EC: 2.3.1.135

phosphatidylcholine-retinol O-acyltransferase (phosphatidylcholine:retinol---[cellular-retinol-binding-protein] O-acyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q9BGL2 [phosphatidylcholine---retinol O-acyltransferase(Enzyme) wildtype]

RPE65 + RPE65 + Retinol -> All_trans_Retinyl_palmitate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM TrisHCl, 0.3% CHAPS, 1 mM dithiothreitol
PH: 8.4
Temperature: 23
uniprot:Q9JI60 [phosphatidylcholine---retinol O-acyltransferase(Enzyme) wildtype]

Retinol_(cellular_retinol_binding_protein) + 1,2_Diheptanoyl_sn_glycero_3_phosphocholine -> Retinyl_heptanoate_(cellular_retinol_binding_protein) + 2_Heptanoyl_sn_glycero_3_phosphocholine		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl, 1 mM DTT, 1% bovine serum albumin
PH: 7.5
Temperature: 30
uniprot:Q9JI60 [phosphatidylcholine---retinol O-acyltransferase(Enzyme) mutant del1-30]

Retinol_(cellular_retinol_binding_protein) + 1,2_Diheptanoyl_sn_glycero_3_phosphocholine -> Retinyl_heptanoate_(cellular_retinol_binding_protein) + 2_Heptanoyl_sn_glycero_3_phosphocholine		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl, 1 mM DTT, 1% bovine serum albumin
PH: 7.5
Temperature: 30
uniprot:Q9JI60 [phosphatidylcholine---retinol O-acyltransferase(Enzyme) mutant E14L]

Retinol_(cellular_retinol_binding_protein) + 1,2_Diheptanoyl_sn_glycero_3_phosphocholine -> Retinyl_heptanoate_(cellular_retinol_binding_protein) + 2_Heptanoyl_sn_glycero_3_phosphocholine		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl, 1 mM DTT, 1% bovine serum albumin
PH: 7.5
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-6861 the visual cycle I (vertebrates)
BioCyc:META_PWY-6857 retinol biosynthesis
BioCyc:HUMAN_PWY-6857 retinol biosynthesis
BioCyc:HUMAN_PWY-6861 the visual cycle I (vertebrates)
Reactome:R-HSA-9675143 Diseases of the neuronal system
Reactome:R-HSA-9709957 Sensory Perception
Reactome:R-MMU-9709957 Sensory Perception
Reactome:R-RNO-9709957 Sensory Perception
Reactome:R-HSA-9918442 Defective visual phototransduction due to LRAT loss of function
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-2453902 The canonical retinoid cycle in rods (twilight vision)
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-975634 Retinoid metabolism and transport
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-975634 Retinoid metabolism and transport
Reactome:R-MMU-2187338 Visual phototransduction
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-975634 Retinoid metabolism and transport
Reactome:R-HSA-6806667 Metabolism of fat-soluble vitamins
Reactome:R-HSA-2187338 Visual phototransduction
Reactome:R-HSA-2474795 Diseases associated with visual transduction
Reactome:R-MMU-6806667 Metabolism of fat-soluble vitamins
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-6806667 Metabolism of fat-soluble vitamins
Reactome:R-RNO-2187338 Visual phototransduction
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2453902 The canonical retinoid cycle in rods (twilight vision)
Reactome:R-HSA-2453864 Retinoid cycle disease events
Reactome:R-RNO-2453902 The canonical retinoid cycle in rods (twilight vision)
WikiPathways:WP5188 Retinol metabolism
WikiPathways:WP4627 Lipids measured in liver metastasis from breast cancer
PathBank:SMP0087353 Retinol Metabolism
PathBank:SMP0120720 Vitamin A Deficiency
PathBank:SMP0000074 Retinol Metabolism
PathBank:SMP0000336 Vitamin A Deficiency
PathBank:SMP0087260 Retinol Metabolism