EC: 3.1.3.74

pyridoxal phosphatase (pyridoxal-5'-phosphate phosphohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q96GD0 [pyridoxal phosphatase(Enzyme) wildtype His6-tagged]

Pyridoxal_phosphate + H2O -> Phosphate + Pyridoxal		 (( (Vmax * S) ) / (S + Km)) buffer: 40 mM triethanolamine-HCl, 4 mM MgCl2
PH: 7.4
Temperature: 37
uniprot:Q96GD0 [pyridoxal phosphatase(Enzyme) wildtype His6-tagged]

H2O + 4_Nitrophenyl_phosphate -> Phosphate + p_Nitrophenol		 (( (Vmax * S) ) / (S + Km)) buffer: 40 mM triethanolamine-HCl, 4 mM MgCl2
PH: 7.4
Temperature: 37
uniprot:Q96GD0 [pyridoxal phosphatase(Enzyme) wildtype His6-tagged]

Pyridoxine_phosphate + H2O -> Phosphate + Pyridoxine		 (( (Vmax * S) ) / (S + Km)) buffer: 40 mM triethanolamine-HCl, 4 mM MgCl2
PH: 7.4
Temperature: 37
uniprot:Q96GD0 [pyridoxal phosphatase(Enzyme) wildtype His6-tagged]

Pyridoxamine_phosphate + H2O -> Pyridoxamine + Phosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 40 mM triethanolamine-HCl, 4 mM MgCl2
PH: 7.4
Temperature: 37
- [pyridoxal phosphatase(Enzyme) wildtype]

H2O + Pyridoxal_phosphate -> Pyridoxal + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 37 mM Triethanolamine- HCl, 4 mM MgCl2, 0.001 % Triton X-100, 20 µg Ovalbumin
PH: 7.4
Temperature: 37
- [pyridoxal phosphatase(Enzyme) wildtype]

Pyridoxine_phosphate + H2O -> Pyridoxine + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 37 mM Triethanolamine- HCl, 4 mM MgCl2, 0.001 % Triton X-100, 20 µg Ovalbumin
PH: 7.4
Temperature: 37
- [pyridoxal phosphatase(Enzyme) wildtype]

Pyridoxamine_phosphate + H2O -> Phosphate + Pyridoxamine		 (( (Vmax * S) ) / (Km + S)) buffer: 37 mM Triethanolamine- HCl, 4 mM MgCl2, 0.001 % Triton X-100, 20 µg Ovalbumin
PH: 7.4
Temperature: 37
- [pyridoxal phosphatase(Enzyme) wildtype]

4_Pyridoxic_acid_5_phosphate + H2O -> 4_Pyridoxate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 37 mM Triethanolamine- HCl, 4 mM MgCl2, 0.001 % Triton X-100, 20 µg Ovalbumin
PH: 7.4
Temperature: 37
uniprot:Q96GD0 [Ethylenediaminetetraacetic acid]

Pyridoxine_phosphate + H2O -> Phosphate + Pyridoxine		 (( (Vmax * A) ) / (Km + A)) buffer: 37 mM triethanolamine-HCl
PH: 7.4
Temperature: 37
uniprot:Q96GD0 [Mg2+]

Pyridoxine_phosphate + H2O -> Phosphate + Pyridoxine		 (( (Vmax * S) ) / (S + (Km * (1 + (P / Ki))))) buffer: 37 mM triethanolamine-HCl
PH: 7.4
Temperature: 37
uniprot:Q96GD0 [Mg2+]

Pyridoxal_phosphate + H2O -> Pyridoxal + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 37 mM triethanolamine-HCl
PH: 7.4
Temperature: 37
uniprot:Q96GD0 [Mg2+]

Pyridoxine_phosphate + H2O -> Pyridoxine + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 37 mM triethanolamine-HCl
PH: 7.4
Temperature: 37
uniprot:Q96GD0 [Mg2+]

H2O + Pyridoxamine_phosphate -> Pyridoxamine + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 37 mM triethanolamine-HCl
PH: 7.4
Temperature: 37
uniprot:Q96GD0 [Mg2+]

4_Pyridoxic_acid_5_phosphate + H2O -> 4_Pyridoxate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 37 mM triethanolamine-HCl
PH: 7.4
Temperature: 37

Pathways

pathway id name
BioCyc:META_P441-PWY superpathway of N-acetylneuraminate degradation
BioCyc:ECO_GLUCONEO-PWY gluconeogenesis I
BioCyc:ECOL316407_GLYCOLYSIS glycolysis I (from glucose-6P)
BioCyc:ECOL316407_GLYCOLYSIS-E-D superpathway of glycolysis and Entner-Doudoroff
BioCyc:ECOL316407_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:META_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:META_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:ECO_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:ECO_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:ECOL316407_GLUCONEO-PWY gluconeogenesis I
BioCyc:META_PWY-7218 photosynthetic 3-hydroxybutanoate biosynthesis (engineered)
BioCyc:META_GLUCONEO-PWY gluconeogenesis I
BioCyc:ECO_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:ECOL316407_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:META_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:META_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:META_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:ECO_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:ECO_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:ECOL316407_PWY-5484 glycolysis II (from fructose-6P)
PathBank:SMP0000807 Glycolysis and Pyruvate Dehydrogenase
PathBank:SMP0000839 Gluconeogenesis from L-Malic Acid
PathBank:SMP0000931 Glycerol Metabolism
PathBank:SMP0000932 Glycerol Metabolism II
PathBank:SMP0000933 Glycerol Metabolism III (sn-Glycero-3-Phosphoethanolamine)
PathBank:SMP0000934 Glycerol Metabolism IV (Glycerophosphoglycerol)
PathBank:SMP0000935 Glycerol Metabolism V (Glycerophosphoserine)
PathBank:SMP0087322 Phospholipid Biosynthesis
PathBank:SMP0000017 Vitamin B6 Metabolism
PathBank:SMP0000503 Hypophosphatasia
PathBank:SMP0087228 Phospholipid Biosynthesis
PathBank:SMP0087333 Vitamin B6 Metabolism
PathBank:SMP0087240 Vitamin B6 Metabolism
PathBank:SMP0120787 Hypophosphatasia