EC: 2.4.2.8

hypoxanthine phosphoribosyltransferase (IMP:diphosphate phospho-D-ribosyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:P27605 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Guanine -> GMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: BM42B medium
PH: 7
Temperature: 37
uniprot:P27605 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Hypoxanthine -> Diphosphate + IMP		 (( (Vmax * S) ) / (Km + S)) buffer: BM42B medium
PH: 7
Temperature: 37
uniprot:P27605 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Guanine -> GMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: BM42B medium
PH: 7
Temperature: 37
uniprot:P27605 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

Guanine + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + GMP		 (( (Vmax * S) ) / (Km + S)) buffer: BM42B medium
PH: 7
Temperature: 37
uniprot:P27605 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Hypoxanthine -> IMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 1 mM K-EDTA, 5 mg/ml BSA
PH: 7.4
Temperature: 37
uniprot:P27605 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Guanine -> Diphosphate + GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 1 mM K-EDTA, 5 mg/ml BSA
PH: 7.4
Temperature: 37
uniprot:P00492 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Hypoxanthine -> IMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 12 mM MgCl2
PH: 7.4
Temperature: 30
- [hypoxanthine phosphoribosyltransferase(Enzyme) mutant chimeric DS1]

Hypoxanthine + 5_Phospho_alpha_D_ribose_1_diphosphate -> IMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 12 mM MgCl2
PH: 7.4
Temperature: 30
uniprot:P00492 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Hypoxanthine -> IMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 12 mM MgCl2
PH: 7.4
Temperature: 30
uniprot:P00492 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

GMP + Diphosphate -> Guanine + 5_Phospho_alpha_D_ribose_1_diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 12 mM MgCl2
PH: 7.4
Temperature: 30
uniprot:P00492 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

Diphosphate + GMP -> Guanine + 5_Phospho_alpha_D_ribose_1_diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 12 mM MgCl2
PH: 7.4
Temperature: 30
- [hypoxanthine phosphoribosyltransferase(Enzyme) mutant chimeric DS1]

Hypoxanthine + 5_Phospho_alpha_D_ribose_1_diphosphate -> IMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 12 mM MgCl2
PH: 7.4
Temperature: 30
- [hypoxanthine phosphoribosyltransferase(Enzyme) mutant chimeric DS1]

5_Phospho_alpha_D_ribose_1_diphosphate + Guanine -> Diphosphate + GMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 12 mM MgCl2
PH: 7.4
Temperature: 30
- [hypoxanthine phosphoribosyltransferase(Enzyme) mutant chimeric DS1]

Xanthine + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + XMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 12 mM MgCl2
PH: 7.4
Temperature: 30
- [hypoxanthine phosphoribosyltransferase(Enzyme) mutant chimeric DS1]

Guanine + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + GMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 12 mM MgCl2
PH: 7.4
Temperature: 30
uniprot:Q8R7L0 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

Hypoxanthine + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + IMP		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM Tris-HCl, 12 mM MgCl2
PH: 7.4
Temperature: 25
uniprot:Q8R7L0 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Hypoxanthine -> IMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 12 mM MgCl2
PH: 7.4
Temperature: 37
uniprot:Q8R7L0 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Hypoxanthine -> Diphosphate + IMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 12 mM MgCl2
PH: 7.4
Temperature: 50
uniprot:Q8R7L0 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Hypoxanthine -> Diphosphate + IMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 12 mM MgCl2
PH: 7.4
Temperature: 25
uniprot:Q8R7L0 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

Hypoxanthine + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + IMP		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM Tris-HCl, 12 mM MgCl2
PH: 7.4
Temperature: 37
uniprot:Q8R7L0 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Hypoxanthine -> IMP + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM Tris-HCl, 12 mM MgCl2
PH: 7.4
Temperature: 50
uniprot:Q8R7L0 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Guanine -> GMP + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM Tris-HCl, 12 mM MgCl2
PH: 7.4
Temperature: 25
uniprot:Q8R7L0 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Guanine -> GMP + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM Tris-HCl, 12 mM MgCl2
PH: 7.4
Temperature: 37
uniprot:Q8R7L0 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

Guanine + 5_Phospho_alpha_D_ribose_1_diphosphate -> GMP + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM Tris-HCl, 12 mM MgCl2
PH: 7.4
Temperature: 50
uniprot:Q8R7L0 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

