EC: 2.7.7.48

RNA-directed RNA polymerase (nucleoside-triphosphate:RNA nucleotidyltransferase (RNA-directed))

enzyme lambda metabolic reaction experiment
uniprot:P0C6X7 [RNA-directed RNA polymerase(Enzyme) wildtype complex (nsp8/nsp12) (aa 3920-4117/4370-5301), N-terminal His-tag]

ATP + RNA -> Diphosphate + RNA		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]GTP, 0.1 µM NTP, 5 mM MgCl2
PH: 8
Temperature: 30
uniprot:P0DTD1 [RNA-directed RNA polymerase(Enzyme) wildtype complex (nsp8/nsp12) (aa 3943-4140/4393-5324), N-terminal His-tag]

RNA + ATP -> RNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]GTP, 0.1 µM NTP, 5 mM MgCl2
PH: 8
Temperature: 30
uniprot:P0C6X7 [RNA-directed RNA polymerase(Enzyme) wildtype complex (nsp8/nsp12) (aa 3920-4117/4370-5301), N-terminal His-tag]

RNA + Remdesivir_triphosphate -> RNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]GTP, 0.1 µM NTP, 5 mM MgCl2
PH: 8
Temperature: 30
uniprot:P0DTD1 [RNA-directed RNA polymerase(Enzyme) wildtype complex (nsp8/nsp12) (aa 3943-4140/4393-5324), N-terminal His-tag]

RNA + Remdesivir_triphosphate -> Diphosphate + RNA		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]GTP, 0.1 µM NTP, 5 mM MgCl2
PH: 8
Temperature: 30
uniprot:P0DTD1 [RNA-directed RNA polymerase(Enzyme) wildtype complex (nsp8/nsp12) (aa 3943-4140/4393-5324), N-terminal His-tag]

RNA + ATP -> Diphosphate + RNA		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]CTP, 5 mM MgCl2
PH: 8
Temperature: 30
- [RNA-directed RNA polymerase(Enzyme) wildtype complex (L/VP35), N-terminal His-tag]

RNA + ATP -> RNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]GTP, 0.1 µM NTP, 5 mM MgCl2
PH: 8
Temperature: 30
uniprot:A0A097F4L1 [RNA-directed RNA polymerase(Enzyme) wildtype RdRp L, N-terminal Strep-tag]

ATP + RNA -> Diphosphate + RNA		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]GTP, 1 µM NTP, 5 mM MgCl2
PH: 8
Temperature: 30
uniprot:P0DTD1 [RNA-directed RNA polymerase(Enzyme) wildtype complex (nsp8/nsp12) (aa 3943-4140/4393-5324), N-terminal His-tag]

2'_C_Methyl_adenosine_triphosphate + RNA -> Diphosphate + RNA		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]GTP, 5 mM MgCl2
PH: 8
Temperature: 30
uniprot:P0DTD1 [RNA-directed RNA polymerase(Enzyme) wildtype complex (nsp8/nsp12) (aa 3943-4140/4393-5324), N-terminal His-tag]

RNA + Favipiravir_riboside_triphosphate -> RNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]CTP, 5 mM MgCl2
PH: 8
Temperature: 30
uniprot:P0DTD1 [RNA-directed RNA polymerase(Enzyme) wildtype complex (nsp8/nsp12) (aa 3943-4140/4393-5324), N-terminal His-tag]

dATP + RNA -> Diphosphate + RNA		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]GTP, 5 mM MgCl2
PH: 8
Temperature: 30
- [RNA-directed RNA polymerase(Enzyme) wildtype complex (L/VP35), N-terminal His-tag]

Remdesivir_triphosphate + RNA -> RNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]GTP, 0.1 µM NTP, 5 mM MgCl2
PH: 8
Temperature: 30
uniprot:A0A097F4L1 [RNA-directed RNA polymerase(Enzyme) wildtype RdRp L, N-terminal Strep-tag]

