EC: 1.4.3.3

D-amino-acid oxidase (D-amino-acid:oxygen oxidoreductase (deaminating))

enzyme lambda metabolic reaction experiment
uniprot:P00371 [FAD]

H2O + (R)_1_Phenylethylamine + O2 -> H2O2 + Acetophenone + NH3		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM CHES NaOH
PH: 8.6
Temperature: 25
uniprot:P00371 [FAD]

H2O + (R)_1_Phenylethylamine + O2 -> H2O2 + Acetophenone + NH3		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 200 mM CHES NaOH
PH: 8.6
Temperature: 25
uniprot:P14920 [FAD]

O2 + D_Alanine + H2O -> Pyruvate + H2O2 + NH3		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 40 mM sodium pyrophosphate (air-saturated), 153 U/ml Aspergillus niger catalase
PH: 8.3
Temperature: 37
uniprot:Q95XG9 [FAD]

D_Alanine + O2 + H2O -> Pyruvate + H2O2 + NH3		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 40 mM sodium pyrophosphate (air-saturated), 153 U/ml Aspergillus niger catalase
PH: 8.3
Temperature: 37
uniprot:P14920 [FAD]

H2O + D_Arginine + O2 -> NH3 + H2O2 + 5_Guanidino_2_oxopentanoate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 40 mM sodium pyrophosphate (air-saturated), 153 U/ml Aspergillus niger catalase
PH: 8.3
Temperature: 37
uniprot:Q95XG9 [FAD]

H2O + D_Arginine + O2 -> 5_Guanidino_2_oxopentanoate + H2O2 + NH3		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 40 mM sodium pyrophosphate (air-saturated), 153 U/ml Aspergillus niger catalase
PH: 8.3
Temperature: 37
uniprot:P14920 [FAD]

O2 + H2O + D_Phenylalanine -> Phenylpyruvate + NH3 + H2O2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 40 mM sodium pyrophosphate (air-saturated), 153 U/ml Aspergillus niger catalase
PH: 8.3
Temperature: 37
uniprot:Q95XG9 [FAD]

D_Phenylalanine + O2 + H2O -> Phenylpyruvate + H2O2 + NH3		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 40 mM sodium pyrophosphate (air-saturated), 153 U/ml Aspergillus niger catalase
PH: 8.3
Temperature: 37
uniprot:P14920 [FAD]

O2 + D_Histidine + H2O -> 1H_Imidazol_4_yl(oxo)acetic_acid + H2O2 + NH3		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 40 mM sodium pyrophosphate (air-saturated), 153 U/ml Aspergillus niger catalase
PH: 8.3
Temperature: 37
uniprot:Q95XG9 [FAD]

D_Histidine + H2O + O2 -> 1H_Imidazol_4_yl(oxo)acetic_acid + NH3 + H2O2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 40 mM sodium pyrophosphate (air-saturated), 153 U/ml Aspergillus niger catalase
PH: 8.3
Temperature: 37
uniprot:P14920 [FAD]

H2O + D_Serine + O2 -> 3_Hydroxypyruvate + NH3 + H2O2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 40 mM sodium pyrophosphate (air-saturated), 153 U/ml Aspergillus niger catalase
PH: 8.3
Temperature: 37
uniprot:Q95XG9 [FAD]

D_Serine + O2 + H2O -> 3_Hydroxypyruvate + H2O2 + NH3		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 40 mM sodium pyrophosphate (air-saturated), 153 U/ml Aspergillus niger catalase
PH: 8.3
Temperature: 37
uniprot:Q6TGN2 [D-amino-acid oxidase(Enzyme) wildtype]

O2 + H2O + D_Alanine -> H2O2 + Pyruvate + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium pyrophosphate, 5 µg/ml FAD, 50 µg/ml BSA, 180 µg/ml Peroxidase, 750 µg/ml sodium 3, 5-dichloro-2-hydroxybenzenesulphonic acid, 112.5 µg/ml 4-aminoantipyrine, 7.5 mM sodium azide
PH: 8
Temperature: 37
uniprot:Q6TGN2 [D-amino-acid oxidase(Enzyme) wildtype]

O2 + H2O + D_Phenylalanine -> NH3 + Phenylpyruvate + H2O2		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium pyrophosphate, 5 µg/ml FAD, 50 µg/ml BSA, 180 µg/ml Peroxidase, 750 µg/ml sodium 3, 5-dichloro-2-hydroxybenzenesulphonic acid, 112.5 µg/ml 4-aminoantipyrine, 7.5 mM sodium azide
PH: 8
Temperature: 37
uniprot:Q6TGN2 [D-amino-acid oxidase(Enzyme) wildtype]

D_Isoleucine + O2 + H2O -> 3_Methyl_2_oxopentanoate + NH3 + H2O2		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium pyrophosphate, 5 µg/ml FAD, 50 µg/ml BSA, 180 µg/ml Peroxidase, 750 µg/ml sodium 3, 5-dichloro-2-hydroxybenzenesulphonic acid, 112.5 µg/ml 4-aminoantipyrine, 7.5 mM sodium azide
PH: 8
Temperature: 37
uniprot:Q6TGN2 [D-amino-acid oxidase(Enzyme) wildtype]

