EC: 3.5.4.6

AMP deaminase (AMP aminohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q54DD0 [AMP deaminase(Enzyme) wildtype]

H2O + AMP -> NH3 + IMP
(( (Vmax * S) ) / (Km + S)) buffer: 0.1 mM imidazole-HCl, 0.1 M KCl
PH: 6.5
Temperature: 22
- [Li2SO4]

H2O + AMP -> IMP + NH3
(( (Vmax * A) ) / (Km + A)) buffer: 96 mM Succinate
PH: 6.2
Temperature: 30
uniprot:O80452 [ATP]

AMP + H2O -> IMP + NH3
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Ammonium sulfate, 0.2 µg/µl Bovine serum albumin
PH: 7
Temperature: 36
uniprot:O80452 [AMP deaminase(Enzyme) mutant deltaI139M]

AMP + H2O -> IMP + NH3
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Ammonium sulfate, 0.2 µg/µl Bovine serum albumin
PH: 7
Temperature: 36
uniprot:O80452 [AMP deaminase(Enzyme) mutant deltaL31M]

AMP + H2O -> NH3 + IMP
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Ammonium sulfate, 0.2 µg/µl Bovine serum albumin
PH: 7
Temperature: 36
uniprot:O80452 [ATP]

H2O + AMP -> NH3 + IMP
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Ammonium sulfate, 0.2 µg/µl Bovine serum albumin
PH: 7
Temperature: 36
uniprot:P23109 [ATP]

AMP + H2O -> NH3 + IMP
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Potassium chloride
PH: 6.5
Temperature: 37
uniprot:Q01432 [ATP]

H2O + AMP -> IMP + NH3
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Potassium chloride
PH: 7
Temperature: 37
uniprot:P23109 [ATP]

AMP + H2O -> IMP + NH3
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Potassium chloride
PH: 6.5
Temperature: 37
uniprot:P23109 [AMP deaminase(Enzyme) wildtype AMPD1 (isoform M)]

H2O + AMP -> NH3 + IMP
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Potassium chloride
PH: 6.5
Temperature: 37
uniprot:Q01432 [AMP deaminase(Enzyme) wildtype AMPD3 (isoform E)]

AMP + H2O -> NH3 + IMP
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Potassium chloride
PH: 7
Temperature: 37
uniprot:P23109 [AMP deaminase(Enzyme) mutant AMPD1 deltaL96]

H2O + AMP -> NH3 + IMP
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Potassium chloride
PH: 6.5
Temperature: 37
uniprot:P23109 [ATP]

H2O + AMP -> NH3 + IMP
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Potassium chloride
PH: 6.7
Temperature: 37
uniprot:Q01432 [ATP]

H2O + AMP -> IMP + NH3
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Potassium chloride
PH: 6.7
Temperature: 37
uniprot:Q01432 [ATP]

H2O + AMP -> NH3 + IMP
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Potassium chloride
PH: 6.7
Temperature: 37
uniprot:Q01432 [ATP]

AMP + H2O -> NH3 + IMP
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Potassium chloride
PH: 6.7
Temperature: 37
uniprot:Q01432 [ATP]

H2O + AMP -> NH3 + IMP
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Potassium chloride
PH: 7
Temperature: 37
uniprot:Q01432 [AMP deaminase(Enzyme) wildtype AMPD1/AMPD3 peak II]

AMP + H2O -> IMP + NH3
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Potassium chloride
PH: 6.7
Temperature: 37
uniprot:Q01432 [AMP deaminase(Enzyme) wildtype AMPD1/AMPD3 peak III]

AMP + H2O -> NH3 + IMP
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Potassium chloride
PH: 6.7
Temperature: 37
uniprot:Q01432 [AMP deaminase(Enzyme) wildtype AMPD1/AMPD3 peak I]

AMP + H2O -> NH3 + IMP
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Potassium chloride
PH: 6.7
Temperature: 37
uniprot:Q01432 [AMP deaminase(Enzyme) mutant AMPD3 deltaM90]

