EC: 3.1.3.7

3'(2'),5'-bisphosphate nucleotidase (adenosine-3'(2'),5'-bisphosphate 3'(2')-phosphohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q9Z0S1 [Mg2+]

1D_myo_Inositol_1,4_bisphosphate + H2O -> myo_Inositol_4_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM NaHEPES, 100 mM KCl, 1 mM EGTA, 0.4 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:Q9Z0S1 [Mg2+]

H2O + 1D_myo_Inositol_1,4_bisphosphate -> Phosphate + myo_Inositol_4_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM NaHEPES, 100 mM KCl, 1 mM EGTA, 0.4 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:Q9Z0S1 [Mg2+]

H2O + Adenosine_3',5'_bisphosphate -> Phosphate + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM NaHEPES, 100 mM KCl, 1 mM EGTA, 0.4 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:Q9Z0S1 [Mg2+]

H2O + 1D_myo_Inositol_1,3,4_trisphosphate -> D_myo_Inositol_3,4_bisphosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM NaHEPES, 100 mM KCl, 1 mM EGTA, 0.4 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:Q9Z0S1 [Mg2+]

Adenosine_3',5'_bisphosphate + H2O -> Phosphate + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM NaHEPES, 100 mM KCl, 1 mM EGTA, 0.4 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:Q9Z0S1 [Mg2+]

Adenosine_3',5'_bisphosphate + H2O -> AMP + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM NaHEPES, 100 mM KCl, 1 mM EGTA, 0.4 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:Q5SM25 [Mn2+]

Adenosine_3',5'_bisphosphate + H2O -> Phosphate + AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl
PH: 7.5
Temperature: 37
uniprot:P75144 [Mn2+]

H2O + Adenosine_3',5'_bisphosphate -> AMP + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl
PH: 7.5
Temperature: 37
uniprot:P22255 [3'(2'),5'-bisphosphate nucleotidase(Enzyme) wildtype His6-tag]

H2O + Adenosine_2',5'_bisphosphate -> Phosphate + AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 8
Temperature: 37
uniprot:P22255 [3'(2'),5'-bisphosphate nucleotidase(Enzyme) wildtype His6-tag]

Adenosine_3',5'_bisphosphate + H2O -> Phosphate + AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 8
Temperature: 37
uniprot:Q80V26 [3'(2'),5'-bisphosphate nucleotidase(Enzyme) wildtype C-terminal His6-tag]

Adenosine_3',5'_bisphosphate + H2O -> Phosphate + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Bis-Tris, 50 µM MnCl2, 100 mM KCl, 0.1% Triton X-100, 0.1% phosphatidylcholine
PH: 6.5
Temperature: 37
uniprot:Q80V26 [3'(2'),5'-bisphosphate nucleotidase(Enzyme) mutant delta1-55, C-terminal His6-tag]

H2O + Adenosine_3',5'_bisphosphate -> Phosphate + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Bis-Tris, 10 mM Hepes (pH 6.5), 50 µM MnCl2, 0.6 mM MgCl2, 90 mM KCl, 80 ng/µl BSA
PH: 6.5
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-6358 superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism
BioCyc:META_PWY-2301 myo-inositol biosynthesis
BioCyc:ARA_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
BioCyc:MOUSE_PWY-2301 myo-inositol biosynthesis
BioCyc:META_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
BioCyc:MTBH37RV_GLUCONEO-PWY gluconeogenesis I
BioCyc:HUMAN_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
BioCyc:HUMAN_PWY-6358 superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism
BioCyc:MTBH37RV_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
PathBank:SMP0000939 Sulfur Metabolism
PathBank:SMP0000940 Sulfur Metabolism (Butanesulfonate)
PathBank:SMP0000941 Sulfur Metabolism (Propanesulfonate)
PathBank:SMP0000942 Sulfur Metabolism (Ethanesulfonate)
PathBank:SMP0000943 Sulfur Metabolism (Isethionate)
PathBank:SMP0000944 Sulfur Metabolism (Methanesulfonate)
PlantCyc:ARA_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
Reactome:R-BTA-1430728 Metabolism
Reactome:R-DDI-1483249 Inositol phosphate metabolism
Reactome:R-DDI-211859 Biological oxidations
Reactome:R-DDI-156584 Cytosolic sulfonation of small molecules
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-MTU-870392 Mycobacterium tuberculosis biological processes
Reactome:R-BTA-211859 Biological oxidations
Reactome:R-HSA-156580 Phase II - Conjugation of compounds
Reactome:R-MMU-156580 Phase II - Conjugation of compounds
Reactome:R-MTU-936635 Sulfate assimilation
Reactome:R-RNO-211859 Biological oxidations
Reactome:R-SSC-1430728 Metabolism
Reactome:R-SSC-211859 Biological oxidations
Reactome:R-BTA-156584 Cytosolic sulfonation of small molecules
Reactome:R-DDI-1855183 Synthesis of IP2, IP, and Ins in the cytosol
Reactome:R-DDI-156580 Phase II - Conjugation of compounds
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-156584 Cytosolic sulfonation of small molecules
Reactome:R-SSC-156584 Cytosolic sulfonation of small molecules
Reactome:R-BTA-156580 Phase II - Conjugation of compounds
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-156584 Cytosolic sulfonation of small molecules
Reactome:R-MMU-156584 Cytosolic sulfonation of small molecules
Reactome:R-MTU-936621 Sulfur compound metabolism
Reactome:R-RNO-156580 Phase II - Conjugation of compounds
Reactome:R-SSC-156580 Phase II - Conjugation of compounds
PathBank:SMP0120597 Sulfite Oxidase Deficiency
PathBank:SMP0000041 Sulfate/Sulfite Metabolism
PathBank:SMP0000532 Sulfite Oxidase Deficiency
PathBank:SMP0063690 Sulfate/Sulfite Metabolism
PathBank:SMP0087349 Inositol Phosphate Metabolism
PathBank:SMP0120816 Sulfite Oxidase Deficiency
PathBank:SMP0087311 Sulfate/Sulfite Metabolism
PathBank:SMP0087335 Inositol Metabolism