EC: 4.6.1.1

adenylate cyclase (ATP diphosphate-lyase (cyclizing; 3',5'-cyclic-AMP-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q553Y7 [GTP-gamma-S]

ATP -> Diphosphate + 3',5'_Cyclic_AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 40 mm Hepes, 3 mm EGTA, 3 mm Mg2+, 10 mm dithiothreitol
PH: 7
Temperature: 36
uniprot:Q553Y7 [adenylate cyclase(Enzyme) mutant DdGCAkqd (E440K, S502Q, H504D)]

ATP -> Diphosphate + 3',5'_Cyclic_AMP		 (( (Vmax * S) ) / (S + Km)) buffer: 40 mm Hepes, 3 mm EGTA, 3 mm Mg2+, 10 mm dithiothreitol
PH: 7
Temperature: 36
- [Mn2+]

ATP -> Diphosphate + 3',5'_Cyclic_AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 45 mM Tris-HCl, 1 mM cAMP (3H-labeled), 1.4 mM EDTA, 1 mM beta-mercaptoethanol, regenerating system for ATP (0.02 mg/ml myokinase, 0.2 mg/ml creatine kinase, 20 mM creatine phosphate)
PH: 7.5
Temperature: 35
- [Mn2+]

ATP -> 3',5'_Cyclic_AMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 45 mM Tris-HCl, 1 mM cAMP (3H-labeled), 1.4 mM EDTA, 1 mM beta-mercaptoethanol, regenerating system for ATP (0.02 mg/ml myokinase, 0.2 mg/ml creatine kinase, 20 mM creatine phosphate)
PH: 7.5
Temperature: 35
- [Mn2+]

ATP -> Diphosphate + 3',5'_Cyclic_AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 1.4 mM EDTA, 1 mM beta-mercaptoethanol, regenerating system for ATP (0.02 mg/ml myokinase, 0.2 mg/ml creatine kinase, 20 mM creatine phosphate)
PH: 7.5
Temperature: 35
- [Mn2+]

ATP -> Diphosphate + 3',5'_Cyclic_AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 1.4 mM EDTA, 1 mM beta-mercaptoethanol, regenerating system for ATP (0.02 mg/ml myokinase, 0.2 mg/ml creatine kinase, 20 mM creatine phosphate)
PH: 7.5
Temperature: 35
- [Mn2+]

ATP -> 3',5'_Cyclic_AMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM EDTA, 1 mM 3H-cAMP, ATP regenerating system (25 mM phosphocreatine, 25 U/ml creatine phosphokinase
PH: 8.2
Temperature: 30
- [Mn2+]

ATP -> 3',5'_Cyclic_AMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM EDTA, 1 mM 3H-cAMP, ATP regenerating system (25 mM phosphocreatine, 25 U/ml creatine phosphokinase
PH: 8.2
Temperature: 30
- [Mg2+]

ATP -> 3',5'_Cyclic_AMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM EDTA, 1 mM 3H-cAMP, ATP regenerating system (25 mM phosphocreatine, 25 U/ml creatine phosphokinase
PH: 8.2
Temperature: 30
- [Mg2+]

ATP -> Diphosphate + 3',5'_Cyclic_AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM EDTA, 1 mM 3H-cAMP, ATP regenerating system (25 mM phosphocreatine, 25 U/ml creatine phosphokinase
PH: 8.2
Temperature: 30
- [adenylate cyclase(Enzyme) wildtype AC2]

ATP -> Diphosphate + 3',5'_Cyclic_AMP		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM NaHEPES, 2 mM EDTA, 0.5 mM Isobutylmethylxanthine, 5 mM Mg2+
PH: 8
Temperature: 32
- [12-O-Tetradecanoylphorbol 13-acetate]

ATP -> Diphosphate + 3',5'_Cyclic_AMP		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM NaHEPES, 2 mM EDTA, 0.5 mM Isobutylmethylxanthine, 5 mM Mg2+
PH: 8
Temperature: 32