RNA + Remdesivir_triphosphate -> RNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]GTP, 1 µM NTP, 5 mM MgCl2
PH: 8
Temperature: 30
uniprot:P0DTD1 [RNA-directed RNA polymerase(Enzyme) wildtype complex (nsp8/nsp12) (aa 3943-4140/4393-5324), N-terminal His-tag]

ara_ATP + RNA -> RNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]GTP, 5 mM MgCl2
PH: 8
Temperature: 30
- [RNA-directed RNA polymerase(Enzyme) wildtype]

RNA + UTP -> RNA + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 40 mM Tris-HCl, 50 mM NaCl, 5 mM MgCl2, 1 mM DTT, 6 µM NSP8
PH: 7
Temperature: 37
uniprot:P0DTD1 [RNA-directed RNA polymerase(Enzyme) wildtype complex (nsp8/nsp12) (aa 3943-4140/4393-5324), N-terminal His-tag]

RNA + UTP -> Diphosphate + RNA		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]CTP, 5 mM MgCl2
PH: 8
Temperature: 30
uniprot:P0DTD1 [RNA-directed RNA polymerase(Enzyme) wildtype complex (nsp8/nsp12) (aa 3943-4140/4393-5324), N-terminal His-tag]

RNA + CTP -> Diphosphate + RNA		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]GTP, 5 mM MgCl2
PH: 8
Temperature: 30
uniprot:P0DTD1 [RNA-directed RNA polymerase(Enzyme) wildtype complex (nsp8/nsp12) (aa 3943-4140/4393-5324), N-terminal His-tag]

RNA + Sofosbuvir_triphosphate -> RNA + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]CTP, 5 mM MgCl2
PH: 8
Temperature: 30
uniprot:P0DTD1 [RNA-directed RNA polymerase(Enzyme) wildtype complex (nsp8/nsp12) (aa 3943-4140/4393-5324), N-terminal His-tag]

RNA + 2'_Fluoro_dCTP -> Diphosphate + RNA		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 200 µM RNA primer, 2 µM RNA template, 0.1 µM [alpha-32P]GTP, 5 mM MgCl2
PH: 8
Temperature: 30

