EC: 3.6.1.13

ADP-ribose diphosphatase (ADP-D-ribose ribophosphohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) wildtype]

ADPribose + H2O -> AMP + D_Ribose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant R18Q]

H2O + ADPribose -> D_Ribose_5_phosphate + AMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant Y28Q]

ADPribose + H2O -> AMP + D_Ribose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant E63Q]

ADPribose + H2O -> AMP + D_Ribose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant H33A]

ADPribose + H2O -> D_Ribose_5_phosphate + AMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant I19A]

ADPribose + H2O -> AMP + D_Ribose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant R54Q]

H2O + ADPribose -> AMP + D_Ribose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant L68A]

ADPribose + H2O -> AMP + D_Ribose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant Q52A]

ADPribose + H2O -> D_Ribose_5_phosphate + AMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant R27Q]

H2O + ADPribose -> AMP + D_Ribose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant E108Q]

H2O + ADPribose -> D_Ribose_5_phosphate + AMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant R81Q]

ADPribose + H2O -> AMP + D_Ribose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant S102A]

ADPribose + H2O -> AMP + D_Ribose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant T110A]

ADPribose + H2O -> AMP + D_Ribose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant T155A]

ADPribose + H2O -> AMP + D_Ribose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant Y99F]

ADPribose + H2O -> AMP + D_Ribose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant E70Q]

H2O + ADPribose -> D_Ribose_5_phosphate + AMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant E73Q]

H2O + ADPribose -> D_Ribose_5_phosphate + AMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant S153A]

H2O + ADPribose -> D_Ribose_5_phosphate + AMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [F-]

ADPribose + H2O -> AMP + D_Ribose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki1))) ) + (S * (1 + (I / Ki2))))) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) wildtype]

ADPribose + H2O -> AMP + D_Ribose_5_phosphate		 (( (E * kcat_max) ) / (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pK1)))) ) + (( (10 ^ (0 - pK2)) ) / (10 ^ (0 - pH))))) buffer: 50 mM MES-HCl (pH 4.87 - 6.98) / Tris-HCl (pH 7.03 - 9.10) / glycine-NaOH (pH 9.01 - 10.72), 100 mM KCl, 20 mM MgCl2, 50 U/ml calf intestine alkaline phosphatase
PH: 4.87
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant E85Q]

ADPribose + H2O -> D_Ribose_5_phosphate + AMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant E86Q]

H2O + ADPribose -> D_Ribose_5_phosphate + AMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant E82Q]

H2O + ADPribose -> AMP + D_Ribose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 20 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant R54Q]

ADPribose + H2O -> D_Ribose_5_phosphate + AMP		 (( (E * kcat_max) ) / (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pK1))))) buffer: 50 mM MES-HCl (pH 4.87 - 6.98) / Tris-HCl (pH 7.03 - 9.10) / glycine-NaOH (pH 9.01 - 10.72), 100 mM KCl, 20 mM MgCl2, 50 U/ml calf intestine alkaline phosphatase
PH: 4.87
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) wildtype]

H2O + ADP_alpha_D_glucoside -> D_Glucose_1_phosphate + AMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 20 mM MgCl2, 50 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) wildtype]

GDPribose + H2O -> D_Ribose_5_phosphate + GMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 20 mM MgCl2, 50 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) wildtype]

CDPribose + H2O -> D_Ribose_5_phosphate + CMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl, 20 mM MgCl2, 50 U/ml calf intestine alkaline phosphatase
PH: 7.63
Temperature: 25
uniprot:Q5SKW5 [Mn2+]

ADPribose + H2O -> D_Ribose_5_phosphate + AMP		 (( (E * kcat_max) ) / (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pK1)))) ) + (( (10 ^ (0 - pK2)) ) / (10 ^ (0 - pH))))) buffer: 50 mM MES-HCl (pH 4.62 - 6.49) / Tris-HCl (pH 6.90 - 8.95), 100 mM KCl
PH: 4.62
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) wildtype]

ADPribose + H2O -> D_Ribose_5_phosphate + AMP		 (( (E * kcat_max) ) / (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pK1)))) ) + (( (10 ^ (0 - pK2)) ) / (10 ^ (0 - pH))))) buffer: 50 mM MES-HCl (pH 4.62 - 6.49) / Tris-HCl (pH 6.90 - 8.95), 100 mM KCl
PH: 4.62
Temperature: 25
uniprot:Q5SKW5 [Mg2+]

H2O + ADPribose -> D_Ribose_5_phosphate + AMP		 (( (E * kcat_max) ) / (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pK1))))) buffer: 50 mM MES-HCl (pH 4.62 - 6.49) / Tris-HCl (pH 6.90 - 8.95), 100 mM KCl
PH: 4.62
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant E86Q]

H2O + ADPribose -> AMP + D_Ribose_5_phosphate		 (( (E * kcat_max) ) / (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pK1))))) buffer: 50 mM MES-HCl (pH 4.87 - 6.98) / Tris-HCl (pH 7.03 - 9.10) / glycine-NaOH (pH 9.01 - 10.72), 100 mM KCl, 20 mM MgCl2, 50 U/ml calf intestine alkaline phosphatase
PH: 4.87
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant R81Q]

