EC: 3.7.1.3

kynureninase (L-kynurenine hydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q16719 [Pyridoxal phosphate]

H2O + 3_Hydroxy_L_kynurenine -> 3_Hydroxyanthranilate + L_Alanine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris
PH: 8.5
Temperature: 37
uniprot:P70712 [Pyridoxal phosphate]

H2O + 3_Hydroxy_L_kynurenine -> 3_Hydroxyanthranilate + L_Alanine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris
PH: 8.5
Temperature: 37
uniprot:Q16719 [Pyridoxal phosphate]

L_Kynurenine + H2O -> Anthranilate + L_Alanine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris
PH: 8.5
Temperature: 37
uniprot:P70712 [Pyridoxal phosphate]

H2O + L_Kynurenine -> L_Alanine + Anthranilate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris
PH: 8.5
Temperature: 37
uniprot:Q16719 [Pyridoxal phosphate]

3_Hydroxykynurenine + H2O -> L_Alanine + 3_Hydroxyanthranilate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 10 mM Tris/HCl
PH: 7.9
Temperature: 37
- [Pyridoxal phosphate]

H2O + 3_Hydroxy_L_kynurenine -> 3_Hydroxyanthranilate + L_Alanine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 8
Temperature: 36
- [Pyridoxal phosphate]

H2O + L_Kynurenine -> L_Alanine + Anthranilate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 8
Temperature: 36
uniprot:P83788 [kynureninase(Enzyme) wildtype]

H2O + L_Kynurenine -> L_Alanine + Anthranilate		 (( (Vmax * A) ) / (Km + A)) buffer: 60 mM Tris-HCl, 0.05 mM pyridoxalphosphate, 5 mM NAD, 1.3 mg/ml Bacillus sphaericus alanine dehydrogenase
PH: 8
Temperature: 25
uniprot:P83788 [kynureninase(Enzyme) wildtype]

H2O + 3_Hydroxy_L_kynurenine -> 3_Hydroxyanthranilate + L_Alanine		 (( (Vmax * A) ) / (Km + A)) buffer: 60 mM Tris-HCl, 0.05 mM pyridoxalphosphate, 5 mM NAD, 1.3 mg/ml Bacillus sphaericus alanine dehydrogenase
PH: 8
Temperature: 25
uniprot:P83788 [kynureninase(Enzyme) wildtype]

H2O + L_Kynurenine -> L_Alanine + Anthranilate		 (( (Vmax * A) ) / (( (Km * (1 + (P / Ki))) ) + A)) buffer: 60 mM Tris-HCl, 0.05 mM pyridoxalphosphate, 5 mM NAD, 1.3 mg/ml Bacillus sphaericus alanine dehydrogenase
PH: 8
Temperature: 25
uniprot:P83788 [kynureninase(Enzyme) wildtype]

H2O + beta_Benzoyl_L_alanine -> L_Alanine + Benzoate		 (( (Vmax * A) ) / (Km + A)) buffer: 60 mM Tris-HCl, 0.05 mM pyridoxalphosphate, 5 mM NAD, 1.3 mg/ml Bacillus sphaericus alanine dehydrogenase
PH: 8
Temperature: 25
uniprot:P83788 [kynureninase(Enzyme) wildtype]

Formylkynurenine + H2O -> L_Alanine + Formylanthranilate		 (( (Vmax * A) ) / (Km + A)) buffer: 60 mM Tris-HCl, 0.05 mM pyridoxalphosphate, 5 mM NAD, 1.3 mg/ml Bacillus sphaericus alanine dehydrogenase
PH: 8
Temperature: 25
uniprot:P83788 [kynureninase(Enzyme) wildtype]

4_Fluoro_L_kynurenine + H2O -> L_Alanine + 4_Fluoroanthranilate		 (( (Vmax * A) ) / (Km + A)) buffer: 60 mM Tris-HCl, 0.05 mM pyridoxalphosphate, 5 mM NAD, 1.3 mg/ml Bacillus sphaericus alanine dehydrogenase
PH: 8
Temperature: 25
uniprot:P83788 [kynureninase(Enzyme) wildtype]

gamma_(o_Aminophenyl)_L_homoserine + H2O -> o_Aminobenzaldehyde + L_Alanine		 (( (Vmax * A) ) / (Km + A)) buffer: 60 mM Tris-HCl, 0.05 mM pyridoxalphosphate, 5 mM NAD, 1.3 mg/ml Bacillus sphaericus alanine dehydrogenase
PH: 8
Temperature: 25
uniprot:P83788 [kynureninase(Enzyme) wildtype]

5_Fluoro_L_kynurenine + H2O -> L_Alanine + 5_Fluoroanthranilate		 (( (Vmax * A) ) / (Km + A)) buffer: 60 mM Tris-HCl, 0.05 mM pyridoxalphosphate, 5 mM NAD, 1.3 mg/ml Bacillus sphaericus alanine dehydrogenase
PH: 8
Temperature: 25
uniprot:P83788 [beta-Benzoyl-L-alanine]

L_Kynurenine + H2O -> Anthranilate + L_Alanine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 0.04 M potassium phosphate, 40 µM pyridoxal 5'-phosphate
PH: 7.8
Temperature: 25
uniprot:P83788 [kynureninase(Enzyme) wildtype]

