EC: 2.3.1.286

protein acetyllysine N-acetyltransferase ([protein]-N6-acetyl-L-lysine:NAD+ N-acetyltransferase (NAD+-hydrolysing; 2''-O-acetyl-ADP-D-ribose-forming))

enzyme lambda metabolic reaction experiment
uniprot:P9WGG3 [protein acetyllysine N-acetyltransferase(Enzyme) wildtype His6-tagged]

acetylated_Acetyl_CoA_synthetase + H2O + NAD+ -> Acetyl_CoA_synthetase + O_Acetyl_ADP_ribose + Nicotinamide		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 150 mM NaCl
PH: 7.5
Temperature: 22
uniprot:P9WGG3 [protein acetyllysine N-acetyltransferase(Enzyme) wildtype His6-tagged]

acetylated_Acetyl_CoA_synthetase + NAD+ + H2O -> Acetyl_CoA_synthetase + O_Acetyl_ADP_ribose + Nicotinamide		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 150 mM NaCl
PH: 7.5
Temperature: 22
uniprot:Q8IE47 [protein acetyllysine N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

acetylated_Histone_H4_peptide + NAD+ + H2O -> ADPribose + acetylated_Histone_H4_peptide + Nicotinamide		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM phosphate
PH: 7.3
Temperature: 30
uniprot:Q8IE47 [protein acetyllysine N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

acetylated_Histone_H4_peptide + H2O + NAD+ -> deacetylated_Histone_H4_peptide + Nicotinamide + O_Acetyl_ADP_ribose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM phosphate
PH: 7.3
Temperature: 30
uniprot:Q8IE47 [protein acetyllysine N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

acetylated_p300_peptide + H2O + NAD+ -> Nicotinamide + acetylated_p300_peptide + ADPribose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM phosphate
PH: 7.3
Temperature: 30
uniprot:Q8IE47 [protein acetyllysine N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

acetylated_p300_peptide + NAD+ + H2O -> deacetylated_p300_peptide + Nicotinamide + O_Acetyl_ADP_ribose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM phosphate
PH: 7.3
Temperature: 30
uniprot:Q8IE47 [protein acetyllysine N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

acetylated_Histone_H3_peptide + NAD+ + Thionicotinamide -> thio_NAD+ + acetylated_Histone_H3_peptide + Nicotinamide		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM phosphate
PH: 7.3
Temperature: 30
uniprot:Q8IE47 [protein acetyllysine N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

acetylated_p300_peptide + Nicotinamide + NAD+ -> Nicotinamide + NAD+ + acetylated_p300_peptide		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM phosphate
PH: 7.5
Temperature: 30
uniprot:Q8IE47 [protein acetyllysine N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

acetylated_Histone_H3_peptide + NAD+ + H2O -> ADPribose + acetylated_Histone_H3_peptide + Nicotinamide		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM phosphate
PH: 7.3
Temperature: 30
uniprot:Q8IE47 [protein acetyllysine N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

acetylated_Histone_H3_peptide + NAD+ + H2O -> deacetylated_Histone_H3_peptide + Nicotinamide + O_Acetyl_ADP_ribose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM phosphate
PH: 7.3
Temperature: 30
uniprot:Q8IE47 [protein acetyllysine N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

acetylated_Histone_H3_peptide + NAD+ + Methanol -> Nicotinamide + acetylated_Histone_H3_peptide + beta_1_O_Methyl_adenosine_diphosphoribose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 8.5
Temperature: 30
uniprot:Q8IE47 [protein acetyllysine N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

acetylated_Histone_H3_peptide + H2O + NAD+ -> deacetylated_Histone_H3_peptide + Nicotinamide + O_Acetyl_ADP_ribose		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 150 mM phosphate
PH: 7.3
Temperature: 30
uniprot:Q8IE47 [protein acetyllysine N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