Guanine + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + GMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 12 mM MgCl2
PH: 7.4
Temperature: 50
uniprot:Q8R7L0 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

Guanine + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + GMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 12 mM MgCl2
PH: 7.4
Temperature: 25
uniprot:Q8R7L0 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Guanine -> Diphosphate + GMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 12 mM MgCl2
PH: 7.4
Temperature: 37
uniprot:Q27796 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Hypoxanthine -> IMP + Diphosphate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (( (Kia * Kb) ) + (Kb * A)) ) + (Ka * B)) ) + (A * B)) ) + (( (( (Kma * B) ) * B) ) / Kib))) buffer: 100 mM Tris-HCl, 12 mM MgCl2
PH: 7.5
Temperature: 37
uniprot:Q27796 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

Guanine + 5_Phospho_alpha_D_ribose_1_diphosphate -> GMP + Diphosphate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (( (Kia * Kb) ) + (Kb * A)) ) + (Ka * B)) ) + (A * B)) ) + (( (( (Kma * B) ) * B) ) / Kib))) buffer: 100 mM Tris-HCl, 12 mM MgCl2
PH: 7.5
Temperature: 37
uniprot:Q27796 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

IMP + Diphosphate -> 5_Phospho_alpha_D_ribose_1_diphosphate + Hypoxanthine		 (( (( (Vmax * A) ) * B) ) / (( (( (( (( (Kia * Kb) ) + (Kb * A)) ) + (Ka * B)) ) + (A * B)) ) + (( (( (( (Kma * A) ) * B) ) * B) ) / (Kia * Kib)))) buffer: 100 mM Tris-HCl, 12 mM MgCl2
PH: 7.5
Temperature: 37
uniprot:Q27796 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

GMP + Diphosphate -> Guanine + 5_Phospho_alpha_D_ribose_1_diphosphate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (( (Kia * Kb) ) + (Kb * A)) ) + (Ka * B)) ) + (A * B)) ) + (( (( (( (Kma * A) ) * B) ) * B) ) / (Kia * Kib)))) buffer: 100 mM Tris-HCl, 12 mM MgCl2
PH: 7.5
Temperature: 37
uniprot:Q04178 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Hypoxanthine -> IMP + Diphosphate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (Kia * Kmb) ) + (Kmb * A)) ) + (Kma * B)) ) + (A * B))) buffer: 250 mM potassium phosphate, 24 mM MgCl2
PH: 7.4
Temperature: 38
uniprot:Q04178 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

Guanine + 5_Phospho_alpha_D_ribose_1_diphosphate -> GMP + Diphosphate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (Kia * Kmb) ) + (Kmb * A)) ) + (Kma * B)) ) + (A * B))) buffer: 250 mM potassium phosphate, 24 mM MgCl2
PH: 7.4
Temperature: 38
uniprot:Q04178 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Hypoxanthine -> Diphosphate + IMP		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris/HCl, 5 mM MgCl2
PH: 8.5
Temperature: 30
uniprot:Q04178 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

5_Phospho_alpha_D_ribose_1_diphosphate + Hypoxanthine -> Diphosphate + IMP		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris/HCl, 5 mM MgCl2
PH: 8.5
Temperature: 30
uniprot:Q04178 [hypoxanthine phosphoribosyltransferase(Enzyme) wildtype]

