EC: 4.3.1.17

L-serine ammonia-lyase (L-serine ammonia-lyase (pyruvate-forming))

enzyme lambda metabolic reaction experiment
uniprot:B2DFG5 [L-serine ammonia-lyase(Enzyme) wildtype]

L_Serine -> NH3 + Pyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
uniprot:P09367 [Pyridoxal phosphate]

L_Serine -> Pyruvate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 mM Potassium phosphate, 1 mM EDTA, 0.15 M KCl, 1 mM Dithiothreitol, 4 mM MgCl2, 0.1 M NaF
PH: 7.2
Temperature: 37
uniprot:P09367-2 [L-serine ammonia-lyase(Enzyme) wildtype isoform 2]

L_Serine -> NH3 + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37
uniprot:P09367-1 [L-serine ammonia-lyase(Enzyme) wildtype isoform 1]

L_Serine -> NH3 + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37
uniprot:P09367 [Pyridoxal phosphate]

L_Serine -> NH3 + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 mM Potassium phosphate, 1 mM EDTA, 0.15 M KCl, 1 mM Dithiothreitol, 4 mM MgCl2, 0.1 M NaF
PH: 7.2
Temperature: 37
uniprot:P09367 [L-serine ammonia-lyase(Enzyme) wildtype isoenzyme 1]

L_Threonine -> NH3 + 2_Oxobutyrate		 (( (Vmax * S) ) / (Km + S)) buffer: Tris-HCl
PH: 8.6
Temperature: 36
uniprot:P09367 [L-serine ammonia-lyase(Enzyme) wildtype isoenzyme 1]

L_Serine -> NH3 + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: Tris-HCl
PH: 8.6
Temperature: 36
uniprot:P09367 [L-serine ammonia-lyase(Enzyme) wildtype isoenzyme 2]

L_Threonine -> 2_Oxobutyrate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: Tris-HCl
PH: 8.6
Temperature: 36
uniprot:P09367 [L-serine ammonia-lyase(Enzyme) wildtype isoenzyme 2]

L_Serine -> Pyruvate + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: Tris-HCl
PH: 8.6
Temperature: 36

Pathways

pathway id name
BioCyc:HUMAN_SERDEG-PWY L-serine degradation
BioCyc:MTBH37RV_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:MTBH37RV_PWY-3001 superpathway of L-isoleucine biosynthesis I
BioCyc:ECOL316407_SERDEG-PWY L-serine degradation
BioCyc:META_PWY-6196 D-serine metabolism
BioCyc:META_PWY66-428 L-threonine degradation V
BioCyc:ARA_PWY-6196 D-serine metabolism
BioCyc:MOUSE_SERDEG-PWY L-serine degradation
BioCyc:HUMAN_PWY66-428 threonine degradation
BioCyc:MTBH37RV_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:MTBCDC1551_SERDEG-PWY L-serine degradation
BioCyc:ECOL316407_ILEUSYN-PWY isoleucine biosynthesis I (from threonine)
BioCyc:ECOL316407_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:ECOL316407_PWY-5437 threonine degradation I
BioCyc:YEAST_SERDEG-PWY L-serine degradation
BioCyc:ARA_PWY0-1535 D-serine degradation
BioCyc:MTBH37RV_SERDEG-PWY L-serine degradation
BioCyc:MOUSE_PWY-3661 glycine betaine degradation
BioCyc:ECOL316407_THREOCAT-PWY superpathway of threonine metabolism
PathBank:SMP0000829 Serine Biosynthesis and Metabolism
PathBank:SMP0002113 D-Serine Degradation
PlantCyc:ARA_PWY-6196 D-serine metabolism
PlantCyc:ARA_PWY0-1535 D-serine degradation
PlantCyc:PLANT_PWY-6196 D-serine metabolism
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-DDI-8849175 Threonine catabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SCE-71291 Amino acid and derivative metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-HSA-977347 Serine metabolism
Reactome:R-HSA-8849175 Threonine catabolism
Reactome:R-MMU-977347 Serine metabolism
Reactome:R-MMU-8849175 Threonine catabolism
Reactome:R-RNO-977347 Serine metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SPO-71291 Amino acid and derivative metabolism
Reactome:R-DDI-977347 Serine metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-SPO-977347 Serine metabolism
Reactome:R-BTA-977347 Serine metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-RNO-8849175 Threonine catabolism
Reactome:R-SCE-977347 Serine metabolism
Reactome:R-SCE-8849175 Threonine catabolism
WikiPathways:WP4688 Serine metabolism
PathBank:SMP0120694 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0120754 Non-Ketotic Hyperglycinemia
PathBank:SMP0120770 Hyperglycinemia, Non-Ketotic
PathBank:SMP0000009 Ammonia Recycling
PathBank:SMP0000242 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0000223 Non-Ketotic Hyperglycinemia
PathBank:SMP0000484 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0087177 Ammonia Recycling
PathBank:SMP0087262 Threonine and 2-Oxobutanoate Degradation
PathBank:SMP0087338 Glycine and Serine Metabolism
PathBank:SMP0063579 Ammonia Recycling
PathBank:SMP0120653 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0120741 Sarcosinemia
PathBank:SMP0120475 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0000004 Glycine and Serine Metabolism
PathBank:SMP0000721 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0002323 Serine Metabolism
PathBank:SMP0087245 Glycine and Serine Metabolism
PathBank:SMP0063678 Threonine and 2-Oxobutanoate Degradation
PathBank:SMP0120872 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0120521 Sarcosinemia
PathBank:SMP0000179 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0000485 Hyperglycinemia, Non-Ketotic
PathBank:SMP0002443 Serine Metabolism
PathBank:SMP0120695 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0120474 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0120534 Non-Ketotic Hyperglycinemia
PathBank:SMP0120550 Hyperglycinemia, Non-Ketotic
PathBank:SMP0000452 Threonine and 2-Oxobutanoate Degradation
PathBank:SMP0000244 Sarcosinemia
PathBank:SMP0063626 Glycine and Serine Metabolism