EC: 1.4.1.3

glutamate dehydrogenase [NAD(P)+] (L-glutamate:NAD(P)+ oxidoreductase (deaminating))

enzyme lambda metabolic reaction experiment
uniprot:P10860 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NAD+ + H2O + L_Glutamate -> H+ + NH3 + NADH + 2_Oxoglutarate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 150 mM KCl, 2 mM EGTA, 0.01 mM Rotenone
PH: 7.6
Temperature: 37
uniprot:O52310 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NADP+ + L_Glutamate + H2O -> NH3 + NADPH + H+ + 2_Oxoglutarate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Potassium phosphate, 1 mM EDTA
PH: 7.6
Temperature: 90
uniprot:O52310 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NADP+ + L_Glutamate + H2O -> NADPH + NH3 + 2_Oxoglutarate + H+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Potassium phosphate, 1 mM EDTA
PH: 7.6
Temperature: 90
uniprot:O52310 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

2_Oxoglutarate + NH3 + H+ + NADH -> H2O + NAD+ + L_Glutamate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Potassium phosphate, 1 mM EDTA
PH: 7.6
Temperature: 90
uniprot:O52310 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NADPH + 2_Oxoglutarate + H+ + NH3 -> H2O + NADP+ + L_Glutamate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Potassium phosphate, 1 mM EDTA, 50 mM Ammonium chloride
PH: 7.6
Temperature: 90
uniprot:O52310 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NH3 + 2_Oxoglutarate + H+ + NADPH -> L_Glutamate + NADP+ + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Potassium phosphate, 1 mM EDTA
PH: 7.6
Temperature: 90
uniprot:O52310 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

H+ + NADPH + NH3 + 2_Oxoglutarate -> H2O + NADP+ + L_Glutamate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Potassium phosphate, 1 mM EDTA
PH: 7.6
Temperature: 90
uniprot:P80319 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NH3 + 2_Oxoglutarate + H+ + NADH -> NAD+ + L_Glutamate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole
PH: 7.15
Temperature: 85
uniprot:P80319 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NH3 + H+ + NADH + 2_Oxoglutarate -> L_Glutamate + H2O + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole
PH: 7.15
Temperature: 85
uniprot:P80319 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

H2O + NAD+ + L_Glutamate -> H+ + NH3 + 2_Oxoglutarate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole
PH: 7.15
Temperature: 85
uniprot:P80319 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NH3 + 2_Oxoglutarate + H+ + NADPH -> NADP+ + L_Glutamate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole
PH: 7.15
Temperature: 85
uniprot:P80319 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

H+ + NADPH + 2_Oxoglutarate + NH3 -> NADP+ + H2O + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole
PH: 7.15
Temperature: 85
uniprot:P80319 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NADPH + 2_Oxoglutarate + H+ + NH3 -> L_Glutamate + NADP+ + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole
PH: 7.15
Temperature: 85
uniprot:P80319 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NAD+ + L_Glutamate + H2O -> NH3 + 2_Oxoglutarate + H+ + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole
PH: 7.15
Temperature: 85
uniprot:P00367 [ADP]

H2O + NAD+ + L_Glutamate -> H+ + NH3 + NADH + 2_Oxoglutarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2.6 mM EDTA
PH: 9.5
Temperature: 25
uniprot:P00367 [ADP]

NAD+ + H2O + L_Glutamate -> NADH + H+ + 2_Oxoglutarate + NH3		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2.6 mM EDTA
PH: 9.5
Temperature: 25
uniprot:P00367 [ADP]

H2O + L_Glutamate + NAD+ -> NH3 + 2_Oxoglutarate + H+ + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2.6 mM EDTA
PH: 9.5
Temperature: 25
uniprot:P00367 [ADP]

L_Glutamate + H2O + NAD+ -> H+ + NH3 + 2_Oxoglutarate + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2.6 mM EDTA
PH: 9.5
Temperature: 25
uniprot:P00367 [ADP]

