EC: 1.3.8.7

medium-chain acyl-CoA dehydrogenase (medium-chain acyl-CoA:electron-transfer flavoprotein 2,3-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P41367 [medium-chain-acyl-CoA dehydrogenase(Enzyme) wildtype]

FAD + Octanoyl_CoA -> trans_Oct_2_enoyl_CoA + FADH2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, ferricenium in excess
PH: 7.6
Temperature: 25
uniprot:P41367 [medium-chain-acyl-CoA dehydrogenase(Enzyme) wildtype]

FAD + Octanoyl_CoA -> FADH2 + trans_Oct_2_enoyl_CoA		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, ferricenium in excess
PH: 7.6
Temperature: 25
uniprot:P41367 [medium-chain-acyl-CoA dehydrogenase(Enzyme) wildtype]

Octanoyl_CoA + FAD -> FADH2 + trans_Oct_2_enoyl_CoA		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, ferricenium in excess
PH: 7.6
Temperature: 25
uniprot:P41367 [medium-chain-acyl-CoA dehydrogenase(Enzyme) wildtype]

Octanoyl_CoA + FAD -> FADH2 + trans_Oct_2_enoyl_CoA		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, ferricenium in excess
PH: 7.6
Temperature: 25
uniprot:P11310 [medium-chain-acyl-CoA dehydrogenase(Enzyme) wildtype]

FAD + Octanoyl_CoA -> trans_Oct_2_enoyl_CoA + FADH2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, ferricenium in excess
PH: 7.6
Temperature: 25
uniprot:Q92947 [medium-chain-acyl-CoA dehydrogenase(Enzyme) wildtype]

Acceptor + Hexanoyl_CoA -> Reduced_acceptor + trans_Hex_2_enoyl_CoA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Tris-HCl
PH: 8
Temperature: 25
uniprot:Q92947 [medium-chain-acyl-CoA dehydrogenase(Enzyme) wildtype]

Acceptor + Pentanoyl_CoA -> trans_Pent_2_enoyl_CoA + Reduced_acceptor		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Tris-HCl
PH: 8
Temperature: 25
uniprot:Q92947 [medium-chain-acyl-CoA dehydrogenase(Enzyme) wildtype]

5_Hexenoyl_CoA + Acceptor -> Reduced_acceptor + Hexa_2,5_dienoyl_CoA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Tris-HCl
PH: 8
Temperature: 25
uniprot:Q92947 [medium-chain-acyl-CoA dehydrogenase(Enzyme) wildtype]

Methylglutaryl_CoA + Acceptor -> Reduced_acceptor + 2,3_Dehydromethylglutaryl_CoA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Tris-HCl
PH: 8
Temperature: 25
uniprot:Q92947 [medium-chain-acyl-CoA dehydrogenase(Enzyme) wildtype]

Glutaramyl_CoA + Acceptor -> Reduced_acceptor + 5_Amino_5_oxopent_2_enoate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Tris-HCl
PH: 8
Temperature: 25
uniprot:Q92947 [medium-chain-acyl-CoA dehydrogenase(Enzyme) wildtype]

4_Nitrobutyryl_CoA + Acceptor -> Reduced_acceptor + 4_Nitronate_but_2_enoyl_CoA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Tris-HCl
PH: 8
Temperature: 25