Pathways

pathway id name
Reactome:R-HSA-9679506 SARS-CoV Infections
Reactome:R-HSA-9684325 Maturation of replicase proteins
Reactome:R-HSA-9682708 Transcription of SARS-CoV-1 sgRNAs
Reactome:R-HSA-9670095 Inhibition of DNA recombination at telomere
Reactome:R-HSA-9694516 SARS-CoV-2 Infection
Reactome:R-HSA-9694271 Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC)
Reactome:R-HSA-9772572 Early SARS-CoV-2 Infection Events
Reactome:R-HSA-9692914 SARS-CoV-1-host interactions
Reactome:R-HSA-9820965 Respiratory syncytial virus (RSV) genome replication, transcription and translation
Reactome:R-HSA-9828806 Maturation of hRSV A proteins
Reactome:R-HSA-9679504 Translation of Replicase and Assembly of the Replication Transcription Complex
Reactome:R-HSA-9682706 Replication of the SARS-CoV-1 genome
Reactome:R-HSA-9694301 Maturation of replicase proteins
Reactome:R-HSA-9694786 Transcription of SARS-CoV-2 sgRNAs
Reactome:R-HSA-9692916 SARS-CoV-1 activates/modulates innate immune responses
Reactome:R-HSA-9828721 Translation of respiratory syncytial virus mRNAs
Reactome:R-HSA-9678108 SARS-CoV-1 Infection
Reactome:R-HSA-9683439 Assembly of the SARS-CoV-1 Replication-Transcription Complex (RTC)
Reactome:R-HSA-9694682 SARS-CoV-2 Genome Replication and Transcription
Reactome:R-HSA-9824446 Viral Infection Pathways
Reactome:R-HSA-9679514 SARS-CoV-1 Genome Replication and Transcription
Reactome:R-HSA-9694676 Translation of Replicase and Assembly of the Replication Transcription Complex
Reactome:R-HSA-9820952 Respiratory Syncytial Virus Infection Pathway
Reactome:R-HSA-9918432 Maturation of DENV proteins
Reactome:R-HSA-9920951 Dengue virus modulates apoptosis
Reactome:R-HSA-9918487 Dengue Virus Genome Translation and Replication
Reactome:R-HSA-9920588 Dengue virus activates/modulates innate and adaptive immune responses
Reactome:R-HSA-9839923 Dengue Virus Infection
Reactome:R-HSA-9918476 Assembly and Release of Dengue Virus Virions
Reactome:R-HSA-9918481 Dengue Virus-Host Interactions
Reactome:R-BTA-74160 Gene expression (Transcription)
Reactome:R-BTA-73857 RNA Polymerase II Transcription
Reactome:R-BTA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
Reactome:R-BTA-5696398 Nucleotide Excision Repair
Reactome:R-BTA-6782135 Dual incision in TC-NER
Reactome:R-BTA-8939211 ESR-mediated signaling
Reactome:R-HSA-5696398 Nucleotide Excision Repair
Reactome:R-HSA-6782135 Dual incision in TC-NER
Reactome:R-HSA-73886 Chromosome Maintenance
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-162587 HIV Life Cycle
Reactome:R-HSA-167172 Transcription of the HIV genome
Reactome:R-HSA-167169 HIV Transcription Elongation
Reactome:R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
Reactome:R-HSA-168270 Fusion and Uncoating of the Influenza Virion
Reactome:R-HSA-168273 Influenza Viral RNA Transcription and Replication
Reactome:R-HSA-192814 vRNA Synthesis
Reactome:R-HSA-168333 NEP/NS2 Interacts with the Cellular Export Machinery
Reactome:R-HSA-168303 Packaging of Eight RNA Segments
Reactome:R-HSA-74160 Gene expression (Transcription)
Reactome:R-HSA-73857 RNA Polymerase II Transcription
Reactome:R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
Reactome:R-HSA-75955 RNA Polymerase II Transcription Elongation
Reactome:R-MMU-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
Reactome:R-MMU-74160 Gene expression (Transcription)
Reactome:R-MMU-73857 RNA Polymerase II Transcription
Reactome:R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
Reactome:R-MMU-162582 Signaling Pathways
Reactome:R-MMU-8939211 ESR-mediated signaling
Reactome:R-BTA-674695 RNA Polymerase II Pre-transcription Events
Reactome:R-BTA-73776 RNA Polymerase II Promoter Escape
Reactome:R-BTA-211000 Gene Silencing by RNA
Reactome:R-BTA-5578749 Transcriptional regulation by small RNAs
Reactome:R-BTA-73894 DNA Repair
Reactome:R-BTA-6781823 Formation of TC-NER Pre-Incision Complex
Reactome:R-BTA-162582 Signaling Pathways
Reactome:R-HSA-199991 Membrane Trafficking
Reactome:R-HSA-8854214 TBC/RABGAPs
Reactome:R-HSA-1266738 Developmental Biology
Reactome:R-HSA-6781823 Formation of TC-NER Pre-Incision Complex
