EC: 3.4.11.18

methionyl aminopeptidase ()

enzyme lambda metabolic reaction experiment
uniprot:Q6UB28 [methionyl aminopeptidase(Enzyme) wildtype MetAP1D /deltaTS]

H2O + Met_Pro_p_nitroanilide -> Prolyl_p_nitroanilide + L_Methionine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM HEPES, 100 mM NaCl, 1 U/ml Proline aminopeptidase
PH: 8
Temperature: 23
uniprot:Q6UB28 [methionyl aminopeptidase(Enzyme) wildtype MetAP1D(His-tagged) /deltaTS]

Met_Pro_p_nitroanilide + H2O -> Prolyl_p_nitroanilide + L_Methionine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM HEPES, 100 mM NaCl, 1 U/ml Proline aminopeptidase
PH: 8
Temperature: 23
uniprot:Q6UB28 [methionyl aminopeptidase(Enzyme) wildtype MetAP1D]

H2O + Met_Pro_p_nitroanilide -> Prolyl_p_nitroanilide + L_Methionine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM HEPES, 100 mM NaCl, 1 U/ml Proline aminopeptidase
PH: 8
Temperature: 23
uniprot:P0AE20 [methionyl aminopeptidase(Enzyme) wildtype MetAP]

Met_Pro_p_nitroanilide + H2O -> Prolyl_p_nitroanilide + L_Methionine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM HEPES, 100 mM NaCl, 1 U/ml Proline aminopeptidase
PH: 8
Temperature: 23
uniprot:P53582 [methionyl aminopeptidase(Enzyme) mutant MetAP1 H212K]

Met_Pro_p_nitroanilide + H2O -> L_Methionine + Prolyl_p_nitroanilide		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM HEPES, 100 mM NaCl, 1 U/ml Proline aminopeptidase
PH: 8
Temperature: 23
uniprot:P53582 [methionyl aminopeptidase(Enzyme) mutant MetAP1 H212A]

Met_Pro_p_nitroanilide + H2O -> Prolyl_p_nitroanilide + L_Methionine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM HEPES, 100 mM NaCl, 1 U/ml Proline aminopeptidase
PH: 8
Temperature: 23
uniprot:P53582 [methionyl aminopeptidase(Enzyme) wildtype MetAP1]

Met_Pro_p_nitroanilide + H2O -> Prolyl_p_nitroanilide + L_Methionine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM HEPES, 100 mM NaCl, 1 U/ml Proline aminopeptidase
PH: 8
Temperature: 23
uniprot:Q6UB28 [Co2+]

Met_Pro_p_nitroanilide + H2O -> L_Methionine + Prolyl_p_nitroanilide		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 40 mM HEPES, 100 mM NaCl, 1 U/ml Proline aminopeptidase
PH: 8
Temperature: 23
uniprot:Q6UB28 [Co2+]

H2O + Met_Pro_p_nitroanilide -> Prolyl_p_nitroanilide + L_Methionine		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 40 mM HEPES, 100 mM NaCl, 1 U/ml Proline aminopeptidase
PH: 8
Temperature: 23
uniprot:P53582 [Co2+]

H2O + Met_Pro_p_nitroanilide -> L_Methionine + Prolyl_p_nitroanilide		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 40 mM HEPES, 100 mM NaCl, 1 U/ml Proline aminopeptidase
PH: 8
Temperature: 23
uniprot:P0AE20 [Co2+]

Met_Pro_p_nitroanilide + H2O -> L_Methionine + Prolyl_p_nitroanilide		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 40 mM HEPES, 100 mM NaCl, 1 U/ml Proline aminopeptidase
PH: 8
Temperature: 23
uniprot:P53582 [Co2+]

Met_Pro_p_nitroanilide + H2O -> L_Methionine + Prolyl_p_nitroanilide		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 40 mM HEPES, 100 mM NaCl, 1 U/ml Proline aminopeptidase
PH: 8
Temperature: 23
uniprot:P53582 [Co2+]

Met_Pro_p_nitroanilide + H2O -> L_Methionine + Prolyl_p_nitroanilide		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 40 mM HEPES, 100 mM NaCl, 1 U/ml Proline aminopeptidase
PH: 8
Temperature: 23
uniprot:P0AE18 [Co2+]

Met_Gly_Met_Met + H2O -> Gly_Met_Met + L_Methionine		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM HEPES, 150 mM KCl
PH: 7.5
Temperature: 30
uniprot:P0AE18 [Co2+]

H2O + Met_Gly_Met_Met -> L_Methionine + Gly_Met_Met		 (( (( (( (kcat_max / (( (1 + (H / (10 ^ (0 - pK_ES1)))) ) + (( (10 ^ (0 - pK_ES2)) ) / H))) ) * E) ) * S) ) / (( (( (kcat_max / (( (1 + (H / (10 ^ (0 - pK_ES1)))) ) + (( (10 ^ (0 - pK_ES2)) ) / H))) ) / (kcatKm_max / (( (1 + (H / (10 ^ (0 - pK_E1)))) ) + (( (10 ^ (0 - pK_E2)) ) / H)))) ) + S)) buffer: 30 mM MES (pH 5.5-6.0) / PIPES (pH 6.5-6.75) / HEPES (pH 7.0-8.0) / EPPS (pH 8.0-8.5), 150 mM KCl
PH: 5.5
Temperature: 30
uniprot:P0AE18 [Fe2+]

