature: 37
uniprot:Q05329 [Pyridoxal phosphate]

L_Glutamate -> 4_Aminobutanoate + CO2		 (( (Vmax * M) ) / (Km + M)) buffer: 50 mM HEPES/NaOH, pH 7.0, 1 mM 2-aminoethylisothiouronium bromide, 1 mM Phenylmethylsulfonyl fluoride, 10 mM Benzamidine/HCl, 5 mM Sodium fluoride, 0.1% BSA
PH: 7
Temperature: 37
uniprot:Q05683 [Pyridoxal phosphate]

L_Glutamate -> CO2 + 4_Aminobutanoate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES/NaOH, pH 7.0, 1 mM 2-aminoethylisothiouronium bromide, 1 mM Phenylmethylsulfonyl fluoride, 10 mM Benzamidine/HCl, 5 mM Sodium fluoride
PH: 7
Temperature: 37
uniprot:Q05683 [Pyridoxal phosphate]

L_Glutamate -> 4_Aminobutanoate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES/NaOH, pH 7.0, 1 mM 2-aminoethylisothiouronium bromide, 1 mM Phenylmethylsulfonyl fluoride, 10 mM Benzamidine/HCl, 5 mM Sodium fluoride
PH: 7
Temperature: 37
uniprot:Q05683 [Pyridoxal phosphate]

L_Glutamate -> 4_Aminobutanoate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES/NaOH, pH 7.0, 1 mM 2-aminoethylisothiouronium bromide, 1 mM Phenylmethylsulfonyl fluoride, 10 mM Benzamidine/HCl, 5 mM Sodium fluoride
PH: 7
Temperature: 37
uniprot:Q05683 [Pyridoxal phosphate]

L_Glutamate -> 4_Aminobutanoate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES/NaOH, pH 7.0, 1 mM 2-aminoethylisothiouronium bromide, 1 mM Phenylmethylsulfonyl fluoride, 10 mM Benzamidine/HCl, 5 mM Sodium fluoride
PH: 7
Temperature: 37
uniprot:Q05683 [Pyridoxal phosphate]

L_Glutamate -> CO2 + 4_Aminobutanoate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES/NaOH, pH 7.0, 1 mM 2-aminoethylisothiouronium bromide, 1 mM Phenylmethylsulfonyl fluoride, 10 mM Benzamidine/HCl, 5 mM Sodium fluoride
PH: 7
Temperature: 37
uniprot:Q05329 [Pyridoxal phosphate]

L_Glutamate -> 4_Aminobutanoate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES/NaOH, pH 7.0, 1 mM 2-aminoethylisothiouronium bromide, 1 mM Phenylmethylsulfonyl fluoride, 10 mM Benzamidine/HCl, 5 mM Sodium fluoride
PH: 7
Temperature: 37
uniprot:Q05329 [Pyridoxal phosphate]

L_Glutamate -> CO2 + 4_Aminobutanoate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES/NaOH, pH 7.0, 1 mM 2-aminoethylisothiouronium bromide, 1 mM Phenylmethylsulfonyl fluoride, 10 mM Benzamidine/HCl, 5 mM Sodium fluoride
PH: 7
Temperature: 37
uniprot:Q05683 [Pyridoxal phosphate]

L_Glutamate -> 4_Aminobutanoate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES/NaOH, pH 7.0, 1 mM 2-aminoethylisothiouronium bromide, 1 mM Phenylmethylsulfonyl fluoride, 10 mM Benzamidine/HCl, 5 mM Sodium fluoride
PH: 7
Temperature: 37
- [Pyridoxal phosphate]

L_Glutamate -> CO2 + 4_Aminobutanoate		 (( (Vmax * M) ) / (Km + M)) buffer: 50 mM sodium phosphate, hyamine hydroxide - inner well
PH: 5.8
Temperature: 37

