N-glycan antennae elongation in the medial/trans-Golgi

Ito H, Chiba Y, Kameyama A, Sato T, Narimatsu H. In vitro and in vivo enzymatic syntheses and mass spectrometric database for N-glycans and o-glycans. Methods Enzymol. 2010;478():127–49. doi: 10.1016/s0076-6879(10)78005-8. PMID: 20816477.; Hossler P, Goh LT, Lee MM, Hu WS. GlycoVis: visualizing glycan distribution in the protein N-glycosylation pathway in mammalian cells. Biotechnol Bioeng. 2006 Dec 05;95(5):946–60. doi: 10.1002/bit.21062. PMID: 16807922.; Schachter H. The joys of HexNAc. The synthesis and function of N- and O-glycan branches. Glycoconj J. 2000 Jul;17(7-9):465–83. doi: 10.1023/a:1011010206774. PMID: 11421343.; Kornfeld R, Kornfeld S. Assembly of asparagine-linked oligosaccharides. Annu Rev Biochem. 1985;54():631–64. doi: 10.1146/annurev.bi.54.070185.003215. PMID: 3896128.

Metabolites

D-Mannose

Formula: C6H12O6 (180.0633852)

CAS ID: 3458-28-4

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Manganese(2+)

Formula: Mn (54.938046)

CAS ID: 7439-96-5

N-Acetylneuraminate

Formula: C11H19NO9 (309.10597640000003)

CAS ID: 131-48-6

Zinc cation

Formula: Zn (63.929145)

CAS ID: 23713-49-7

UDP(3-)

Formula: C9H11N2O12P2 (400.9787246)

CAS ID: 86527-70-0



Enzyme

EC Number name full name note
2.4.1.-
2.4.1.143 alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase UDP-N-acetyl-alpha-D-glucosamine:alpha-D-mannosyl-(1->6)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
2.4.1.144 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase UDP-N-acetyl-alpha-D-glucosamine:beta-D-mannosyl-glycoprotein 4-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
2.4.1.152 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase GDP-beta-L-fucose:beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R 3-alpha-L-fucosyltransferase (configuration-inverting)
2.4.1.155 alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase UDP-N-acetyl-alpha-D-glucosamine:N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)-beta-D-mannosyl-glycoprotein 6-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
2.4.1.22 lactose synthase UDP-galactose:D-glucose 4-beta-D-galactosyltransferase
2.4.1.274 glucosylceramide beta-1,4-galactosyltransferase UDP-galactose:beta-D-glucosyl-(1<->1)-ceramide beta-1,4-galactosyltransferase
2.4.1.275 neolactotriaosylceramide beta-1,4-galactosyltransferase UDP-alpha-D-galactose:N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1beta4)-beta-D-glucosyl-(1<->1)-ceramide 4-beta-D-galactosyltransferase
2.4.1.38 beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase UDP-galactose:N-acetyl-beta-D-glucosaminylglycopeptide 4-beta-galactosyltransferase
2.4.1.65 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase GDP-beta-L-fucose:beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R 4I-alpha-L-fucosyltransferase
2.4.1.68 glycoprotein 6-alpha-L-fucosyltransferase GDP-beta-L-fucose:glycoprotein (L-fucose to asparagine-linked N-acetylglucosamine of N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl}asparagine) 6-alpha-L-fucosyltransferase
2.4.1.90 N-acetyllactosamine synthase UDP-galactose:N-acetyl-D-glucosamine 4-beta-D-galactosyltransferase
2.4.3.1 beta-galactoside alpha-(2,6)-sialyltransferase CMP-N-acetyl-beta-neuraminate:beta-D-galactoside alpha-(2->6)-N-acetylneuraminyltransferase (configuration-inverting)
2.4.3.2 beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminide alpha-2,3-sialyltransferase CMP-N-acetyl-beta-neuraminate:a beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R alpha-(2->3)-N-acetylneuraminyltransferase (configuration-inverting)
2.4.3.4 beta-galactoside alpha-2,3-sialyltransferase CMP-N-acetylneuraminate:beta-D-galactoside alpha-(2->3)-N-acetylneuraminyl-transferase
2.4.3.6 N-acetyllactosaminide alpha-2,3-sialyltransferase CMP-N-acetyl-beta-neuraminate:beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R (2->3)-N-acetyl-alpha-neuraminyltransferase (configuration-inverting)
3.2.1.114 mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase (1->3)-(1->6)-mannosyl-oligosaccharide alpha-D-mannohydrolase (configuration-retaining)
3.2.1.51 alpha-L-fucosidase alpha-L-fucoside fucohydrolase


Proteins

Protein ID name full name