EC: 1.8.1.7

glutathione-disulfide reductase (glutathione:NADP+ oxidoreductase)

enzyme lambda metabolic reaction experiment
- [glutathione-disulfide reductase(Enzyme) wildtype]

Oxidized_glutathione + H+ + NADPH -> 2 Reduced_glutathione + NADP+		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (Km_A * B) ) + (Km_B * A)) ) + (A * B))) buffer: 0.1 M Potassium phosphate, 1 mM EDTA
PH: 7
Temperature: 30
uniprot:D3RSV1 [FAD]

Oxidized_glutathione + H+ + NADH -> 2 Reduced_glutathione + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.25 M Potassium phosphate
PH: 7
Temperature: 36
uniprot:D3RSV1 [FAD]

NADH + H+ + Oxidized_glutathione -> 2 Reduced_glutathione + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.25 M HEPES
PH: 7
Temperature: 36
uniprot:D3RSV1 [FAD]

Oxidized_glutathione + NADH + H+ -> NAD+ + 2 Reduced_glutathione		 (( (Vmax * A) ) / (Km + A)) buffer: 0.25 M Potassium phosphate, 0.005% 5,5-Dithio-bis-2-nitrobenzoate
PH: 7
Temperature: 36
uniprot:D3RSV1 [FAD]

NADH + H+ + Oxidized_glutathione -> 2 Reduced_glutathione + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.25 M HEPES
PH: 7
Temperature: 36
uniprot:P70619 [glutathione-disulfide reductase(Enzyme) wildtype]

H+ + NADPH + Oxidized_glutathione -> 2 Reduced_glutathione + NADP+		 (( (E * (( (( (kcat1 * A) ) * B) ) + (( (kcat2 * (A ^ 2)) ) * B))) ) / (( (( (( (( (K1 * A) ) + (K2 * B)) ) + (A * B)) ) + (K3 * (A ^ 2))) ) + (( (K4 * (A ^ 2)) ) * B))) buffer: 0.10 M Sodium phosphate, 0.5 mM EDTA
PH: 7.6
Temperature: 30
uniprot:P70619 [glutathione-disulfide reductase(Enzyme) wildtype]

H+ + Oxidized_glutathione + NADPH -> 2 Reduced_glutathione + NADP+		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (Ka * B) ) + (Kb * A)) ) + (A * B))) buffer: 0.10 M Sodium phosphate, 0.5 mM EDTA
PH: 7.6
Temperature: 30
uniprot:Q6UK62 [glutathione-disulfide reductase(Enzyme) wildtype]

Oxidized_glutathione + H+ + NADPH -> 2 Reduced_glutathione + NADP+		 (( (( (V * A) ) * B) ) / (( (( (KmA * B) ) + (KmB * A)) ) + (A * B))) buffer: potassium phosphate, 0.2 M KCl, 1 mM EDTA
PH: 7
Temperature: 30
uniprot:Q6UK62 [glutathione-disulfide reductase(Enzyme) wildtype]

NADPH + H+ + Oxidized_glutathione -> NADP+ + 2 Reduced_glutathione		 (( (( (V * A) ) * B) ) / (( (( (KmA * B) ) + (KmB * A)) ) + (A * B))) buffer: potassium phosphate, 0.2 M KCl, 1 mM EDTA
PH: 7
Temperature: 30
- [Rhein]

H+ + Oxidized_glutathione + NADPH -> NADP+ + 2 Reduced_glutathione		 (( (V * A) ) / (K + (A * (1 + (I / Kii))))) buffer: 50 mM K2HPO4, 0,1 mM EDTA
PH: 7.5
Temperature: 25
- [glutathione-disulfide reductase(Enzyme) wildtype]

NADPH + Oxidized_glutathione + H+ -> NADP+ + 2 Reduced_glutathione		 (( (V * A) ) / (( (K * (1 + (I / Kis))) ) + A)) buffer: 50 mM K2HPO4, 0,1 mM EDTA
PH: 7.5
Temperature: 25
- [glutathione-disulfide reductase(Enzyme) wildtype]

