EC: 2.7.13.3

histidine kinase (ATP:protein-L-histidine N-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:A7HD43 [Mn2+]

Protein_histidine + ATP -> Protein_N_phosphohistidine + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 50 mM KCl
PH: 8
Temperature: 20
uniprot:A7HD43 [Ca2+]

Protein_histidine + ATP -> Protein_N_phosphohistidine + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 50 mM KCl
PH: 8
Temperature: 20
uniprot:A7HD43 [histidine kinase(Enzyme) wildtype with heme Fe(III)-OH-]

Protein_histidine + ATP -> Protein_N_phosphohistidine + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 50 mM KCl
PH: 8
Temperature: 20
uniprot:A7HD43 [histidine kinase(Enzyme) wildtype with heme Fe(III)-OH- and wildtype response regulator]

Protein_histidine + ATP -> Protein_N_phosphohistidine + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 50 mM KCl
PH: 8
Temperature: 20
uniprot:A7HD43 [Mg2+]

Protein_histidine + ATP -> Protein_N_phosphohistidine + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 50 mM KCl
PH: 8
Temperature: 20
uniprot:A7HD43 [Mg2+]

Protein_histidine + ATP -> Protein_N_phosphohistidine + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 50 mM KCl
PH: 8
Temperature: 20
uniprot:A7HD43 [Mg2+]

Protein_histidine + ATP -> Protein_N_phosphohistidine + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 50 mM KCl
PH: 8
Temperature: 20
uniprot:A7HD43 [Mg2+]

Protein_histidine + ATP -> Protein_N_phosphohistidine + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 50 mM KCl
PH: 8
Temperature: 20
uniprot:A7HD43 [Mg2+]

Protein_histidine + ATP -> Protein_N_phosphohistidine + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 50 mM KCl
PH: 8
Temperature: 20
uniprot:A7HD43 [Mg2+]

Protein_histidine + ATP -> Protein_N_phosphohistidine + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 50 mM KCl
PH: 8
Temperature: 20
uniprot:A7HD43 [Mg2+]

Protein_histidine + ATP -> Protein_N_phosphohistidine + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 50 mM KCl
PH: 8
Temperature: 20
uniprot:A7HD43 [Mg2+]

Protein_histidine + ATP -> Protein_N_phosphohistidine + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 50 mM KCl
PH: 8
Temperature: 20
uniprot:O69729 [Mg2+]

Protein_histidine + ATP -> Protein_N_phosphohistidine + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2 mM PEP, 30 U/ml LDH, 20 U/ml PK, 200 µM NADH
PH: 7.5
Temperature: 36
uniprot:O07778 [histidine kinase(Enzyme) wildtype]

ATP + Protein_histidine -> ADP + Protein_N_phosphohistidine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 5 µM HK2, 2 mM PEP, 4 mM MgCl2, 30 U/ml L-lactate dehydrogenase, 20 U/ml pyruvate kinase, 150 µM NADH
PH: 7.5
Temperature: 36
uniprot:P23837 [Acetyl-CoA]

PhoQ_cytoplasmic_domain + ATP -> phosphorylated_PhoQ_cytoplasmic_domain + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 10 mM Tris-HCl, 25 mM KCl, 0.4 mM MgCl2
PH: 8
Temperature: 22
uniprot:P23837 [Acetyl-CoA]

PhoQ_cytoplasmic_domain + ATP -> phosphorylated_PhoQ_cytoplasmic_domain + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 10 mM Tris-HCl, 25 mM KCl, 0.4 mM MgCl2
PH: 8
Temperature: 22
uniprot:P23837 [Acetyl-CoA]

PhoQ_cytoplasmic_domain + ATP -> phosphorylated_PhoQ_cytoplasmic_domain + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 10 mM Tris-HCl, 25 mM KCl, 0.4 mM MgCl2
PH: 8
Temperature: 22
uniprot:P23837 [histidine kinase(Enzyme) wildtype cytoplasmic domain]

PhoQ_cytoplasmic_domain + ATP -> phosphorylated_PhoQ_cytoplasmic_domain + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 10 mM Tris-HCl, 25 mM KCl, 0.4 mM MgCl2
PH: 8
Temperature: 22
uniprot:P23837 [histidine kinase(Enzyme) mutant R434A PhoQ cytoplasmic domain (aa 219-486)]

Protein_histidine + ATP -> ADP + Protein_N_phosphohistidine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris-Cl, 50 mM KCl, 1 mM MgCl2
PH: 8
Temperature: 22
uniprot:P23837 [histidine kinase(Enzyme) wildtype PhoQ cytoplasmic domain (aa 219-486)]