H2O + O2 + D_Valine -> NH3 + alpha_Ketovaline + H2O2		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium pyrophosphate, 5 µg/ml FAD, 50 µg/ml BSA, 180 µg/ml Peroxidase, 750 µg/ml sodium 3, 5-dichloro-2-hydroxybenzenesulphonic acid, 112.5 µg/ml 4-aminoantipyrine, 7.5 mM sodium azide
PH: 8
Temperature: 37
uniprot:Q6TGN2 [D-amino-acid oxidase(Enzyme) wildtype]

O2 + D_Proline + H2O -> NH3 + H2O2 + 2_Oxopentanoate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium pyrophosphate, 5 µg/ml FAD, 50 µg/ml BSA, 180 µg/ml Peroxidase, 750 µg/ml sodium 3, 5-dichloro-2-hydroxybenzenesulphonic acid, 112.5 µg/ml 4-aminoantipyrine, 7.5 mM sodium azide
PH: 8
Temperature: 37
uniprot:Q6TGN2 [D-amino-acid oxidase(Enzyme) wildtype]

D_Tyrosine + O2 + H2O -> NH3 + 3_(4_Hydroxyphenyl)pyruvate + H2O2		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium pyrophosphate, 5 µg/ml FAD, 50 µg/ml BSA, 180 µg/ml Peroxidase, 750 µg/ml sodium 3, 5-dichloro-2-hydroxybenzenesulphonic acid, 112.5 µg/ml 4-aminoantipyrine, 7.5 mM sodium azide
PH: 8
Temperature: 37
uniprot:Q6TGN2 [D-amino-acid oxidase(Enzyme) wildtype]

H2O + O2 + D_Leucine -> NH3 + 4_Methyl_2_oxopentanoate + H2O2		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium pyrophosphate, 5 µg/ml FAD, 50 µg/ml BSA, 180 µg/ml Peroxidase, 750 µg/ml sodium 3, 5-dichloro-2-hydroxybenzenesulphonic acid, 112.5 µg/ml 4-aminoantipyrine, 7.5 mM sodium azide
PH: 8
Temperature: 37
uniprot:Q6TGN2 [D-amino-acid oxidase(Enzyme) wildtype]

H2O + O2 + D_Lysine -> H2O2 + 6_Amino_2_oxohexanoate + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium pyrophosphate, 5 µg/ml FAD, 50 µg/ml BSA, 180 µg/ml Peroxidase, 750 µg/ml sodium 3, 5-dichloro-2-hydroxybenzenesulphonic acid, 112.5 µg/ml 4-aminoantipyrine, 7.5 mM sodium azide
PH: 8
Temperature: 37
uniprot:Q6TGN2 [D-amino-acid oxidase(Enzyme) wildtype]

O2 + D_Serine + H2O -> 3_Hydroxypyruvate + NH3 + H2O2		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium pyrophosphate, 5 µg/ml FAD, 50 µg/ml BSA, 180 µg/ml Peroxidase, 750 µg/ml sodium 3, 5-dichloro-2-hydroxybenzenesulphonic acid, 112.5 µg/ml 4-aminoantipyrine, 7.5 mM sodium azide
PH: 8
Temperature: 37
uniprot:P80324 [D-amino-acid oxidase(Enzyme) mutant Y238S]

D_Alanine + O2 + H2O -> Pyruvate + NH3 + H2O2		 (et / (( (( (( (1 / kcat) ) + (phiA / A)) ) + (phiB / B)) ) + (phiAB / (A * B)))) buffer: 50 mM sodium pyrophosphate, 1% glycerol, 0.3 mM EDTA, 0.5 mM 2-mercaptoethanol.
PH: 8.5
Temperature: 25
uniprot:P80324 [D-amino-acid oxidase(Enzyme) mutant Y238F]

D_Alanine + O2 + H2O -> Pyruvate + H2O2 + NH3		 (et / (( (( (( (1 / kcat) ) + (phiA / A)) ) + (phiB / B)) ) + (phiAB / (A * B)))) buffer: 50 mM sodium pyrophosphate, 1% glycerol, 0.3 mM EDTA, 0.5 mM 2-mercaptoethanol.
PH: 8.5
Temperature: 25
uniprot:P80324 [D-amino-acid oxidase(Enzyme) wildtype]

H2O + O2 + D_Alanine -> Pyruvate + NH3 + H2O2		 (et / (( (( (( (1 / kcat) ) + (phiA / A)) ) + (phiB / B)) ) + (phiAB / (A * B)))) buffer: 50 mM sodium pyrophosphate, 1% glycerol, 0.3 mM EDTA, 0.5 mM 2-mercaptoethanol, 200 mM Glucose, 0.1 µM glucose oxidase, 0.03 µM catalase
PH: 8.5
Temperature: 36
uniprot:Q99042 [D-amino-acid oxidase(Enzyme) wildtype]

