EC: 3.2.1.22

alpha-galactosidase (alpha-D-galactoside galactohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:P06280 [alpha-galactosidase(Enzyme) wildtype isoenzyme A]

H2O + 4_Methylumbelliferyl_alpha_D_galactoside -> alpha_D_Galactose + 4_Methylumbelliferone		 (( (Vmax * A) ) / (Km + A)) buffer: 0.15 M Citrate/phosphate
PH: 4.4
Temperature: 20
uniprot:P17050 [alpha-galactosidase(Enzyme) wildtype isoenzyme B]

4_Methylumbelliferyl_alpha_D_galactoside + H2O -> 4_Methylumbelliferone + alpha_D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.15 M Citrate/phosphate
PH: 4.4
Temperature: 20
uniprot:P06280 [alpha-galactosidase(Enzyme) wildtype]

4_Methylumbelliferyl_alpha_D_galactoside + H2O -> alpha_D_Galactose + 4_Methylumbelliferone		 (( (Vmax * S) ) / (S + Km)) buffer: 0.017 M citrate phosphate
PH: 4.5
Temperature: 37
uniprot:P06280 [alpha-galactosidase(Enzyme) wildtype]

4_Methylumbelliferyl_alpha_D_galactoside + H2O -> alpha_D_Galactose + 4_Methylumbelliferone		 (( (Vmax * S) ) / (S + Km)) buffer: 0.017 M citrate phosphate
PH: 4.5
Temperature: 37
uniprot:P06280 [alpha-galactosidase(Enzyme) wildtype]

4_Methylumbelliferyl_alpha_D_galactoside + H2O -> alpha_D_Galactose + 4_Methylumbelliferone		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Sodium citrate, 0.7% Bovine serum albumin
PH: 4
Temperature: 37
uniprot:P06280 [alpha-galactosidase(Enzyme) wildtype]

H2O + Trihexosylceramide -> beta_D_Galactosyl_1,4_beta_D_glucosylceramide + alpha_D_Galactose		 (( (Vmax * S) ) / (S + Km)) buffer: 50 mM sodium acetate, 9.3 mM sodium taurocholate
PH: 3.8
Temperature: 37
uniprot:P06280 [alpha-galactosidase(Enzyme) wildtype]

H2O + 4_Methylumbelliferyl_alpha_D_galactoside -> 4_Methylumbelliferone + alpha_D_Galactose		 (( (Vmax * S) ) / (S + Km)) buffer: 39 mM Na2HPO4, 23 mM citric acid
PH: 4.6
Temperature: 37
uniprot:P06280 [alpha-galactosidase(Enzyme) wildtype]

H2O + 4_Methylumbelliferyl_alpha_D_galactoside -> alpha_D_Galactose + 4_Methylumbelliferone		 (( (Vmax * S) ) / (S + Km)) buffer: 39 mM Na2HPO4, 23 mM citric acid
PH: 4.6
Temperature: 37
uniprot:P06280 [alpha-galactosidase(Enzyme) wildtype]

4_Methylumbelliferyl_alpha_D_galactoside + H2O -> 4_Methylumbelliferone + alpha_D_Galactose		 (( (Vmax * S) ) / (S + Km)) buffer: 39 mM Na2HPO4, 23 mM citric acid
PH: 4.6
Temperature: 37
uniprot:Q8A6L0 [Ca2+]

H2O + p_Nitrophenyl_alpha_D_galactoside -> alpha_D_Galactose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Sodium phosphate, 0.01% BSA
PH: 6
Temperature: 35
uniprot:Q8A6L0 [Ca2+]

p_Nitrophenyl_alpha_D_galactoside + H2O -> p_Nitrophenol + alpha_D_Galactose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Sodium phosphate, 0.01% BSA, 2 M NaN3
PH: 6
Temperature: 35
uniprot:Q8A6L0 [Ca2+]

p_Nitrophenyl_alpha_D_galactoside + H2O -> alpha_D_Galactose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Sodium phosphate, 0.01% BSA
PH: 6
Temperature: 35
uniprot:Q8A6L0 [alpha-galactosidase(Enzyme) wildtype His-tagged]

p_Nitrophenyl_alpha_D_galactoside + H2O -> alpha_D_Galactose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 40 mM Sodium phosphate, 0.01% BSA
PH: 6
Temperature: 35
uniprot:Q8A6L0 [Ca2+]

Melibiose + H2O -> D_Glucose + D_Galactose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM sodium maleate, 1 mg/ml BSA
PH: 6.6
Temperature: 37
uniprot:Q8A6L0 [Ca2+]

p_Nitrophenyl_alpha_D_galactoside + H2O -> alpha_D_Galactose + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM maleate, 1 mg/ml BSA
PH: 6.6
Temperature: 37
uniprot:P51569 [alpha-galactosidase(Enzyme) wildtype]