NAD+ + H2O + L_Glutamate -> 2_Oxoglutarate + NH3 + NADH + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2.6 mM EDTA
PH: 9.5
Temperature: 25
uniprot:P00367 [ADP]

H2O + L_Glutamate + NAD+ -> NADH + NH3 + H+ + 2_Oxoglutarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2.6 mM EDTA
PH: 9.5
Temperature: 25
uniprot:P00367 [ADP]

H2O + L_Glutamate + NAD+ -> H+ + NADH + NH3 + 2_Oxoglutarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2.6 mM EDTA
PH: 9.5
Temperature: 25
uniprot:P00367 [ADP]

NAD+ + H2O + L_Glutamate -> H+ + NADH + NH3 + 2_Oxoglutarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2.6 mM EDTA
PH: 9.5
Temperature: 25
uniprot:O59650 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NADPH + H+ + NH3 + 2_Oxoglutarate -> L_Glutamate + H2O + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Sodium phosphate
PH: 7.6
Temperature: 70
uniprot:O59650 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NADPH + 2_Oxoglutarate + H+ + NH3 -> NADP+ + H2O + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Sodium phosphate
PH: 7.6
Temperature: 70
uniprot:O59650 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NADPH + H+ + 2_Oxoglutarate + NH3 -> NADP+ + H2O + L_Glutamate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Sodium phosphate
PH: 7.6
Temperature: 70
uniprot:O59650 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

L_Glutamate + NADP+ + H2O -> 2_Oxoglutarate + NH3 + NADPH + H+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Sodium phosphate
PH: 7.6
Temperature: 80
uniprot:O59650 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

L_Glutamate + H2O + NADP+ -> NH3 + NADPH + 2_Oxoglutarate + H+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Sodium phosphate
PH: 7.6
Temperature: 80
uniprot:P49448 [ADP]

2_Oxoglutarate + H+ + NH3 + NADH -> H2O + NAD+ + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
uniprot:P00367 [ADP]

2_Oxoglutarate + NADH + H+ + NH3 -> H2O + L_Glutamate + NAD+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
uniprot:P49448 [ADP]

2_Oxoglutarate + H+ + NADH + NH3 -> H2O + NAD+ + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
uniprot:P49448 [ADP]

NH3 + H+ + NADH + 2_Oxoglutarate -> NAD+ + L_Glutamate + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
uniprot:P49448 [ADP]

2_Oxoglutarate + NH3 + NADH + H+ -> NAD+ + L_Glutamate + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
uniprot:P00367 [ADP]

H+ + NADH + NH3 + 2_Oxoglutarate -> NAD+ + H2O + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
uniprot:O59650 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

2_Oxoglutarate + NH3 + NADH + H+ -> NAD+ + L_Glutamate + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Sodium phosphate
PH: 7.6
Temperature: 70
uniprot:O59650 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

H+ + NH3 + 2_Oxoglutarate + NADPH -> L_Glutamate + H2O + NADP+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Sodium phosphate
PH: 7.6
Temperature: 70
uniprot:O59650 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

L_Glutamate + NADP+ + H2O -> NADPH + NH3 + 2_Oxoglutarate + H+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Sodium phosphate
PH: 7.6
Temperature: 80
uniprot:P80053 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

2_Oxoglutarate + NADH + H+ + NH3 -> H2O + NAD+ + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine-HCl, 2.5 mM EDTA
PH: 7.6
Temperature: 70
uniprot:P80319 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

L_Glutamate + H2O + NADP+ -> 2_Oxoglutarate + NH3 + H+ + NADPH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole
PH: 7.15
Temperature: 85
uniprot:P80319 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

L_Glutamate + H2O + NADP+ -> H+ + NH3 + 2_Oxoglutarate + NADPH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole
PH: 7.15
Temperature: 85
uniprot:P00367 [ADP]

