EC: 2.6.1.52

phosphoserine transaminase (O-phospho-L-serine:2-oxoglutarate aminotransferase)

enzyme lambda metabolic reaction experiment
uniprot:P23721 [phosphoserine transaminase(Enzyme) wildtype]

3_Phosphohydroxypyruvate + L_Glutamate -> O_Phospho_L_serine + 2_Oxoglutarate		 (( (( (( (cell * serC) ) * kcatC) ) * (php / KCphp)) ) / (( (1 + (php / KCphp)) ) + (pser / KCpser))) buffer: *
PH: 7
Temperature: 36
uniprot:Q60I38 [phosphoserine transaminase(Enzyme) wildtype His-tagged]

O_Phospho_L_serine + 2_Oxoglutarate -> 3_Phosphohydroxypyruvate + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM TAPS, 0.2 mM NADH, 1.0 mM DTT, 1 mM EDTA, 32 mM ammonium acetate, 20 µM pyridoxal 5-phosphate, 14.4 µg/ml D-phosphoglycerate dehydrogenase
PH: 8.5
Temperature: 25
uniprot:Q60I38 [phosphoserine transaminase(Enzyme) wildtype His-tagged]

3_Phosphohydroxypyruvate + L_Glutamate -> 2_Oxoglutarate + O_Phospho_L_serine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM TAPS, 0.1 mM NADH, 1.0 mM DTT, 1 mM EDTA, 32 mM ammonium acetate, 20 µM pyridoxal 5-phosphate, 6.48 U/ml glutamate dehydrogenase
PH: 8.5
Temperature: 25
uniprot:Q60I38 [phosphoserine transaminase(Enzyme) wildtype His-tagged]

3_Phosphohydroxypyruvate + L_Glutamate -> 2_Oxoglutarate + O_Phospho_L_serine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM TAPS, 0.1 mM NADH, 1.0 mM DTT, 1 mM EDTA, 32 mM ammonium acetate, 20 µM pyridoxal 5-phosphate, 6.48 U/ml glutamate dehydrogenase
PH: 8.5
Temperature: 25
uniprot:Q60I38 [phosphoserine transaminase(Enzyme) wildtype His-tagged]

O_Phospho_L_serine + 2_Oxoglutarate -> 3_Phosphohydroxypyruvate + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM TAPS, 0.2 mM NADH, 1.0 mM DTT, 1 mM EDTA, 32 mM ammonium acetate, 20 µM pyridoxal 5-phosphate, 14.4 µg/ml D-phosphoglycerate dehydrogenase
PH: 8.5
Temperature: 25
uniprot:Q96255 [phosphoserine transaminase(Enzyme) wildtype His-tagged]

2_Oxoglutarate + O_Phospho_L_serine -> 3_Phosphohydroxypyruvate + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM TAPS, 0.2 mM NADH, 1.0 mM DTT, 1 mM EDTA, 32 mM ammonium acetate, 20 µM pyridoxal 5-phosphate, 36 µg/ml D-phosphoglycerate dehydrogenase
PH: 8.5
Temperature: 25
uniprot:Q96255 [phosphoserine transaminase(Enzyme) wildtype His-tagged]

3_Phosphohydroxypyruvate + L_Glutamate -> 2_Oxoglutarate + O_Phospho_L_serine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM TAPS, 0.1 mM NADH, 1.0 mM DTT, 1 mM EDTA, 32 mM ammonium acetate, 20 µM pyridoxal 5-phosphate, 6.48 U/ml glutamate dehydrogenase
PH: 8.5
Temperature: 25
uniprot:Q96255 [phosphoserine transaminase(Enzyme) wildtype His-tagged]

3_Phosphohydroxypyruvate + L_Glutamate -> O_Phospho_L_serine + 2_Oxoglutarate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM TAPS, 0.1 mM NADH, 1.0 mM DTT, 1 mM EDTA, 32 mM ammonium acetate, 20 µM pyridoxal 5-phosphate, 6.48 U/ml glutamate dehydrogenase
PH: 8.5
Temperature: 25
uniprot:Q96255 [phosphoserine transaminase(Enzyme) wildtype His-tagged]