Protein_histidine + ATP -> ADP + Protein_N_phosphohistidine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris-Cl, 50 mM KCl, 1 mM MgCl2
PH: 8
Temperature: 22
uniprot:P23837 [histidine kinase(Enzyme) mutant L392A PhoQ cytoplasmic domain (aa 219-486)]

Protein_histidine + ATP -> Protein_N_phosphohistidine + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris-Cl, 50 mM KCl, 1 mM MgCl2
PH: 8
Temperature: 22
uniprot:P23837 [histidine kinase(Enzyme) mutant R439A PhoQ cytoplasmic domain (aa 219-486)]

Protein_histidine + ATP -> ADP + Protein_N_phosphohistidine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris-Cl, 50 mM KCl, 1 mM MgCl2
PH: 8
Temperature: 22

Pathways

pathway id name
BioCyc:HUMAN_PWY-7224 purine deoxyribonucleosides salvage
BioCyc:META_PWY-7227 adenosine deoxyribonucleotides de novo biosynthesis
BioCyc:META_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:META_PWY-7205 CMP phosphorylation
BioCyc:META_PWY66-409 superpathway of purine nucleotide salvage
BioCyc:META_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ECO_PWY0-1512 CusSR Two-Component Signal Transduction System
BioCyc:ECO_PWY0-1483 PhoRB Two-Component Signal Transduction System, phosphate-dependent
BioCyc:ECO_PWY0-1505 ArcAB Two-Component Signal Transduction System, quinone dependent
BioCyc:ECO_PWY0-1474 AtoSC Two-Component Signal Transduction System
BioCyc:ECO_PWY0-1588 HprSR Two-Component Signal Transduction System
BioCyc:ECO_PWY0-1481 BaeSR Two-Component Signal Transduction System
BioCyc:ECO_PWY0-1468 DcuSR Two-Component Signal Transduction System, dicarboxylate-dependent
BioCyc:ECO_PWY0-1550 YpdAB Two-Component Signal Transduction System
BioCyc:ECO_PWY0-1493 RcsCDB Two-Component Signal Transduction System
BioCyc:MOUSE_PWY-5687 pyrimidine ribonucleotides interconversion
BioCyc:MOUSE_PWY0-162 pyrimidine ribonucleotides de novo biosynthesis
BioCyc:BSUB_PWY8J2-17 YdfHI two-component signal transduction system
BioCyc:BSUB_PWY8J2-7 WalKR two-component signal transduction system
BioCyc:BSUB_PWY8J2-12 LytST two-component signal transduction system
BioCyc:BSUB_PWY8J2-16 LiaSR two-component signal transduction system
BioCyc:BSUB_PWY8J2-6 ResED two-component signal transduction system
BioCyc:HUMAN_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7176 UTP and CTP de novo biosynthesis
BioCyc:HUMAN_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7228 guanosine nucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY66-409 superpathway of purine nucleotide salvage
BioCyc:HUMAN_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:HUMAN_PWY-7210 pyrimidine deoxyribonucleotides biosynthesis from CTP
BioCyc:HUMAN_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:META_PWY-7210 pyrimidine deoxyribonucleotides biosynthesis from CTP
BioCyc:META_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:META_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:ECO_PWY0-1482 BasSR Two-Component Signal Transduction System
BioCyc:ECO_PWY0-1499 DpiBA Two-Component Signal Transduction System
BioCyc:ECO_PWY0-1490 EvgSA Two-Component Signal Transduction System
BioCyc:ECO_PWY0-1458 PhoQP Two-Component Signal Transduction System, magnesium-dependent
BioCyc:ECO_PWY0-1492 UhpBA Two Component Signal Transduction System
BioCyc:MOUSE_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:BSUB_PWY8J2-14 DegSU two-component signal transduction system
BioCyc:BSUB_PWY8J2-11 YufLM two-component signal transduction system, malate-dependent
BioCyc:BSUB_PWY8J2-8 BceSR two-component signal transduction system
BioCyc:BSUB_PWY8J2-10 CitST two-component signal transduction system
BioCyc:BSUB_PWY8J2-9 YxdKJ two-component signal transduction system
BioCyc:META_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:META_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:META_PWY-7224 purine deoxyribonucleosides salvage
BioCyc:META_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:ECO_PWY0-1487 CreCB Two-Component Signal Transduction System
BioCyc:ECO_PWY0-1470 QseBC Two-Component Signal Transduction System, quorum sensing related
BioCyc:ECO_PWY0-1497 RstBA Two-Component Signal Transduction System