H2O + 4_Methylumbelliferyl_alpha_D_galactoside -> alpha_D_Galactose + 4_Methylumbelliferone		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Sodium citrate, 0.7 % BSA,
PH: 4.3
Temperature: 37
uniprot:P06280 [alpha-galactosidase(Enzyme) wildtype incubated with neuraminidase]

4_Methylumbelliferyl_alpha_D_galactoside + H2O -> alpha_D_Galactose + 4_Methylumbelliferone		 (( (Vmax * S) ) / (Km + S)) buffer: 154 mM acetate
PH: 5
Temperature: 37
uniprot:P06280 [alpha-galactosidase(Enzyme) wildtype]

H2O + 4_Methylumbelliferyl_alpha_D_galactoside -> 4_Methylumbelliferone + alpha_D_Galactose		 (( (Vmax * S) ) / (Km + S)) buffer: 154 mM acetate
PH: 5
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase I]

H2O + Raffinose -> Sucrose + D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Na2HPO4, 20 mM Citric acid
PH: 4.2
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase II]

Raffinose + H2O -> D_Galactose + Sucrose		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Na2HPO4, 20 mM Citric acid
PH: 4.2
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase II]

H2O + Melibiose -> D_Galactose + D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Na2HPO4, 20 mM Citric acid
PH: 4.2
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase I]

H2O + Melibiose -> D_Galactose + D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Na2HPO4, 20 mM Citric acid
PH: 4.2
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase II]

H2O + Stachyose -> Raffinose + D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Na2HPO4, 20 mM Citric acid
PH: 4.2
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase I]

Stachyose + H2O -> Raffinose + D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Na2HPO4, 20 mM Citric acid
PH: 4.2
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase II]

H2O + o_Nitrophenyl_alpha_D_galactoside -> alpha_D_Galactose + o_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Na2HPO4, 20 mM Citric acid
PH: 4.2
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase I]

H2O + o_Nitrophenyl_alpha_D_galactoside -> o_Nitrophenol + alpha_D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM Na2HPO4, 20 mM Citric acid
PH: 4.2
Temperature: 37
uniprot:Q5L7M8 [alpha-galactosidase(Enzyme) wildtype BfGal110A]

H2O + p_Nitrophenyl_alpha_D_galactoside -> alpha_D_Galactose + p_Nitrophenol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM NaPO4, 50 mM NaCl, 0.1-0.2 mg/ml Bovine serum albumin
PH: 6.8
Temperature: 26
uniprot:Q5LGZ8 [alpha-galactosidase(Enzyme) wildtype BfGal110B]

H2O + p_Nitrophenyl_alpha_D_galactoside -> p_Nitrophenol + alpha_D_Galactose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM NaPO4, 50 mM NaCl, 0.1-0.2 mg/ml Bovine serum albumin
PH: 6.8
Temperature: 26
- [alpha-galactosidase(Enzyme) wildtype]

Raffinose + H2O -> D_Galactose + Sucrose		 (( (Vmax * A) ) / (Km + A)) buffer: 16.7 mM Sodium acetate
PH: 5
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_alpha_D_galactoside -> alpha_D_Galactose + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 16.7 mM Sodium acetate
PH: 5
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype]

Raffinose + H2O -> D_Galactose + Sucrose		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Citrate-sodium phosphate
PH: 5.8
Temperature: 30
- [alpha-galactosidase(Enzyme) wildtype]

Melibiose + H2O -> D_Glucose + D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Citrate-sodium phosphate
PH: 5.8
Temperature: 30
- [alpha-galactosidase(Enzyme) wildtype]

Stachyose + H2O -> D_Galactose + Raffinose		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Citrate-sodium phosphate
PH: 5.8
Temperature: 30
- [alpha-galactosidase(Enzyme) wildtype]

H2O + Raffinose -> Sucrose + D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Citrate-sodium phosphate
PH: 5
Temperature: 30
- [alpha-galactosidase(Enzyme) wildtype]

Melibiose + H2O -> D_Galactose + D_Glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Citrate-sodium phosphate
PH: 5
Temperature: 30
- [alpha-galactosidase(Enzyme) wildtype]

H2O + Stachyose -> Raffinose + D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Citrate-sodium phosphate
PH: 5
Temperature: 30
- [alpha-galactosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_galactoside + H2O -> alpha_D_Galactose + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Citrate-sodium phosphate
PH: 5.8
Temperature: 30
- [alpha-galactosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_galactoside + H2O -> p_Nitrophenol + alpha_D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Citrate-sodium phosphate
PH: 5
Temperature: 30
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase II]

Raffinose + H2O -> D_Galactose + Sucrose		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Potassium phosphate
PH: 6.3
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase I]

Raffinose + H2O -> D_Galactose + Sucrose		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Potassium phosphate
PH: 6.3
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase II]

Melibiose + H2O -> D_Glucose + D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Potassium phosphate
PH: 6.3
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase I]

H2O + Melibiose -> D_Glucose + D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Potassium phosphate
PH: 6.3
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase II]