acetylated_Histone_H3_peptide + NAD+ + Nicotinamide -> Nicotinamide + acetylated_Histone_H3_peptide + NAD+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM phosphate
PH: 7.5
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-3502 superpathway of NAD biosynthesis in eukaryotes
BioCyc:META_PWY3O-4107 NAD salvage pathway V (PNC V cycle)
BioCyc:YEAST_PWY3O-4107 NAD salvage pathway
Reactome:R-DDI-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-DME-9861718 Regulation of pyruvate metabolism
Reactome:R-HSA-9861718 Regulation of pyruvate metabolism
Reactome:R-HSA-9856651 MITF-M-dependent gene expression
Reactome:R-HSA-9854907 Regulation of MITF-M dependent genes involved in metabolism
Reactome:R-MMU-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-MMU-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-RNO-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-CEL-9861718 Regulation of pyruvate metabolism
Reactome:R-DDI-9730414 MITF-M-regulated melanocyte development
Reactome:R-DME-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-HSA-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-MMU-9730414 MITF-M-regulated melanocyte development
Reactome:R-RNO-9730414 MITF-M-regulated melanocyte development
Reactome:R-HSA-9707616 Heme signaling
Reactome:R-HSA-9759476 Regulation of Homotypic Cell-Cell Adhesion
Reactome:R-RNO-9759476 Regulation of Homotypic Cell-Cell Adhesion
Reactome:R-CEL-9730414 MITF-M-regulated melanocyte development
Reactome:R-CEL-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-DME-9730414 MITF-M-regulated melanocyte development
Reactome:R-HSA-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-HSA-9730414 MITF-M-regulated melanocyte development
Reactome:R-MMU-9861718 Regulation of pyruvate metabolism
Reactome:R-DME-9917777 Epigenetic regulation by WDR5-containing histone modifying complexes
Reactome:R-HSA-9818564 Epigenetic regulation of gene expression by MLL3 and MLL4 complexes
Reactome:R-RNO-9917777 Epigenetic regulation by WDR5-containing histone modifying complexes
Reactome:R-HSA-9764265 Regulation of CDH1 Expression and Function
Reactome:R-RNO-9764725 Negative Regulation of CDH1 Gene Transcription
Reactome:R-DME-9841922 MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis
Reactome:R-HSA-9917777 Epigenetic regulation by WDR5-containing histone modifying complexes
Reactome:R-RNO-9841922 MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis
Reactome:R-HSA-9764274 Regulation of Expression and Function of Type I Classical Cadherins
Reactome:R-HSA-9764725 Negative Regulation of CDH1 Gene Transcription
Reactome:R-RNO-9764560 Regulation of CDH1 Gene Transcription
Reactome:R-DME-9818564 Epigenetic regulation of gene expression by MLL3 and MLL4 complexes
Reactome:R-HSA-9851695 Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes
Reactome:R-HSA-9841251 Mitochondrial unfolded protein response (UPRmt)
Reactome:R-RNO-9818564 Epigenetic regulation of gene expression by MLL3 and MLL4 complexes
Reactome:R-HSA-9909396 Circadian clock
Reactome:R-HSA-9764560 Regulation of CDH1 Gene Transcription
Reactome:R-RNO-9764274 Regulation of Expression and Function of Type I Classical Cadherins
Reactome:R-DME-9851695 Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes
Reactome:R-HSA-9841922 MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis
Reactome:R-MMU-9841251 Mitochondrial unfolded protein response (UPRmt)
Reactome:R-RNO-9851695 Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes
Reactome:R-HSA-9931509 Expression of BMAL (ARNTL), CLOCK, and NPAS2
Reactome:R-RNO-9764265 Regulation of CDH1 Expression and Function
Reactome:R-CEL-5693607 Processing of DNA double-strand break ends
Reactome:R-CEL-9614085 FOXO-mediated transcription
Reactome:R-HSA-9614085 FOXO-mediated transcription
Reactome:R-CEL-5693567 HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA)
Reactome:R-DME-9617629 Regulation of FOXO transcriptional activity by acetylation
