EC: 2.1.2.9

methionyl-tRNA formyltransferase (10-formyltetrahydrofolate:L-methionyl-tRNA N-formyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q82ZD8 [methionyl-tRNA formyltransferase(Enzyme) wildtype]

L_Methionyl_tRNA(Met) + 10_Formyltetrahydrofolate -> 5,6,7,8_Tetrahydrofolate + N_Formylmethionyl_tRNA(fMet)		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 mM Tris-chloride, 30 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.25
Temperature: 30
uniprot:Q82ZD8 [methionyl-tRNA formyltransferase(Enzyme) wildtype]

L_Methionyl_tRNA(Met) + 10_Formyltetrahydrofolate -> 5,6,7,8_Tetrahydrofolate + N_Formylmethionyl_tRNA(fMet)		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 mM Tris-chloride, 30 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.25
Temperature: 30
uniprot:Q82ZD8 [methionyl-tRNA formyltransferase(Enzyme) wildtype]

10_Formyltetrahydrofolate + L_Methionyl_tRNA(Met) -> N_Formylmethionyl_tRNA(fMet) + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 mM Tris-chloride, 30 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.25
Temperature: 30
uniprot:Q82ZD8 [methionyl-tRNA formyltransferase(Enzyme) wildtype]

10_Formyltetrahydrofolyl_[Glu](3) + L_Methionyl_tRNA(Met) -> N_Formylmethionyl_tRNA(fMet) + Tetrahydrofolyl_[Glu](3)		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 mM Tris-chloride, 30 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.25
Temperature: 30
uniprot:Q82ZD8 [methionyl-tRNA formyltransferase(Enzyme) wildtype]

L_Methionyl_tRNA(Met) + 10_Formyltetrahydrofolyl_[Glu](3) -> N_Formylmethionyl_tRNA(fMet) + Tetrahydrofolyl_[Glu](3)		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 mM Tris-chloride, 30 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.25
Temperature: 30
uniprot:Q82ZD8 [methionyl-tRNA formyltransferase(Enzyme) wildtype]

10_Formyltetrahydrofolate + L_Methionyl_tRNA(Met) -> N_Formylmethionyl_tRNA(fMet) + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 mM Tris-chloride, 30 mM MgCl2, 20 mM 2-mercaptoethanol
PH: 7.25
Temperature: 30

Pathways

pathway id name
PathBank:SMP0001750 One Carbon Pool by Folate
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-5368286 Mitochondrial translation initiation
Reactome:R-HSA-5368287 Mitochondrial translation
Reactome:R-HSA-72766 Translation
PathBank:SMP0120718 Hypermethioninemia
PathBank:SMP0120742 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0122040 One Carbon Pool by Folate
PathBank:SMP0000432 Methotrexate Action Pathway
PathBank:SMP0087342 Methionine Metabolism
PathBank:SMP0120853 Homocystinuria-Megaloblastic Anemia Due to Defect in Cobalamin Metabolism, cblG Complementation Type
PathBank:SMP0120471 Cystathionine beta-Synthase Deficiency
PathBank:SMP0120487 Glycine N-Methyltransferase Deficiency
PathBank:SMP0120519 Methionine Adenosyltransferase Deficiency
PathBank:SMP0120527 S-Adenosylhomocysteine (SAH) Hydrolase Deficiency
PathBank:SMP0000543 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0087196 Folate Metabolism
PathBank:SMP0087249 Methionine Metabolism
PathBank:SMP0063637 Methionine Metabolism
PathBank:SMP0120656 Folate Malabsorption, Hereditary
PathBank:SMP0120634 Homocystinuria-Megaloblastic Anemia Due to Defect in Cobalamin Metabolism, cblG Complementation Type
PathBank:SMP0120691 Cystathionine beta-Synthase Deficiency
PathBank:SMP0120707 Glycine N-Methyltransferase Deficiency
PathBank:SMP0120739 Methionine Adenosyltransferase Deficiency
PathBank:SMP0120747 S-Adenosylhomocysteine (SAH) Hydrolase Deficiency
PathBank:SMP0120875 Folate Malabsorption, Hereditary
PathBank:SMP0120498 Hypermethioninemia
PathBank:SMP0120522 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0000053 Folate Metabolism
PathBank:SMP0000724 Folate Malabsorption, Hereditary
PathBank:SMP0000801 Folate Metabolism
PathBank:SMP0087295 Folate Metabolism