EC: 2.4.1.227

undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (UDP-N-acetyl-D-glucosamine:N-acetyl-alpha-D-muramyl(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase)

enzyme lambda metabolic reaction experiment
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) mutant N291A C-terminal His-tag]

N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP_N_acetyl_D_glucosamine -> N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) mutant R260A C-terminal His-tag]

UDP_N_acetyl_D_glucosamine + N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol -> UDP + N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) mutant N198A C-terminal His-tag]

N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP_N_acetyl_D_glucosamine -> N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) mutant E125A C-terminal His-tag]

N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP_N_acetyl_D_glucosamine -> N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) wildtype C-terminal His-tag]

N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP_N_acetyl_D_glucosamine -> N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) mutant H124A C-terminal His-tag]

UDP_N_acetyl_D_glucosamine + N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol -> N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) mutant Y105A C-terminal His-tag]

N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP_N_acetyl_D_glucosamine -> N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) mutant S191A C-terminal His-tag]

N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP_N_acetyl_D_glucosamine -> UDP + N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) mutant T15A C-terminal His-tag]

N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP_N_acetyl_D_glucosamine -> N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) mutant N291A C-terminal His-tag]

UDP_N_acetyl_D_glucosamine + N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol -> N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) mutant R260A C-terminal His-tag]

UDP_N_acetyl_D_glucosamine + N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol -> UDP + N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) mutant N198A C-terminal His-tag]

UDP_N_acetyl_D_glucosamine + N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol -> N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) mutant E125A C-terminal His-tag]

UDP_N_acetyl_D_glucosamine + N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol -> N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) wildtype C-terminal His-tag]

N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP_N_acetyl_D_glucosamine -> UDP + N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) mutant T15A C-terminal His-tag]

N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP_N_acetyl_D_glucosamine -> N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) mutant H124A C-terminal His-tag]

N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP_N_acetyl_D_glucosamine -> N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) mutant Y105A C-terminal His-tag]

N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP_N_acetyl_D_glucosamine -> UDP + N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37
uniprot:P17443 [undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase(Enzyme) mutant S191A C-terminal His-tag]

N_Acetylmuramoyl_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol + UDP_N_acetyl_D_glucosamine -> UDP + N_Acetylmuramoyl_(N_acetylglucosamine)_(N_epsilon_dansylpentapeptide)_pyrophosphoryl_(R,S)_alpha_dihydroheptaprenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 35% (v/v) dimethyl sulfoxide
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:ECO_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:BSUB_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:META_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:META_PWY-5265 peptidoglycan biosynthesis II (staphylococci)
BioCyc:VCHO_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:ANTHRA_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:META_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:MTBCDC1551_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:AGRO_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:SYNEL_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECOL316407_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:CAULONA1000_PWY-5746 peptidoglycan biosynthesis I
BioCyc:PDIF272563_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:BTHE_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:BTHE_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:MTBH37RV_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:HPY_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECOL199310_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:ECOL199310_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:CAULO_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:GCF_000013425_PWY-5265 peptidoglycan biosynthesis II (staphylococci)
WikiPathways:WP2472 Escherichia coli K-12 peripherome
PathBank:SMP0000924 Peptidoglycan Biosynthesis I
PathBank:SMP0002074 Peptidoglycan Biosynthesis II
PathBank:SMP0122239 Peptidoglycan Biosynthesis II
PathBank:SMP0121305 Peptidoglycan Biosynthesis I