EC: 2.5.1.48

cystathionine gamma-synthase (O4-succinyl-L-homoserine:L-cysteine S-(3-amino-3-carboxypropyl)transferase)

enzyme lambda metabolic reaction experiment
uniprot:P55217 [Pyridoxal phosphate]

L_Cysteine + O_Phospho_L_homoserine -> Phosphate + L_Cystathionine		 (( (Vmax * A) ) / (KmA + (A * (1 + (KmB / B))))) buffer: 20 mM Mops/NaOH, 1 mM Dithiothreitol
PH: 7.5
Temperature: 30
uniprot:Q1M0P5 [Pyridoxal phosphate]

O_Succinyl_L_homoserine + H2O -> Succinate + NH3 + 2_Oxobutyrate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 0.7 mM NADH, 21 µM D-2-Hydroxyisocaproate dehydrogenase
PH: 9
Temperature: 25
uniprot:P00935 [Pyridoxal phosphate]

O_Succinyl_L_homoserine + H2O -> Succinate + NH3 + 2_Oxobutyrate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 0.7 mM NADH, 21 µM D-2-Hydroxyisocaproate dehydrogenase
PH: 9
Temperature: 25
uniprot:P00935 [cystathionine gamma-synthase(Enzyme) wildtype]

O_Succinyl_L_homoserine + L_Cysteine -> L_Cystathionine + Succinate		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (( (Kib * Kma) ) * (1 + (A / Kia))) ) + (Kma * B)) ) + (( (Kmb * A) ) * (1 + (A / Kia)))) ) + (A * B))) buffer: 50 mM Tris, 20 µM pyridoxal 5'-phosphate, 0.84 µM E.coli cystathionine Я-lyase, 1.8 µM L-Lactate dehydrogenase, 200 µM NADH
PH: 7.8
Temperature: 25
uniprot:P00935 [cystathionine gamma-synthase(Enzyme) wildtype]

O_Succinyl_L_homoserine -> NH3 + Succinate + Pyruvate		 (( (( (E * (kcat / Km)) ) * A) ) / (1 + (A / Km))) buffer: 50 mM Tris, 20 µM pyridoxal 5'-phosphate, 21 µM D-2-Hydroxyisocaproate dehydrogenase, 200 µM NADH
PH: 7.8
Temperature: 25
uniprot:P00935 [cystathionine gamma-synthase(Enzyme) wildtype]

2 O_Succinyl_L_homoserine + L_Cysteine -> Pyruvate + 2 Succinate + L_Cystathionine + NH3		 (E * (( (( (( (( (kcat_E * Kma_R) ) / Kmb_R) ) * B) ) + (( (( (kcat_R / Kmb_R) ) * B) ) * A)) ) / (( (( (( (( (Kmb_E * Kma_R) ) / Kmb_R) ) + (( (Kma_R / Kmb_R) ) * B)) ) + (( (1 + (A / Kia_R)) ) * A)) ) + (( (( (1 / Kmb_R) ) * B) ) * A)))) buffer: 50 mM Tris, 20 µM pyridoxal 5'-phosphate, 0.84 µM E.coli cystathionine Я-lyase, 1.8 µM L-Lactate dehydrogenase, 200 µM NADH, 21 µM D-2-Hydroxyisocaproate dehydrogenase
PH: 7.8
Temperature: 25
uniprot:P00935 [cystathionine gamma-synthase(Enzyme) wildtype]

L_Cysteine + O_Succinyl_L_homoserine -> L_Cystathionine + Succinate		 (( (( (E * (( (kcat / (1 + (10 ^ (pKa1 - pH)))) ) / Km)) ) * A) ) / (1 + (A / Km))) buffer: 50 mM MOPS (pKa 7.2), 50 mM Bicine (pKa 8.3), 50 mM Proline(pKa 10.7), 20 µM pyridoxal 5'-phosphate, 21 µM D-2-Hydroxyisocaproate dehydrogenase, 200 µM NADH, 0.7-14 µM E.coli cystathionine Я-lyase, 4.1 µM L-Lactate dehydrogenase, 200 µM NADH
PH: 6.6
Temperature: 25
uniprot:P00935 [cystathionine gamma-synthase(Enzyme) wildtype]