L_Kynurenine + H2O -> L_Alanine + Anthranilate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.04 M potassium phosphate, 40 µM pyridoxal 5'-phosphate
PH: 7.8
Temperature: 25
uniprot:P83788 [kynureninase(Enzyme) wildtype]

beta_Benzoyl_L_alanine + H2O -> L_Alanine + Benzoate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.04 M potassium phosphate, 40 µM pyridoxal 5'-phosphate
PH: 7.8
Temperature: 25
uniprot:P70712 [Pyridoxal phosphate]

L_Kynurenine + H2O -> Anthranilate + L_Alanine		 (( (Vmax * S) ) / (Km + S)) buffer: 90 mM Tris-HCl
PH: 8.7
Temperature: 37
uniprot:P70712 [Pyridoxal phosphate]

L_Kynurenine + H2O -> L_Alanine + Anthranilate		 (( (Vmax * S) ) / (Km + S)) buffer: 90 mM Tris-HCl
PH: 7.7
Temperature: 37
uniprot:P70712 [Pyridoxal phosphate]

H2O + 3_Hydroxykynurenine -> 3_Hydroxyanthranilate + L_Alanine		 (( (Vmax * S) ) / (Km + S)) buffer: 90 mM Tris-HCl
PH: 7.7
Temperature: 37
uniprot:P70712 [Pyridoxal phosphate]

H2O + 3_Hydroxykynurenine -> 3_Hydroxyanthranilate + L_Alanine		 (( (Vmax * S) ) / (Km + S)) buffer: 90 mM Tris-HCl
PH: 8.7
Temperature: 37
uniprot:P70712 [Pyridoxal phosphate]

H2O + L_Kynurenine -> Anthranilate + L_Alanine		 (( (Vmax * S) ) / (Km + S)) buffer: 90 mM Tris-HCl
PH: 7.7
Temperature: 37
uniprot:P70712 [Pyridoxal phosphate]

L_Kynurenine + H2O -> L_Alanine + Anthranilate		 (( (Vmax * S) ) / (Km + S)) buffer: 90 mM Tris-HCl
PH: 8.7
Temperature: 37
uniprot:P70712 [Pyridoxal phosphate]

3_Hydroxykynurenine + H2O -> L_Alanine + 3_Hydroxyanthranilate		 (( (Vmax * S) ) / (Km + S)) buffer: 90 mM Tris-HCl
PH: 8.7
Temperature: 37
uniprot:P70712 [Pyridoxal phosphate]

3_Hydroxykynurenine + H2O -> L_Alanine + 3_Hydroxyanthranilate		 (( (Vmax * S) ) / (Km + S)) buffer: 90 mM Tris-HCl
PH: 7.7
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_TRYPTOPHAN-DEGRADATION-1 tryptophan degradation
BioCyc:HUMAN_PWY66-401 superpathway of tryptophan utilization
BioCyc:META_PWY-6954 superpathway of aromatic compound degradation via 2-hydroxypentadienoate
BioCyc:META_PWY-3502 superpathway of NAD biosynthesis in eukaryotes
BioCyc:META_PWY-5651 L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
BioCyc:YEAST_PWY-5651 tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
BioCyc:MOUSE_NADSYN-PWY NAD biosynthesis II (from tryptophan)
BioCyc:MOUSE_TRPCAT-PWY tryptophan degradation I (via anthranilate)
BioCyc:HUMAN_PWY-6309 L-kynurenine degradation
BioCyc:META_PWY-2504 superpathway of aromatic compound degradation via 3-oxoadipate
BioCyc:META_NADSYN-PWY NAD de novo biosynthesis II (from tryptophan)
BioCyc:YEAST_NADSYN-PWY NAD de novo biosynthesis
BioCyc:ANTHRA_PWY-5651 L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
BioCyc:ANTHRA_TRPCAT-PWY L-tryptophan degradation I (via anthranilate)
BioCyc:HUMAN_NADSYN-PWY NAD de novo biosynthesis
BioCyc:HUMAN_PWY-5651 tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
BioCyc:META_TRPCAT-PWY L-tryptophan degradation I (via anthranilate)
BioCyc:META_TRYPTOPHAN-DEGRADATION-1 L-tryptophan degradation III (eukaryotic)
BioCyc:META_PWY-6309 L-tryptophan degradation XI (mammalian, via kynurenine)
BioCyc:MOUSE_TRYPTOPHAN-DEGRADATION-1 tryptophan degradation III (eukaryotic)
BioCyc:MOUSE_PWY-5651 tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SCE-71291 Amino acid and derivative metabolism
Reactome:R-CEL-71240 Tryptophan catabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71240 Tryptophan catabolism
Reactome:R-MMU-71240 Tryptophan catabolism
Reactome:R-RNO-71240 Tryptophan catabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-DDI-71240 Tryptophan catabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SCE-71240 Tryptophan catabolism
WikiPathways:WP5044 Kynurenine pathway and links to cell senescence
PathBank:SMP0002353 Tryptophan Metabolism
PathBank:SMP0000063 Tryptophan Metabolism
PathBank:SMP0002338 NAD Metabolism
PathBank:SMP0087451 Tryptophan Metabolism