Oxidized_glutathione + H+ + NADPH -> 2 Reduced_glutathione + NADP+		 (( (( (V * A) ) * B) ) / (( (( (KA * B) ) + (KB * A)) ) + (A * B))) buffer: 50 mM K2HPO4, 0,1 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q99MD6 [FAD]

NADPH + Oxidized_glutathione + H+ -> NADP+ + 2 Reduced_glutathione		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 0.5 mM EDTA
PH: 7.5
Temperature: 25
uniprot:P70619 [glutathione-disulfide reductase(Enzyme) wildtype]

H+ + Oxidized_glutathione + NADPH -> 2 Reduced_glutathione + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100
PH: 7.2
Temperature: 37
uniprot:P70619 [glutathione-disulfide reductase(Enzyme) wildtype]

Oxidized_glutathione + NADPH + H+ -> 2 Reduced_glutathione + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100
PH: 7.2
Temperature: 37
uniprot:P70619 [glutathione-disulfide reductase(Enzyme) wildtype]

Oxidized_glutathione + H+ + NADPH -> 2 Reduced_glutathione + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100
PH: 7.2
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-4081 glutathione-peroxide redox reactions
BioCyc:YEAST_PWY-4081 glutathione redox reactions I
BioCyc:ARA_PWY-4081 glutathione-peroxide redox reactions
BioCyc:MOUSE_PWY-4081 glutathione redox reactions I
BioCyc:HUMAN_PWY-4081 glutathione redox reactions I
PathBank:SMP0000853 Glutathione Metabolism
PathBank:SMP0001941 Glutathione Metabolism II
PathBank:SMP0002032 Glutathione Metabolism III
PlantCyc:ARA_PWY-4081 glutathione-peroxide redox reactions
Reactome:R-RNO-9711123 Cellular response to chemical stress
Reactome:R-SPO-9711123 Cellular response to chemical stress
Reactome:R-CEL-9711123 Cellular response to chemical stress
Reactome:R-DDI-9711123 Cellular response to chemical stress
Reactome:R-SCE-9711123 Cellular response to chemical stress
Reactome:R-MMU-9711123 Cellular response to chemical stress
Reactome:R-CEL-8953897 Cellular responses to stimuli
Reactome:R-CEL-3700989 Transcriptional Regulation by TP53
Reactome:R-DDI-3299685 Detoxification of Reactive Oxygen Species
Reactome:R-DDI-5628897 TP53 Regulates Metabolic Genes
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-3299685 Detoxification of Reactive Oxygen Species
Reactome:R-MMU-74160 Gene expression (Transcription)
Reactome:R-MMU-73857 RNA Polymerase II Transcription
Reactome:R-MMU-3700989 Transcriptional Regulation by TP53
Reactome:R-SCE-3700989 Transcriptional Regulation by TP53
Reactome:R-SCE-8953897 Cellular responses to stimuli
Reactome:R-SPO-3700989 Transcriptional Regulation by TP53
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-15869 Nucleotide metabolism
Reactome:R-SPO-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-SPO-3299685 Detoxification of Reactive Oxygen Species
Reactome:R-CEL-3299685 Detoxification of Reactive Oxygen Species
Reactome:R-CEL-212436 Generic Transcription Pathway
Reactome:R-DDI-2262752 Cellular responses to stress
Reactome:R-DDI-15869 Nucleotide metabolism
Reactome:R-DDI-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-DDI-3700989 Transcriptional Regulation by TP53
Reactome:R-RNO-8953897 Cellular responses to stimuli
Reactome:R-RNO-2262752 Cellular responses to stress
Reactome:R-SCE-212436 Generic Transcription Pathway
Reactome:R-SCE-3299685 Detoxification of Reactive Oxygen Species
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SPO-212436 Generic Transcription Pathway
Reactome:R-SPO-2262752 Cellular responses to stress
Reactome:R-CEL-15869 Nucleotide metabolism
Reactome:R-CEL-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-CEL-74160 Gene expression (Transcription)
Reactome:R-CEL-5628897 TP53 Regulates Metabolic Genes