H2O + NAD+ + L_Glutamate -> 2_Oxoglutarate + NH3 + NADH + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2.6 mM EDTA
PH: 9.5
Temperature: 25
uniprot:P00367 [ADP]

NAD+ + L_Glutamate + H2O -> NADH + 2_Oxoglutarate + NH3 + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2.6 mM EDTA
PH: 9.5
Temperature: 25
uniprot:P00367 [ADP]

NAD+ + L_Glutamate + H2O -> 2_Oxoglutarate + NADH + H+ + NH3		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2.6 mM EDTA
PH: 9.5
Temperature: 25
uniprot:P00367 [ADP]

H2O + NAD+ + L_Glutamate -> NH3 + H+ + 2_Oxoglutarate + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2.6 mM EDTA
PH: 9.5
Temperature: 25
- [ADP]

NADH + H+ + NH3 + 2_Oxoglutarate -> H2O + NAD+ + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

H+ + 2_Oxoglutarate + NH3 + NADH -> L_Glutamate + H2O + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

2_Oxoglutarate + NH3 + NADH + H+ -> H2O + L_Glutamate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
uniprot:P80053 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NADH + H+ + 2_Oxoglutarate + NH3 -> L_Glutamate + H2O + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine-HCl, 2.5 mM EDTA
PH: 7.6
Temperature: 70
uniprot:P80053 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

2_Oxoglutarate + NH3 + NADPH + H+ -> L_Glutamate + NADP+ + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine-HCl, 2.5 mM EDTA
PH: 7.6
Temperature: 70
uniprot:P80053 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NAD+ + L_Glutamate + H2O -> NH3 + 2_Oxoglutarate + NADH + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine-HCl, 2.5 mM EDTA
PH: 7.6
Temperature: 70
uniprot:P80053 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NAD+ + L_Glutamate + H2O -> 2_Oxoglutarate + NH3 + NADH + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine-HCl, 2.5 mM EDTA
PH: 7.6
Temperature: 70
uniprot:P80053 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

H+ + NH3 + NADPH + 2_Oxoglutarate -> H2O + L_Glutamate + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine-HCl, 2.5 mM EDTA
PH: 7.6
Temperature: 70
uniprot:P80053 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NADP+ + H2O + L_Glutamate -> 2_Oxoglutarate + NADPH + H+ + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine-HCl, 2.5 mM EDTA
PH: 7.6
Temperature: 70
uniprot:P80053 [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NADP+ + L_Glutamate + H2O -> NH3 + H+ + 2_Oxoglutarate + NADPH		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine-HCl, 2.5 mM EDTA
PH: 7.6
Temperature: 70
- [ADP]

2_Oxoglutarate + NADH + H+ + NH3 -> H2O + L_Glutamate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

H+ + NADH + 2_Oxoglutarate + NH3 -> H2O + NAD+ + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

NADH + H+ + NH3 + 2_Oxoglutarate -> L_Glutamate + NAD+ + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

2_Oxoglutarate + NADH + H+ + NH3 -> L_Glutamate + NAD+ + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

H+ + 2_Oxoglutarate + NADH + NH3 -> NAD+ + L_Glutamate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

NH3 + H+ + 2_Oxoglutarate + NADH -> NAD+ + H2O + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

2_Oxoglutarate + NADH + NH3 + H+ -> H2O + NAD+ + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

2_Oxoglutarate + H+ + NADH + NH3 -> L_Glutamate + NAD+ + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

NADH + 2_Oxoglutarate + H+ + NH3 -> H2O + L_Glutamate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

NH3 + NADH + 2_Oxoglutarate + H+ -> NAD+ + H2O + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

NH3 + NADH + 2_Oxoglutarate + H+ -> L_Glutamate + H2O + NAD+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

NADH + NH3 + H+ + 2_Oxoglutarate -> H2O + NAD+ + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

