EC: 1.13.11.11

tryptophan 2,3-dioxygenase (L-tryptophan:oxygen 2,3-oxidoreductase (decyclizing))

enzyme lambda metabolic reaction experiment
uniprot:Q1LK00 [Fe2+]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.1 mM L-ascorbate
PH: 7
Temperature: 22
uniprot:Q8PDA8 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

5_Methyltryptophan + O2 -> 5_Methyl_N_formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + 5_Methyltryptophan -> 5_Methyl_N_formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + 5_Fluorotryptophan -> 5_Fluoro_N_formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

6_Methyltryptophan + O2 -> 6_Methyl_N_formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

6_Methyltryptophan + O2 -> 6_Methyl_N_formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + 6_Fluorotryptophan -> 6_Fluoro_N_formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 6
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 6
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 6.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 6.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 6
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + 5_Methyltryptophan -> 5_Methyl_N_formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

5_Fluorotryptophan + O2 -> 5_Fluoro_N_formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + 6_Fluorotryptophan -> 6_Fluoro_N_formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

6_Methyltryptophan + O2 -> 6_Methyl_N_formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM potassium phosphate 10 µM methylene blue, 10 µg/ml catalase, 20 mM L-ascorbate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM potassium phosphate 10 µM methylene blue, 10 µg/ml catalase, 20 mM L-ascorbate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

5_Fluorotryptophan + O2 -> 5_Fluoro_N_formylkynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM potassium phosphate 10 µM methylene blue, 10 µg/ml catalase, 20 mM L-ascorbate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + 6_Fluorotryptophan -> 6_Fluoro_N_formylkynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM potassium phosphate 10 µM methylene blue, 10 µg/ml catalase, 20 mM L-ascorbate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM phosphate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 100 mM phosphate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 8.3
Temperature: 25
uniprot:Q8PDA8 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM potassium phosphate 10 µM methylene blue, 10 µg/ml catalase, 20 mM L-ascorbate
PH: 7.5
Temperature: 25
uniprot:P21643 [Hemin]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate, 100 µM ascorbate
PH: 7
Temperature: 37
uniprot:Q8PDA8 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 8
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 8
Temperature: 25
uniprot:Q8PDA8 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 8.3
Temperature: 25
uniprot:Q8PDA8 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 8.3
Temperature: 25
uniprot:Q8PDA8 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 8
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 6.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM phosphate
PH: 7
Temperature: 25
uniprot:P48775 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate
PH: 7.4
Temperature: 37
uniprot:P21643 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate
PH: 7.4
Temperature: 37
uniprot:G7P6G7 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate
PH: 7.4
Temperature: 37
uniprot:P48776 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate
PH: 7.4
Temperature: 37
uniprot:F1PGK8 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate
PH: 7.4
Temperature: 37
uniprot:A9V766 [Heme]

L_Tryptophan + O2 -> Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 8
Temperature: 37
uniprot:P48775 [Heme]

5_Methyltryptophan + O2 -> 5_Methyl_N_formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 µM methylene blue, 100 µg catalase, 20 mM L-ascorbate
PH: 8
Temperature: 25
uniprot:P48775 [Heme]

O2 + 5_Fluorotryptophan -> 5_Fluoro_N_formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 µM methylene blue, 100 µg catalase, 20 mM L-ascorbate
PH: 8
Temperature: 25
uniprot:P48775 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 µM methylene blue, 100 µg catalase, 20 mM L-ascorbate
PH: 8
Temperature: 25
uniprot:P48775 [Heme]

D_Tryptophan + O2 -> N_Formyl_D_kynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 µM methylene blue, 100 µg catalase, 20 mM L-ascorbate
PH: 8
Temperature: 25
uniprot:P48775 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7.4
Temperature: 22
uniprot:P48775 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7.4
Temperature: 22
uniprot:P48775 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 500 mM NaCl, 5 % glycerol, 500 µM L-ascorbate
PH: 8
Temperature: 25
uniprot:P48775 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 500 mM NaCl, 5 % glycerol, 500 µM L-ascorbate
PH: 8
Temperature: 25
uniprot:P48775 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 500 mM NaCl, 5 % glycerol, 500 µM L-ascorbate
PH: 8
Temperature: 25
uniprot:P48775 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 500 mM NaCl, 5 % glycerol, 500 µM L-ascorbate
PH: 8
Temperature: 25
uniprot:P48775 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 500 mM NaCl, 5 % glycerol, 500 µM L-ascorbate
PH: 8
Temperature: 25
uniprot:P48775 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 500 mM NaCl, 5 % glycerol, 500 µM L-ascorbate
PH: 8
Temperature: 25
uniprot:P48775 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 500 mM NaCl, 5 % glycerol, 500 µM L-ascorbate
PH: 8
Temperature: 25
uniprot:Q8PDA8 [Heme]

L_1_Methyltryptophan + O2 -> N_Formyl_methyl_L_kynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM phosphate, 10 µM methylene blue, 10 µg catalase, 20 mM sodium ascorbate
PH: 7.5
Temperature: 25
uniprot:Q8PDA8 [Heme]

L_1_Methyltryptophan + O2 -> N_Formyl_methyl_L_kynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM phosphate, 10 µM methylene blue, 10 µg catalase, 20 mM sodium ascorbate
PH: 7.5
Temperature: 25
uniprot:P48775 [Heme]

L_1_Methyltryptophan + O2 -> N_Formyl_methyl_L_kynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 µM methylene blue, 10 µg catalase, 20 mM sodium ascorbate
PH: 8
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_TRYPTOPHAN-DEGRADATION-1 tryptophan degradation
BioCyc:HUMAN_PWY66-401 superpathway of tryptophan utilization
BioCyc:META_PWY-5651 L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
BioCyc:MOUSE_NADSYN-PWY NAD biosynthesis II (from tryptophan)
BioCyc:MOUSE_TRPCAT-PWY tryptophan degradation I (via anthranilate)
BioCyc:HUMAN_PWY-6309 L-kynurenine degradation
BioCyc:META_NADSYN-PWY NAD de novo biosynthesis II (from tryptophan)
BioCyc:HUMAN_NADSYN-PWY NAD de novo biosynthesis
BioCyc:HUMAN_PWY-5651 tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
BioCyc:META_TRYPTOPHAN-DEGRADATION-1 L-tryptophan degradation III (eukaryotic)
BioCyc:META_PWY-6309 L-tryptophan degradation XI (mammalian, via kynurenine)
BioCyc:MOUSE_TRYPTOPHAN-DEGRADATION-1 tryptophan degradation III (eukaryotic)
BioCyc:MOUSE_PWY-5651 tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-DRE-71291 Amino acid and derivative metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-CEL-71240 Tryptophan catabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71240 Tryptophan catabolism
Reactome:R-MMU-71240 Tryptophan catabolism
Reactome:R-RNO-71240 Tryptophan catabolism
Reactome:R-XTR-71291 Amino acid and derivative metabolism
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DDI-71240 Tryptophan catabolism
Reactome:R-DME-71240 Tryptophan catabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-XTR-1430728 Metabolism
Reactome:R-XTR-71240 Tryptophan catabolism
Reactome:R-BTA-71240 Tryptophan catabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DRE-71240 Tryptophan catabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP465 Tryptophan metabolism
WikiPathways:WP5044 Kynurenine pathway and links to cell senescence
PathBank:SMP0087237 Tryptophan Metabolism
PathBank:SMP0000063 Tryptophan Metabolism