H2O + Stachyose -> Raffinose + D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Potassium phosphate
PH: 6.3
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase I]

H2O + Stachyose -> D_Galactose + Raffinose		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Potassium phosphate
PH: 6.3
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase II]

p_Nitrophenyl_beta_D_galactoside + H2O -> beta_D_Galactose + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Potassium phosphate
PH: 6.3
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_galactoside + H2O -> p_Nitrophenol + alpha_D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Na2HPO4, 100 mM Citric acid
PH: 7.5
Temperature: 30
- [alpha-galactosidase(Enzyme) wildtype]

o_Nitrophenyl_alpha_D_galactoside + H2O -> alpha_D_Galactose + o_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Na2HPO4, 100 mM Citric acid
PH: 7.5
Temperature: 30
- [alpha-galactosidase(Enzyme) wildtype]

m_Nitrophenyl_alpha_D_galactoside + H2O -> alpha_D_Galactose + 3_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Na2HPO4, 100 mM Citric acid
PH: 7.5
Temperature: 30
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase I]

H2O + p_Nitrophenyl_beta_D_galactoside -> p_Nitrophenol + beta_D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Potassium phosphate
PH: 6.3
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase I]

H2O + o_Nitrophenyl_beta_D_galactoside -> beta_D_Galactose + o_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Potassium phosphate
PH: 6.3
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype alpha-galactosidase II]

H2O + o_Nitrophenyl_beta_D_galactoside -> o_Nitrophenol + beta_D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Potassium phosphate
PH: 6.3
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_alpha_D_galactoside -> alpha_D_Galactose + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Sodium phosphate
PH: 6
Temperature: 40
- [alpha-galactosidase(Enzyme) wildtype]

H2O + o_Nitrophenyl_alpha_D_galactoside -> alpha_D_Galactose + o_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Sodium phosphate
PH: 6
Temperature: 40
- [alpha-galactosidase(Enzyme) wildtype]

m_Nitrophenyl_alpha_D_galactoside + H2O -> alpha_D_Galactose + 3_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Sodium phosphate
PH: 6
Temperature: 40
- [alpha-galactosidase(Enzyme) wildtype]

Raffinose + H2O -> Sucrose + D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Na2HPO4, 100 mM Citric acid
PH: 7.5
Temperature: 30
- [alpha-galactosidase(Enzyme) wildtype]

H2O + Stachyose -> Raffinose + D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Na2HPO4, 100 mM Citric acid
PH: 7.5
Temperature: 30
- [alpha-galactosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_galactoside + H2O -> alpha_D_Galactose + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_galactoside + H2O -> p_Nitrophenol + alpha_D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_galactoside + H2O -> p_Nitrophenol + alpha_D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_galactoside + H2O -> alpha_D_Galactose + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_alpha_D_galactoside -> p_Nitrophenol + alpha_D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_galactoside + H2O -> p_Nitrophenol + alpha_D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_galactoside + H2O -> alpha_D_Galactose + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_alpha_D_galactoside -> p_Nitrophenol + alpha_D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_alpha_D_galactoside -> p_Nitrophenol + alpha_D_Galactose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37
- [alpha-galactosidase(Enzyme) wildtype]

p_Nitrophenyl_alpha_D_galactoside + H2O -> alpha_D_Galactose + p_Nitrophenol		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium citrate/citric acid
PH: 4.5
Temperature: 37

Pathways

pathway id name
BioCyc:ARA_PWY-5343 ajugose biosynthesis II (galactinol-independent)
BioCyc:BSUB_PWY0-1301 melibiose degradation
BioCyc:ECOL316407_PWY0-1301 melibiose degradation
BioCyc:META_PWY0-1301 melibiose degradation
BioCyc:ECO_PWY0-1301 melibiose degradation
BioCyc:MOUSE_PWY0-1301 melibiose degradation
BioCyc:BTHE_GLYCOCAT-PWY glycogen degradation I
PathBank:SMP0000841 Galactose Metabolism
Reactome:R-HSA-9840310 Glycosphingolipid catabolism
Reactome:R-MMU-9840310 Glycosphingolipid catabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-428157 Sphingolipid metabolism
Reactome:R-MMU-1660662 Glycosphingolipid metabolism
Reactome:R-HSA-1660662 Glycosphingolipid metabolism
Reactome:R-MMU-428157 Sphingolipid metabolism
Reactome:R-HSA-1430728 Metabolism
PathBank:SMP0000349 Gaucher Disease
PathBank:SMP0000043 Galactose Metabolism
PathBank:SMP0000182 Galactosemia
PathBank:SMP0000348 Globoid Cell Leukodystrophy
PathBank:SMP0000525 Fabry Disease
PathBank:SMP0000034 Sphingolipid Metabolism
PathBank:SMP0000347 Metachromatic Leukodystrophy (MLD)
PathBank:SMP0000526 Krabbe Disease