EC: 3.2.1.114

mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase ((1->3)-(1->6)-mannosyl-oligosaccharide alpha-D-mannohydrolase (configuration-retaining))

enzyme lambda metabolic reaction experiment
uniprot:Q24451 [mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase(Enzyme) mutant D341N]

2,4_Dinitrophenyl_alpha_D_mannoside + H2O -> 2,4_Dinitrophenol + alpha_D_Mannose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM 4-morpholineethanesulfonic acid, 0.1 mM ZnSO4, 0.1% BSA
PH: 5.6
Temperature: 37
uniprot:Q24451 [mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase(Enzyme) wildtype]

2,4_Dinitrophenyl_alpha_D_mannoside + H2O -> alpha_D_Mannose + 2,4_Dinitrophenol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM 4-morpholineethanesulfonic acid, 0.1 mM ZnSO4, 0.1% BSA
PH: 5.6
Temperature: 37
uniprot:Q24451 [mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase(Enzyme) wildtype]

H2O + 5_Fluoro_beta_L_gulosyl_fluoride -> Unknown		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM 4-morpholineethanesulfonic acid, 0.1 mM ZnSO4, 0.1% BSA
PH: 5.6
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-7426 complex N-linked glycan biosynthesis (vertebrates)
BioCyc:META_PWY-7920 complex N-linked glycan biosynthesis (plants)
BioCyc:HUMAN_PWY-7426 complex N-linked glycan biosynthesis (vertebrates)
Reactome:R-HSA-9679506 SARS-CoV Infections
Reactome:R-HSA-9694516 SARS-CoV-2 Infection
Reactome:R-HSA-9694635 Translation of Structural Proteins
Reactome:R-HSA-9772573 Late SARS-CoV-2 Infection Events
Reactome:R-HSA-9824446 Viral Infection Pathways
Reactome:R-HSA-9694548 Maturation of spike protein
Reactome:R-DME-597592 Post-translational protein modification
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-HSA-975576 N-glycan antennae elongation in the medial/trans-Golgi
Reactome:R-HSA-1643685 Disease
Reactome:R-RNO-597592 Post-translational protein modification
Reactome:R-RNO-975576 N-glycan antennae elongation in the medial/trans-Golgi
Reactome:R-DME-975578 Reactions specific to the complex N-glycan synthesis pathway
Reactome:R-MMU-975578 Reactions specific to the complex N-glycan synthesis pathway
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-DME-392499 Metabolism of proteins
Reactome:R-DME-975576 N-glycan antennae elongation in the medial/trans-Golgi
Reactome:R-HSA-446203 Asparagine N-linked glycosylation
Reactome:R-HSA-948021 Transport to the Golgi and subsequent modification
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-MMU-975576 N-glycan antennae elongation in the medial/trans-Golgi
Reactome:R-RNO-446203 Asparagine N-linked glycosylation
Reactome:R-RNO-948021 Transport to the Golgi and subsequent modification
Reactome:R-DME-446203 Asparagine N-linked glycosylation
Reactome:R-DME-948021 Transport to the Golgi and subsequent modification
Reactome:R-HSA-975578 Reactions specific to the complex N-glycan synthesis pathway
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-MMU-446203 Asparagine N-linked glycosylation
Reactome:R-MMU-948021 Transport to the Golgi and subsequent modification
Reactome:R-RNO-975578 Reactions specific to the complex N-glycan synthesis pathway
WikiPathways:WP5021 SARS-CoV-2 Infection