NH3 + NADH + 2_Oxoglutarate + H+ -> NAD+ + H2O + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

NADH + 2_Oxoglutarate + H+ + NH3 -> NAD+ + L_Glutamate + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

2_Oxoglutarate + NH3 + NADH + H+ -> H2O + NAD+ + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

NADH + 2_Oxoglutarate + H+ + NH3 -> H2O + NAD+ + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

2_Oxoglutarate + NH3 + H+ + NADH -> H2O + L_Glutamate + NAD+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

H+ + 2_Oxoglutarate + NADH + NH3 -> NAD+ + H2O + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

NH3 + NADH + H+ + 2_Oxoglutarate -> L_Glutamate + NAD+ + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

H+ + NADH + NH3 + 2_Oxoglutarate -> H2O + L_Glutamate + NAD+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

NADH + NH3 + 2_Oxoglutarate + H+ -> NAD+ + L_Glutamate + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

NADH + H+ + 2_Oxoglutarate + NH3 -> H2O + L_Glutamate + NAD+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

H+ + 2_Oxoglutarate + NADH + NH3 -> NAD+ + L_Glutamate + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

2_Oxoglutarate + H+ + NADH + NH3 -> H2O + L_Glutamate + NAD+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [ADP]

NADH + NH3 + 2_Oxoglutarate + H+ -> H2O + NAD+ + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine, 2.6 mM EDTA
PH: 8
Temperature: 25
- [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NH3 + NADH + H+ + 2_Oxoglutarate -> NAD+ + H2O + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM TEA-HCl, 2.5 mM EDTA, 1.0 mM ADP
PH: 7.6
Temperature: 37
- [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NH3 + H+ + NADH + 2_Oxoglutarate -> H2O + L_Glutamate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM TEA-HCl, 2.5 mM EDTA, 1.0 mM ADP
PH: 7.6
Temperature: 37
- [glutamate dehydrogenase [NAD(P)+](Enzyme) wildtype]

NH3 + NADH + H+ + 2_Oxoglutarate -> L_Glutamate + H2O + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM TEA-HCl, 2.5 mM EDTA, 1.0 mM ADP
PH: 7.6
Temperature: 37
uniprot:P10860 [Mg2+]

NADPH + NH3 + 2_Oxoglutarate + H+ -> L_Glutamate + H2O + NADP+		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 1.2 mM ADP
PH: 7.2
Temperature: 37
uniprot:P10860 [Mg2+]

H+ + 2_Oxoglutarate + NADPH + NH3 -> L_Glutamate + NADP+ + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 1.2 mM ADP
PH: 7.2
Temperature: 37
uniprot:P10860 [Mg2+]

NH3 + 2_Oxoglutarate + NADPH + H+ -> NADP+ + L_Glutamate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 1.2 mM ADP
PH: 7.2
Temperature: 37
uniprot:P10860 [Mg2+]

2_Oxoglutarate + NH3 + NADPH + H+ -> NADP+ + L_Glutamate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 1.2 mM ADP
PH: 7.2
Temperature: 37
uniprot:P10860 [Mg2+]

H2O + NADP+ + L_Glutamate -> 2_Oxoglutarate + NADPH + H+ + NH3		 (( (Vmax * A) ) / (Km_A + A)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 1.2 mM ADP
PH: 7.2
Temperature: 37
uniprot:P10860 [Mg2+]

NH3 + NADPH + H+ + 2_Oxoglutarate -> NADP+ + H2O + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 1.2 mM ADP
PH: 7.2
Temperature: 37
uniprot:P10860 [Mg2+]

L_Glutamate + H2O + NADP+ -> H+ + NH3 + NADPH + 2_Oxoglutarate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 1.2 mM ADP
PH: 7.2
Temperature: 37
uniprot:P10860 [Mg2+]