Reactome:R-DME-9614085 FOXO-mediated transcription
Reactome:R-HSA-9615017 FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes
Reactome:R-CEL-5693538 Homology Directed Repair
Reactome:R-CEL-9617629 Regulation of FOXO transcriptional activity by acetylation
Reactome:R-HSA-9617629 Regulation of FOXO transcriptional activity by acetylation
Reactome:R-CEL-1852241 Organelle biogenesis and maintenance
Reactome:R-CEL-73894 DNA Repair
Reactome:R-CEL-204174 Regulation of pyruvate dehydrogenase (PDH) complex
Reactome:R-DRE-68886 M Phase
Reactome:R-DRE-2555396 Mitotic Metaphase and Anaphase
Reactome:R-DRE-2995410 Nuclear Envelope (NE) Reassembly
Reactome:R-DDI-1592230 Mitochondrial biogenesis
Reactome:R-DME-69278 Cell Cycle, Mitotic
Reactome:R-DME-2995410 Nuclear Envelope (NE) Reassembly
Reactome:R-DME-1266738 Developmental Biology
Reactome:R-DME-5250941 Negative epigenetic regulation of rRNA expression
Reactome:R-DME-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-HSA-418990 Adherens junctions interactions
Reactome:R-HSA-204174 Regulation of pyruvate dehydrogenase (PDH) complex
Reactome:R-HSA-5693532 DNA Double-Strand Break Repair
Reactome:R-HSA-5693538 Homology Directed Repair
Reactome:R-HSA-157118 Signaling by NOTCH
Reactome:R-HSA-2555396 Mitotic Metaphase and Anaphase
Reactome:R-HSA-2995410 Nuclear Envelope (NE) Reassembly
Reactome:R-HSA-74160 Gene expression (Transcription)
Reactome:R-HSA-73857 RNA Polymerase II Transcription
Reactome:R-MMU-1640170 Cell Cycle
Reactome:R-MMU-68882 Mitotic Anaphase
Reactome:R-MMU-5693607 Processing of DNA double-strand break ends
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-204174 Regulation of pyruvate dehydrogenase (PDH) complex
Reactome:R-RNO-68886 M Phase
Reactome:R-RNO-427359 SIRT1 negatively regulates rRNA expression
Reactome:R-RNO-421270 Cell-cell junction organization
Reactome:R-RNO-1266738 Developmental Biology
Reactome:R-SCE-8953897 Cellular responses to stimuli
Reactome:R-SCE-3371556 Cellular response to heat stress
Reactome:R-SCE-3371453 Regulation of HSF1-mediated heat shock response
Reactome:R-SPO-3371453 Regulation of HSF1-mediated heat shock response
Reactome:R-CEL-70268 Pyruvate metabolism
Reactome:R-CEL-1266738 Developmental Biology
Reactome:R-CEL-212436 Generic Transcription Pathway
Reactome:R-DDI-1852241 Organelle biogenesis and maintenance
Reactome:R-DDI-1266738 Developmental Biology
Reactome:R-DME-2151201 Transcriptional activation of mitochondrial biogenesis
Reactome:R-DME-68886 M Phase
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-204174 Regulation of pyruvate dehydrogenase (PDH) complex
Reactome:R-HSA-1592230 Mitochondrial biogenesis
Reactome:R-HSA-1266738 Developmental Biology
Reactome:R-HSA-421270 Cell-cell junction organization
Reactome:R-HSA-70268 Pyruvate metabolism
Reactome:R-HSA-2262752 Cellular responses to stress
Reactome:R-HSA-1640170 Cell Cycle
Reactome:R-HSA-212165 Epigenetic regulation of gene expression
Reactome:R-MMU-2995383 Initiation of Nuclear Envelope (NE) Reformation
Reactome:R-MMU-5693567 HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA)
Reactome:R-MMU-70268 Pyruvate metabolism
Reactome:R-MMU-2151201 Transcriptional activation of mitochondrial biogenesis
Reactome:R-RNO-1640170 Cell Cycle
Reactome:R-RNO-68882 Mitotic Anaphase
Reactome:R-RNO-5250941 Negative epigenetic regulation of rRNA expression
Reactome:R-RNO-446728 Cell junction organization
Reactome:R-RNO-8953897 Cellular responses to stimuli
Reactome:R-RNO-2262752 Cellular responses to stress
Reactome:R-SPO-2262752 Cellular responses to stress
Reactome:R-CEL-1592230 Mitochondrial biogenesis
Reactome:R-CEL-5693532 DNA Double-Strand Break Repair
Reactome:R-CEL-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-CEL-74160 Gene expression (Transcription)
Reactome:R-DRE-69278 Cell Cycle, Mitotic
Reactome:R-DRE-2995383 Initiation of Nuclear Envelope (NE) Reformation
Reactome:R-DDI-2151201 Transcriptional activation of mitochondrial biogenesis