Guanine + 5_Phospho_alpha_D_ribose_1_diphosphate -> Diphosphate + GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 400 mM Tris/HCl, 5 mM MgCl2
PH: 8.5
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-6620 guanine and guanosine salvage
BioCyc:META_PWY66-409 superpathway of purine nucleotide salvage
BioCyc:META_PWY-6609 adenine and adenosine salvage III
BioCyc:MTBCDC1551_PWY-6620 guanine and guanosine salvage
BioCyc:BSUB_PWY-6610 adenine salvage
BioCyc:HUMAN_PWY66-409 superpathway of purine nucleotide salvage
BioCyc:ECO_PWY-6579 superpathway of guanine and guanosine salvage
BioCyc:YEAST_PWY3O-743 salvage pathways of guanine, xanthine and their nucleosides
BioCyc:MTBH37RV_PWY-6620 guanine and guanosine salvage
BioCyc:MTBH37RV_PWY-6609 adenine and adenosine salvage III
BioCyc:MTBCDC1551_PWY-6609 adenine and adenosine salvage III
BioCyc:MOUSE_P1-PWY purine and pyrimidine metabolism
BioCyc:MOUSE_PWY-6609 adenine and adenosine salvage III
BioCyc:BSUB_P1-PWY purine and pyrimidine metabolism
BioCyc:HUMAN_PWY-6620 guanine and guanosine salvage
BioCyc:META_PWY-6579 superpathway of guanine and guanosine salvage
BioCyc:META_PWY-6610 adenine salvage
BioCyc:ECO_PWY-6609 adenine and adenosine salvage III
BioCyc:YEAST_PWY-6610 adenine and adenosine salvage IV
BioCyc:YEAST_PWY3O-2220 salvage pathways of adenine, hypoxanthine and their nucleosides
BioCyc:YEAST_PWY-6620 guanine and guanosine salvage I
BioCyc:YEAST_PWY3O-1 superpathway of purine nucleosides salvage
BioCyc:MTBH37RV_PWY-6599 guanine and guanosine salvage II
BioCyc:MTBCDC1551_PWY-6599 guanine and guanosine salvage II
BioCyc:MOUSE_PWY-6599 guanine and guanosine salvage II
BioCyc:ECOL316407_PWY-6609 adenine and adenosine salvage III
BioCyc:ECOL316407_PWY-6579 superpathway of guanine and guanosine salvage
BioCyc:HUMAN_PWY-6609 adenine and adenosine salvage III
BioCyc:META_PWY-6599 guanine and guanosine salvage II
BioCyc:ECO_PWY-6620 guanine and guanosine salvage
BioCyc:BSUB_PWY-6620 guanine and guanosine salvage
BioCyc:ECOL316407_PWY-6620 guanine and guanosine salvage
Reactome:R-BTA-9748784 Drug ADME
Reactome:R-RNO-9748784 Drug ADME
Reactome:R-SSC-9748784 Drug ADME
Reactome:R-DDI-9748787 Azathioprine ADME
Reactome:R-HSA-9748787 Azathioprine ADME
Reactome:R-HSA-9734281 Defective HPRT1 disrupts guanine and hypoxanthine salvage
Reactome:R-BTA-9748787 Azathioprine ADME
Reactome:R-MMU-9748784 Drug ADME
Reactome:R-RNO-9748787 Azathioprine ADME
Reactome:R-SSC-9748787 Azathioprine ADME
Reactome:R-HSA-9734207 Nucleotide salvage defects
Reactome:R-DDI-9748784 Drug ADME
Reactome:R-HSA-9748784 Drug ADME
Reactome:R-MMU-9748787 Azathioprine ADME
Reactome:R-HSA-9735804 Diseases of nucleotide metabolism
Reactome:R-BTA-1430728 Metabolism
Reactome:R-DDI-8956321 Nucleotide salvage
Reactome:R-HSA-74217 Purine salvage
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-74217 Purine salvage
Reactome:R-RNO-74217 Purine salvage
Reactome:R-SSC-8956321 Nucleotide salvage
Reactome:R-BTA-74217 Purine salvage
Reactome:R-DDI-15869 Nucleotide metabolism
Reactome:R-HSA-8956321 Nucleotide salvage
Reactome:R-MMU-8956321 Nucleotide salvage
Reactome:R-RNO-8956321 Nucleotide salvage
Reactome:R-SSC-1430728 Metabolism
Reactome:R-SSC-15869 Nucleotide metabolism
Reactome:R-BTA-15869 Nucleotide metabolism
Reactome:R-DDI-74217 Purine salvage
Reactome:R-HSA-5668914 Diseases of metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-SSC-74217 Purine salvage
Reactome:R-BTA-8956321 Nucleotide salvage
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-RNO-15869 Nucleotide metabolism
WikiPathways:WP4792 Purine metabolism
WikiPathways:WP4224 Purine metabolism and related disorders
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0000609 Mercaptopurine Metabolism Pathway
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000427 Azathioprine Action Pathway
PathBank:SMP0000647 Thioguanine Metabolism Pathway
PathBank:SMP0087239 Purine Metabolism