EC: 2.6.1.5

tyrosine transaminase (L-tyrosine:2-oxoglutarate aminotransferase)

enzyme lambda metabolic reaction experiment
- [Pyridoxal phosphate]

L_Phenylalanine + 2_Oxoglutarate -> L_Glutamate + Phenylpyruvate		 (( (kcat * E) ) / (( (1 + (Km1 / S1)) ) + (Km2 / S2))) buffer: 200 mM Taps, 100 mM KCl, 150 µM NADH, 18 nM - 14 µM 2-hydroxyisocaproate dehydrogenase
PH: 8.25
Temperature: 25
uniprot:P04694 [tyrosine transaminase(Enzyme) wildtype]

L_Tyrosine + 2_Oxoglutarate -> 3_(4_Hydroxyphenyl)pyruvate + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P04694 [tyrosine transaminase(Enzyme) wildtype]

L_Tyrosine + 2_Oxoglutarate -> L_Glutamate + 3_(4_Hydroxyphenyl)pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P04694 [tyrosine transaminase(Enzyme) mutant R315K]

2_Oxoglutarate + L_Tyrosine -> L_Glutamate + 3_(4_Hydroxyphenyl)pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P04694 [tyrosine transaminase(Enzyme) mutant R315K]

2_Oxoglutarate + L_Tyrosine -> L_Glutamate + 3_(4_Hydroxyphenyl)pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P04694 [tyrosine transaminase(Enzyme) mutant N54S]

L_Tyrosine + 2_Oxoglutarate -> L_Glutamate + 3_(4_Hydroxyphenyl)pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P04694 [tyrosine transaminase(Enzyme) mutant N54S]

3_(4_Hydroxyphenyl)pyruvate + L_Glutamate -> 2_Oxoglutarate + L_Tyrosine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P04694 [tyrosine transaminase(Enzyme) wildtype]

L_Glutamate + 3_(4_Hydroxyphenyl)pyruvate -> L_Tyrosine + 2_Oxoglutarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P04694 [tyrosine transaminase(Enzyme) mutant R315K]

3_(4_Hydroxyphenyl)pyruvate + L_Glutamate -> 2_Oxoglutarate + L_Tyrosine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P04694 [tyrosine transaminase(Enzyme) wildtype]

3_(4_Hydroxyphenyl)pyruvate + L_Glutamate -> L_Tyrosine + 2_Oxoglutarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P04694 [tyrosine transaminase(Enzyme) mutant R315K]

L_Glutamate + 3_(4_Hydroxyphenyl)pyruvate -> L_Tyrosine + 2_Oxoglutarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P04694 [tyrosine transaminase(Enzyme) mutant N54S]

L_Glutamate + 3_(4_Hydroxyphenyl)pyruvate -> L_Tyrosine + 2_Oxoglutarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P04694 [tyrosine transaminase(Enzyme) mutant N54S]

L_Tyrosine + 2_Oxoglutarate -> 3_(4_Hydroxyphenyl)pyruvate + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:O85746 [Pyridoxal phosphate]

2_Oxoglutarate + L_Tyrosine -> 3_(4_Hydroxyphenyl)pyruvate + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 10 mM phosphate
PH: 7.4
Temperature: 37
uniprot:O85746 [Pyridoxal phosphate]

4_Methylthio_2_oxobutanoate + L_Glutamate -> L_Methionine + 2_Oxoglutarate		 (( (Vmax * S) ) / (Km + S)) buffer: 10 mM phosphate
PH: 7.4
Temperature: 37
uniprot:O85746 [Pyridoxal phosphate]

L_Tyrosine + 4_Methylthio_2_oxobutanoate -> L_Methionine + 3_(4_Hydroxyphenyl)pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 10 mM phosphate
PH: 7.4
Temperature: 37
uniprot:O85746 [Pyridoxal phosphate]

4_Methylthio_2_oxobutanoate + L_Tyrosine -> 3_(4_Hydroxyphenyl)pyruvate + L_Methionine		 (( (Vmax * S) ) / (Km + S)) buffer: 10 mM phosphate
PH: 7.4
Temperature: 37
uniprot:O85746 [Pyridoxal phosphate]

L_Tyrosine + Pyruvate -> 3_(4_Hydroxyphenyl)pyruvate + L_Alanine		 (( (Vmax * S) ) / (Km + S)) buffer: 10 mM phosphate
PH: 7.4
Temperature: 37
uniprot:O85746 [Pyridoxal phosphate]

L_Tyrosine + Oxaloacetate -> 3_(4_Hydroxyphenyl)pyruvate + L_Aspartate		 (( (Vmax * S) ) / (Km + S)) buffer: 10 mM phosphate
PH: 7.4
Temperature: 37

Pathways

pathway id name
BioCyc:META_TYRFUMCAT-PWY L-tyrosine degradation I
BioCyc:META_PWY-5754 4-hydroxybenzoate biosynthesis I (eukaryotes)
BioCyc:ARA_TYRFUMCAT-PWY L-tyrosine degradation I
BioCyc:HUMAN_PWY-5754 4-hydroxybenzoate biosynthesis
BioCyc:META_PWY-7518 atromentin biosynthesis
BioCyc:HUMAN_TYRFUMCAT-PWY tyrosine degradation
BioCyc:META_PWY-5886 4-hydroxyphenylpyruvate biosynthesis
BioCyc:ARA_PWY-5886 4-hydroxyphenylpyruvate biosynthesis
BioCyc:MOUSE_PWY-5079 phenylalanine degradation III
BioCyc:MOUSE_TYRFUMCAT-PWY tyrosine degradation I
PlantCyc:ARA_TYRFUMCAT-PWY L-tyrosine degradation I
PlantCyc:ARA_PWY-5864 superpathway of plastoquinol biosynthesis
PlantCyc:ARA_PWY-581 indole-3-acetate biosynthesis II
PlantCyc:ARA_PWYDQC-4 indole-3-acetate biosynthesis I
PlantCyc:ARA_PWY-5886 4-hydroxyphenylpyruvate biosynthesis
Reactome:R-RNO-8963684 Tyrosine catabolism
Reactome:R-HSA-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-CEL-8963684 Tyrosine catabolism
Reactome:R-BTA-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-DDI-8963684 Tyrosine catabolism
Reactome:R-MMU-8963684 Tyrosine catabolism
Reactome:R-RNO-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-HSA-8963684 Tyrosine catabolism
Reactome:R-CEL-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-BTA-8963684 Tyrosine catabolism
Reactome:R-DDI-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-MMU-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP4506 Tyrosine metabolism and related disorders
PathBank:SMP0000369 Tyrosinemia Type 2 (or Richner-Hanhart Syndrome)
PathBank:SMP0087450 Tyrosine Metabolism
PathBank:SMP0000206 Phenylketonuria
PathBank:SMP0063474 Tropane, Piperidine, and Pyridine Alkaloid Biosynthesis
PathBank:SMP0000370 Tyrosinemia Type 3 (TYRO3)
PathBank:SMP0087316 Glucose-Alanine Cycle
PathBank:SMP0000008 Phenylalanine and Tyrosine Metabolism
PathBank:SMP0087218 Phenylalanine and Tyrosine Metabolism
PathBank:SMP0087235 Tyrosine Metabolism
PathBank:SMP0063471 Isoquinoline Alkaloid Biosynthesis