EC: 6.3.4.4

adenylosuccinate synthase (IMP:L-aspartate ligase (GDP-forming))

enzyme lambda metabolic reaction experiment
uniprot:O24578 [adenylosuccinate synthase(Enzyme) wildtype]

L_Aspartate + IMP + GTP -> N6_(1,2_Dicarboxyethyl)_AMP + Phosphate + GDP		 (( (Vmax * C) ) / (KmC + C)) buffer: 50 mM N-[2-hydroxyethyl]piperazine-N’-3-propanesulfonic acid, 5 mM MgCl2, 1 mM Na2-EDTA, 5 mM DTT, 0.1% (v/v) glycerol, 10 mM potassium phosphate
PH: 7.5
Temperature: 22
uniprot:O24578 [adenylosuccinate synthase(Enzyme) wildtype]

GTP + L_Aspartate + IMP -> GDP + Phosphate + N6_(1,2_Dicarboxyethyl)_AMP		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM N-[2-hydroxyethyl]piperazine-N’-3-propanesulfonic acid, 5 mM MgCl2, 1 mM Na2-EDTA, 5 mM DTT, 0.1% (v/v) glycerol, 10 mM potassium phosphate
PH: 7.5
Temperature: 22
uniprot:O24578 [adenylosuccinate synthase(Enzyme) wildtype]

IMP + GTP + L_Aspartate -> Phosphate + GDP + N6_(1,2_Dicarboxyethyl)_AMP		 (( (Vmax * B) ) / (KmB + B)) buffer: 50 mM N-[2-hydroxyethyl]piperazine-N’-3-propanesulfonic acid, 5 mM MgCl2, 1 mM Na2-EDTA, 5 mM DTT, 0.1% (v/v) glycerol, 10 mM potassium phosphate
PH: 7.5
Temperature: 22
uniprot:Q02787 [adenylosuccinate synthase(Enzyme) wildtype]

GTP + L_Aspartate + IMP -> N6_(1,2_Dicarboxyethyl)_AMP + Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 140 mM glycine, 6 mM MgCl2, 0.6 mM phosphoenolpyruvate, 30 U/ml pyruvate kinase
PH: 8
Temperature: 37
uniprot:Q02787 [adenylosuccinate synthase(Enzyme) wildtype]

GTP + IMP + L_Aspartate -> N6_(1,2_Dicarboxyethyl)_AMP + Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 140 mM glycine, 6 mM MgCl2, 0.6 mM phosphoenolpyruvate, 30 U/ml pyruvate kinase
PH: 8
Temperature: 37
uniprot:Q02787 [adenylosuccinate synthase(Enzyme) wildtype]

L_Aspartate + GTP + IMP -> N6_(1,2_Dicarboxyethyl)_AMP + Phosphate + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 140 mM glycine, 6 mM MgCl2, 0.6 mM phosphoenolpyruvate, 30 U/ml pyruvate kinase
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:META_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:YEAST_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:YEAST_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:MTBCDC1551_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:ECOL199310_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:CAULO_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:SYNEL_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:SYNEL_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:CAULONA1000_PWY-841 purine nucleotides de novo biosynthesis II
BioCyc:BTHE_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:BTHE_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:CORYNE_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:HUMAN_PWY66-409 superpathway of purine nucleotide salvage
BioCyc:VCHO_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:VCHO_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:MTBCDC1551_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:MTBCDC1551_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:HPY_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:ECOO157_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:ECOL199310_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:CAULO_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:ANTHRA_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:PCHR_PWY-841 purine nucleotides de novo biosynthesis II
BioCyc:ECOL316407_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:ECO_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:YEAST_PWY-6126 adenosine nucleotides de novo biosynthesis
BioCyc:ARA_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:VCHO_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:MTBH37RV_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:MTBH37RV_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:HPY_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:ECOL199310_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:CAULO_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:ANTHRA_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:AGRO_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:BSUB_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:HUMAN_PWY-841 purine nucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:META_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:ECOO157_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:ANTHRA_DENOVOPURINE2-PWY superpathway of purine nucleotides de novo biosynthesis II
BioCyc:ANTHRA_PWY-6126 superpathway of adenosine nucleotides de novo biosynthesis II
BioCyc:ANTHRA_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:BSUB_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:SYNEL_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:SYNEL_DENOVOPURINE2-PWY superpathway of purine nucleotides de novo biosynthesis II
BioCyc:PDIF272563_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:PDIF272563_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:PDIF272563_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:BTHE_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:CORYNE_PWY-7219 adenosine ribonucleotides de novo biosynthesis
PathBank:SMP0000809 Aspartate Metabolism
PathBank:SMP0000927 Purine Nucleotides De Novo Biosynthesis
PathBank:SMP0002047 Purine Nucleotides De Novo Biosynthesis 2
PlantCyc:PLANT_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
PlantCyc:PLANT_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
PlantCyc:ARA_PWY-7219 adenosine ribonucleotides de novo biosynthesis
PlantCyc:PLANT_PWY-7219 adenosine ribonucleotides de novo biosynthesis
PlantCyc:ARA_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
PlantCyc:ARA_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
Reactome:R-GGA-1660598 Metabolism
Reactome:R-GGA-421203 De novo synthesis of AMP
Reactome:R-GGA-419470 Nucleotide metabolism
Reactome:R-GGA-419474 Purine metabolism
WikiPathways:WP4792 Purine metabolism
WikiPathways:WP4224 Purine metabolism and related disorders
PathBank:SMP0120448 Adenosine Deaminase Deficiency
PathBank:SMP0120452 AICA-Ribosiduria
PathBank:SMP0120508 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0120532 Molybdenum Cofactor Deficiency
PathBank:SMP0120536 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0120577 Xanthinuria Type I
PathBank:SMP0120601 Mitochondrial DNA Depletion Syndrome
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0087165 Aspartate Metabolism
PathBank:SMP0063668 Purine Metabolism
PathBank:SMP0120600 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0121271 Aspartate Metabolism
PathBank:SMP0121307 Purine Nucleotides De Novo Biosynthesis
PathBank:SMP0122223 Purine Nucleotides De Novo Biosynthesis 2
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0120449 Adenylosuccinate Lyase Deficiency
PathBank:SMP0120461 Canavan Disease
PathBank:SMP0120489 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0120497 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0120578 Xanthinuria Type II
PathBank:SMP0120602 Myoadenylate Deaminase Deficiency
PathBank:SMP0000067 Aspartate Metabolism
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000175 Canavan Disease
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0120502 Hypoacetylaspartia
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000192 Hypoacetylaspartia
PathBank:SMP0000427 Azathioprine Action Pathway
PathBank:SMP0087239 Purine Metabolism
PathBank:SMP0063597 Aspartate Metabolism