O_Succinyl_L_homoserine -> Succinate + NH3 + Pyruvate		 (( (( (E * (( (kcat / (1 + (10 ^ (pKa1 - pH)))) ) / Km)) ) * A) ) / (1 + (A / Km))) buffer: 50 mM MOPS (pKa 7.2), 50 mM Bicine (pKa 8.3), 50 mM Proline(pKa 10.7), 20 µM pyridoxal 5'-phosphate, 21 µM D-2-Hydroxyisocaproate dehydrogenase, 200 µM NADH
PH: 6.1
Temperature: 25
uniprot:P00935 [cystathionine gamma-synthase(Enzyme) wildtype]

O_Succinyl_L_homoserine -> Succinate + Pyruvate + NH3		 (( (( (E * (kcat / Km)) ) * A) ) / (1 + (A / Km))) buffer: *
PH: 7
Temperature: 36
uniprot:Q7M1J3 [cystathionine gamma-synthase(Enzyme) wildtype]

L_Cysteine + O_Succinyl_L_homoserine -> Succinate + L_Cystathionine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Mops-NaOH, 1 mM DTT
PH: 7.5
Temperature: 30
uniprot:Q7M1J3 [cystathionine gamma-synthase(Enzyme) wildtype]

L_Cysteine + O_Phospho_L_homoserine -> L_Cystathionine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Mops-NaOH, 1 mM DTT
PH: 7.5
Temperature: 30
uniprot:Q7M1J3 [cystathionine gamma-synthase(Enzyme) wildtype]

Sulfide + O_Phospho_L_homoserine -> L_Homocysteine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Mops-NaOH, 1 mM DTT
PH: 7.5
Temperature: 30
uniprot:Q7M1J3 [cystathionine gamma-synthase(Enzyme) wildtype]

O_Phospho_L_homoserine + L_Cysteine -> Phosphate + L_Cystathionine		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM Mops-NaOH, 1 mM DTT
PH: 7.5
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-801 homocysteine and cysteine interconversion
BioCyc:META_PWY0-781 aspartate superpathway
BioCyc:ECO_HOMOSER-METSYN-PWY L-methionine biosynthesis I
BioCyc:ECOL316407_P4-PWY superpathway of lysine, threonine and methionine biosynthesis I
BioCyc:ECOL316407_METSYN-PWY homoserine and methionine biosynthesis
BioCyc:ECOL316407_PWY0-781 aspartate superpathway
BioCyc:META_PWY-6293 superpathway of L-cysteine biosynthesis (fungi)
BioCyc:META_PWY-821 superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)
BioCyc:ECO_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
BioCyc:ECOL316407_HOMOSER-METSYN-PWY methionine biosynthesis I
BioCyc:ECOL316407_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:META_PWY-5347 superpathway of L-methionine biosynthesis (transsulfuration)
BioCyc:META_HOMOSER-METSYN-PWY L-methionine biosynthesis I
BioCyc:META_PWY-I9 L-cysteine biosynthesis VI (from L-methionine)
BioCyc:ECO_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:YEAST_PWY-821 superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)
BioCyc:MTBH37RV_PWY-I9 L-cysteine biosynthesis VI (from L-methionine)
BioCyc:MTBCDC1551_PWY-6292 superpathway of L-cysteine biosynthesis (mammalian)
BioCyc:MTBCDC1551_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:MTBCDC1551_HOMOCYSDEGR-PWY L-cysteine biosynthesis III (from L-homocysteine)
BioCyc:HPY_PWY-I9 L-cysteine biosynthesis VI (from L-methionine)
BioCyc:META_METSYN-PWY superpathway of L-homoserine and L-methionine biosynthesis
BioCyc:META_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
BioCyc:META_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:ECO_PWY0-781 aspartate superpathway
BioCyc:ECO_METSYN-PWY superpathway of L-homoserine and L-methionine biosynthesis
BioCyc:YEAST_PWY-801 homocysteine and cysteine interconversion
BioCyc:YEAST_PWY-6293 cysteine biosynthesis IV (fungi)
BioCyc:MTBCDC1551_METSYN-PWY L-homoserine and L-methionine biosynthesis
BioCyc:MTBCDC1551_HOMOSER-METSYN-PWY L-methionine biosynthesis I
PathBank:SMP0000834 Methionine Biosynthesis
Reactome:R-MTU-870392 Mycobacterium tuberculosis biological processes
Reactome:R-MTU-937250 Sulfur amino acid metabolism
Reactome:R-MTU-936621 Sulfur compound metabolism
PathBank:SMP0002371 Selenocompound Metabolism