Reactome:R-DME-1852241 Organelle biogenesis and maintenance
Reactome:R-DME-2555396 Mitotic Metaphase and Anaphase
Reactome:R-DME-2995383 Initiation of Nuclear Envelope (NE) Reformation
Reactome:R-DME-74160 Gene expression (Transcription)
Reactome:R-DME-73857 RNA Polymerase II Transcription
Reactome:R-DME-212165 Epigenetic regulation of gene expression
Reactome:R-DME-427359 SIRT1 negatively regulates rRNA expression
Reactome:R-HSA-2151201 Transcriptional activation of mitochondrial biogenesis
Reactome:R-HSA-1500931 Cell-Cell communication
Reactome:R-HSA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-HSA-71403 Citric acid cycle (TCA cycle)
Reactome:R-HSA-5693567 HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA)
Reactome:R-HSA-1912422 Pre-NOTCH Expression and Processing
Reactome:R-HSA-69278 Cell Cycle, Mitotic
Reactome:R-HSA-2995383 Initiation of Nuclear Envelope (NE) Reformation
Reactome:R-HSA-212436 Generic Transcription Pathway
Reactome:R-HSA-5250941 Negative epigenetic regulation of rRNA expression
Reactome:R-MMU-68886 M Phase
Reactome:R-MMU-73894 DNA Repair
Reactome:R-MMU-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-MMU-71403 Citric acid cycle (TCA cycle)
Reactome:R-MMU-1266738 Developmental Biology
Reactome:R-MMU-3371556 Cellular response to heat stress
Reactome:R-MMU-3371453 Regulation of HSF1-mediated heat shock response
Reactome:R-MMU-1852241 Organelle biogenesis and maintenance
Reactome:R-RNO-69278 Cell Cycle, Mitotic
Reactome:R-RNO-2555396 Mitotic Metaphase and Anaphase
Reactome:R-RNO-2995410 Nuclear Envelope (NE) Reassembly
Reactome:R-RNO-74160 Gene expression (Transcription)
Reactome:R-RNO-418990 Adherens junctions interactions
Reactome:R-SPO-8953897 Cellular responses to stimuli
Reactome:R-SPO-3371556 Cellular response to heat stress
Reactome:R-CEL-2151201 Transcriptional activation of mitochondrial biogenesis
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-73857 RNA Polymerase II Transcription
Reactome:R-DRE-1640170 Cell Cycle
Reactome:R-DRE-68882 Mitotic Anaphase
Reactome:R-DME-1592230 Mitochondrial biogenesis
Reactome:R-DME-1640170 Cell Cycle
Reactome:R-DME-68882 Mitotic Anaphase
Reactome:R-DME-212436 Generic Transcription Pathway
Reactome:R-DME-70268 Pyruvate metabolism
Reactome:R-HSA-1852241 Organelle biogenesis and maintenance
Reactome:R-HSA-446728 Cell junction organization
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-73894 DNA Repair
Reactome:R-HSA-5693607 Processing of DNA double-strand break ends
Reactome:R-HSA-162582 Signaling Pathways
Reactome:R-HSA-1912408 Pre-NOTCH Transcription and Translation
Reactome:R-HSA-8953897 Cellular responses to stimuli
Reactome:R-HSA-3371556 Cellular response to heat stress
Reactome:R-HSA-3371453 Regulation of HSF1-mediated heat shock response
Reactome:R-HSA-68886 M Phase
Reactome:R-HSA-68882 Mitotic Anaphase
Reactome:R-HSA-427359 SIRT1 negatively regulates rRNA expression
Reactome:R-MMU-69278 Cell Cycle, Mitotic
Reactome:R-MMU-2555396 Mitotic Metaphase and Anaphase
Reactome:R-MMU-2995410 Nuclear Envelope (NE) Reassembly
Reactome:R-MMU-5693532 DNA Double-Strand Break Repair
Reactome:R-MMU-5693538 Homology Directed Repair
Reactome:R-MMU-8953897 Cellular responses to stimuli
Reactome:R-MMU-2262752 Cellular responses to stress
Reactome:R-MMU-1592230 Mitochondrial biogenesis
Reactome:R-RNO-2995383 Initiation of Nuclear Envelope (NE) Reformation
Reactome:R-RNO-212165 Epigenetic regulation of gene expression
Reactome:R-RNO-1500931 Cell-Cell communication
Reactome:R-RNO-3371556 Cellular response to heat stress
Reactome:R-RNO-3371453 Regulation of HSF1-mediated heat shock response
Reactome:R-SCE-2262752 Cellular responses to stress
WikiPathways:WP1984 Integrated breast cancer pathway
PathBank:SMP0083297 NAD+ Signalling Pathway (Cancer)
PathBank:SMP0084271 NAD+ Signalling and Aging
PathBank:SMP0002338 NAD Metabolism
PathBank:SMP0120941 Succinate Signalling
PathBank:SMP0120965 Succinate Signalling
PathBank:SMP0083294 Succinate Signalling