EC: 2.7.2.8

acetylglutamate kinase (ATP:N-acetyl-L-glutamate 5-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q9SCL7 [2-Oxoglutarate]

ATP + N_Acetyl_L_glutamate -> ADP + N_Acetyl_L_glutamyl_5_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl, 20 mM MgCl2, 300 mM Hydroxylaminhydrochlorid
PH: 7.5
Temperature: 37
uniprot:Q9SCL7 [2-Oxoglutarate]

N_Acetyl_L_glutamate + ATP -> ADP + N_Acetyl_L_glutamyl_5_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl, 20 mM MgCl2, 300 mM Hydroxylaminhydrochlorid
PH: 7.5
Temperature: 37
uniprot:Q9SCL7 [acetylglutamate kinase(Enzyme) wildtype His6-tag]

N_Acetyl_L_glutamate + ATP -> N_Acetyl_L_glutamyl_5_phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl, 20 mM MgCl2, 300 mM Hydroxylaminhydrochlorid
PH: 7.5
Temperature: 37
uniprot:Q9SCL7 [acetylglutamate kinase(Enzyme) wildtype His6-tag]

ATP + N_Acetyl_L_glutamate -> ADP + N_Acetyl_L_glutamyl_5_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl, 20 mM MgCl2, 300 mM Hydroxylaminhydrochlorid
PH: 7.5
Temperature: 37
uniprot:Q9SCL7 [acetylglutamate kinase(Enzyme) wildtype His6-tag]

N_Acetyl_L_glutamate + ATP -> N_Acetyl_L_glutamyl_5_phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl, 20 mM MgCl2, 300 mM Hydroxylaminhydrochlorid
PH: 7.5
Temperature: 37
uniprot:Q9SCL7 [acetylglutamate kinase(Enzyme) wildtype His6-tag]

ATP + N_Acetyl_L_glutamate -> ADP + N_Acetyl_L_glutamyl_5_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl, 20 mM MgCl2, 300 mM Hydroxylaminhydrochlorid
PH: 7.5
Temperature: 37
uniprot:P0A6C8 [acetylglutamate kinase(Enzyme) wildtype His6-tagged]

N_Acetyl_L_glutamate + ATP -> N_Acetyl_L_glutamyl_5_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate, 20 mM MgCl2, 0.2 mM NADPH, 10 µg/ml N-Acetyl-gamma-glutamyl-phosphate reductase
PH: 7
Temperature: 30
uniprot:P0A6C8 [acetylglutamate kinase(Enzyme) wildtype His6-tagged]

ATP + N_Acetyl_L_glutamate -> N_Acetyl_L_glutamyl_5_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate, 20 mM MgCl2, 0.2 mM NADPH, 10 µg/ml N-Acetyl-gamma-glutamyl-phosphate reductase
PH: 7
Temperature: 30
uniprot:P0A6C8 [acetylglutamate kinase(Enzyme) wildtype]

N_Acetyl_L_glutamate + ATP -> N_Acetyl_L_glutamyl_5_phosphate + ADP		 (( (Vmax * A) ) / (( (A + Km) ) + (( (A ^ 2) ) / Ki))) buffer: 20 mM sodium phosphate, 10 mM beta-mercaptoethanol, 0.3 mM NADH, 1.5 mM phosphoenolpyruvate, 50 mM KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 10 mM MgCl
PH: 7
Temperature: 25
uniprot:P0A6C8 [acetylglutamate kinase(Enzyme) wildtype]

ATP + N_Acetyl_L_glutamate -> ADP + N_Acetyl_L_glutamyl_5_phosphate		 (( (Vmax * A) ) / (( (A + Km) ) + (( (A ^ 2) ) / Ki))) buffer: 20 mM sodium phosphate, 10 mM beta-mercaptoethanol, 0.3 mM NADH, 1.5 mM phosphoenolpyruvate, 50 mM KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 10 mM MgCl
PH: 7
Temperature: 25
uniprot:P0A6C8 [acetylglutamate kinase(Enzyme) mutant R66K]