D_Alanine + O2 + H2O -> Pyruvate + NH3 + H2O2		 (et / (( (( (( (1 / kcat) ) + (phiA / A)) ) + (phiB / B)) ) + (phiAB / (A * B)))) buffer: 50 mM sodium pyrophosphate, 1% glycerol, 0.3 mM EDTA, 0.5 mM 2-mercaptoethanol, 200 mM Glucose, 0.1 µM glucose oxidase, 0.03 µM catalase
PH: 8.5
Temperature: 36
uniprot:P80324 [D-amino-acid oxidase(Enzyme) wildtype]

H2O + O2 + D_Valine -> NH3 + H2O2 + alpha_Ketovaline		 (et / (( (( (( (1 / kcat) ) + (phiA / A)) ) + (phiB / B)) ) + (phiAB / (A * B)))) buffer: 50 mM sodium pyrophosphate, 1% glycerol, 0.3 mM EDTA, 0.5 mM 2-mercaptoethanol, 200 mM Glucose, 0.1 µM glucose oxidase, 0.03 µM catalase
PH: 8.5
Temperature: 36
uniprot:Q99042 [D-amino-acid oxidase(Enzyme) wildtype]

D_Valine + H2O + O2 -> H2O2 + alpha_Ketovaline + NH3		 (et / (( (( (( (1 / kcat) ) + (phiA / A)) ) + (phiB / B)) ) + (phiAB / (A * B)))) buffer: 50 mM sodium pyrophosphate, 1% glycerol, 0.3 mM EDTA, 0.5 mM 2-mercaptoethanol, 200 mM Glucose, 0.1 µM glucose oxidase, 0.03 µM catalase
PH: 8.5
Temperature: 36
uniprot:Q95XG9 [D-amino-acid oxidase(Enzyme) wildtype Y69Ap]

O2 + H2O + D_Alanine -> H2O2 + Pyruvate + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM sodium pyrophosphate, 50 µM FAD
PH: 8.3
Temperature: 37
uniprot:Q95XG9 [D-amino-acid oxidase(Enzyme) wildtype Y69Ap]

H2O + O2 + D_Arginine -> 5_Guanidino_2_oxopentanoate + NH3 + H2O2		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM sodium pyrophosphate, 50 µM FAD
PH: 8.3
Temperature: 37
uniprot:Q95XG9 [D-amino-acid oxidase(Enzyme) wildtype Y69Ap]

O2 + D_Methionine + H2O -> 4_Methylthio_2_oxobutanoate + NH3 + H2O2		 (( (Vmax * A) ) / (Km + A)) buffer: 40 mM sodium pyrophosphate, 50 µM FAD
PH: 8.3
Temperature: 37

Pathways

pathway id name
Reactome:R-HSA-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-RNO-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-SSC-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-DDI-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-SPO-71291 Amino acid and derivative metabolism
Reactome:R-SSC-1430728 Metabolism
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-SSC-71291 Amino acid and derivative metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP5166 Glyoxylate metabolism
PathBank:SMP0120710 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0120722 Hypoacetylaspartia
PathBank:SMP0120758 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0120790 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0120569 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0000020 Arginine and Proline Metabolism
PathBank:SMP0000362 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0000188 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0000504 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0087426 Aspartate Metabolism
PathBank:SMP0063596 Arginine and Proline Metabolism
PathBank:SMP0063608 D-Arginine and D-Ornithine Metabolism
PathBank:SMP0120681 Canavan Disease
PathBank:SMP0120721 Hyperprolinemia Type II
PathBank:SMP0120761 Prolidase Deficiency (PD)
PathBank:SMP0120789 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0120499 Hyperprolinemia Type I
PathBank:SMP0120539 Prolinemia Type II
PathBank:SMP0120572 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0000207 Prolidase Deficiency (PD)
PathBank:SMP0000208 Prolinemia Type II
PathBank:SMP0000507 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0087433 D-Arginine and D-Ornithine Metabolism
PathBank:SMP0120788 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0120501 Hyperprolinemia Type II
PathBank:SMP0120541 Prolidase Deficiency (PD)
PathBank:SMP0120570 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0000360 Hyperprolinemia Type II
PathBank:SMP0000363 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0000505 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0087276 Aspartate Metabolism
PathBank:SMP0120675 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0120719 Hyperprolinemia Type I
PathBank:SMP0120759 Prolinemia Type II
PathBank:SMP0120791 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0120454 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0120490 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0120538 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0120571 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0000036 D-Arginine and D-Ornithine Metabolism
PathBank:SMP0000361 Hyperprolinemia Type I
PathBank:SMP0000506 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0087283 Arginine and Proline Metabolism
PathBank:SMP0087291 D-Arginine and D-Ornithine Metabolism