EC: 1.13.11.27

4-hydroxyphenylpyruvate dioxygenase (4-hydroxyphenylpyruvate:oxygen oxidoreductase (hydroxylating, decarboxylating))

enzyme lambda metabolic reaction experiment
- [Sulcotrione]

O2 + 3_(4_Hydroxyphenyl)pyruvate -> CO2 + Homogentisate		 (( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Potassium phosphate, 5 units/µl Catalase, 15 mM GSH, 0.3 mM Dichlorophenolindophenol
PH: 7.3
Temperature: 30
uniprot:O23920 [4-hydroxyphenylpyruvate dioxygenase(Enzyme) wildtype]

O2 + 3_(4_Hydroxyphenyl)pyruvate -> Homogentisate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-acetate, 0.5 mM sodium ascorbate, 123 µM ammonium ferrous sulfate
PH: 6
Temperature: 30
uniprot:Q53586 [4-hydroxyphenylpyruvate dioxygenase(Enzyme) wildtype]

3_(4_Hydroxyphenyl)pyruvate + O2 -> CO2 + Homogentisate		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (Kia * Kmb) ) + (Kma * B)) ) + (Kmb * A)) ) + (A * B))) buffer: 20 mM HEPES, 1 mM Dithiothreitol, 5 µM ferrous ammonium sulfate, 5 µM ferrous ammonium sulfate
PH: 7
Temperature: 25
uniprot:Q53586 [Fe2+]

3_(4_Hydroxyphenyl)pyruvate + O2 -> CO2 + Homogentisate		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 20 mM HEPES, 1 mM Dithiothreitol
PH: 7
Temperature: 25
uniprot:Q53586 [4-hydroxyphenylpyruvate dioxygenase(Enzyme) wildtype]

O2 + 3_(4_Hydroxyphenyl)pyruvate -> Homogentisate + CO2		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 20 mM HEPES, 1 mM Dithiothreitol, 5 µM ferrous ammonium sulfate
PH: 7
Temperature: 25
uniprot:Q53586 [4-hydroxyphenylpyruvate dioxygenase(Enzyme) wildtype]

3_(4_Hydroxyphenyl)pyruvate + O2 -> CO2 + Homogentisate		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 20 mM HEPES, 1 mM Dithiothreitol, 5 µM ferrous ammonium sulfate
PH: 7
Temperature: 25
uniprot:P49429 [4-hydroxyphenylpyruvate dioxygenase(Enzyme) wildtype]

3_(4_Hydroxyphenyl)pyruvate + O2 -> Homogentisate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Potassium phosphate
PH: 7.5
Temperature: 37
uniprot:P49429 [4-hydroxyphenylpyruvate dioxygenase(Enzyme) wildtype]

O2 + 3_(4_Hydroxyphenyl)pyruvate -> CO2 + Homogentisate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Potassium phosphate
PH: 7.3
Temperature: 37
uniprot:P49429 [4-hydroxyphenylpyruvate dioxygenase(Enzyme) wildtype]

O2 + Phenylpyruvate -> CO2 + 2_Hydroxyphenylacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Potassium phosphate
PH: 7.3
Temperature: 37
uniprot:P49429 [4-hydroxyphenylpyruvate dioxygenase(Enzyme) wildtype]

3_(3,4_Dihydroxyphenyl)pyruvate + O2 -> 3,4_Dihydroxyphenylacetate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Potassium phosphate
PH: 7.3
Temperature: 37

Pathways

pathway id name
BioCyc:META_TYRFUMCAT-PWY L-tyrosine degradation I
BioCyc:HUMAN_TYRFUMCAT-PWY tyrosine degradation
BioCyc:META_PWY-6318 L-phenylalanine degradation IV (mammalian, via side chain)
BioCyc:MOUSE_TYRFUMCAT-PWY tyrosine degradation I
Reactome:R-RNO-8963684 Tyrosine catabolism
Reactome:R-HSA-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-BTA-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-MMU-8963684 Tyrosine catabolism
Reactome:R-RNO-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-HSA-8963684 Tyrosine catabolism
Reactome:R-BTA-8963684 Tyrosine catabolism
Reactome:R-MMU-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP4506 Tyrosine metabolism and related disorders
PathBank:SMP0000369 Tyrosinemia Type 2 (or Richner-Hanhart Syndrome)
PathBank:SMP0002457 Tyrosine Metabolism
PathBank:SMP0087450 Tyrosine Metabolism
PathBank:SMP0000206 Phenylketonuria
PathBank:SMP0000370 Tyrosinemia Type 3 (TYRO3)
PathBank:SMP0012043 Plastoquinol-9 Biosynthesis
PathBank:SMP0000008 Phenylalanine and Tyrosine Metabolism
PathBank:SMP0087218 Phenylalanine and Tyrosine Metabolism
PathBank:SMP0087235 Tyrosine Metabolism