Pathways

pathway id name
BioCyc:MOUSE_PWY3DJ-1 cyclic AMP biosynthesis
Reactome:R-HSA-9824439 Bacterial Infection Pathways
Reactome:R-HSA-9709957 Sensory Perception
Reactome:R-SPO-6803207 TP53 Regulates Transcription of Caspase Activators and Caspases
Reactome:R-SPO-9652282 Drug-mediated inhibition of ERBB2 signaling
Reactome:R-SPO-1227986 Signaling by ERBB2
Reactome:R-SPO-199418 Negative regulation of the PI3K/AKT network
Reactome:R-DDI-418594 G alpha (i) signalling events
Reactome:R-DDI-112043 PLC beta mediated events
Reactome:R-DDI-111931 PKA-mediated phosphorylation of CREB
Reactome:R-HSA-381753 Olfactory Signaling Pathway
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-5339562 Uptake and actions of bacterial toxins
Reactome:R-SPO-3700989 Transcriptional Regulation by TP53
Reactome:R-SPO-9006925 Intracellular signaling by second messengers
Reactome:R-DDI-372790 Signaling by GPCR
Reactome:R-DDI-112040 G-protein mediated events
Reactome:R-DDI-111933 Calmodulin induced events
Reactome:R-DDI-170670 Adenylate cyclase inhibitory pathway
Reactome:R-DDI-418597 G alpha (z) signalling events
Reactome:R-DDI-9006925 Intracellular signaling by second messengers
Reactome:R-DDI-991365 Activation of GABAB receptors
Reactome:R-SPO-5223345 Miscellaneous transport and binding events
Reactome:R-SPO-212436 Generic Transcription Pathway
Reactome:R-DDI-111885 Opioid Signalling
Reactome:R-DDI-111996 Ca-dependent events
Reactome:R-DDI-163615 PKA activation
Reactome:R-DDI-1489509 DAG and IP3 signaling
Reactome:R-DDI-112316 Neuronal System
Reactome:R-DDI-977443 GABA receptor activation
Reactome:R-SPO-382551 Transport of small molecules
Reactome:R-SPO-74160 Gene expression (Transcription)
Reactome:R-SPO-162582 Signaling Pathways
Reactome:R-SPO-1257604 PI3K/AKT Signaling
Reactome:R-DDI-162582 Signaling Pathways
Reactome:R-DDI-388396 GPCR downstream signalling
Reactome:R-DDI-111997 CaM pathway
Reactome:R-DDI-170660 Adenylate cyclase activating pathway
Reactome:R-DDI-112315 Transmission across Chemical Synapses
Reactome:R-DDI-112314 Neurotransmitter receptors and postsynaptic signal transmission
Reactome:R-DDI-977444 GABA B receptor activation
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-HSA-5210891 Uptake and function of anthrax toxins
Reactome:R-SPO-73857 RNA Polymerase II Transcription
Reactome:R-SPO-5633008 TP53 Regulates Transcription of Cell Death Genes
Reactome:R-SPO-9006934 Signaling by Receptor Tyrosine Kinases
WikiPathways:WP4589 Major receptors targeted by epinephrine and norepinephrine
PathBank:SMP0108125 Excitatory Neural Signalling Through 5-HTR 6 and Serotonin
PathBank:SMP0108137 Intracellular Signalling Through LHCGR Receptor and Luteinizing Hormone/Choriogonadotropin
PathBank:SMP0108193 Lysophosphatidic Acid LPA2 Signalling
PathBank:SMP0108221 Vasopressin Regulation of Water Homeostasis
PathBank:SMP0108229 Lysophosphatidic Acid LPA4 Signalling
PathBank:SMP0108237 Ion Channels and Their Functional Role in Vascular Endothelium
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000308 Dopamine Activation of Neurological Reward System
PathBank:SMP0000333 Intracellular Signalling Through FSH Receptor and Follicle Stimulating Hormone
PathBank:SMP0000354 Intracellular Signalling Through Prostacyclin Receptor and Prostacyclin
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0063753 Lysophosphatidic Acid LPA2 Signalling
PathBank:SMP0063759 Lysophosphatidic Acid LPA6 Signalling
PathBank:SMP0107986 Intracellular Signalling Through PGD2 receptor and Prostaglandin D2
PathBank:SMP0108120 Corticotropin Activation of Cortisol Production
PathBank:SMP0108124 Excitatory Neural Signalling Through 5-HTR 7 and Serotonin
PathBank:SMP0108196 Lysophosphatidic Acid LPA4 Signalling
PathBank:SMP0108228 Lysophosphatidic Acid LPA3 Signalling
PathBank:SMP0108232 Activation of cAMP-dependent protein kinase, PKA
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000322 Vasopressin Regulation of Water Homeostasis
PathBank:SMP0000343 Intracellular Signalling Through PGD2 receptor and Prostaglandin D2
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0063746 Lysophosphatidic Acid LPA1 Signalling
PathBank:SMP0063757 Lysophosphatidic Acid LPA5 Signalling
PathBank:SMP0107988 Intracellular Signalling Through Prostacyclin Receptor and Prostacyclin
PathBank:SMP0108122 Excitatory Neural Signalling Through 5-HTR 4 and Serotonin
PathBank:SMP0108130 Intracellular Signalling Through Adenosine Receptor A2b and Adenosine
PathBank:SMP0108134 Vasopressin Regulation of Water Homeostasis
PathBank:SMP0108194 Lysophosphatidic Acid LPA3 Signalling
PathBank:SMP0108198 Lysophosphatidic Acid LPA5 Signalling
PathBank:SMP0108222 Intracellular Signalling Through FSH Receptor and Follicle Stimulating Hormone
PathBank:SMP0108226 Lysophosphatidic Acid LPA1 Signalling
PathBank:SMP0108230 Lysophosphatidic Acid LPA5 Signalling
PathBank:SMP0121018 Pancreas Function - Beta Cell
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000335 Intracellular Signalling Through Histamine H2 Receptor and Histamine
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0063756 Lysophosphatidic Acid LPA4 Signalling
PathBank:SMP0063778 Ion Channels and Their Functional Role in Vascular Endothelium
PathBank:SMP0107985 Dopamine Activation of Neurological Reward System
PathBank:SMP0108127 Intracellular Signalling Through Adenosine Receptor A2a and Adenosine
PathBank:SMP0108135 Intracellular Signalling Through FSH Receptor and Follicle Stimulating Hormone
PathBank:SMP0108191 Lysophosphatidic Acid LPA1 Signalling
PathBank:SMP0108199 Lysophosphatidic Acid LPA6 Signalling
PathBank:SMP0108227 Lysophosphatidic Acid LPA2 Signalling
PathBank:SMP0108231 Lysophosphatidic Acid LPA6 Signalling
PathBank:SMP0120925 GnRH Signaling Pathway
PathBank:SMP0120949 GnRH Signaling Pathway
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000427 Azathioprine Action Pathway
PathBank:SMP0000321 Intracellular Signalling Through Adenosine Receptor A2b and Adenosine
PathBank:SMP0000338 Intracellular Signalling Through LHCGR Receptor and Luteinizing Hormone/Choriogonadotropin
PathBank:SMP0063755 Lysophosphatidic Acid LPA3 Signalling
PathBank:SMP0063764 Activation of cAMP-dependent protein kinase, PKA
PathBank:SMP0063811 GnRH Signaling Pathway