EC: 1.2.1.47

4-trimethylammoniobutyraldehyde dehydrogenase (4-trimethylammoniobutanal:NAD+ 1-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P49189 [4-trimethylammoniobutyraldehyde dehydrogenase(Enzyme) wildtype fusion protein to maltose-binding protein]

4_Trimethylammoniobutanal + H2O + NAD+ -> NADH + H+ + 4_Trimethylammoniobutanoate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium pyrophosphate
PH: 9
Temperature: 37
uniprot:P49189 [4-trimethylammoniobutyraldehyde dehydrogenase(Enzyme) wildtype fusion protein to maltose-binding protein]

4_Trimethylammoniobutanal + H2O + NAD+ -> H+ + 4_Trimethylammoniobutanoate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium pyrophosphate
PH: 9
Temperature: 37
uniprot:Q9JLJ3 [4-trimethylammoniobutyraldehyde dehydrogenase(Enzyme) wildtype]

NAD+ + H2O + 4_Trimethylammoniobutanal -> H+ + 4_Trimethylammoniobutanoate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium pyrophosphate
PH: 9
Temperature: 37
uniprot:Q9JLJ3 [4-trimethylammoniobutyraldehyde dehydrogenase(Enzyme) wildtype fusion protein to maltose-binding protein]

4_Trimethylammoniobutanal + H2O + NAD+ -> H+ + NADH + 4_Trimethylammoniobutanoate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium pyrophosphate
PH: 9
Temperature: 37
uniprot:Q9JLJ3 [4-trimethylammoniobutyraldehyde dehydrogenase(Enzyme) wildtype]

4_Trimethylammoniobutanal + NAD+ + H2O -> H+ + 4_Trimethylammoniobutanoate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium pyrophosphate
PH: 9
Temperature: 37
uniprot:Q9JLJ3 [4-trimethylammoniobutyraldehyde dehydrogenase(Enzyme) wildtype fusion protein to maltose-binding protein]

NAD+ + H2O + 4_Trimethylammoniobutanal -> H+ + 4_Trimethylammoniobutanoate + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium pyrophosphate
PH: 9
Temperature: 37
uniprot:P49189 [4-trimethylammoniobutyraldehyde dehydrogenase(Enzyme) wildtype fusion protein to maltose-binding protein]

4_Trimethylammoniobutanal + H2O + NADP+ -> NADPH + 4_Trimethylammoniobutanoate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium pyrophosphate
PH: 9
Temperature: 37
uniprot:Q9JLJ3 [4-trimethylammoniobutyraldehyde dehydrogenase(Enzyme) wildtype]

NADP+ + 4_Trimethylammoniobutanal + H2O -> 4_Trimethylammoniobutanoate + NADPH + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium pyrophosphate
PH: 9
Temperature: 37
uniprot:Q9JLJ3 [4-trimethylammoniobutyraldehyde dehydrogenase(Enzyme) wildtype fusion protein to maltose-binding protein]

NADP+ + 4_Trimethylammoniobutanal + H2O -> NADPH + H+ + 4_Trimethylammoniobutanoate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M sodium pyrophosphate
PH: 9
Temperature: 37
uniprot:P49189 [4-trimethylammoniobutyraldehyde dehydrogenase(Enzyme) wildtype His6-tag]

H2O + NAD+ + 4_Trimethylammoniobutanal -> 4_Trimethylammoniobutanoate + NADH + H+		 (( (Vmax * A) ) / (Km + (A * (1 + (A / Ki))))) buffer: 150 mM sodium pyrophosphate
PH: 7.5
Temperature: 30
uniprot:P49189 [4-trimethylammoniobutyraldehyde dehydrogenase(Enzyme) wildtype His6-tag]

H2O + 4_Trimethylammoniobutanal + NAD+ -> H+ + NADH + 4_Trimethylammoniobutanoate		 (( (Vmax * A) ) / (Km + A)) buffer: 150 mM sodium pyrophosphate
PH: 7.5
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-6100 L-carnitine biosynthesis
BioCyc:HUMAN_PWY-6100 L-carnitine biosynthesis
BioCyc:MOUSE_PWY-6100 L-carnitine biosynthesis
BioCyc:MOUSE_PWY-2501 fatty acid α-oxidation
BioCyc:ARA_PWY-2 putrescine degradation IV
BioCyc:META_PWY1F-353 glycine betaine biosynthesis III (plants)
BioCyc:META_PWY-2 putrescine degradation IV
BioCyc:ARA_PWY1F-353 glycine betaine biosynthesis III (plants)
BioCyc:MOUSE_PWY-0 putrescine degradation III
BioCyc:MOUSE_PWY-3981 β-alanine biosynthesis I
PlantCyc:ARA_PWY1F-353 glycine betaine biosynthesis III (plants)
PlantCyc:PLANT_PWY1F-353 glycine betaine biosynthesis III (plants)
PlantCyc:PLANT_PWY-2 putrescine degradation IV
PlantCyc:ARA_PWY-2 putrescine degradation IV
Reactome:R-DRE-71291 Amino acid and derivative metabolism
Reactome:R-DRE-71262 Carnitine synthesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SSC-1430728 Metabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71262 Carnitine synthesis
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71262 Carnitine synthesis
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-RNO-71262 Carnitine synthesis
Reactome:R-SSC-71291 Amino acid and derivative metabolism
Reactome:R-SSC-71262 Carnitine synthesis
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP4159 GABA receptor signaling
PathBank:SMP0012047 Glycine Betaine Biosynthesis I
PathBank:SMP0000465 Carnitine Synthesis
PathBank:SMP0087187 Carnitine Synthesis
PathBank:SMP0012048 Glycine Betaine Biosynthesis II