EC: 4.2.3.3

methylglyoxal synthase (glycerone-phosphate phosphate-lyase (methylglyoxal-forming))

enzyme lambda metabolic reaction experiment
uniprot:P0A731 [2-Phosphoglycolate]

Glycerone_phosphate -> Phosphate + Methylglyoxal		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 2 U Glyoxalase I
PH: 7
Temperature: 25
uniprot:P0A731 [methylglyoxal synthase(Enzyme) mutant H98Q]

Glycerone_phosphate -> Methylglyoxal + Phosphate		 (( (( (kcat * E) ) * (A ^ h)) ) / (( (Km ^ (1 / h)) ) + (A ^ h))) buffer: 50 mM Imidazole, 15 mM Glutathione, 2 U Glyoxalase I
PH: 7
Temperature: 25
uniprot:P0A731 [methylglyoxal synthase(Enzyme) wildtype]

Glycerone_phosphate -> Phosphate + Methylglyoxal		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 2 U Glyoxalase I
PH: 6
Temperature: 25
uniprot:P0A731 [methylglyoxal synthase(Enzyme) wildtype]

Glycerone_phosphate -> Phosphate + Methylglyoxal		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Imidazole, 1.65 mM Glutathione, Glyoxalase I (EC 4.4.1.5)
PH: 7
Temperature: 30
uniprot:P0A731 [Phosphoglycolohydroxamate]

Glycerone_phosphate -> Methylglyoxal + Phosphate		 (Vmax * (( (( (S / Kd_S) ) * (( (( (1 + (S / Kd_S)) ) + (I / Kd_I)) ) ^ (n - 1))) ) / (L + (( (( (1 + (S / Kd_S)) ) + (I / Kd_I)) ) ^ n)))) buffer: 50 mM Imidazole, 1.65 mM Glutathione, Glyoxalase I (EC 4.4.1.5)
PH: 7
Temperature: 30
uniprot:P0A731 [Phosphoglycolohydroxamate]

Glycerone_phosphate -> Phosphate + Methylglyoxal		 (( (v0 / (2 * E)) ) * (( (( (E - I) ) - Ki) ) + (( (( (( (( (E - I) ) - Ki) ) ^ 2) ) + (( (4 * E) ) * Ki)) ) ^ 0.5))) buffer: 50 mM Imidazole, 1.65 mM Glutathione, Glyoxalase I (EC 4.4.1.5)
PH: 7
Temperature: 30
uniprot:P0A731 [methylglyoxal synthase(Enzyme) mutant D71N]

Glycerone_phosphate -> Methylglyoxal + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [methylglyoxal synthase(Enzyme) mutant D71E]

Glycerone_phosphate -> Phosphate + Methylglyoxal		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [methylglyoxal synthase(Enzyme) mutant D20E]

Glycerone_phosphate -> Methylglyoxal + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [methylglyoxal synthase(Enzyme) wildtype]

Glycerone_phosphate -> Methylglyoxal + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [methylglyoxal synthase(Enzyme) mutant D91E]

Glycerone_phosphate -> Phosphate + Methylglyoxal		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [methylglyoxal synthase(Enzyme) mutant D91E]

Glycerone_phosphate -> Methylglyoxal + Phosphate		 (( (( (kcat * E) ) * (A ^ h)) ) / (Km + (A ^ h))) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [methylglyoxal synthase(Enzyme) wildtype]

Glycerone_phosphate -> Methylglyoxal + Phosphate		 (( (( (kcat * E) ) * (A ^ h)) ) / (Km + (A ^ h))) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [methylglyoxal synthase(Enzyme) mutant D20E]

Glycerone_phosphate -> Methylglyoxal + Phosphate		 (( (( (kcat * E) ) * (A ^ h)) ) / (Km + (A ^ h))) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [methylglyoxal synthase(Enzyme) mutant D101N]

Glycerone_phosphate -> Methylglyoxal + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [methylglyoxal synthase(Enzyme) mutant D101E]

Glycerone_phosphate -> Phosphate + Methylglyoxal		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [methylglyoxal synthase(Enzyme) mutant D71N]

Glycerone_phosphate -> Phosphate + Methylglyoxal		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [methylglyoxal synthase(Enzyme) mutant D71E]

Glycerone_phosphate -> Phosphate + Methylglyoxal		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [methylglyoxal synthase(Enzyme) mutant D91N]

Glycerone_phosphate -> Methylglyoxal + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [methylglyoxal synthase(Enzyme) mutant D101N]

Glycerone_phosphate -> Methylglyoxal + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [2-Phosphoglycolate]

Glycerone_phosphate -> Methylglyoxal + Phosphate		 (( (( (( (kcat * E) ) * (A / KdA)) ) * (( (( (1 + (A / KdA)) ) + (I / Ki)) ) ^ n)) ) - (1 / (L + (( (( (1 + (A / KdA)) ) + (I / Ki)) ) ^ n)))) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [2-Phosphoglycolate]

Glycerone_phosphate -> Phosphate + Methylglyoxal		 (( (( (( (kcat * E) ) * (A / KdA)) ) * (( (( (1 + (A / KdA)) ) + (I / Ki)) ) ^ n)) ) - (1 / (L + (( (( (1 + (A / KdA)) ) + (I / Ki)) ) ^ n)))) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [2-Phosphoglycolate]

Glycerone_phosphate -> Methylglyoxal + Phosphate		 (( (( (( (kcat * E) ) * (A / KdA)) ) * (( (( (1 + (A / KdA)) ) + (I / Ki)) ) ^ n)) ) - (1 / (L + (( (( (1 + (A / KdA)) ) + (I / Ki)) ) ^ n)))) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [2-Phosphoglycolate]