H2O + Met_Pro_p_nitroanilide -> Prolyl_p_nitroanilide + L_Methionine		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM HEPES, 150 mM KCl
PH: 7.5
Temperature: 30
uniprot:P0AE18 [Fe2+]

H2O + Met_Pro_p_nitroanilide -> Prolyl_p_nitroanilide + L_Methionine		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM HEPES, 150 mM KCl
PH: 7.5
Temperature: 30
uniprot:P0AE18 [Fe2+]

H2O + Met_Gly_Met_Met -> L_Methionine + Gly_Met_Met		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM HEPES, 150 mM KCl
PH: 7.5
Temperature: 30
uniprot:P0AE18 [Fe2+]

H2O + Met_Gly_Met_Met -> L_Methionine + Gly_Met_Met		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM HEPES, 150 mM KCl
PH: 7.5
Temperature: 30
uniprot:P0AE18 [Co2+]

H2O + Met_Pro_p_nitroanilide -> L_Methionine + Prolyl_p_nitroanilide		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM HEPES, 150 mM KCl
PH: 7.5
Temperature: 30
uniprot:P0AE18 [Co2+]

H2O + Met_Pro_p_nitroanilide -> L_Methionine + Prolyl_p_nitroanilide		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM HEPES, 150 mM KCl
PH: 7.5
Temperature: 30
uniprot:P0AE18 [Co2+]

Met_Gly_Met_Met + H2O -> L_Methionine + Gly_Met_Met		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM HEPES, 150 mM KCl
PH: 7.5
Temperature: 30
uniprot:Q8SR45 [Co2+]

Met_Ala_Ser + H2O -> L_Methionine + Ala_Ser		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 100 µg/ml BSA, 2% glycerol, 0.0004 U/µl L-amino acid oxidase, 0.01 U/µl horseradish peroxidase, 1 mM o-dianisidine
PH: 8.5
Temperature: 37
uniprot:P50579 [Co2+]

Met_Ala_Ser + H2O -> Ala_Ser + L_Methionine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 100 mM NaCl, 100 µg/ml BSA, 2% glycerol, 0.0004 U/µl L-amino acid oxidase, 0.01 U/µl horseradish peroxidase, 1 mM o-dianisidine
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-7800 Ac/N-end rule pathway
BioCyc:HUMAN_PWY-7799 N-end rule pathway I (eukaryotic)
BioCyc:META_PWY-7799 Arg/N-end rule pathway (eukaryotic)
BioCyc:YEAST_PWY-7800 Ac/N-end rule pathway
WikiPathways:WP2472 Escherichia coli K-12 peripherome
Reactome:R-CEL-9709957 Sensory Perception
Reactome:R-XTR-9709957 Sensory Perception
Reactome:R-GGA-9709957 Sensory Perception
Reactome:R-BTA-9709957 Sensory Perception
Reactome:R-HSA-9709957 Sensory Perception
Reactome:R-SPO-9709957 Sensory Perception
Reactome:R-MMU-9709957 Sensory Perception
Reactome:R-DRE-9709957 Sensory Perception
Reactome:R-RNO-9709957 Sensory Perception
Reactome:R-DDI-9709957 Sensory Perception
Reactome:R-SCE-9709957 Sensory Perception
Reactome:R-SPO-2514856 The phototransduction cascade
Reactome:R-SPO-2514859 Inactivation, recovery and regulation of the phototransduction cascade
Reactome:R-BTA-2514859 Inactivation, recovery and regulation of the phototransduction cascade
Reactome:R-CEL-2514859 Inactivation, recovery and regulation of the phototransduction cascade
Reactome:R-DDI-2187338 Visual phototransduction
Reactome:R-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade
Reactome:R-RNO-2514856 The phototransduction cascade
Reactome:R-SCE-2514856 The phototransduction cascade
Reactome:R-XTR-2514859 Inactivation, recovery and regulation of the phototransduction cascade
Reactome:R-BTA-2187338 Visual phototransduction
Reactome:R-CEL-2187338 Visual phototransduction
Reactome:R-DRE-2514859 Inactivation, recovery and regulation of the phototransduction cascade
Reactome:R-GGA-2514859 Inactivation, recovery and regulation of the phototransduction cascade
Reactome:R-HSA-2514859 Inactivation, recovery and regulation of the phototransduction cascade
Reactome:R-MMU-2187338 Visual phototransduction
Reactome:R-XTR-2187338 Visual phototransduction
Reactome:R-DRE-2187338 Visual phototransduction
Reactome:R-DRE-2514856 The phototransduction cascade
Reactome:R-DDI-2514856 The phototransduction cascade
Reactome:R-GGA-2514856 The phototransduction cascade
Reactome:R-HSA-2187338 Visual phototransduction
Reactome:R-HSA-2514856 The phototransduction cascade
Reactome:R-RNO-2187338 Visual phototransduction
Reactome:R-SCE-2187338 Visual phototransduction
Reactome:R-SCE-2514859 Inactivation, recovery and regulation of the phototransduction cascade
Reactome:R-BTA-2514856 The phototransduction cascade
Reactome:R-CEL-2514856 The phototransduction cascade
Reactome:R-DDI-2514859 Inactivation, recovery and regulation of the phototransduction cascade
Reactome:R-GGA-2187338 Visual phototransduction
Reactome:R-MMU-2514856 The phototransduction cascade
Reactome:R-RNO-2514859 Inactivation, recovery and regulation of the phototransduction cascade
Reactome:R-SPO-2187338 Visual phototransduction
Reactome:R-XTR-2514856 The phototransduction cascade