Pathways

pathway id name
BioCyc:META_FAO-PWY fatty acid β-oxidation I
BioCyc:ECO_FAO-PWY fatty acid β-oxidation I
BioCyc:ECOL316407_FAO-PWY fatty acid β-oxidation I
BioCyc:MTBH37RV_FAO-PWY fatty acid β-oxidation I
BioCyc:MOUSE_FAO-PWY fatty acid β-oxidation I
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-BTA-77286 mitochondrial fatty acid beta-oxidation of saturated fatty acids
Reactome:R-BTA-77346 Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA
Reactome:R-BTA-77288 mitochondrial fatty acid beta-oxidation of unsaturated fatty acids
Reactome:R-DME-77348 Beta oxidation of octanoyl-CoA to hexanoyl-CoA
Reactome:R-HSA-400206 Regulation of lipid metabolism by PPARalpha
Reactome:R-HSA-77286 mitochondrial fatty acid beta-oxidation of saturated fatty acids
Reactome:R-HSA-77346 Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA
Reactome:R-HSA-77288 mitochondrial fatty acid beta-oxidation of unsaturated fatty acids
Reactome:R-HSA-611105 Respiratory electron transport
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-77286 mitochondrial fatty acid beta-oxidation of saturated fatty acids
Reactome:R-MMU-77346 Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA
Reactome:R-MMU-77288 mitochondrial fatty acid beta-oxidation of unsaturated fatty acids
Reactome:R-MMU-611105 Respiratory electron transport
Reactome:R-RNO-8978868 Fatty acid metabolism
Reactome:R-RNO-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-RNO-77310 Beta oxidation of lauroyl-CoA to decanoyl-CoA-CoA
Reactome:R-RNO-611105 Respiratory electron transport
Reactome:R-SSC-77285 Beta oxidation of myristoyl-CoA to lauroyl-CoA
Reactome:R-BTA-8978868 Fatty acid metabolism
Reactome:R-BTA-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-77286 mitochondrial fatty acid beta-oxidation of saturated fatty acids
Reactome:R-DME-77346 Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA
Reactome:R-DME-77288 mitochondrial fatty acid beta-oxidation of unsaturated fatty acids
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-HSA-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-HSA-77310 Beta oxidation of lauroyl-CoA to decanoyl-CoA-CoA
Reactome:R-MMU-8978868 Fatty acid metabolism
Reactome:R-MMU-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-MMU-77310 Beta oxidation of lauroyl-CoA to decanoyl-CoA-CoA
Reactome:R-RNO-77285 Beta oxidation of myristoyl-CoA to lauroyl-CoA
Reactome:R-SSC-1430728 Metabolism
Reactome:R-SSC-556833 Metabolism of lipids
Reactome:R-BTA-77348 Beta oxidation of octanoyl-CoA to hexanoyl-CoA
Reactome:R-DME-8978868 Fatty acid metabolism
Reactome:R-HSA-1989781 PPARA activates gene expression
Reactome:R-HSA-77348 Beta oxidation of octanoyl-CoA to hexanoyl-CoA
Reactome:R-HSA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-HSA-6799198 Complex I biogenesis
Reactome:R-MMU-77348 Beta oxidation of octanoyl-CoA to hexanoyl-CoA
Reactome:R-MMU-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-MMU-6799198 Complex I biogenesis
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-77286 mitochondrial fatty acid beta-oxidation of saturated fatty acids
Reactome:R-RNO-77346 Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA
Reactome:R-RNO-77288 mitochondrial fatty acid beta-oxidation of unsaturated fatty acids
Reactome:R-RNO-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-RNO-6799198 Complex I biogenesis
Reactome:R-SSC-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-SSC-77310 Beta oxidation of lauroyl-CoA to decanoyl-CoA-CoA
Reactome:R-DME-556833 Metabolism of lipids
Reactome:R-DME-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-77285 Beta oxidation of myristoyl-CoA to lauroyl-CoA
Reactome:R-MMU-77285 Beta oxidation of myristoyl-CoA to lauroyl-CoA
Reactome:R-RNO-77348 Beta oxidation of octanoyl-CoA to hexanoyl-CoA
Reactome:R-SSC-8978868 Fatty acid metabolism
Reactome:R-SSC-77286 mitochondrial fatty acid beta-oxidation of saturated fatty acids
WikiPathways:WP5123 Mitochondrial fatty acid oxidation disorders
WikiPathways:WP4324 Mitochondrial complex I assembly model OXPHOS system
WikiPathways:WP2318 Fatty acid oxidation
WikiPathways:WP5318 Female steroid hormones in cardiomyocyte energy metabolism
PathBank:SMP0120662 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120734 Malonic Aciduria
PathBank:SMP0120786 Malonyl-CoA Decarboxylase Deficiency
PathBank:SMP0120806 3-Hydroxyisobutyric Aciduria
PathBank:SMP0120822 Carnitine Palmitoyl Transferase Deficiency I
PathBank:SMP0120826 Medium Chain Acyl-CoA Dehydrogenase Deficiency (MCAD)
PathBank:SMP0120440 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120444 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120476 Ethylmalonic Encephalopathy
PathBank:SMP0120516 Maple Syrup Urine Disease
PathBank:SMP0120520 Short-Chain Acyl-CoA Dehydrogenase Deficiency (SCAD Deficiency)
PathBank:SMP0120528 Methylmalonic Aciduria Due to Cobalamin-Related Disorders
PathBank:SMP0120589 Isovaleric Acidemia
PathBank:SMP0120605 Very-Long-Chain Acyl-CoA Dehydrogenase Deficiency (VLCAD)
PathBank:SMP0120609 Trifunctional Protein Deficiency
PathBank:SMP0000032 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0000051 Fatty Acid Metabolism
PathBank:SMP0000016 Propanoate Metabolism
PathBank:SMP0000173 beta-Ketothiolase Deficiency
PathBank:SMP0000199 Maple Syrup Urine Disease
PathBank:SMP0000384 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000235 Short-Chain Acyl-CoA Dehydrogenase Deficiency (SCAD Deficiency)
