EC: 2.4.1.182

lipid-A-disaccharide synthase (UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine:2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine 1-phosphate 2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosaminyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:P10441 [lipid-A-disaccharide synthase(Enzyme) wildtype]

2,3_Bis(3_hydroxytetradecanoyl)_beta_D_glucosaminyl_1_phosphate + UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine -> UDP + 2,3,2',3'_Tetrakis(3_hydroxytetradecanoyl)_D_glucosaminyl_1,6_beta__D_glucosamine_1_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM HEPES, 0.05% Triton X-100, 0.5 mg/ml fatty acid free bovine serum albumin
PH: 8
Temperature: 30
uniprot:P10441 [lipid-A-disaccharide synthase(Enzyme) wildtype]

UDP_2,3_bis(3_hydroxytetradecanoyl)glucosamine + 2,3_Bis(3_hydroxytetradecanoyl)_beta_D_glucosaminyl_1_phosphate -> 2,3,2',3'_Tetrakis(3_hydroxytetradecanoyl)_D_glucosaminyl_1,6_beta__D_glucosamine_1_phosphate + UDP		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM HEPES, 0.05% Triton X-100, 0.5 mg/ml fatty acid free bovine serum albumin
PH: 8
Temperature: 30

Pathways

pathway id name
WikiPathways:WP2472 Escherichia coli K-12 peripherome
PathBank:SMP0000851 Lipopolysaccharide Biosynthesis
PathBank:SMP0001919 Lipopolysaccharide Biosynthesis II
PathBank:SMP0002071 Lipopolysaccharide Biosynthesis III
PathBank:SMP0122112 Lipopolysaccharide Biosynthesis II
PathBank:SMP0121299 Lipopolysaccharide Biosynthesis
PathBank:SMP0122237 Lipopolysaccharide Biosynthesis III