EC: 1.1.1.105

all-trans-retinol dehydrogenase (NAD+) (all-trans retinol:NAD+ oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:O75452 [retinol dehydrogenase(Enzyme) wildtype RoDH-4]

Retinol + NAD+ -> all_trans_Retinal + H+ + NADH		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 90 mM potassium dihydrogen phosphate, 40 mM KCl, delipidated BSA in equimolar amounts to retinoids
PH: 7.4
Temperature: 37
uniprot:O75452 [Retinol]

5alpha_Androstane_3alpha_ol_17_one + NAD+ -> H+ + NADH + 5alpha_Androstane_3,17_dione		 (( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 90 mM Potassium phosphate, 40 mM KCl
PH: 7.3
Temperature: 37
uniprot:O75452 [retinol dehydrogenase(Enzyme) wildtype isoform RODH4]

5alpha_Androstane_3alpha_ol_17_one + NAD+ -> NADH + 5alpha_Androstane_3,17_dione + H+		 (( (Vmax * A) ) / (A + Km)) buffer: 90 mM Potassium phosphate, 40 mM KCl
PH: 7.3
Temperature: 37
uniprot:O75452 [retinol dehydrogenase(Enzyme) wildtype isoform RODH4]

NAD+ + 5alpha_Androstane_3alpha_ol_17_one -> NADH + H+ + 5alpha_Androstane_3,17_dione		 (( (Vmax * A) ) / (A + Km)) buffer: 90 mM Potassium phosphate, 40 mM KCl
PH: 7.3
Temperature: 37
uniprot:O75452 [retinol dehydrogenase(Enzyme) wildtype isoform RODH4]

H+ + 5alpha_Androstane_17beta_ol_3_one + NADPH -> NADP+ + 5alpha_Androstane_3alpha,17beta_diol		 (( (Vmax * A) ) / (A + Km)) buffer: 90 mM Potassium phosphate, 40 mM KCl
PH: 7.3
Temperature: 37
uniprot:O75452 [retinol dehydrogenase(Enzyme) wildtype isoform RODH4]

H+ + NADH + 5alpha_Androstane_17beta_ol_3_one -> NAD+ + 5alpha_Androstane_3alpha,17beta_diol		 (( (Vmax * A) ) / (A + Km)) buffer: 90 mM Potassium phosphate, 40 mM KCl
PH: 7.3
Temperature: 37
uniprot:O75452 [retinol dehydrogenase(Enzyme) wildtype isoform RODH4]

5alpha_Androstane_3alpha,17beta_diol + NAD+ -> H+ + NADH + 5alpha_Androstane_17beta_ol_3_one		 (( (Vmax * A) ) / (A + Km)) buffer: 90 mM Potassium phosphate, 40 mM KCl
PH: 7.3
Temperature: 37
uniprot:O75452 [retinol dehydrogenase(Enzyme) wildtype isoform RODH4]

5alpha_Androstane_17beta_ol_3_one + NADH + H+ -> 5alpha_Androstane_3alpha,17beta_diol + NAD+		 (( (Vmax * A) ) / (A + Km)) buffer: 90 mM Potassium phosphate, 40 mM KCl
PH: 7.3
Temperature: 37
uniprot:O75452 [retinol dehydrogenase(Enzyme) wildtype isoform RODH4]

5alpha_Androstane_3alpha_ol_17_one + NADP+ -> H+ + NADPH + 5alpha_Androstane_3,17_dione		 (( (Vmax * A) ) / (A + Km)) buffer: 90 mM Potassium phosphate, 40 mM KCl
PH: 7.3
Temperature: 37
uniprot:O75452 [Retinol-(cellular-retinol-binding-protein)]

NAD+ + 5alpha_Androstane_3alpha_ol_17_one -> NADH + 5alpha_Androstane_3,17_dione + H+		 (( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 90 mM Potassium phosphate, 40 mM KCl
PH: 7.3
Temperature: 37
uniprot:O75452 [13-cis-Retinol]

NAD+ + 5alpha_Androstane_3alpha_ol_17_one -> NADH + H+ + 5alpha_Androstane_3,17_dione		 (( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 90 mM Potassium phosphate, 40 mM KCl
PH: 7.3
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-6872 retinoate biosynthesis I
BioCyc:HUMAN_PWY66-401 superpathway of tryptophan utilization
BioCyc:META_PWY-6872 retinoate biosynthesis I
BioCyc:META_PWY66-21 ethanol degradation II
BioCyc:META_PWY-6313 serotonin degradation
BioCyc:META_PWY-6342 noradrenaline and adrenaline degradation
BioCyc:META_PWY-6861 the visual cycle I (vertebrates)
BioCyc:HUMAN_PWY-5451 acetone degradation I (to methylglyoxal)
BioCyc:HUMAN_PWY66-21 ethanol degradation II
BioCyc:META_PWY-6857 retinol biosynthesis
BioCyc:MOUSE_PWY66-21 ethanol degradation II (cytosol)
BioCyc:HUMAN_PWY-6857 retinol biosynthesis
BioCyc:HUMAN_PWY-6313 serotonin degradation
BioCyc:META_PWY-7466 acetone degradation III (to propane-1,2-diol)
BioCyc:HUMAN_PWY-6342 noradrenaline and adrenaline degradation
BioCyc:HUMAN_PWY-6861 the visual cycle I (vertebrates)
BioCyc:META_PWY-5451 acetone degradation I (to methylglyoxal)
BioCyc:MOUSE_PWY-5079 phenylalanine degradation III
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-HSA-5362517 Signaling by Retinoic Acid
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-MMU-162582 Signaling Pathways
Reactome:R-RNO-211945 Phase I - Functionalization of compounds
Reactome:R-RNO-71384 Ethanol oxidation
Reactome:R-RNO-162582 Signaling Pathways
Reactome:R-RNO-9006931 Signaling by Nuclear Receptors
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-9006931 Signaling by Nuclear Receptors
Reactome:R-MMU-5365859 RA biosynthesis pathway
Reactome:R-RNO-8964572 Lipid particle organization
Reactome:R-RNO-211859 Biological oxidations
Reactome:R-HSA-211945 Phase I - Functionalization of compounds
Reactome:R-HSA-71384 Ethanol oxidation
Reactome:R-HSA-5365859 RA biosynthesis pathway
Reactome:R-MMU-211945 Phase I - Functionalization of compounds
Reactome:R-MMU-71384 Ethanol oxidation
Reactome:R-MMU-9006931 Signaling by Nuclear Receptors
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-5362517 Signaling by Retinoic Acid
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8964572 Lipid particle organization
Reactome:R-HSA-162582 Signaling Pathways
Reactome:R-MMU-8964572 Lipid particle organization
Reactome:R-MMU-5362517 Signaling by Retinoic Acid
Reactome:R-RNO-5365859 RA biosynthesis pathway
WikiPathways:WP5188 Retinol metabolism
WikiPathways:WP4524 Alternative pathway of fetal androgen synthesis
PathBank:SMP0000429 Disulfiram Action Pathway
PathBank:SMP0000644 Celecoxib Metabolism Pathway
PathBank:SMP0087353 Retinol Metabolism
PathBank:SMP0120720 Vitamin A Deficiency
PathBank:SMP0000074 Retinol Metabolism
PathBank:SMP0000336 Vitamin A Deficiency
PathBank:SMP0087260 Retinol Metabolism
PathBank:SMP0000449 Ethanol Degradation
PathBank:SMP0000096 Celecoxib Action Pathway