Pathways

pathway id name
BioCyc:YEAST_GLUDEG-I-PWY-1 glutamate degradation to succinate
BioCyc:ECOO157_PWY0-1305 glutamate dependent acid resistance
BioCyc:ECOL316407_PWY0-1305 glutamate dependent acid resistance
BioCyc:HUMAN_GLUDEG-I-PWY GABA shunt
BioCyc:HUMAN_PWY0-1305 glutamate dependent acid resistance
BioCyc:META_GLUDEG-I-PWY GABA shunt
BioCyc:ECO_PWY0-1305 L-glutamate degradation IX (via 4-aminobutanoate)
BioCyc:YEAST_PWY0-1305 L-glutamate degradation to 4-aminobutanoate
BioCyc:ECOO157_PWY3O-210 glutamate degradation IX (via 4-aminobutyrate)
BioCyc:META_PWY-4321 L-glutamate degradation IV
BioCyc:ARA_PWY-4321 L-glutamate degradation IV
BioCyc:MOUSE_GLUDEG-I-PWY glutamate degradation III (via 4-aminobutyrate)
BioCyc:META_PWY0-1305 L-glutamate degradation IX (via 4-aminobutanoate)
BioCyc:MOUSE_PWY-4321 glutamate degradation IV
PathBank:SMP0000811 L-Glutamate Metabolism
PlantCyc:TOMATO_PWY-8346 GABA shunt II
PlantCyc:TOMATO_PWY-4321 L-glutamate degradation IV
PlantCyc:ARA_PWY-4321 L-glutamate degradation IV
PlantCyc:PLANT_PWY-4321 L-glutamate degradation IV
PlantCyc:TOMATO_PWY0-1305 L-glutamate degradation IX (via 4-aminobutanoate)
Reactome:R-BTA-112310 Neurotransmitter release cycle
Reactome:R-CFA-112315 Transmission across Chemical Synapses
Reactome:R-CFA-888590 GABA synthesis, release, reuptake and degradation
Reactome:R-DME-112310 Neurotransmitter release cycle
Reactome:R-HSA-112315 Transmission across Chemical Synapses
Reactome:R-HSA-888590 GABA synthesis, release, reuptake and degradation
Reactome:R-HSA-74160 Gene expression (Transcription)
Reactome:R-HSA-73857 RNA Polymerase II Transcription
Reactome:R-HSA-9022927 MECP2 regulates transcription of genes involved in GABA signaling
Reactome:R-RNO-112316 Neuronal System
Reactome:R-RNO-888568 GABA synthesis
Reactome:R-BTA-112316 Neuronal System
Reactome:R-BTA-888568 GABA synthesis
Reactome:R-DME-112315 Transmission across Chemical Synapses
Reactome:R-DME-888568 GABA synthesis
Reactome:R-HSA-8986944 Transcriptional Regulation by MECP2
Reactome:R-MMU-112310 Neurotransmitter release cycle
Reactome:R-RNO-112315 Transmission across Chemical Synapses
Reactome:R-RNO-888590 GABA synthesis, release, reuptake and degradation
Reactome:R-CFA-112316 Neuronal System
Reactome:R-CFA-888568 GABA synthesis
Reactome:R-HSA-112316 Neuronal System
Reactome:R-HSA-888568 GABA synthesis
Reactome:R-HSA-212436 Generic Transcription Pathway
Reactome:R-MMU-112315 Transmission across Chemical Synapses
Reactome:R-MMU-888590 GABA synthesis, release, reuptake and degradation
Reactome:R-RNO-112310 Neurotransmitter release cycle
Reactome:R-BTA-112315 Transmission across Chemical Synapses
Reactome:R-BTA-888590 GABA synthesis, release, reuptake and degradation
Reactome:R-CFA-112310 Neurotransmitter release cycle
Reactome:R-DME-112316 Neuronal System
Reactome:R-DME-888590 GABA synthesis, release, reuptake and degradation
Reactome:R-HSA-112310 Neurotransmitter release cycle
Reactome:R-MMU-112316 Neuronal System
Reactome:R-MMU-888568 GABA synthesis
WikiPathways:WP5298 Dravet syndrome: Scn1a-A1783V point mutation model
WikiPathways:WP4157 GABA metabolism (aka GHB)
PathBank:SMP0120496 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0000339 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0000492 Ureidopropionase Deficiency
PathBank:SMP0087165 Aspartate Metabolism
PathBank:SMP0087169 Glutamate Metabolism
PathBank:SMP0000385 Homocarnosinosis
PathBank:SMP0000136 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0000567 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0087180 beta-Alanine Metabolism
PathBank:SMP0063599 beta-Alanine Metabolism
PathBank:SMP0120445 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120461 Canavan Disease
PathBank:SMP0120558 Carnosinuria, Carnosinemia
PathBank:SMP0000067 Aspartate Metabolism
PathBank:SMP0000175 Canavan Disease
PathBank:SMP0000351 GABA-Transaminase Deficiency
PathBank:SMP0000493 Carnosinuria, Carnosinemia
PathBank:SMP0002303 Glutamate Metabolism
PathBank:SMP0002379 Taurine and Hypotaurine Biosynthesis
PathBank:SMP0063590 Glutamate Metabolism
PathBank:SMP0120438 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120478 GABA-Transaminase Deficiency
PathBank:SMP0120494 Homocarnosinosis
PathBank:SMP0120502 Hypoacetylaspartia
PathBank:SMP0120631 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000007 beta-Alanine Metabolism
PathBank:SMP0000072 Glutamate Metabolism
PathBank:SMP0000021 Taurine and Hypotaurine Metabolism
PathBank:SMP0000243 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000192 Hypoacetylaspartia
PathBank:SMP0002427 Glutamic Acid Metabolism
PathBank:SMP0087214 Taurine and Hypotaurine Metabolism
PathBank:SMP0012467 Butanoate Metabolism
PathBank:SMP0063597 Aspartate Metabolism
PathBank:SMP0063675 Taurine and Hypotaurine Metabolism