uniprot:Q9NV35 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype NUDT15]
dGTP + H2O -> dGMP + Diphosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-acetate, 40 mM NaCl, 10 mM MgAc, 1 mM dithiothreitol, 0.005% Tween-20
PH: 7.5
Temperature: 22 |
uniprot:Q9NV35 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype NUDT15]
H2O + 8_oxo_dGTP -> 8_oxo_dGMP + Diphosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-acetate, 40 mM NaCl, 10 mM MgAc, 1 mM dithiothreitol, 0.005% Tween-20
PH: 7.5
Temperature: 22 |
uniprot:P36639 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype MTH1]
dGTP + H2O -> dGMP + Diphosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-acetate, 40 mM NaCl, 10 mM MgAc, 1 mM dithiothreitol, 0.005% Tween-20
PH: 7.5
Temperature: 22 |
uniprot:P36639 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype MTH1]
H2O + 8_oxo_dGTP -> Diphosphate + 8_oxo_dGMP |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-acetate, 40 mM NaCl, 10 mM MgAc, 1 mM dithiothreitol, 0.005% Tween-20
PH: 7.5
Temperature: 22 |
uniprot:Q8BG93 [Mg2+]
H2O + dGTP -> Diphosphate + dGMP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 80 µg/ml bovine serum albumin, 40 mM NaCl, 5 mM DTT, 2 % glycerol
PH: 8
Temperature: 30 |
uniprot:Q8BG93 [Mg2+]
H2O + 8_oxo_dGTP -> Diphosphate + 8_oxo_dGMP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 80 µg/ml bovine serum albumin, 40 mM NaCl, 5 mM DTT, 2 % glycerol
PH: 8
Temperature: 30 |
uniprot:Q9BY32 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype]
XTP + H2O -> Diphosphate + XMP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 50 mM MgCl2, 1 mM Dithiothreitol, 1.25 U/ml Inorganic Pyrophosphatase
PH: 8.5
Temperature: 37 |
uniprot:Q9BY32 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype]
ITP + H2O -> IMP + Diphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 50 mM MgCl2, 1 mM Dithiothreitol, 1.25 U/ml Inorganic Pyrophosphatase
PH: 8.5
Temperature: 37 |
uniprot:Q9BY32 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype]
dITP + H2O -> dIMP + Diphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 50 mM MgCl2, 1 mM Dithiothreitol, 1.25 U/ml Inorganic Pyrophosphatase
PH: 8.5
Temperature: 37 |
uniprot:Q924C3 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype isoenzyme Enpp1]
H2O + UDPglucose -> UMP + D_Glucose_1_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 8 µg/ml membrane protein
PH: 8.9
Temperature: 25 |
uniprot:Q924C3 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype isoenzyme Enpp1]
UTP + H2O -> Diphosphate + UMP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 8 µg/ml membrane protein
PH: 8.9
Temperature: 25 |
uniprot:Q924C3 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype isoenzyme Enpp1]
p_Nitrophenylthymidine_phosphate + H2O -> p_Nitrophenol + dTMP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 0.2 µg/ml membrane protein
PH: 8.9
Temperature: 25 |
- [nucleoside-triphosphate diphosphatase(Enzyme) wildtype]
H2O + p_Nitrophenylthymidine_phosphate -> dTMP + p_Nitrophenol |
(( (V * S) ) / (Km + S)) |
|
buffer: 100 mM glycine-NaOH, 5 mM MgCl2, 100 µM ZnCl2
PH: 9.6
Temperature: 20 |
- [nucleoside-triphosphate diphosphatase(Enzyme) wildtype]
H2O + p_Nitrophenylthymidine_phosphate -> p_Nitrophenol + dTMP |
(( (V * S) ) / (Km + S)) |
|
buffer: 100 mM glycine-NaOH, 5 mM MgCl2, 100 µM ZnCl2
PH: 9.6
Temperature: 20 |
uniprot:P97675 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype]
H2O + NAD+ -> AMP + Nicotinamide_nucleotide |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M triethanolamine, 0.1 mM ATP, 0.2 mM NADH, 10 mM MgCl2, 1 mM phosphoenol pyruvate, 0.1 M potassium chloride, 3.5 U/ml myokinase, 1.5 U/ml pyruvate kinase, 3.5 U/ml lactate dehydrogenase
PH: 7.6
Temperature: 37 |
uniprot:P97675 [ADPribose]
NADH + H2O -> AMP + Reduced_nicotinamide_nucleotide |
(( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) |
|
buffer: 0.1 M Tris, 10 mM MgCl2
PH: 9
Temperature: 37 |
uniprot:P97675 [UDPglucose]
NADH + H2O -> Reduced_nicotinamide_nucleotide + AMP |
(( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) |
|
buffer: 0.1 M Tris, 10 mM MgCl2
PH: 9
Temperature: 37 |
uniprot:P97675 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype]
UDPglucose + H2O -> UMP + D_Glucose_1_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M triethanolamine, 0.1 mM ATP, 0.2 mM NADH, 10 mM MgCl2, 1 mM phosphoenol pyruvate, 0.1 M potassium chloride, 3.5 U/ml myokinase, 1.5 U/ml pyruvate kinase, 3.5 U/ml lactate dehydrogenase
PH: 7.6
Temperature: 37 |
uniprot:P97675 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype]
NADH + H2O -> AMP + Reduced_nicotinamide_nucleotide |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Tris, 10 mM MgCl2
PH: 9
Temperature: 37 |
uniprot:P97675 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype]
ADPribose + H2O -> AMP + D_Ribose_1_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M triethanolamine, 0.1 mM ATP, 0.2 mM NADH, 10 mM MgCl2, 1 mM phosphoenol pyruvate, 0.1 M potassium chloride, 3.5 U/ml myokinase, 1.5 U/ml pyruvate kinase, 3.5 U/ml lactate dehydrogenase
PH: 7.6
Temperature: 37 |
uniprot:Q924C3 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype isoenzyme Enpp1]
H2O + ATP -> AMP + Diphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 8 µg/ml membrane protein
PH: 8.9
Temperature: 25 |
uniprot:Q924C3 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype isoenzyme Enpp1]
H2O + ADP -> AMP + Phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 8 µg/ml membrane protein
PH: 8.9
Temperature: 25 |
uniprot:Q924C3 [UMP]
p_Nitrophenylthymidine_phosphate + H2O -> p_Nitrophenol + dTMP |
(( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 0.2 µg/ml membrane protein
PH: 8.9
Temperature: 25 |
uniprot:Q924C3 [alpha,beta-Methylene ATP]
H2O + p_Nitrophenylthymidine_phosphate -> p_Nitrophenol + dTMP |
(( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 0.2 µg/ml membrane protein
PH: 8.9
Temperature: 25 |
uniprot:Q924C3 [UDPglucose]
p_Nitrophenylthymidine_phosphate + H2O -> dTMP + p_Nitrophenol |
(( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 0.2 µg/ml membrane protein
PH: 8.9
Temperature: 25 |
uniprot:Q924C3 [Adenosine 5'-phosphoramidate]
H2O + p_Nitrophenylthymidine_phosphate -> dTMP + p_Nitrophenol |
(( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 0.2 µg/ml membrane protein
PH: 8.9
Temperature: 25 |
uniprot:Q924C3 [nucleoside-triphosphate diphosphatase(Enzyme) wildtype isoenzyme Enpp1]
H2O + UDP -> Phosphate + UMP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 8 µg/ml membrane protein
PH: 8.9
Temperature: 25 |