Reactome:R-HSA-9006931 Signaling by Nuclear Receptors
Reactome:R-HSA-9018519 Estrogen-dependent gene expression
Reactome:R-HSA-1640170 Cell Cycle
Reactome:R-HSA-157579 Telomere Maintenance
Reactome:R-HSA-162906 HIV Infection
Reactome:R-HSA-162599 Late Phase of HIV Life Cycle
Reactome:R-HSA-168275 Entry of Influenza Virion into Host Cell via Endocytosis
Reactome:R-HSA-168271 Transport of Ribonucleoproteins into the Host Nucleus
Reactome:R-HSA-192869 cRNA Synthesis
Reactome:R-HSA-168330 Viral RNP Complexes in the Host Cell Nucleus
Reactome:R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
Reactome:R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
Reactome:R-MMU-674695 RNA Polymerase II Pre-transcription Events
Reactome:R-MMU-73776 RNA Polymerase II Promoter Escape
Reactome:R-BTA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
Reactome:R-BTA-75955 RNA Polymerase II Transcription Elongation
Reactome:R-BTA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
Reactome:R-BTA-9006931 Signaling by Nuclear Receptors
Reactome:R-BTA-9018519 Estrogen-dependent gene expression
Reactome:R-HSA-9007101 Rab regulation of trafficking
Reactome:R-HSA-5619507 Activation of HOX genes during differentiation
Reactome:R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
Reactome:R-HSA-167161 HIV Transcription Initiation
Reactome:R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
Reactome:R-HSA-167287 HIV elongation arrest and recovery
Reactome:R-HSA-168288 Fusion of the Influenza Virion to the Host Cell Endosome
Reactome:R-HSA-192905 vRNP Assembly
Reactome:R-HSA-192823 Viral mRNA Translation
Reactome:R-HSA-168268 Virus Assembly and Release
Reactome:R-HSA-168302 Budding
Reactome:R-HSA-113418 Formation of the Early Elongation Complex
Reactome:R-HSA-6807505 RNA polymerase II transcribes snRNA genes
Reactome:R-HSA-211000 Gene Silencing by RNA
Reactome:R-HSA-5578749 Transcriptional regulation by small RNAs
Reactome:R-MMU-73894 DNA Repair
Reactome:R-MMU-6781823 Formation of TC-NER Pre-Incision Complex
Reactome:R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
Reactome:R-MMU-75955 RNA Polymerase II Transcription Elongation
Reactome:R-MMU-9006931 Signaling by Nuclear Receptors
Reactome:R-MMU-9018519 Estrogen-dependent gene expression
Reactome:R-BTA-113418 Formation of the Early Elongation Complex
Reactome:R-BTA-6807505 RNA polymerase II transcribes snRNA genes
Reactome:R-BTA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
Reactome:R-HSA-5653656 Vesicle-mediated transport
Reactome:R-HSA-5617472 Activation of anterior HOX genes in hindbrain development during early embryogenesis
Reactome:R-HSA-73894 DNA Repair
Reactome:R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
Reactome:R-HSA-162582 Signaling Pathways
Reactome:R-HSA-8939211 ESR-mediated signaling
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-HSA-167162 RNA Polymerase II HIV Promoter Escape
Reactome:R-HSA-168255 Influenza Infection
Reactome:R-HSA-168336 Uncoating of the Influenza Virion
Reactome:R-HSA-168325 Viral Messenger RNA Synthesis
Reactome:R-HSA-168274 Export of Viral Ribonucleoproteins from Nucleus
Reactome:R-HSA-168298 Release
Reactome:R-HSA-674695 RNA Polymerase II Pre-transcription Events
Reactome:R-HSA-73776 RNA Polymerase II Promoter Escape
Reactome:R-HSA-203927 MicroRNA (miRNA) biogenesis
Reactome:R-HSA-5601884 PIWI-interacting RNA (piRNA) biogenesis
Reactome:R-MMU-5696398 Nucleotide Excision Repair
Reactome:R-MMU-6782135 Dual incision in TC-NER
Reactome:R-MMU-113418 Formation of the Early Elongation Complex
Reactome:R-MMU-6807505 RNA polymerase II transcribes snRNA genes
WikiPathways:WP5183 SARS-CoV-2 mitochondrial chronic oxidative stress and endothelial dysfunction
WikiPathways:WP5021 SARS-CoV-2 Infection
WikiPathways:WP5113 Antiviral and anti-inflammatory effects of Nrf2 on SARS-CoV-2 pathway
WikiPathways:WP4846 SARS-CoV-2 and COVID-19 pathway
WikiPathways:WP5156 SARS-CoV-2 replication organelle formation
WikiPathways:WP5020 SARS-CoV-1 Infection
WikiPathways:WP5038 Mitochondrial immune response to SARS-CoV-2