ATP + N_Acetyl_L_glutamate -> ADP + N_Acetyl_L_glutamyl_5_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM sodium phosphate, 10 mM beta-mercaptoethanol, 0.3 mM NADH, 1.5 mM phosphoenolpyruvate, 50 mM KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 20 mM MgCl2
PH: 7
Temperature: 25
uniprot:P0A6C8 [acetylglutamate kinase(Enzyme) wildtype]

ATP + N_Acetyl_L_glutamate -> N_Acetyl_L_glutamyl_5_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM sodium phosphate, 10 mM beta-mercaptoethanol, 0.3 mM NADH, 1.5 mM phosphoenolpyruvate, 50 mM KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 20 mM MgCl2
PH: 7
Temperature: 25
uniprot:P0A6C8 [acetylglutamate kinase(Enzyme) mutant D162E]

ATP + N_Acetyl_L_glutamate -> N_Acetyl_L_glutamyl_5_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM sodium phosphate, 10 mM beta-mercaptoethanol, 0.3 mM NADH, 1.5 mM phosphoenolpyruvate, 50 mM KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 20 mM MgCl2
PH: 7
Temperature: 25
uniprot:P0A6C8 [acetylglutamate kinase(Enzyme) mutant K8R]

N_Acetyl_L_glutamate + ATP -> ADP + N_Acetyl_L_glutamyl_5_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM sodium phosphate, 10 mM beta-mercaptoethanol, 0.3 mM NADH, 1.5 mM phosphoenolpyruvate, 50 mM KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 20 mM MgCl2
PH: 7
Temperature: 25
uniprot:P0A6C8 [acetylglutamate kinase(Enzyme) mutant R66K]

ATP + N_Acetyl_L_glutamate -> ADP + N_Acetyl_L_glutamyl_5_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM sodium phosphate, 10 mM beta-mercaptoethanol, 0.3 mM NADH, 1.5 mM phosphoenolpyruvate, 50 mM KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 20 mM MgCl2
PH: 7
Temperature: 25
uniprot:P0A6C8 [acetylglutamate kinase(Enzyme) mutant N158Q]

ATP + N_Acetyl_L_glutamate -> N_Acetyl_L_glutamyl_5_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM sodium phosphate, 10 mM beta-mercaptoethanol, 0.3 mM NADH, 1.5 mM phosphoenolpyruvate, 50 mM KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 20 mM MgCl2
PH: 7
Temperature: 25
uniprot:P0A6C8 [acetylglutamate kinase(Enzyme) mutant R66K]

N_Acetyl_L_glutamate + ATP -> ADP + N_Acetyl_L_glutamyl_5_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM sodium phosphate, 10 mM beta-mercaptoethanol, 0.3 mM NADH, 1.5 mM phosphoenolpyruvate, 50 mM KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 10 mM MgCl
PH: 7
Temperature: 25
uniprot:P0A6C8 [acetylglutamate kinase(Enzyme) mutant N158Q]

ATP + N_Acetyl_L_glutamate -> N_Acetyl_L_glutamyl_5_phosphate + ADP		 (( (Vmax * A) ) / (( (A + Km) ) + (( (A ^ 2) ) / Ki))) buffer: 20 mM sodium phosphate, 10 mM beta-mercaptoethanol, 0.3 mM NADH, 1.5 mM phosphoenolpyruvate, 50 mM KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 10 mM MgCl
PH: 7
Temperature: 25
uniprot:P0A6C8 [acetylglutamate kinase(Enzyme) mutant D162E]

N_Acetyl_L_glutamate + ATP -> ADP + N_Acetyl_L_glutamyl_5_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM sodium phosphate, 10 mM beta-mercaptoethanol, 0.3 mM NADH, 1.5 mM phosphoenolpyruvate, 50 mM KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 10 mM MgCl
PH: 7
Temperature: 25
uniprot:P0A6C8 [acetylglutamate kinase(Enzyme) mutant K8R]