H2O + AMP -> NH3 + IMP
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Potassium chloride
PH: 7
Temperature: 37
uniprot:P23109 [AMP deaminase(Enzyme) wildtype AMPD1/AMPD3 peak IV]

AMP + H2O -> NH3 + IMP
(( (Vmax * A) ) / (Km + A)) buffer: 25 mM Imidazole, 150 mM Potassium chloride
PH: 6.7
Temperature: 37
uniprot:Q01433 [Phosphate]

H2O + AMP -> NH3 + IMP
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M sodium-cacodylate
PH: 7
Temperature: 30
uniprot:Q01433 [Phosphate]

AMP + H2O -> NH3 + IMP
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M sodium-cacodylate
PH: 7
Temperature: 30
uniprot:Q01433 [ADP]

AMP + H2O -> IMP + NH3
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M sodium-cacodylate
PH: 7
Temperature: 30
uniprot:Q01433 [ADP]

AMP + H2O -> IMP + NH3
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M sodium-cacodylate
PH: 7
Temperature: 30
uniprot:Q01433 [GTP]

H2O + AMP -> IMP + NH3
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M sodium-cacodylate
PH: 7
Temperature: 30
uniprot:Q01433 [GTP]

AMP + H2O -> NH3 + IMP
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M sodium-cacodylate
PH: 7
Temperature: 30
uniprot:Q01433 [ATP]

H2O + AMP -> NH3 + IMP
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M sodium-cacodylate
PH: 7
Temperature: 30
uniprot:Q01433 [ATP]

H2O + AMP -> IMP + NH3
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M sodium-cacodylate
PH: 7
Temperature: 30
uniprot:Q01433 [AMP deaminase(Enzyme) wildtype isoenzyme AMPD2]

H2O + AMP -> IMP + NH3
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M sodium-cacodylate
PH: 7
Temperature: 30
uniprot:Q01433 [AMP deaminase(Enzyme) wildtype proteolytic isoenzyme AMPD2]

AMP + H2O -> IMP + NH3
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M sodium-cacodylate
PH: 7
Temperature: 30

Pathways

pathway id name
BioCyc:ARA_PWY-6596 adenosine nucleotides degradation I
BioCyc:YEAST_PWY3O-2220 salvage pathways of adenine, hypoxanthine and their nucleosides
BioCyc:YEAST_PWY3O-1 superpathway of purine nucleosides salvage
PlantCyc:ARA_P165-PWY superpathway of purines degradation in plants
PlantCyc:PLANT_P165-PWY superpathway of purines degradation in plants
PlantCyc:ARA_PWY-6596 adenosine nucleotides degradation I
PlantCyc:PLANT_PWY-6596 adenosine nucleotides degradation I
PlantCyc:PLANT_PWY-5044 purine nucleotides degradation I (plants)
PlantCyc:ARA_PWY-5044 purine nucleotides degradation I (plants)
Reactome:R-DDI-8956321 Nucleotide salvage
Reactome:R-HSA-74217 Purine salvage
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-74217 Purine salvage
Reactome:R-RNO-74217 Purine salvage
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-15869 Nucleotide metabolism
Reactome:R-DDI-15869 Nucleotide metabolism
Reactome:R-HSA-8956321 Nucleotide salvage
Reactome:R-MMU-8956321 Nucleotide salvage
Reactome:R-RNO-8956321 Nucleotide salvage
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-74217 Purine salvage
Reactome:R-DDI-74217 Purine salvage
Reactome:R-RNO-1430728 Metabolism
Reactome:R-SCE-15869 Nucleotide metabolism
Reactome:R-SPO-8956321 Nucleotide salvage
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-RNO-15869 Nucleotide metabolism
Reactome:R-SCE-8956321 Nucleotide salvage
Reactome:R-SPO-74217 Purine salvage
WikiPathways:WP4792 Purine metabolism
WikiPathways:WP4224 Purine metabolism and related disorders
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0012052 AMP Degradation (Hypoxanthine Route)
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000427 Azathioprine Action Pathway