ADPribose + H2O -> D_Ribose_5_phosphate + AMP		 (( (E * kcat_max) ) / (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pK1))))) buffer: 50 mM MES-HCl (pH 4.87 - 6.98) / Tris-HCl (pH 7.03 - 9.10) / glycine-NaOH (pH 9.01 - 10.72), 100 mM KCl, 20 mM MgCl2, 50 U/ml calf intestine alkaline phosphatase
PH: 4.87
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant E82Q]

H2O + ADPribose -> AMP + D_Ribose_5_phosphate		 (( (E * kcat_max) ) / (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pK1))))) buffer: 50 mM MES-HCl (pH 4.87 - 6.98) / Tris-HCl (pH 7.03 - 9.10) / glycine-NaOH (pH 9.01 - 10.72), 100 mM KCl, 20 mM MgCl2, 50 U/ml calf intestine alkaline phosphatase
PH: 4.87
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant E85Q]

H2O + ADPribose -> AMP + D_Ribose_5_phosphate		 (( (E * kcat_max) ) / (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pK1))))) buffer: 50 mM MES-HCl (pH 4.87 - 6.98) / Tris-HCl (pH 7.03 - 9.10) / glycine-NaOH (pH 9.01 - 10.72), 100 mM KCl, 20 mM MgCl2, 50 U/ml calf intestine alkaline phosphatase
PH: 4.87
Temperature: 25
uniprot:Q5SKW5 [ADP-ribose diphosphatase(Enzyme) mutant E70Q]

ADPribose + H2O -> AMP + D_Ribose_5_phosphate		 (( (E * kcat_max) ) / (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pK1))))) buffer: 50 mM MES-HCl (pH 4.87 - 6.98) / Tris-HCl (pH 7.03 - 9.10) / glycine-NaOH (pH 9.01 - 10.72), 100 mM KCl, 20 mM MgCl2, 50 U/ml calf intestine alkaline phosphatase
PH: 4.87
Temperature: 25
- [Mg2+]

ADPribose + H2O -> D_Ribose_5_phosphate + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Glycine-NaOH
PH: 9.5
Temperature: 37
uniprot:Q5XIG0 [Mg2+]

ADPribose + H2O -> AMP + D_Ribose_5_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris, 2.5 µg/ml Alkaline phosphatase, 1 µg/ml Adenosine deaminase
PH: 7.5
Temperature: 37
uniprot:Q5M886 [Mg2+]

H2O + ADPribose -> D_Ribose_5_phosphate + AMP		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2.5 µg/ml Alkaline phosphatase, 1 µg/ml Adenosine deaminase
PH: 7.5
Temperature: 37
uniprot:Q6AY63 [Mg2+]

ADPribose + H2O -> AMP + D_Ribose_5_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2.5 µg/ml Alkaline phosphatase, 1 µg/ml Adenosine deaminase
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:ARA_PWY-5381 pyridine nucleotide cycling (plants)
BioCyc:ECO_PWY0-1597 prenylated FMNH2 biosynthesis
PlantCyc:ARA_PWY-5381 pyridine nucleotide cycling (plants)
Reactome:R-DRE-2393930 Phosphate bond hydrolysis by NUDT proteins
Reactome:R-HSA-2393930 Phosphate bond hydrolysis by NUDT proteins
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-2393930 Phosphate bond hydrolysis by NUDT proteins
Reactome:R-RNO-2393930 Phosphate bond hydrolysis by NUDT proteins
Reactome:R-XTR-15869 Nucleotide metabolism
Reactome:R-XTR-8956319 Nucleotide catabolism
Reactome:R-DRE-74259 Purine catabolism
Reactome:R-HSA-74259 Purine catabolism
Reactome:R-MMU-74259 Purine catabolism
Reactome:R-RNO-74259 Purine catabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-2393930 Phosphate bond hydrolysis by NUDT proteins
Reactome:R-DRE-1430728 Metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-SCE-15869 Nucleotide metabolism
Reactome:R-SCE-8956319 Nucleotide catabolism
Reactome:R-XTR-1430728 Metabolism
Reactome:R-XTR-74259 Purine catabolism
Reactome:R-DRE-15869 Nucleotide metabolism
Reactome:R-DRE-8956319 Nucleotide catabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-HSA-8956319 Nucleotide catabolism
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-MMU-8956319 Nucleotide catabolism
Reactome:R-RNO-15869 Nucleotide metabolism
Reactome:R-RNO-8956319 Nucleotide catabolism
Reactome:R-SCE-74259 Purine catabolism
Reactome:R-XTR-2393930 Phosphate bond hydrolysis by NUDT proteins
PathBank:SMP0120670 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0120669 Adenosine Deaminase Deficiency
PathBank:SMP0120673 AICA-Ribosiduria
PathBank:SMP0120709 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0120717 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0120797 Xanthinuria Type II
PathBank:SMP0120821 Myoadenylate Deaminase Deficiency
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0120728 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0120752 Molybdenum Cofactor Deficiency
PathBank:SMP0120756 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0120796 Xanthinuria Type I
PathBank:SMP0120820 Mitochondrial DNA Depletion Syndrome
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0087332 Purine Metabolism
PathBank:SMP0063462 Riboflavin Metabolism
PathBank:SMP0120819 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000427 Azathioprine Action Pathway