BioCyc:ECO_PWY0-1514 NarQ Two-Component Signal Transduction System, nitrate dependent
BioCyc:ECO_PWY0-1501 BarA UvrY Two-Component Signal Transduction System
BioCyc:ECO_PWY0-1498 ZraSR Two-Component Signal Transduction System
BioCyc:ECO_PWY0-1515 NarX Two-Component Signal Transduction System, nitrate dependent
BioCyc:BSUB_PWY8J2-4 ComPA two-component signal transduction system
BioCyc:BSUB_PWY8J2-18 DctSR two-component signal transduction system
BioCyc:BSUB_PWY8J2-5 CssSR two-component signal transduction system
BioCyc:BSUB_PWY8J2-13 NatKR two-component signal transduction system
BioCyc:SYNEL_PWY490-14 SphS sensory histidine kinase
BioCyc:SYNEL_PWY490-13 SasA sensory histidine kinase
BioCyc:HUMAN_PWY-7226 guanosine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7227 adenosine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-841 purine nucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7205 CMP phosphorylation
BioCyc:META_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:META_PWY-7176 UTP and CTP de novo biosynthesis
BioCyc:META_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:META_PWY-7226 guanosine deoxyribonucleotides de novo biosynthesis I
BioCyc:ECO_PWY0-1495 KdpDE Two-Component Signal Transduction System, potassium-dependent
BioCyc:ECO_PWY0-1485 CpxAR Two-Component Signal Transduction System
BioCyc:BSUB_PWY8J2-3 PhoPR two-component signal transduction pathway
BioCyc:BSUB_PWY8J2-15 DesKR two-component signal transduction system
PathBank:SMP0002123 BaeSR Two-Component Signal Transduction System
PathBank:SMP0002246 Operon: Methionine Biosynthesis
PathBank:SMP0002254 Operon: Maltodextrins and Maltose Transport Inactivation
PathBank:SMP0002260 Operon: Phosphonate Utilization
PathBank:SMP0002269 Operon: lysine decarboxylase inactivation III
Plant Reactome:R-OSA-5225756 Ethylene mediated signaling
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-2744341 Hormone signaling, transport, and metabolism
Plant Reactome:R-OSA-5225808 Ethylene biosynthesis and signaling
Reactome:R-BTA-9748784 Drug ADME
Reactome:R-CFA-9748787 Azathioprine ADME
Reactome:R-RNO-9748784 Drug ADME
Reactome:R-SSC-9748784 Drug ADME
Reactome:R-CFA-9748784 Drug ADME
Reactome:R-HSA-9748787 Azathioprine ADME
Reactome:R-MMU-9755088 Ribavirin ADME
Reactome:R-BTA-9748787 Azathioprine ADME
Reactome:R-MMU-9748784 Drug ADME
Reactome:R-RNO-9748787 Azathioprine ADME
Reactome:R-SSC-9748787 Azathioprine ADME
Reactome:R-BTA-9755088 Ribavirin ADME
Reactome:R-CFA-9755088 Ribavirin ADME
Reactome:R-RNO-9755088 Ribavirin ADME
Reactome:R-SSC-9755088 Ribavirin ADME
Reactome:R-HSA-9748784 Drug ADME
Reactome:R-MMU-9748787 Azathioprine ADME
Reactome:R-HSA-9755088 Ribavirin ADME
Reactome:R-BTA-1430728 Metabolism
Reactome:R-CFA-1430728 Metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SSC-1430728 Metabolism
Reactome:R-SSC-15869 Nucleotide metabolism
Reactome:R-SSC-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-BTA-15869 Nucleotide metabolism
Reactome:R-BTA-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-CFA-15869 Nucleotide metabolism
Reactome:R-CFA-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-RNO-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-HSA-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-MMU-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-RNO-15869 Nucleotide metabolism
Reactome:R-RNO-499943 Interconversion of nucleotide di- and triphosphates
PathBank:SMP0120862 Congenital Disorder of Glycosylation CDG-IId
PathBank:SMP0000444 Lactose Synthesis
PathBank:SMP0000580 GLUT-1 Deficiency Syndrome
PathBank:SMP0000629 Adefovir Dipivoxil Metabolism Pathway
PathBank:SMP0000649 Lamivudine Metabolism Pathway
PathBank:SMP0087229 Lactose Synthesis
PathBank:SMP0000446 Gemcitabine Action Pathway
PathBank:SMP0000579 Congenital Disorder of Glycosylation CDG-IId
PathBank:SMP0000630 Tenofovir Metabolism Pathway
PathBank:SMP0120863 GLUT-1 Deficiency Syndrome
PathBank:SMP0000603 Gemcitabine Metabolism Pathway
PathBank:SMP0002410 Stress-Activated Signalling Pathways: Low Osmolarity
PathBank:SMP0087323 Lactose Synthesis