ATP + N_Acetyl_L_glutamate -> ADP + N_Acetyl_L_glutamyl_5_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM sodium phosphate, 10 mM beta-mercaptoethanol, 0.3 mM NADH, 1.5 mM phosphoenolpyruvate, 50 mM KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 10 mM MgCl
PH: 7
Temperature: 25

Pathways

pathway id name
BioCyc:ECO_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:ARA_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:VCHO_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:MTBH37RV_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:MTBCDC1551_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:MTBCDC1551_PWY-7400 L-arginine biosynthesis IV (archaebacteria)
BioCyc:ECOO157_GLUTORN-PWY ornithine biosynthesis
BioCyc:ANTHRA_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:AGRO_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:SYNEL_GLUTORN-PWY L-ornithine biosynthesis
BioCyc:ECOL316407_GLUTORN-PWY ornithine biosynthesis
BioCyc:ECOL316407_ARG+POLYAMINE-SYN superpathway of arginine and polyamine biosynthesis
BioCyc:PDIF272563_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:ECO_ARG+POLYAMINE-SYN superpathway of arginine and polyamine biosynthesis
BioCyc:ECO_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:MTBH37RV_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:MTBCDC1551_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:ECOO157_PWY-7400 arginine biosynthesis IV (archaebacteria)
BioCyc:CAULO_GLUTORN-PWY ornithine biosynthesis
BioCyc:ANTHRA_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:ANTHRA_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:AGRO_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:PDIF272563_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:CORYNE_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:CORYNE_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:META_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:META_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:ARA_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:CAULO_ARGSYNBSUB-PWY arginine biosynthesis II (acetyl cycle)
BioCyc:ECOL316407_ARGSYN-PWY arginine biosynthesis I (via L-ornithine)
BioCyc:CAULONA1000_ARGSYN-PWY arginine biosynthesis I
BioCyc:CAULONA1000_ARGSYNBSUB-PWY arginine biosynthesis II (acetyl cycle)
BioCyc:PDIF272563_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:BTHE_ARGSYN-PWY arginine biosynthesis I (via L-ornithine)
BioCyc:BTHE_PWY-7400 arginine biosynthesis IV (archaebacteria)
BioCyc:CORYNE_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:META_ARG+POLYAMINE-SYN superpathway of arginine and polyamine biosynthesis
BioCyc:YEAST_ARGSYNBSUB-PWY arginine biosynthesis
BioCyc:ARA_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:VCHO_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:VCHO_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:MTBH37RV_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:MTBCDC1551_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:ECOO157_ARGSYN-PWY arginine biosynthesis I (via L-ornithine)
BioCyc:CAULO_ARGSYN-PWY arginine biosynthesis I
BioCyc:BSUB_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:SYNEL_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:CAULONA1000_GLUTORN-PWY ornithine biosynthesis
BioCyc:BTHE_GLUTORN-PWY ornithine biosynthesis
BioCyc:GCF_000013425_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
PathBank:SMP0000812 Arginine Metabolism
PathBank:SMP0000813 Ornithine Metabolism
PlantCyc:ARA_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
PlantCyc:ARA_GLUTORN-PWY L-ornithine biosynthesis I
PlantCyc:PLANT_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
PlantCyc:PLANT_GLUTORN-PWY L-ornithine biosynthesis I
PlantCyc:ARA_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
PlantCyc:PLANT_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
Reactome:R-SCE-71291 Amino acid and derivative metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SPO-71291 Amino acid and derivative metabolism
Reactome:R-SPO-70635 Urea cycle
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-70635 Urea cycle
Reactome:R-SCE-70635 Urea cycle
PathBank:SMP0002420 Proline Metabolism
PathBank:SMP0121275 Ornithine Metabolism
PathBank:SMP0002416 Arginine Metabolism
PathBank:SMP0121274 Arginine Metabolism