L_Glutamate + H2O + NADP+ -> 2_Oxoglutarate + NH3 + H+ + NADPH		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 1.2 mM ADP
PH: 7.2
Temperature: 37
uniprot:P10860 [Mg2+]

NADPH + 2_Oxoglutarate + H+ + NH3 -> L_Glutamate + H2O + NADP+		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 1.2 mM ADP
PH: 7.2
Temperature: 37
uniprot:P10860 [Mg2+]

L_Glutamate + H2O + NADP+ -> 2_Oxoglutarate + H+ + NH3 + NADPH		 (( (Vmax * B) ) / (Km_B + B)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 1.2 mM ADP
PH: 7.2
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-5505 L-glutamate and L-glutamine biosynthesis
BioCyc:META_PWY-5766 L-glutamate degradation X
BioCyc:META_GLUTAMATE-DEG1-PWY L-glutamate degradation I
BioCyc:ARA_ALACAT2-PWY L-alanine degradation II (to D-lactate)
BioCyc:ARA_GLUTAMATE-DEG1-PWY L-glutamate degradation I
BioCyc:ARA_ARGININE-SYN4-PWY L-ornithine biosynthesis II
BioCyc:HUMAN_GLUDEG-I-PWY GABA shunt
BioCyc:META_GLUDEG-I-PWY GABA shunt
BioCyc:ARA_GLUTAMATE-SYN2-PWY L-glutamate biosynthesis II
BioCyc:MOUSE_GLUTAMINE-DEG1-PWY glutamine degradation I
BioCyc:HUMAN_ARGININE-SYN4-PWY ornithine de novo biosynthesis
BioCyc:META_GLUTAMATE-SYN2-PWY L-glutamate biosynthesis II
BioCyc:MOUSE_GLUTAMATE-SYN2-PWY glutamate biosynthesis II
BioCyc:MOUSE_PROLINE-SYN2-PWY proline biosynthesis II
BioCyc:MOUSE_ARGININE-SYN4-PWY arginine biosynthesis IV
BioCyc:BTHE_GLUTSYNIII-PWY glutamate biosynthesis III
BioCyc:HUMAN_GLUTAMATE-SYN2-PWY glutamate biosynthesis/degradation
BioCyc:META_ARGININE-SYN4-PWY L-ornithine biosynthesis II
BioCyc:MOUSE_GLUTAMINE-SYN2-PWY glutamine biosynthesis II
BioCyc:MOUSE_PWY-5766 glutamate degradation X
PlantCyc:ARA_GLUTAMATE-DEG1-PWY L-glutamate degradation I
PlantCyc:ARA_ARGININE-SYN4-PWY L-ornithine biosynthesis II
PlantCyc:PLANT_ARGININE-SYN4-PWY L-ornithine biosynthesis II
PlantCyc:ARA_ALACAT2-PWY L-alanine degradation II (to D-lactate)
PlantCyc:ARA_GLUTAMATE-SYN2-PWY L-glutamate biosynthesis II
PlantCyc:PLANT_GLUTAMATE-DEG1-PWY L-glutamate degradation I
Reactome:R-RNO-8964539 Glutamate and glutamine metabolism
Reactome:R-DDI-8964539 Glutamate and glutamine metabolism
Reactome:R-MMU-8964539 Glutamate and glutamine metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-RNO-1592230 Mitochondrial biogenesis
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-MMU-2151201 Transcriptional activation of mitochondrial biogenesis
Reactome:R-RNO-1852241 Organelle biogenesis and maintenance
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-MMU-1852241 Organelle biogenesis and maintenance
Reactome:R-RNO-2151201 Transcriptional activation of mitochondrial biogenesis
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-MMU-1592230 Mitochondrial biogenesis
WikiPathways:WP4595 Urea cycle and associated pathways
WikiPathways:WP497 Urea cycle and metabolism of amino groups
WikiPathways:WP5220 Metabolic reprogramming in pancreatic cancer
WikiPathways:WP5312 Metabolic pathways of fibroblasts
PathBank:SMP0120666 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120682 Carbamoyl Phosphate Synthetase Deficiency
PathBank:SMP0120686 Citrullinemia Type I
PathBank:SMP0120710 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0120714 Homocarnosinosis
PathBank:SMP0120758 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0120762 Ornithine Transcarbamylase Deficiency (OTC Deficiency)
PathBank:SMP0120790 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0120850 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120456 Argininosuccinic Aciduria
PathBank:SMP0120496 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0120569 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0000059 Urea Cycle