PathBank:SMP0000482 Mitochondrial Beta-Oxidation of Long Chain Saturated Fatty Acids
PathBank:SMP0000141 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0000524 Isovaleric Acidemia
PathBank:SMP0000540 Very-Long-Chain Acyl-CoA Dehydrogenase Deficiency (VLCAD)
PathBank:SMP0000568 Short-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (SCHAD)
PathBank:SMP0087358 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0087402 Propanoate Metabolism
PathBank:SMP0087418 Mitochondrial Beta-Oxidation of Long Chain Saturated Fatty Acids
PathBank:SMP0087430 Fatty Acid Metabolism
PathBank:SMP0063656 Propanoate Metabolism
PathBank:SMP0120661 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120665 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120745 Methylmalonic Aciduria
PathBank:SMP0120757 Propionic Acidemia
PathBank:SMP0120805 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0120825 Carnitine Palmitoyl Transferase Deficiency II
PathBank:SMP0120439 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120443 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120459 beta-Ketothiolase Deficiency
PathBank:SMP0120503 Isovaleric Aciduria
PathBank:SMP0120523 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120588 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120604 Long Chain Acyl-CoA Dehydrogenase Deficiency (LCAD)
PathBank:SMP0120632 Short-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (SCHAD)
PathBank:SMP0000138 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0000140 3-Methylglutaconic Aciduria Type III
PathBank:SMP0000238 Isovaleric Aciduria
PathBank:SMP0000185 Glutaric Aciduria Type I
PathBank:SMP0000481 Mitochondrial Beta-Oxidation of Medium Chain Saturated Fatty Acids
PathBank:SMP0000201 Methylmalonic Aciduria Due to Cobalamin-Related Disorders
PathBank:SMP0000523 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000539 Long Chain Acyl-CoA Dehydrogenase Deficiency (LCAD)
PathBank:SMP0087341 Propanoate Metabolism
PathBank:SMP0087373 Fatty Acid Metabolism
PathBank:SMP0087417 Mitochondrial Beta-Oxidation of Medium Chain Saturated Fatty Acids
PathBank:SMP0087521 Mitochondrial Beta-Oxidation of Medium Chain Saturated Fatty Acids
PathBank:SMP0063641 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0063689 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0120660 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120664 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120696 Ethylmalonic Encephalopathy
PathBank:SMP0120736 Maple Syrup Urine Disease
PathBank:SMP0120740 Short-Chain Acyl-CoA Dehydrogenase Deficiency (SCAD Deficiency)
PathBank:SMP0120748 Methylmalonic Aciduria Due to Cobalamin-Related Disorders
PathBank:SMP0120808 Isovaleric Acidemia
PathBank:SMP0120824 Very-Long-Chain Acyl-CoA Dehydrogenase Deficiency (VLCAD)
PathBank:SMP0120828 Trifunctional Protein Deficiency
PathBank:SMP0120441 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120525 Methylmalonic Aciduria
PathBank:SMP0120537 Propionic Acidemia
PathBank:SMP0120586 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0120606 Carnitine Palmitoyl Transferase Deficiency II
PathBank:SMP0000137 2-Methyl-3-hydroxybutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000237 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0000200 Methylmalonic Aciduria
PathBank:SMP0000521 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0000541 Carnitine Palmitoyl Transferase Deficiency II
PathBank:SMP0000545 Trifunctional Protein Deficiency
PathBank:SMP0087280 Fatty Acid Metabolism
PathBank:SMP0087360 Mitochondrial Beta-Oxidation of Long Chain Saturated Fatty Acids
PathBank:SMP0087416 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0087480 Propanoate Metabolism
PathBank:SMP0063640 Mitochondrial Beta-Oxidation of Medium Chain Saturated Fatty Acids
PathBank:SMP0120663 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120679 beta-Ketothiolase Deficiency
PathBank:SMP0120723 Isovaleric Aciduria
PathBank:SMP0120743 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120807 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120823 Long Chain Acyl-CoA Dehydrogenase Deficiency (LCAD)
PathBank:SMP0120851 Short-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (SCHAD)
PathBank:SMP0120442 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120514 Malonic Aciduria
PathBank:SMP0120567 Malonyl-CoA Decarboxylase Deficiency
PathBank:SMP0120587 3-Hydroxyisobutyric Aciduria
PathBank:SMP0120603 Carnitine Palmitoyl Transferase Deficiency I
PathBank:SMP0120607 Medium Chain Acyl-CoA Dehydrogenase Deficiency (MCAD)
PathBank:SMP0000236 Propionic Acidemia
PathBank:SMP0000139 3-Methylglutaconic Aciduria Type I
PathBank:SMP0000181 Ethylmalonic Encephalopathy
PathBank:SMP0000480 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0000198 Malonic Aciduria
PathBank:SMP0000502 Malonyl-CoA Decarboxylase Deficiency
PathBank:SMP0000522 3-Hydroxyisobutyric Aciduria
PathBank:SMP0000538 Carnitine Palmitoyl Transferase Deficiency I
PathBank:SMP0000542 Medium Chain Acyl-CoA Dehydrogenase Deficiency (MCAD)
PathBank:SMP0087327 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0087359 Mitochondrial Beta-Oxidation of Medium Chain Saturated Fatty Acids
PathBank:SMP0087519 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0087523 Mitochondrial Beta-Oxidation of Long Chain Saturated Fatty Acids
PathBank:SMP0063589 Fatty Acid Metabolism
PathBank:SMP0063639 Mitochondrial Beta-Oxidation of Long Chain Saturated Fatty Acids