Glycerone_phosphate -> Phosphate + Methylglyoxal		 (( (( (kcat * E) ) * A) ) / (( (Km * (1 + (I / Kii))) ) + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [2-Phosphoglycolate]

Glycerone_phosphate -> Methylglyoxal + Phosphate		 (( (( (kcat * E) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [2-Phosphoglycolate]

Glycerone_phosphate -> Phosphate + Methylglyoxal		 (( (( (kcat * E) ) * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [methylglyoxal synthase(Enzyme) wildtype]

Glycerone_phosphate -> Methylglyoxal + Phosphate		 (( (( (kcat * E) ) * (A ^ h)) ) / (Km + (A ^ h))) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30
uniprot:P0A731 [methylglyoxal synthase(Enzyme) mutant D101E]

Glycerone_phosphate -> Methylglyoxal + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Imidazole, 15 mM Glutathione, 3.3 U/ml Yeast glyoxalase I
PH: 7
Temperature: 30

Pathways

pathway id name
BioCyc:HUMAN_PWY66-400 glycolysis
BioCyc:META_PWY66-373 sucrose degradation V (sucrose α-glucosidase)
BioCyc:META_ANAGLYCOLYSIS-PWY glycolysis III (from glucose)
BioCyc:MOUSE_PWY66-373 sucrose degradation V (mammalian)
BioCyc:MOUSE_GLYCOLYSIS glycolysis I
BioCyc:HUMAN_PWY66-399 gluconeogenesis
BioCyc:META_ANAEROFRUCAT-PWY homolactic fermentation
BioCyc:HUMAN_PWY66-407 superpathway of conversion of glucose to acetyl CoA and entry into the TCA cycle
BioCyc:META_PWY66-399 gluconeogenesis III
BioCyc:MOUSE_P341-PWY glycolysis V (Pyrococcus)
BioCyc:HUMAN_PWY66-373 sucrose degradation
BioCyc:MOUSE_ANAGLYCOLYSIS-PWY glycolysis III
Reactome:R-CEL-70263 Gluconeogenesis
Reactome:R-DRE-70171 Glycolysis
Reactome:R-GGA-70171 Glycolysis
Reactome:R-GGA-1660598 Metabolism
Reactome:R-GGA-352875 Gluconeogenesis
Reactome:R-HSA-70263 Gluconeogenesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-XTR-70171 Glycolysis
Reactome:R-HSA-70171 Glycolysis
Reactome:R-CEL-71387 Carbohydrate metabolism
Reactome:R-CEL-70326 Glucose metabolism
Reactome:R-DRE-70263 Gluconeogenesis
Reactome:R-GGA-70263 Gluconeogenesis
Reactome:R-GGA-352882 Glycolysis
Reactome:R-HSA-71387 Carbohydrate metabolism
Reactome:R-HSA-70326 Glucose metabolism
Reactome:R-MMU-70171 Glycolysis
Reactome:R-XTR-70263 Gluconeogenesis
Reactome:R-CEL-70171 Glycolysis
Reactome:R-DRE-1430728 Metabolism
Reactome:R-GGA-1430728 Metabolism
Reactome:R-GGA-353098 Carbohydrate metabolism
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-MMU-70326 Glucose metabolism
Reactome:R-XTR-1430728 Metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DRE-71387 Carbohydrate metabolism
Reactome:R-DRE-70326 Glucose metabolism
Reactome:R-GGA-71387 Carbohydrate metabolism
Reactome:R-GGA-70326 Glucose metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-MMU-70263 Gluconeogenesis
Reactome:R-XTR-71387 Carbohydrate metabolism
Reactome:R-XTR-70326 Glucose metabolism
PathBank:SMP0120488 Glycogen Storage Disease Type 1A (GSD1A) or Von Gierke Disease
PathBank:SMP0120625 Fructosuria
PathBank:SMP0120637 Glycogenosis, Type IB
PathBank:SMP0120645 Glycogenosis, Type IA. Von Gierke Disease
PathBank:SMP0000064 Fructose and Mannose Degradation
PathBank:SMP0000374 Glycogen Storage Disease Type 1A (GSD1A) or Von Gierke Disease
PathBank:SMP0000560 Phosphoenolpyruvate Carboxykinase Deficiency 1 (PEPCK1)
PathBank:SMP0087197 Fructose and Mannose Degradation
PathBank:SMP0120657 Fructose Intolerance, Hereditary
PathBank:SMP0120596 Glycogenosis, Type VII. Tarui Disease
PathBank:SMP0120624 Phosphoenolpyruvate Carboxykinase Deficiency 1 (PEPCK1)
PathBank:SMP0120636 Fanconi-Bickel Syndrome
PathBank:SMP0000563 Triosephosphate Isomerase Deficiency
PathBank:SMP0000725 Fructose Intolerance, Hereditary
PathBank:SMP0063617 Gluconeogenesis
PathBank:SMP0120626 Fructose-1,6-diphosphatase Deficiency
PathBank:SMP0120638 Glycogenosis, Type IC
PathBank:SMP0000128 Gluconeogenesis
PathBank:SMP0000561 Fructosuria
PathBank:SMP0000573 Glycogenosis, Type IB
PathBank:SMP0000581 Glycogenosis, Type IA. Von Gierke Disease
PathBank:SMP0087223 Gluconeogenesis
PathBank:SMP0063478 Glycolysis
PathBank:SMP0063614 Fructose and Mannose Degradation
PathBank:SMP0120627 Triosephosphate Isomerase Deficiency
PathBank:SMP0000562 Fructose-1,6-diphosphatase Deficiency
PathBank:SMP0000574 Glycogenosis, Type IC
PathBank:SMP0087222 Glycolysis