2_Oxoglutarate + O_Phospho_L_serine -> L_Glutamate + 3_Phosphohydroxypyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM TAPS, 0.2 mM NADH, 1.0 mM DTT, 1 mM EDTA, 32 mM ammonium acetate, 20 µM pyridoxal 5-phosphate, 36 µg/ml D-phosphoglycerate dehydrogenase
PH: 8.5
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_SERSYN-PWY serine biosynthesis (phosphorylated route)
BioCyc:META_PWY-6643 coenzyme M biosynthesis II
BioCyc:META_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:META_SER-GLYSYN-PWY superpathway of L-serine and glycine biosynthesis I
BioCyc:META_PWY0-781 aspartate superpathway
BioCyc:ECO_SERSYN-PWY L-serine biosynthesis I
BioCyc:MTBH37RV_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:ECOO157_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:ECOL199310_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:ECOL316407_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:BTHE_SER-GLYSYN-PWY superpathway of serine and glycine biosynthesis I
BioCyc:BTHE_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:META_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:META_SERSYN-PWY L-serine biosynthesis I
BioCyc:META_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:META_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:ECO_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
BioCyc:ECO_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:YEAST_SER-GLYSYN-PWY superpathway of serine and glycine biosynthesis I
BioCyc:MTBH37RV_SERSYN-PWY L-serine biosynthesis I
BioCyc:MTBCDC1551_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:MTBCDC1551_SER-GLYSYN-PWY superpathway of L-serine and glycine biosynthesis I
BioCyc:MTBCDC1551_SERSYN-PWY L-serine biosynthesis
BioCyc:ECOO157_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:MOUSE_SER-GLYSYN-PWY superpathway of serine and glycine biosynthesis I
BioCyc:BSUB_SER-GLYSYN-PWY superpathway of L-serine and glycine biosynthesis I
BioCyc:BTHE_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:ECO_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:ECO_SER-GLYSYN-PWY superpathway of L-serine and glycine biosynthesis I
BioCyc:MTBH37RV_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:ECOO157_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:ECOO157_SER-GLYSYN-PWY superpathway of serine and glycine biosynthesis I
BioCyc:ECOL199310_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:BSUB_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:BSUB_SERSYN-PWY L-serine biosynthesis I
BioCyc:BTHE_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:HUMAN_SER-GLYSYN-PWY serine and glycine biosynthesis
BioCyc:META_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
BioCyc:ECO_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:ECO_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:ECO_PWY0-781 aspartate superpathway
BioCyc:YEAST_SERSYN-PWY serine biosynthesis from 3-phosphoglycerate
BioCyc:ARA_SERSYN-PWY L-serine biosynthesis
BioCyc:MTBH37RV_SER-GLYSYN-PWY superpathway of L-serine and glycine biosynthesis I
BioCyc:ECOO157_SERSYN-PWY serine biosynthesis
BioCyc:ECOL199310_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:ECOL199310_SER-GLYSYN-PWY superpathway of serine and glycine biosynthesis I
BioCyc:ECOL199310_SERSYN-PWY serine biosynthesis
BioCyc:MOUSE_SERSYN-PWY serine biosynthesis
BioCyc:ECOL316407_SERSYN-PWY serine biosynthesis
BioCyc:ECOL316407_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:ECOL316407_SER-GLYSYN-PWY superpathway of serine and glycine biosynthesis I
BioCyc:ECOL316407_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:BTHE_SERSYN-PWY serine biosynthesis
PathBank:SMP0000821 Cysteine Biosynthesis
PathBank:SMP0000829 Serine Biosynthesis and Metabolism
PathBank:SMP0000993 Secondary Metabolites: Cysteine Biosynthesis from Serine
PlantCyc:ARA_SERSYN-PWY L-serine biosynthesis I
PlantCyc:PLANT_SERSYN-PWY L-serine biosynthesis I
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SCE-71291 Amino acid and derivative metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-CEL-977347 Serine metabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-71291 Amino acid and derivative metabolism
Reactome:R-HSA-977347 Serine metabolism
Reactome:R-MMU-977347 Serine metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SPO-71291 Amino acid and derivative metabolism
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-DDI-977347 Serine metabolism
Reactome:R-DME-977347 Serine metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-SPO-977347 Serine metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SCE-977347 Serine metabolism
WikiPathways:WP4688 Serine metabolism
WikiPathways:WP5355 Metabolic Epileptic Disorders
WikiPathways:WP5312 Metabolic pathways of fibroblasts
PathBank:SMP0000242 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0000223 Non-Ketotic Hyperglycinemia
PathBank:SMP0000484 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0087466 Glycine and Serine Metabolism
PathBank:SMP0120653 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0120475 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0000004 Glycine and Serine Metabolism
PathBank:SMP0000721 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0002323 Serine Metabolism
PathBank:SMP0002428 Glycine Metabolism
PathBank:SMP0120521 Sarcosinemia
PathBank:SMP0000179 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0000485 Hyperglycinemia, Non-Ketotic
PathBank:SMP0002320 Glycine Metabolism
PathBank:SMP0002443 Serine Metabolism
PathBank:SMP0063472 Vitamin B6 Metabolism
PathBank:SMP0120474 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0120534 Non-Ketotic Hyperglycinemia
PathBank:SMP0120550 Hyperglycinemia, Non-Ketotic
PathBank:SMP0121282 Cysteine Biosynthesis
PathBank:SMP0121330 Secondary Metabolites: Cysteine Biosynthesis from Serine
PathBank:SMP0000244 Sarcosinemia
PathBank:SMP0063626 Glycine and Serine Metabolism