Reactome:R-DDI-8953897 Cellular responses to stimuli
Reactome:R-DDI-74160 Gene expression (Transcription)
Reactome:R-DDI-73857 RNA Polymerase II Transcription
Reactome:R-MMU-212436 Generic Transcription Pathway
Reactome:R-MMU-5628897 TP53 Regulates Metabolic Genes
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-74160 Gene expression (Transcription)
Reactome:R-RNO-73857 RNA Polymerase II Transcription
Reactome:R-RNO-3700989 Transcriptional Regulation by TP53
Reactome:R-RNO-3299685 Detoxification of Reactive Oxygen Species
Reactome:R-SCE-5628897 TP53 Regulates Metabolic Genes
Reactome:R-SCE-15869 Nucleotide metabolism
Reactome:R-SCE-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-SPO-74160 Gene expression (Transcription)
Reactome:R-SPO-5628897 TP53 Regulates Metabolic Genes
Reactome:R-SPO-8953897 Cellular responses to stimuli
Reactome:R-CEL-2262752 Cellular responses to stress
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-73857 RNA Polymerase II Transcription
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-212436 Generic Transcription Pathway
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-MMU-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-MMU-8953897 Cellular responses to stimuli
Reactome:R-MMU-2262752 Cellular responses to stress
Reactome:R-RNO-15869 Nucleotide metabolism
Reactome:R-RNO-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-RNO-212436 Generic Transcription Pathway
Reactome:R-RNO-5628897 TP53 Regulates Metabolic Genes
Reactome:R-SCE-74160 Gene expression (Transcription)
Reactome:R-SCE-73857 RNA Polymerase II Transcription
Reactome:R-SCE-2262752 Cellular responses to stress
Reactome:R-SPO-73857 RNA Polymerase II Transcription
PathBank:SMP0120484 Glutaric Aciduria Type I
PathBank:SMP0120593 Hyperlysinemia II or Saccharopinuria
PathBank:SMP0120613 2-Ketoglutarate Dehydrogenase Complex Deficiency
PathBank:SMP0000015 Glutathione Metabolism
PathBank:SMP0000339 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0000500 5-Oxoprolinase Deficiency
PathBank:SMP0063579 Ammonia Recycling
PathBank:SMP0120653 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0108760 Glutaminolysis and Cancer
PathBank:SMP0120475 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0120592 Hyperlysinemia I, Familial
PathBank:SMP0120612 Mitochondrial Complex II Deficiency
PathBank:SMP0000385 Homocarnosinosis
PathBank:SMP0000136 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0000567 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0108758 The Oncogenic Action of 2-Hydroxyglutarate
PathBank:SMP0120521 Sarcosinemia
PathBank:SMP0120610 Congenital Lactic Acidosis
PathBank:SMP0120614 Pyruvate Dehydrogenase Deficiency (E3)
PathBank:SMP0000337 Glutathione Synthetase Deficiency
PathBank:SMP0000501 gamma-Glutamyltranspeptidase Deficiency
PathBank:SMP0120651 2-Aminoadipic 2-Oxoadipic Aciduria
PathBank:SMP0120474 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0120526 Saccharopinuria/Hyperlysinemia II
PathBank:SMP0120534 Non-Ketotic Hyperglycinemia
PathBank:SMP0120550 Hyperglycinemia, Non-Ketotic
PathBank:SMP0120611 Fumarase Deficiency
PathBank:SMP0120615 Pyruvate Dehydrogenase Deficiency (E2)
PathBank:SMP0120635 Pyridoxine Dependency with Seizures
PathBank:SMP0000072 Glutamate Metabolism
PathBank:SMP0000243 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000143 5-Oxoprolinuria
PathBank:SMP0000183 gamma-Glutamyltransferase Deficiency
PathBank:SMP0002304 TCA Cycle
PathBank:SMP0002355 Pyruvate Metabolism
PathBank:SMP0086930 Warburg Effect
PathBank:SMP0063477 Citric Acid Cycle
PathBank:SMP0063626 Glycine and Serine Metabolism
PathBank:SMP0063635 Lysine Degradation