PathBank:SMP0000020 Arginine and Proline Metabolism
PathBank:SMP0000009 Ammonia Recycling
PathBank:SMP0000339 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0000362 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0000188 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0000001 Citrullinemia Type I
PathBank:SMP0000504 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0002358 The Oncogenic Action of L-2-Hydroxyglutarate in Hydroxyglutaric aciduria
PathBank:SMP0087278 Glutamate Metabolism
PathBank:SMP0087282 Ammonia Recycling
PathBank:SMP0063579 Ammonia Recycling
PathBank:SMP0063596 Arginine and Proline Metabolism
PathBank:SMP0063621 Glucose-Alanine Cycle
PathBank:SMP0120677 Argininosuccinic Aciduria
PathBank:SMP0120721 Hyperprolinemia Type II
PathBank:SMP0120761 Prolidase Deficiency (PD)
PathBank:SMP0120789 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0120455 Argininemia
PathBank:SMP0120499 Hyperprolinemia Type I
PathBank:SMP0120539 Prolinemia Type II
PathBank:SMP0120572 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0000127 Glucose-Alanine Cycle
PathBank:SMP0000385 Homocarnosinosis
PathBank:SMP0000207 Prolidase Deficiency (PD)
PathBank:SMP0000208 Prolinemia Type II
PathBank:SMP0000003 Argininosuccinic Aciduria
PathBank:SMP0000136 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0000507 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0000567 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120676 Argininemia
PathBank:SMP0120716 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0120788 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0120445 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120501 Hyperprolinemia Type II
PathBank:SMP0120541 Prolidase Deficiency (PD)
PathBank:SMP0120570 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0000360 Hyperprolinemia Type II
PathBank:SMP0000363 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0000205 Ornithine Transcarbamylase Deficiency (OTC Deficiency)
PathBank:SMP0000505 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0000654 Warburg Effect
PathBank:SMP0002359 The Oncogenic Action of D-2-Hydroxyglutarate in Hydroxyglutaric aciduria
PathBank:SMP0087316 Glucose-Alanine Cycle
PathBank:SMP0063590 Glutamate Metabolism
PathBank:SMP0120659 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120675 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0120719 Hyperprolinemia Type I
PathBank:SMP0120759 Prolinemia Type II
PathBank:SMP0120791 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0120438 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120454 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0120462 Carbamoyl Phosphate Synthetase Deficiency
PathBank:SMP0120466 Citrullinemia Type I
PathBank:SMP0120490 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0120494 Homocarnosinosis
PathBank:SMP0120538 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0120542 Ornithine Transcarbamylase Deficiency (OTC Deficiency)
PathBank:SMP0120571 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0120631 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000072 Glutamate Metabolism
PathBank:SMP0000243 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000361 Hyperprolinemia Type I
PathBank:SMP0000357 Argininemia
PathBank:SMP0000002 Carbamoyl Phosphate Synthetase Deficiency
PathBank:SMP0000506 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0002298 Glutaminolysis and Cancer
PathBank:SMP0002415 Alanine Metabolism
PathBank:SMP0002427 Glutamic Acid Metabolism
PathBank:SMP0086930 Warburg Effect
PathBank:SMP0087283 Arginine and Proline Metabolism
PathBank:SMP0087319 Urea Cycle
PathBank:SMP0087363 Warburg Effect
PathBank:SMP0063686 Urea Cycle